Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

후방골절단술과 Skeletal anchorage system를 이용한 사악구치부압하 : 증례보고 CASES REPORT

임광수,풍무걸,김기형,이희철,김우형,김동우 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

The severe extrusion of the maxillary [post.teeth after the early loss of the mandibular post.teeth makes the fabrication of the mandibular prothesis difficult, and without proper planning.the treatment results are often unsatisfactoy. Several treatment modalitys have been introducted to correct the interarch space deficency.:Reduction the occlusal surfaces,Crown lengthening,Endodontics & restorative approach,Extraction, Orthodontic intrusion and segmental osteotomy. Because of many advantages,conservative method is widely used if it has no particular limits of treatment adjustment, but the cases of surgical correction indication are found in clinical experience. In our clinic,we corrected severe extruded maxillary post.teeth by performance of a unilateral posterior osteotomy with superior repositioning of the segment and orthodontic intrusion using Skeletal anchorage system,so here we reported the cases with a study of documents.

환경의식의 종합적 측정을 위한 설문지 개발

우형택,문연화 대구효성가톨릭대학교 1998 연구논문집 Vol.58 No.2

With respect to the increasing importance of understanding our environmental concerns, this study was conducted to develop the questionnaire for the comprehensive and objective measurement of environmental concerns and test its reliability. The major research methods taken include the design of environmental questionnaire based on literature review and related theories, and the process of increasing the reliability of developed questionnaire through the repetition of reliability analysis. The total of 3 surveys were conducted and the total of 510 persons including diverse sample groups were questionnaired. The SAS PC+ package program was used in the reliability analysis of surveyed data. The first designed questionnaire consisted of 50 items including 25 items related to environmental pollution and 25 items related to nature conservation, reflecting various factors of environmental concerns. The result of the first reliability analysis showed that Cronbach-αfor the total was 0.75. To increase the reliability, items having low correlation with the total were eliminated, corrected, or revised. The reliability of redesigned questionnaire consisting of 46 items was significantly improved to the level of Cronbach-α0.88, the high reliability of measurement. In the second reliability analysis, 11 items were further revised and corrected to develop the questionnaire having the very high reliability. The reliability of the finalized questionnaire was further increased to the level of Cronbach-α0.92.

A Study on Protection of Maternal Antibody against Hantavirus in Rats

우영대,--,--,--,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The etiologic agents of haemorragic fever with ranal syndrom (HFRS) in Korea are Hantaan and Seoul virus in the genus Hantavirus, family Bunyaviridae. In order to elucidate the role of maternal immunity to Hantavirus infection in rats, the protective effect of the maternal antibody were studies by using rats experimentally infected with Seoul virus strain HR80-39. Antibody titers of sera and viral antigen against Seoul virus were investigated by indirect immunofluorscence antibody technique (IFA). The dam sera had IFA antibody titers ranging from 1:128 to 1:1,024 after parturition. In fetuses, IFA antibody titers ranged from 1:16 to 1:64 just after birth, increased to peak titers ranged from 1:256 to 1:1,024 in the 2nd week after birth. Challenged newborn rats had IFA antibody titers ranging from 1:64 to 1:1,024 after inoculation. No viral antigen was detected in lungs or other organs of the newborn rats. The maternal antibody to Seoul virus was transferred prenatally through placenta and postnatally via colostrum from immune dams to their offspring. These results demonstrated that maternal antibody to Seoul virus was quite effective in protecting newborn rats against same virus infection.

자연보호의 개념과 가치에 관한 연구

우형택 대구효성가톨릭대학교 1998 연구논문집 Vol.58 No.2

This study was conducted to find out the new meaning and role of nature conservation in pursuing sustainable development and to provide theoretical bases for values of nature conservation from diverse academic perspectives. Nature conservation and sustainable development have the same purpose of balancing environment with development. Similarly, the two principles of nature conservation, sustainable yield and multiple use, and those of sustainable development, equity and limitation, call for equitable and sustainable use of natural resources by everyone within present generation and between generations. But sustainable development is more comprehensive concept requiring not only environmental sustainability but also changes in our political, economic, social, and technological systems. It is, therefore, concluded that nature conservation is a core part of sustainable development and the pursuit of sustainable development requires nature conservation. The new meaning of nature conservation can be derived from its significant role to be played in realizing sustainable development. Nature conservation has diverse and high values including: ethical values; aesthetic and recreational values; scientific and cultural values; economic and material values; ecological and life-supporting values; and social just and democratic values. It is finally suggested that the effective and strong nature conservation can be pursued and achieved in close connection with social justice and democratic practice in addition to other values of nature conservation.

신증후출혈열 환자의 혈청학적 및 분자생물학적 진단 검사법 비교

우영대,문희주,배형준 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.2

우리나라에서 발생하고 있는 급성 출혈성 질환인 신증후출혈열의 원인 바이러스는 Family Bunyaviridae의 Genus Hantavirus에 속하는 한탄과 서울바이러스에 의하여 발생되고 있다. 본연구에서는 신증후출혈열로 의뢰된 환자에서 한탄바이러스에 대한 항체가를 간접면역형광항체법(indirect immunofluorescent antibody technique, IFAT), 면역효소측정법(enzyme-linked immunosorbent assay, ELISA) (IgG, IgM), 고비중입자응집반응(high density composite particle agglutination, HDPA) 및 플라크감소중화시험(plaque reduction neutrafization test, PRNT)등으로 비교 측정하였고, 신증후출혈열환자로 확진된 15명의 한타바이러스 혈청형을 PRNT와 혈청형 특이 역전사 효소 중합효소연쇄반응(nested reverse transcriptase polymerase chain reaction, nested RT-PCR)으로 확인하였다. 신증후출혈열로 의뢰된 환자에서의 한탄바이러스에 대한 IFAT, ELISA(IgG,IgM),HDPA 그리고 PRNT 비교에서 형광항체, ELISA IgG, 응집항체 및 중화항체는 8명 모두 높게 나타났으며, ELISA IgM은 5명에서는 현저히 높은 항체를 보유하고 있었다. 신증후출혈열 환자 15명에서는 높은 형광항체와 중화항체 역가를 나타내었고, 15명 중 12명은 한탄바이러스, 2명은 서울바이러스에 대한 높은 중화항체를 갖고 있었으며, 1명은 두 바이러스에 대하여 동일한 항체 역가를 나타내었으며, 혈청형 특이 primer를 사용한 nested RT-PCR에서는 15명 중 3명과 1명만이 한탄바이러스와 서울바이러스 primer에 대해 RNA가 검출되었다. The etiologic agents of haemorrhagic fever with renal syndrome(HFRS) in Korea are Hantaan and Seoul virus in the genus Hantavirus, family Bunyaviridae. Antibody titers of sera from HFRS patients against Hantaan virus were measured by immunofluorescent antibody technique(IFAT), enzyme-linked immunosorbent assay (ELISA), high density composite particle agglutination (HDPA) and plaque reduction neutralization test (PRNT). PRNT and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) was used for serotypic differentiation of Hantaviruses against Hantaan and Seoul virus. Eight doubtful HFRS patients showed higher fluorescent, IgG ELISA, agglutination and neutralizing antibody titer by IFAT, ELISA IgG, HDPA and PRNT, respectively. Five out of them showed high IgM antibody titer by IgM capture ELISA against Hantaan virus, remarkably. Fifteen HFRS patients showed higher fluorescent antibody titer by IFAT. In PRNT, 12 out of them showed high neutralizing antibody titer aginst HTNV, 2 against SEOV and 1 against both viruses. In nested RT-PCR using serotype specific-primer, 3 out of them showed positive against HTNV and 1 against SEOV.

함침액막에서 운반체의 구조에 따른 트립토판의 플럭스

우인성,신석재,안형환,강안수 明知大學校 産業技術硏究所 1991 産業技術硏究所論文集 Vol.10 No.-

The relations between membrane potential and the flux of solutions, which were developed from the molecular structure of quaternary ammonium salts as carrier, have been analyzed for the counter transport system of tryptophan and nitrate anion across the supported liquid membrane. The liquid membrane was prepared by dissolving quaternary ammonium nitrates in toluene. The experimental results showed that the flux of tryptophan increased with increasing the carbon chain length of the alkyl group of carrier in the range of ??∼??, but in case of TDDAN, it decreased because of large membrane potential. The flux of tryptophan with the membrane structure of carrier increased in the order : Aliquat 336N, TOAN, TDDAN, and TDAN.

기도저항 변화가 설골근 활동성에 미치는 영향

우종윤,이상호,송형근 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1

There are some morphological changes in chronic mouth breathers, for example, a clock-wise rotation of mandible, extended posture of head, a lowered position of the tongue and the hyoid bone. When the intraluminal airway pressure becomes negative by the increase in airway resistance during inspiration, the airway can be collapsed. It was reported that hyoid position has a key role for maintaining airway patency. Anterior movement of hyoid bone can widen the airway and thus prevent airway obstruction. Many hyoid muscles attached to hyoid bone might control the position of hyoid. The present study was, therefore, designed to investigate the changes of activity of some hyoid muscles and the control mechanisms during increase in airway resistance. We obstructed the trachea or applied the sustained negative pressures to the isolated upper airway in 10 spotaneously breathing anesthetized dogs. The peak amplitudes of rectified and integrated eletromyogram(EMG)s from the geniohyoid, sternohyoid and sternothyroid were recorded. 1. As airway resistance increased, airway pressure were decreased and the peak amplitudes of integrated EMGs from those muscle were increased. Activities of all 3 muscles were increased during the first occluded breathing when the airway was occluded in the end of expiration. 2. The peak amplitudes of EMGs from those muscles were increased during applications of the negative pressure in the isolated upper air way. We could find these changes in the next breathing from pressure application. These results indicate that the negative pressure generated in upper airway augments the activities of hyoid muscles through the mechano-receptor activation and so does the deflation of lung. We speculate that geniohyoid and infrahyoid muscles cooperate for the anterior and inferior movement of hyoid bone.

수술방법에 따른 액취증의 합병증 비교

김형우, 이의태 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적 : 액취증은 겨드랑이에서 암내라고 불리는 특징적인 냄새를 내는 증상으로 비교적 흔하면서 환자에게 사회적, 정신적으로 많은 괴로움을 주는 질환이다. 이러한 액취증을 치료하기 위해 과거로부 터 수술적 절제술이 많이 시술되어 왔다. 하지만 불행하게도 수술적 절제술은 여러 가지 합병증이 보고 되고 있다. 현재, 액취증을 치료하기 위해 흡입술이 도입되면서 기존 치료방법과 효과와 만족도에 대한 비교의 필요성이 대두되어 왔다. 대상 및 방법 : 2005년 7월부터 2009년 3월까지 충북대학교병원 성형외과에서 액취증 일차 수술을 한 환자는 총 24명(남자 15, 여자 9)으로 이중 수술적 절제술이 8명, 흡입술이 16명이었다. 추적은 의무기록 검토 및 전화통화를 통한 설문으로 이루어 졌으며, 시술을 받은 24명의 환자중 수술적 절제 술 8명 중 7명(87.5%), 흡입술 16명 중 10명(62.5%)과 인터뷰가 이루어 졌다. 나머지 환자들은 수 신거부, 전화번호변경 등으로 추적하지 못했다. 인터뷰가 이루어진 17명의 환자들은 최소 10개월(평균 27.3개월) 간의 추적기간을 거쳤다. 액취제거에 대한 각 시술의 효과는 매우좋음, 좋음, 보통, 나쁨, 시술에 대한 만족도는 매우만족, 만족, 보통, 불만족 이렇게 각각 4단계로 분류하였다. 결과 : 효과에 대해서 수술적 절제술을 시행한 환자군에서는 매우좋음 4명(57.1%), 좋음 3명(42.9%) 으로 모두 좋음 이상의 답변을 하였고 흡입술을 시행한 환자군에서는 매우좋음 1명(10%), 좋음 5명 (50%), 나쁨 4명(40%)으로 답변을 하였다. 만족도에 대해서 수술적 절제술을 시행한 환자군 중 5명 (71.4%)이 만족 이상을 답변하였고, 흡입술을 시행한 환자군 중 4명(40%)이 만족 이상을 답변하였다. 합병증으로 수술적 절제술의 경우 과색소침착을 동반한 경계부위 조직괴사 및 과색소침착, 상처개열, 혈 종 각 1명으로 나타났고(총 3/7, 42.9%), 흡입술에서는 어떠한 합병증도 발견되지 않았다(0%). 결론 : 수술적 절제술 환자의 효과 및 만족도가 더 높지만 합병증이 흔한 것으로 나타났으며 흡입술의 경우 합병증은 없지만 효과 및 만족도가 떨어지는 것으로 나타났다. 이에 따라 두가지 방법 어느 하나 가 다른 하나에 비해 반드시 우월하다고 볼 수는 없는 것으로 보이며 환자와 상황에 따라서 알맞은 방 법을 쓰는 것이 좋을 것으로 생각된다.

일회 호흡법(Single breath method)을 사용한 심박출량 측정

우성,조형상 중앙대부설한국의과학연구소 1984 한국의과학 Vol.16 No.4