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Lee, Dongtak,Lee, Hyungbeen,Lee, Gyudo,Kim, Insu,Lee, Sang Won,Kim, Woong,Lee, Sang Woo,Lee, Jeong Hoon,Park, Jinsung,Yoon, Dae Sung IOP 2019 Nanotechnology Vol.30 No.8
<P>With the rapid development of nanotechnology and its associated waste stream, public concern is growing over the potential toxicity exposure to heavy metal ions poses to the human body and the environment. Herein, we report an extremely sensitive Kelvin probe force microscopy (KPFM)-based platform for detecting nanotoxic materials (e.g. Ag<SUP>+</SUP>) accomplished by probing the integrated surface potential differences of a single gold nanoparticle on which an interaction between probe DNA and target DNA occurs. This interaction can amplify the surface potential of the nanoparticle owing to the coordination bond mediated by Ag<SUP>+</SUP> (cytosine–Ag<SUP>+</SUP>–cytosine base pairs). Interestingly, compared with conventional methods, this platform is capable of extremely sensitive Ag<SUP>+</SUP> detection (∼1 fM) in a remarkably wide-range (1 fM to 1 <I>μ</I>M). Furthermore, this platform enables Ag<SUP>+</SUP> detection in a practical sample (general drinking water), and this KPFM-based technique may have the potential to detect other toxic heavy metal ions and single nucleotide polymorphisms by designing specific DNA sequences.</P>
Lee, Myunggyo,Lee, Kyubum,Yu, Namhee,Jang, Insu,Choi, Ikjung,Kim, Pora,Jang, Ye Eun,Kim, Byounggun,Kim, Sunkyu,Lee, Byungwook,Kang, Jaewoo,Lee, Sanghyuk Oxford University Press 2017 Nucleic acids research Vol.45 No.d1
<P>Fusion gene is an important class of therapeutic targets and prognostic markers in cancer. ChimerDB is a comprehensive database of fusion genes encompassing analysis of deep sequencing data and manual curations. In this update, the database coverage was enhanced considerably by adding two new modules of The Cancer Genome Atlas (TCGA) RNA-Seq analysis and PubMed abstract mining. ChimerDB 3.0 is composed of three modules of ChimerKB, ChimerPub and ChimerSeq. ChimerKB represents a knowledgebase including 1066 fusion genes with manual curation that were compiled from public resources of fusion genes with experimental evidences. ChimerPub includes 2767 fusion genes obtained from text mining of PubMed abstracts. ChimerSeq module is designed to archive the fusion candidates from deep sequencing data. Importantly, we have analyzed RNA-Seq data of the TCGA project covering 4569 patients in 23 cancer types using two reliable programs of FusionScan and TopHat-Fusion. The new user interface supports diverse search options and graphic representation of fusion gene structure. ChimerDB 3.0 is available at http://ercsb.ewha.ac.kr/fusiongene/.</P>
Lee JeongEun,Lee Jean,Jeon Sungwon,Lee Jeongha,Jang Insu,Yang Jin Ok,Park Soojin,이병욱,Choi Jinwook,Choi Byung-Ok,Gee Heon Yung,Oh Jaeseong,Jang In-Jin,Lee Sanghyuk,Baek Daehyun,Koh Youngil,Yoon Sung-So 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Despite substantial advances in disease genetics, studies to date have largely focused on individuals of European descent. This limits further discoveries of novel functional genetic variants in other ethnic groups. To alleviate the paucity of East Asian population genome resources, we established the Korean Variant Archive 2 (KOVA 2), which is composed of 1896 whole-genome sequences and 3409 whole-exome sequences from healthy individuals of Korean ethnicity. This is the largest genome database from the ethnic Korean population to date, surpassing the 1909 Korean individuals deposited in gnomAD. The variants in KOVA 2 displayed all the known genetic features of those from previous genome databases, and we compiled data from Korean-specific runs of homozygosity, positively selected intervals, and structural variants. In doing so, we found loci, such as the loci of ADH1A/1B and UHRF1BP1, that are strongly selected in the Korean population relative to other East Asian populations. Our analysis of allele ages revealed a correlation between variant functionality and evolutionary age. The data can be browsed and downloaded from a public website (https://www.kobic.re.kr/kova/). We anticipate that KOVA 2 will serve as a valuable resource for genetic studies involving East Asian populations.
Microwave-induced formation of oligomeric amyloid aggregates
Lee, Wonseok,Choi, Yeseong,Lee, Sang Won,Kim, Insu,Lee, Dongtak,Hong, Yoochan,Lee, Gyudo,Yoon, Dae Sung IOP 2018 Nanotechnology Vol.29 No.34
<P>Amyloid aggregates have emerged as a significant hallmark of neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. Although it has been recently reported that microwave heating induces amyloid aggregation compared with conventional heating methods, the mechanism of amyloid aggregate induction has remained unclear. In this study, we investigated the formation of oligomeric amyloid aggregates (OAAs) by microwave irradiation at microscale volumes of solution. Microwave irradiation of protein monomer solution triggered rapid formation of OAAs within 7 min. We characterized the formation of OAAs using atomic force microscopy, thioflavin T fluorescent assay and circular dichroism. In the microwave system, we also investigated the inhibitory effect on the formation of amyloid aggregates by L-ascorbic acid as well as enhanced amyloid aggregation by silver nanomaterials such as nanoparticles and nanowires. We believe that microwave technology has the potential to facilitate the study of amyloid aggregation in the presence of chemical agents or nanomaterials.</P>
이인수(Lee, InSu),이재원(Lee, JaeOne),손종영(Lee, JaeOne),김수정(Kim, SuJeong) 한국지적학회 2011 한국지적학회 학술대회 논문집 Vol.2011 No.9
본 연구에서는 지적측량을 위한 항공사진측량의 국내외 활용 사례를 조사하고 디지털 항공카메라를 이용한 사진측량의 지적측량에의 적용방안을 제시하고자 하였다. 항공사진측량은 지형도제작 등에서는 오래전부터 사용되었지만 지적측량에서는 상대적으로 드물게 활용되고 있다. 최근 디지털 항공카메라의 개발과 정보기술의 발달로 고해상 영상취득이 가능해지고 있지만 지적측량에의 적용을 위해서 우선 법제도적 문제, 항측기술을 이용한 표준 지적 경계추출 프로세스 등이 마련되어야 하며 향후 지적정보와 항공사진의 영상정보를 융합하여 공간정보산업을 활성화 시킬 수 있는 방안이 마련되어야 할 것으로 사료된다. The purpose of this study is to investigate the application of aerial photogrammetry for cadastral surveying at home and overseas and propose ways to apply it for cadastral surveying with digital aerial photogrammetry camera. Actually, aerial photogrammetry had been applied for topographical mapping as a standard technology long time ago, but it had not been mature for cadastral surveying relatively. Recently, the advancement of digital aerial photogrammetry camera and the development of Information Technology encourage to capture the high resolution imagery, but for the actual applying it for cadastral surveying, the law and the institutional problems, standard procedures for cadastral boundaries' extraction with aerial photogrammetry should be arranged in advance. Hence, the fusion method of aerial photo with cadastral information to revitalize the spatia information industry should be actively considered.