Effects of Herbal Extracts Used in Oriental Medicines on Heme Oxygenase-1 Expression

Jeong, Gil-Saeng,Oh, Seung-Hwan,Kang, Dae-Gill,Lee, Ho-Sub,Kim, Youn-Chul The Physiological Society of Korean Medicine and T 2006 동의생리병리학회지 Vol.20 No.5

Effects of twenty-three aqueous herbal extracts used in oriental medicines on heme oxygenase (HO)-1 expression were estimated in a mouse hippocampal cell line, HT22. HO-1 is one of the cytoprotective enzymes activated various stimuli, and Western blot analysis was used for evaluated HO-1 expression. Six aqueous extracts such as Rhei Rhizoma, Paeoniae Radix, Uncariae Ramulus et Uncus, Theae Folium, Prunellae Spica, and Coptidis Rhizoma significantly increased HO-1 expression in HT22 cells at the concentration of 300 ${\mu}$g/ml. In Addition, four aqueous extracts including Eucommiae Cortex, Moutan Cortex Radicis, Ginseng Radix Rubra, and Scutellariae Radix moderately increased HO-1 expression. These results would be usefulfor the isolation and identification of their neuroprotective principles.

Sauchinone Suppresses Pro-inflammatory Mediators by Inducing Heme Oxygenase-1 in RAW264.7 Macrophages

Li, Bin,Lee, Dong-Sung,Choi, Hyun-Gyu,Kim, Kyoung-Su,Kang, Dae-Gil,Lee, Ho-Sub,Jeong, Gil-Saeng,Kim, Youn-Chul Pharmaceutical Society of Japan 2011 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.34 No.10

<P>Sauchinone, a biologically active lignan isolated from the roots of <I>Saururus chinensis</I> (L<SMALL>OUR</SMALL>.) B<SMALL>AILL</SMALL>. (Saururaceae), is reported to exert a variety of biological activities, such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to a reduction in COX-2-derived prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) and iNOS-derived nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. Present study also demonstrates the effects of sauchinone in inducing heme oxygenase-1 (HO-1) expression and an increase in heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sauchinone on LPS-induced PGE<SUB>2</SUB>, NO, tumor necrosis factor-α (TNF-α) and interlukine-1β (IL-1β) production were partially reversed by the HO-1 inhibitor Tin protoporphyrin was also seen in this study. In addition, we found that treatment with extracellular signal-regulated kinase (ERK) inhibitor (PD98059) reduced sauchinone-induced HO-1 expression. Sauchinone also increased ERK phosphorylation. These results suggest that sauchinone inhibits pro-inflammatory mediators through expression of anti-inflammatory HO-1 <I>via</I> ERK pathway.</P>

키넥트 센서를 이용한 고령자 대상의 선자세 균형능력 평가

양승태(Seung-Tae Yang),강동원(Dong-Won Kang),서정우(Jeong-Woo Seo),김대혁(Dae-Hyeok Kim),김태호(Tae-Ho Kim),최진승(Jin-Seung Choi),탁계래(Gye-Rae Tack) 대한전기학회 2017 전기학회논문지 Vol.66 No.2

Portable low-cost Kinect sensor was used to analyze standing balance ability of the elderly. Eighty subjects who can walk alone and have a normal cognitive level participated in this experiment. Based on Berg Balance scale (BBS) test with 52 points, subjects were divided into Healthy older (HO: 46 persons, BBS: 53.80 ± 1.19) and Impaired older (IO: 34 persons, BBS: 49.06 ± 2.03) group. Each subject performed 30 seconds four different standing balance tests (EO: Eyes Open, EC: Eyes Close, EOf: Eyes Open on foam, ECf: Eyes Close on foam). Five variables (Mean distance, Range of distance, Root mean square, Mean velocity, 95% ellipse area) were calculated from the hip joint center movement of Kinect sensor. Results showed that there were significant differences between groups for four different standing tests. Calculated variables from kinect sensor showed significant correlation with BBS score. Especially, mediolateral mean distance, mediolateral root mean square, mediolateral range of distance and 95% ellipse area showed discriminative ability for all tests. Mean values of variables of IO were higher than those of HO, which means the decreased balance ability in IO compared with HO. Therefore, it was possible to estimate simple balance assessment of the elderly using portable low-cost Kinect sensor.

뇌졸중 환자의 우울증에 영향을 미치는 요인

김대용,정주호 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: The diseases are getting increasingly chronic as the fast development of medical and scientific technical advances prolong the human life span. Among many chronic diseases, the apoplexy leads to partial paralysis, speech disorder, perception disorder and paresthesia and motor disturbance. It also causes mental disorder to patients. This research was aimed to check the level of stress perceived by apoplexy patients according to the socioeconomic factors and disease characteristics. Method: Applying the Beck's depression measurement, we surveyed the depression level of 150 inpatients and outpatients who have been currently taken care of by the departments of neurology, neurosurgery and rehabilitation at a university hospital and two general hospitals in Busan. Results: According to this survey, 84 out of 150 patients are estimated to be depressed, accounting 56% of the total samples. The depression level related to the socioeconomic factors is higher in patients who are older, separated from spouse and who have low education level and financial dependence on offsprings. The depression level related to the neurological manifestations is higher in patients who have multiple strokes, long term illness, bilateral paralysis, and other neurological sequelae (particularly gait disturbance). Conclusion: The result suggest that poststroke patients are highly likely to suffer from depression by various socioeconomic factors and neurological manifestations. Therefore, it can be concluded that poststroke patients need not only medical treatments and rehabilitation procedures but also more proactive mental remedies and social supports considering patient's backgrounds and characteristics.

Chloroform 만성 중독으로 인한 간병변에 대한 병리학적 연구

정호석,강대영 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1

In an attempt to elucidate the pathological effects of chloroform(CHCl_(3)) administration, the present study was undertaken in male Sprague-Dawley rats. Evidence for the existence of hyperplastic nodules, oval cells, bile duct or ductular proliferation, and liver cirrhosis was examined, and the number of dysplastic cells were evaluated, emphasizing especially on the difference among the several groups. Rats were administered 300mg/kg or 600mg/kg of chloroform(CHCl_(3)-corn oil 1:4 solution) intraperitoneally, and then twice injected weekly for 36 weeks. The control animals were given as similar volume of saline or corn oil, respectively. The animals were sacrificed in 3rd, 6th and 9th month after chloroform injection. The histopathologic changes in the liver of the control and experimenal groups were noted as follows: 1) The hyperplastic nodules in the 600 mg/kg chloroform treated group were more prominent than those of the 300 mg/kg chloroform treated group in the number and size. 2) The number of dysplastic hepatocytes were increased gradually as the experiment continiued. The incidence of dysplastic hepatocytes was 45.5% in the 300 mg/kg treated group and 70.6% in the 600 mg/kg treated group. 3) Oval cells appeared in the early days of the experiment (3rd month) and then gradually decreased in number. 4) The degree of severity of liver cirrhosis and bile duct or ductular proliferation gradually increased as the experiment continued. Incidence of liver cirrhosis and bile duct or ductular proliferation was 31.8% in the 300 mg/kg treated group and 64.7% in the 600 mg/kg treated group, respectivley. 5) Incidence of the chloroform induced hepatoma was 5.9%(1 out of 17 rats) in the chloroform 600 mg/kg treated group, and the hepatoma spread to the liver, followed by metastasis to the omentum and ileum. 6) Light microscopically, some hepatic lobules revealed some foci of vacuolated or clear hepatocytes. Electron microscopically, they contained fat droplets. Others also contained increased number of mitochondria or distorted mitochondria. In summary, the result obtained by the present study indicates chloroform induced hyperplastic nodules, liver cell dysplasia, oval cell proliferation and liver cirrhosis, and secondarily induced hepatoma.

Multiple Spinal Intradural Schwannomas Combined with Sequestered Lumbar Disc Herniation

김대용,김진욱,정주호,조재훈 고신대학교 의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

In this report, we describe a case of multiple intradural schwannomas in the spine combined with a upward sequestered disc herniation at L4-L5. It is often difficult to differentiate this tumor from lumbar disc herniation, especially a sequestered hernia. Gadolinium-enhanced MR images were a useful preoperative examination modality for differentiating these lesions from other diseases. Microscopically, the intradural tumors were successfully removed. The dura maters of the L2 and S1 were opened microsurgically, allowing the nerve fibers involved in the tumor to be identified. The involved fibers were cut around the tumor, and the lesion was resected while the intact nerve fibers were preserved. An upward sequestered lumbar disc herniation at L4-L5 was also successfully removed. Based on histological examination of the resected specimen, the tumors were diagnosed as schwannomas. Microsurgery allowed the tumors to be removed with minimal impairment from cutting of nerve fibers in the each nerve root.

플라즈마 표면복합처리한 구상흑연주철의 열피로 특성

정봉용,주대헌,박주승,김명호 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.9

This paper reports an investigation of the characteristics of plasma duplex treated ductile cast irons during thermal fatigue tests on a thermal cycling rig. The thermal fatigue cracking behaviors and microstructure of untreated and plasma surface treated ductile cast irons have been investigated. Both ferrite and pearlite matrix ductile cast irons were evaluated. During the tests, 60 mm long cylinders with 20 mm diameter were subjected to 1,000 high frequency induction heating and water cooling cycles, lasting 7 and 5 sec, respectively. The thermal fatigue damage was evaluated by analyzing different crack dimensions and distribution by light microscopy. The results showed that the plasma surface treatment increased thermal fatigue cracking resistance. When thermal fatigue cracking occurs, the cracks always initiate at the surface of the specimen. The major path of crack propagation is generally along the ferrite grain boundaries. The hardened layer had an effect on the thermal fatigue property. In addition, the ductile cast iron with an pearlite matrix has a better resistance to thermal fatigue than that of ferrite matrix.

중증외상환자에서 동반된 척추 및 척수손상에 대한 임상적 분석

정주호,김대용,박창민 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: The traumatic spinal injury is an extremely serious condition that often results in death or disabilities. We investigated epidemiological data on spinal injuries specifically in severe trauma patient admitted to a preliminarily proposed Major Trauma Distinctive Care Center. Materials and Methods: We reviewed the medical records of 28 severe trauma patients (Injury Severity Score >15) with traumatic spinal (vertebral column or spinal cord) injuries admitted to the emergency medical center in the Kosin University Gospel Hospital (KUGH) from January to October 2009, retrospectively. Results: The ratio of male to female was 3 to 1. The mean age was 46.5 years. The leading causes of traumatic spinal injury are falls (39.4%). The most common level of injury was cervical (35%) and lumbar (35%) spine. Spinal fracture accounts for 47.8% of all traumatic spinal injury, spinal cord injury 37%, dislocation 8.7% and HNP (herniated nucleus pulposus) 6.5%. The most common type of spinal body fracture was compressive type (58.8%). In spinal cord injury, incomplete injury (64.7%) was more common than complete injury (35.3%) and quadriplegia accounts for 47.1% and paraplegia 52.9%. The most common associated injury was rib fracture & pneumohemothorax (31.2%). 5 patients underwent surgery earlier than 24 hours. The average Injury Severity Score (ISS) was 24.2 and the average Abbreviated Injury Scale Score (AISS) was 3.5. Conclusion: This clinical analysis is the first basic study for understanding the patterns of the spinal injuries in the severe trauma patients admitted to KUGH emergency medical center which was preliminarily proposed as Major Trauma Distinctive Care Center by Ministry for Health, Welfare and Family Affairs in November 2008. Although this study has only small number of cases during relatively short term period, it will be touchstone of succeeding analysis of spinal injuries in the severe trauma patients. Therefore continuous additional data collections and more precise clinical investigations are suggested

韓國産 가지과 植物에 관한 本草學的 硏究

정대기,변종호,신민교,송호준 대한본초학회 1998 大韓本草學會誌 Vol.13 No.1

For the purpose of developing Korean Herbalogy with the plants of Solanceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been throughly investigative and the results obtained were as follows: 1. There were totaled to 13 genera and 26 species in Korea and among them medicinal plants are 11 genera, 19 species, some 73% in total but, the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Solanaceae family were classified as Radix 8, Fructus 8, Herba 6 ,Foilum 5, Semen 3, Lignum and Flos 2. 3. According to sum of 26 species in Solanaceae family, they were classified into Solanum genera 8, Physalis and Capsicum genera 3 each, Datura and Nicotiana genera 2 each. Thus it was noticed that Solanum genera was the main kind, some 30% in total. 4. According to the number of species of the origin plants about each chinese materia medicals, they were calssified into HERBA SOLANI LYRATI 3, FRUCTUS CAPSICI and FLOS DATURAE 2 each. 5. According to nature and flavour of medicinal plants, they were classified into cold, cool; 17 each,warmth,heat; 8 each, balance 5 and unidentified 6. Thus it was noticed that cold and cool is the main in nature and flavour of medicinal plants. 6. According to the Properties and Principal curative action, they were classified into Drugs for treating febrifugal and detoxicant 14, Drugs for relieving pain 7, Antitussives . Diuretics . Drugs for invigorating blood circulation. Tonics 4 each, Anthelmintics 3. 7. Comparing to whole medicinal plants 19 kinds, toxic drugs include minor toxin were 3 kinds, 16% of the whole. From this result, It was revealed that the plants for medical purpose in Solanaceae was 73% of the whole, in which RADIX and FRUCTUS were abundunt. It is considered that many experiments and clinical approaches must be continued to use Solanaceae plants widely.

Phenobarbital 및 인진호투여가 四鹽化炭素에 依한 急性 中毒性 肝病變에 미치는 影響에 關한 病理組織學的 硏究

鄭昊錫,姜大榮 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

Carbon tetrachloride, a potent hepatotoxic agent, is mainly metabolized in the liver, and its hepatotoxic action is influenced by the administration of phenobarbital or artemisia messerschimidtiana besser var. viridis(artemisia). Artemisia was used as folk medicine for the treatment of hyperbilirubinemia or patient with acute hepatitis. Sprague-Dawley rats(male and female) were administrated with 2 ml of carbon tetrachloride (CCI_4-olive oil 1 : 4 solution) intraperitoneally, after being anesthetized by ether. After 24 hours, phenobarbital(50 mg/kg/day) and artemisia (0.6 ml/kg/day) were admin istrated intraperitoneally for 3 days. Food was withheld 12 hours before CCI_4, administration. 7 animals of each group were sacrificed at 4th, 5th, 6th and 7 th day after CCI_4 injection. The histopathological changes in the liver cells of the control and experimental groups were noted as follows; 1. Vacuolar degeneration of the hepatic cells of CCl_4-phenobarbital administrated rats showed more marked in degree than the control, and that of CCl_4-artemisia administrated rats is milder than the control. 2. Fatty change of the hepatic cells of CCl_4,-phenobarbital administrated rats showed more marked in degree than the control, and that of CCl_4-artemisia administrated rats is milder than the control. 3. The necrotic changes of the hepatic cells of CCl_4,-phenobarbital administrated rats showed more marked in degree and remained longer than the control. Temporarily, that of CCl_4-artemisia administrated rats showed more marked in degree than control, but early recovered.