蜜蜂의 濃度가 人蔘正果의 品質에 미치는 影響

朱鉉圭 건국대학교 1985 建農學報 Vol.11 No.1

複合微生物을 利用한 된장開發에 關한 硏究

朱鉉圭 建國大學校附設 農業資源開發硏究所 1978 論文集 Vol.4 No.-

韓國 고유의 된장을 開發하기 위하여 黃麴菌, 黑麴菌 및 Rhizopus delemar 와 Bacillus natto로 Koji를 각각 만들고, 코오지의 配合比를 달리하여 된장을 담그어 熟成過程中에 化學成分의 調査와 관능검사를 실시한 結果는 다음과 같다. 1. 複合微生物을 이용한 된장의 아미노태질소는 一般된장의 규격보다 현저히 많았고, 黃麴+黑麴, 黃麴+黑麴+Rh.D 코오지(B) B+Natto를 混合한 것은 對照區와 同一하게 서서히 增加되었으나 黃麴+Rh.D 코오지 또는 黃麴+Rh.D 麴+natto를 混合한 된장은 對照區보다 急增되다가 23日후부터 減量되었다. 2. 各處理區의 환원당량은 숙성 23日에 對照區보다 많았고 그중 Natto를 첨가한 된장과 黑麴+Rh.D. 코오지만으로 담근 된장에서 제일 많았다. 3. 處理區中의 總酸의 含量은 對照區보다 현저하게 많았고 그중 黑麴이 混合된 된장에서 더 많았다. 4. 관능검사에서 各處理區는 對照區와 같거나 그보다 良好하였다. 그중 黃麴에 黑麴을 配合한 것보다 Rhizopus delemar麴을 混合한 된장, 또는 여기에 Natto와 黑麴을 混用한 된장이 더 良好하였다. In order to develop Korean traditional "Denjang" by using microorganism, several kinds of Denjang were made by mixing different rate of various Kojis such as Asp. oryzae, Aspniger, Rhizopus delemer(Rh.D)and Bacillus natto, and the quality of Denjangs was examined by panel discussion or chemical component analysis. The results obtained were as follows. 1. The "Denjang" produced by using the composite microorganism turn out higher NH₂-N content than standard commercial Denjang. The products of Denjang by using Asp. Oryzae Koji+Asp. niger Koji(A), Asp. oryzae Koji+1/2 Asp. niger Koji+1/2Rhi, D. Koji (B) and (B)+Natto showed gradual increase of NH₂-N content, however that added Asp. oryzae Koji+Rh. D. koji or Asp. oryzae Koji+Natto showed rapid increase of NH₂-N content in early stage and NH₂-N content gradually decreased after 23 days. 2. The above mentioned products showed higher content of reducing sugar than that control which was produced with Asp. oryzae only at after 23days fermentation among the treatments, Asp. oryzae Koji+Natto and Asp. niger Koji+Rhi, D. Koji showed the highest acid contents of reducing sugar. 3. Total acid content in Denjangs produced by using composite microorganism was extremely higher than that of the control and the Denjang added Asp. niger Koji showed the highest acid content among the all treatments. 4. In panel discussion, the Denjangs made by mixing Asp. oryzae Koji+Rhi, D. Koji (C) or (C)+Natto were judged of better quality than that the one added Asp. oryzae Koji+Asp. niger Koji only.

人蔘엑기스가 흰쥐의 發育에 미치는 影響

朱鉉圭 建國大學校附設 農業資源開發硏究所 1976 論文集 Vol.2 No.-

흰쥐 60마리를 표준군(C), 인삼첨가군(G), 인삼과 비타민첨가군(GV), 비타민 첨가군(V)으로 나누어 60일간 사육하면서 흰쥐의 成長率, 臟器重量 및 血液成分의 變化를 調査한 結果는 다음과 같다. 1. 體重增加率은 표준군에 比하여 人蔘첨가군이 약간 높은 增加率을 보였고 비타민과 인삼의 복합투여군은 유의성있는 차이는 아니었다. 2. 各處理群의 臟器重量을 比較하면 심장과 간, 폐에서는 표준군보다 人蔘添加群이 낮은 重量을 보였다. 3. 血液中의 血糖量은 표준군보다 人蔘群 및 人蔘과 비타민첨가군이 낮은 含量을 나타내었다. This study was performed to investigate the effects of Ginseng extract on the growth of rats. Ginseng, Vitamin and both were given to 60 rats for 2 months. The results were obtained as follows; 1. The body weight showed some what high rate in Ginseng group as compared with the body weight in control group. 2. The weight of the intestines, including the heart, liver and lung, in Ginseng group was less than in control group 3.Ginseng and Ginseng plus vitamin group had less blood sugar content than control group.

醱酵왕겨飼料가 병아리의 發育에 미치는 影響

朱鉉圭 建國大學校附設 農業資源開發硏究所 1977 論文集 Vol.3 No.-

韓國特許 第275號에 依하여 製造한 발효왕겨사료에 關한 飼養價식實驗은 다음과 같다. 各試驗群은 育雛用飼料에 발효왕겨사료를 配合한 群(5%, 10%, 30%, 50%)과 밀기울대신 代替한 群(3%, 5%, 10%, 20%)으로 나누어 初生雛(한협603) 480首에 8週間 飼育하고 增體量, 飼料攝取量, 飼料要求率, 生存率, 經濟性 等을 調査한 結果는 다음과 같다. ① 왕겨발효사료의 配合 및 代替量이 減少되고 飼料攝取量은 增加하였다. ② 밀기울의 3%, 5%, 10% 代替區는 對照區보다 增體量이 많았다. ③ 飼料攝取量은 시험사료配合群보다 밀기울 代替群이 많았다. ④ 飼料要求率은 3%(T₁), 5%(T₂)區가 對照區보다 良好하였고 그 外의 處理區는 왕겨발효사료의 增加順으로 많았다. ⑤ 生存率은 99.74%로 시험사료에서도 無關하였다. ⑥ 經濟性으로보아 3∼5%의 밀기울 代替群은 對照區보다 組所得 19.2∼19.8%가 增加하였다. For the feeding experiments of fermented rice-hull feeds prepared by Korean patent No.275,the feeding group were divided into two group of the standard and treating group. The feeds of standard group were prepared by mixing the fermented rice-hull of 5%(s₁),10%(s₂), 20%(s₃),30%(s₄), and 50%(??)to the chicken fodders made by Kon-kuk feeds Co., The feeds of the treating group were prepared by mixing the rice-hull of 3%(T₁),5%(T₂), 10%(T₃) and 20%(T₄) insted of wheat bran to the same fodders. The both feeds were supplied to 480 heads for 8 weaks and the following resulte were obtained. 1. When the mixed ratio of fermented rice-hull and the wheat bran were increased, the ate fodder amount were increased but the body-weight were decreased. 2. When they were bed by the treating group of 3%(T₁),5%(T₂) and 10%(T₃)the body-weight and growth-rate were more increased than control group. 3. The amount of feed intake in treated group were larger than the control group. 4. The feeding efficiency of the treated groups(T₁and T₂)were much better than the control group and the efficiency of the other group were increased in the order of mixed ratio of mixed ratio of fermented rice-hull. 5. The Viability were 99.74%. 6. Economically, 3%(T₁) and 5%(T₂)group showed low cost about 36.76-37.80 won than the control group.

複合微生物을 利用한 간장開發에 관한 硏究

朱鉉圭,李敎喆 建國大學校附設 農業資源開發硏究所 1980 論文集 Vol.5 No.-

韓國 고유의 간장을 開發코자 Aspergillus oryzae, Aspergillus niger 및 Rhizopus delemar와 Bacillus natto 菌으로 製麴하고 kogi의 配合比를 달리하여 간장을 담구고 熟成(7週) 過程 중에 化學成分의 調査와 관능검사를 실시한 結果는 다음과 같다. 1) 간장시료 중의 아미노태 질소함량은 혼합미생물시험구(0.57∼0.79%)가 Asp.oryzae만의 대조구(0.48%)보다 많았고 그중 Asp. oryzae+Rhiz. delemar 시험구(0.79%)가 가장 많았으며 Asp. niger의 혼합은 Rhiz. delemar보다 아미노태 질소의 유리량이 적었다. 2) 혼합미생물처리구(4.37∼7.96%)의 환원당량은 대조구(3.53%)보다 많았으며 그중 Asp. niger+Rhiz. delemar시험구가 제일 많았다. Asp. oryzae에 Rhiz. delemar의 복합은 Asp.niger보다 환원당량이 많았다. 3) 혼합미생물처리구의 총산량(0.38∼0.82%)은 대조구(0.33%)보다 많았고 그중 Asp. oryzae+Rhiz. delemar구(0.81%)와 Asp. oryzae+Rhiz. delemar+Bacillus nato구(0.82%)가 제일 많았으며 Asp. oryzae+Rhiz. delemar구는 Asp. oryzae+Asp. niger구보다 더 生酸量이 많았다. 4) 관능검사의 총점수에서 대조구(25점)보다 많은 시험구는 3구인데 그중 Asp. oryzae+Rhiz. delemar+Bacillus nato구(30점)가 가장 良好하고 다음 Asp. oryzae+Asp. niger, Asp. oryzae+Asp. niger+Rhiz. delemar구(29점)이다. In order to develop better soy-sauce, the research made different kinds of Koji, using microorganism such as Aspergillus oryzae, Aspergillus niger, Rhizopus delemar. and Bacillus natto each. Several kinds of soy-sauce, in which Kojis were mixed by different rate, were compared each other for their changes of chemical components during fermentation period (Seven weeks) and their quality examined by panel test. The results were summarized as follows; 1. The soy-sauce using the mixed koji, Asp. oryzae+Rhiz, delemar., showed the the highest NH₂-N content by 0.79% and the soy-sauce using the Koji made by composite microorganism showed higher NH₂-N content (0.57∼0.79%) than that of standard soy-sauce using Asp. oryzae Koji (0.48%). The content of NH₂-N in soy-sauce of Asp. oryzae+Asp. niger Koji was less than in soy-sauce of Asp. oryzae+Rhiz. delemar Koji. 2. The content of reducing sugar in soy-sauce was highest when the mixed Koji, Asp. niger+Rhiz, delemar was used, the nest was the composite microorganism Koji by 4.37∼7.96%, and the lowest was the standard commercial Koji by 3.53%. The mixed Koji using Asp. oryzae and Rhiz. delemar showed higher reducing sugar content than that of Asp. oryzae+Asp. niger Koji. 3. Total acid content in soy-sauce was highest when the mixed Kojis, Asp. oryzae+Rhiz. delemar and Asp. oryzae+Rhiz. delemar+Baillus natto., both by 0.81% and 0.82%, respectively. Total acid content in soy-sauce using composite microorganism Koji showed higher(0.38∼0.82%)than that of standard Koji(0.33%), and Asp. oryzae+Rhiz. delemar showed higher value than Asp. oryzae.+Asp.niger in total acidcontent. 4. Panel test score was highest for the soy-sauce used the mixed Koji, Asp. oryzae+Rhiz. delemar+Bacillus natto and the two kinds of soy-sauce used the mixed Koji, Asp. oryzae+Asp. niger and Asp. oryzae+Asp. niger+Rhiz, delemar., were judged as better than standard soy-sauce.

환자, 가축 및 하천에서 분리한 Escherichia coli에 대한 약제내성과 전달성 R-Plasmid에 관한 연구

정규선,이연태,김현주 숙명여자대학교 약학연구소 1997 약학논문집-숙명여자대학교 Vol.13 No.-

These studies were made to assess the present stage of resistance to antibiotics, incidence and transferability of R- factors against E. colL From March to July 1987, 59 strains of E. coli were isolated from specimens of patients collected at university hospitals in Seoul, 64 Strains from stools of domestic animals and 66 strains from drainages in Seoul. These specimens were tested for resistance to 12 kinds of antimicrobial agents by means of the agar dilution method. Using Miiller- Hinton agar for the assay of drug resistance and tryptic soy broth as propagating medium for conjugation. E. coli were found to be resistant to one or more antibiotics, and were considered to be potential donor of R- plasmid. 1. Drug resistant strains of E. coli isolated from patients, domestic animals and drainages were amounted to 55(93.2%), 33(51.6%) and 31(47.0%), respectively. 2. Resistance to APTc, and Cb was the highest in those isolated from patients and drainages, and resistance to Tc, Cm and Sm was the highest in those isolated from domestic animals. 3. In the transfer test of drug resistance by conjugation method, 17 strains(47.2%) isolated from patients, 15 ones(53.6%) isolated from domestic animals and 15 ones(55.6%) isolated from drainages showed positive results, transperable resistant plasmid molecules with variable in each strain.

캡슐화된 잠열 미립자 슬러리를 열 매체로 하는 축열식 냉방시스템

최준규,이재구,양현수,주태운,김필수 한국공업화학회 2003 응용화학 Vol.7 No.1

Microcapsules containing tetradecane as the phase change material (PCM) and ashell of meolamine-formalin resin were prepared by in situ polymerization. Tooptimize the encapsulation process, experiments were performed under various reaction conditions. Microcapsules with a high uniformity could be prepared, and the ratio of breakage of the microcapsules was less than 6% over 10,000 cycles using by a circulation pump. The cooling system, which has adopted MPCM slurry as a media for transporting cold thermal energy, is designed to investigate the performance of newly developed coolant. The discharging times of cold energy in circulating 20 wt% MPCM slurry are lasted to 260 minutes as long as the sensible cooling system. According, it was demonstrated that the microcapsules prepared in the current research are suitable for potential application as hat transporting media.

경주두 도달법 및 척골 신경 전방 전위술을 통한 상완골 원위부 과간 골절의 치료

이광현,이성필,황규태,김주학 대한골절학회 2004 대한골절학회지 Vol.17 No.2

Mycophenolic Acid가 PDGF에 의해 증식 유도된 흰쥐 혈관평활근 세포에서 NAD(P)H Oxidase, 세포내 활성산소족 및 Mitogen-activated Protein Kinases에 미치는 영향

박제현,하헌주,김명수,허규하,김유선 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Background: Vascular smooth muscle cell (VSMC) proliferation plays an important role in the development and progression of chronic allograft vasculopathy as in atherosclerosis. We already reported that mycophenolic acid (MPA) inhibited VSMC proliferation, cellular reactive oxygen species (ROS) and mitogen-activated protein kinases (MAPK) in human VSMCs. In this study, we examined further molecular mechanisms involved in the anti-proliferative effect of MPA in rat VSMCs. Methods: Primary rat VSMCs were stimulated with PDGF-BB 10 ng/mL in the presence or absence of MPA and various kinds of cell signaling inhibitors. Cell proliferation was assessed by [H³]-thymidine incorporation, NAD(P)H oxidase subunits mRNA expression by RT-PCR, dichlorofluorescein-sensitive cellular ROS by FACS, and the activation of PDGF receptor-β (Tyr 751), racl, and MAPK by Western blot analysis. Results: PDGF increased cell proliferation and cellular ROS, activation of PDGF receptor-β (Tyr 751), racl, expression of p22phox and MOX1 mRNA, ERK 1/2, and p38 MAPK, compared to control. MPA inhibited up-regulation of racl phosphorylation, p22phox and MOX1 mRNA expression, cellular ROS, and phosphorylation of ERK 1/2 and p38 MAPK. However, MPA did not affect PDGF receptor-β (Tyr 751) activation. Wortmannin, diphenyleniodonium (DPI), trolox, and NAC, each inhibited PDGF-induced ERK 1/2 and p38 MAPK activation. PD98059 and p38 MAPK inhibitor also inhibited PDGF-induced cell proliferation. Conclusion: These results suggest that MPA inhibits PDGF-induced VSMC proliferation through inhibiting NAD(P)H oxidase-dependent cellular ROS leading to ERK 1/2 and p38 MAPK activation. 배경 : 혈관평활근 세포의 증식은 장기이식 후 발생하는 혈관경화증이나 동맥경화증의 발생과 진행에 중요한 역할을 한다. Mycophenolic acid (MPA)는 강력한 면역억제제로서 혈관평활근 세포의 증식도 억제한다. 본 연구는 사람의 혈관평활근 세포에서 MPK가 세포증식에 관여하는 신호전달계인 활성산소족과 mitogen-activated protein kinases(MAPK) 활성화를 억제하는 기존의 연구결과를 바탕으로, PDGF에 의해 증식 유도된 흰쥐 혈관평활근 세포에서 MPA가 세포의 증식을 억제하는 기전을 검색하였다. 방법 : 일차 배양한 흰쥐 혈관평활근 세포를 PDGF-BB 10 ng/mL로 자극하였고, MPA를 비롯한 여러 종류의 신호전달 억제제는 PDGF를 투여하기 1시간 전에 투여하였다. 세포증식은 [H³]-thymidine incorporation으로, NAD(P)H oxidase subunit의 mRNA 표현은 RT-PCR로, dichlorofluorescein에 민감한 세포내 활성산소족은 FACS 방법으로, 그리고 PDGF 수용체-β (Tyr 751), racl 및 MARK 활성하는 Western blot으로 각각 분석하였다. 결과 : PDGF는 PDGF 수용체-β (Tyr 751)의 활성화 및 NAD(P)H oxidase subunit 중 racl의 활성화와 p22phox와 MOX1의 mRNA 표현을 대조군에 비하여 유의하게 증가시켰다. MPA는 PDGF 수용체의 활성화에는 영향을 주지 않았으나, racl의 활성화, p22phox와 MOX1의 mRNA 표현의 상향 조절, 세포내 활성산소족, 그리고 ERK 1/2와 p38 MAPK의 활의한 EFK 1/2와 p38 MAPK 활성화와 세포 증식을 억제하였다. PD98059와 p38 MAPK 억제제는 PDGF에 의한 혈관평활근 세포의 증식을 억제하였다. 결론 : 본 연구결과는 MPA가 NAD(P)H oxidase를 억제함으로써 세포내 활성산소족 생산과, MAPK 활성화를 억제함으로써 PDGF에 의하여 유도되는 혈관평활근 세포의 증식을 억제함을 시사하였다.

방울토마토중 Dichlofluanid 및 Iprodione의 생산단계별 잔류농약 경시변화

최규일,성기용,정태균,이주환,허장현,고광용,이규승 한국환경농학회 2002 한국환경농학회지 Vol.21 No.4

방울토마토의 생산단계에서의 잔류허용기준 설정과 수확후 저장기간중 경시변화 및 세척에 의한 잔류농약의 감소양상을 파악하였다. 해당약제를 안전사용기준의 기준량 및 배량을 각각 시설재배 포장에서 살포후 10일간 잔류양상을 조사하였고, 실온 및 냉장조건 하에서의 저장실험 및 세척에 따른 전류농약의 잔소량을 파악하여 생산단계부터, 출하, 저장, 소비단계까지의 잔류량을 예측할 수 있는 모델개발을 위한 자료로 이용하고자 연구를 수행하였다. Dichlofluanid와 iprodione은 포장조건에서 반감일수가 기준량 2.2, 3.3일 이었으며, 배량은 3.5, 5.4일로 나타났다. 저장조건하에서의 약제별 반감기는 포장조건보다 잔류량의 감소속도가 느린 것으로 나타나 반감일수가 증가하였고, 세척방법에 의한 잔류농약의 감소량을 조사한 결과 세제 사용시의 평균제거율은 dichlofluanid는 73.7%, iprodione은 64.3%로 나타났고, tap-water 사용시에는 dichlofluanid 73.5%, iprodione 63.5%로 나타났다. 이러한 결과는 작물의 생산단계에서 구한 합리적인 희귀식을 이용하여 잔류농약의 경시변화를 파악하고, 저장 및 세척과정을 거쳐 최종 소비단계에서의 잔류농약의 수준을 평가할 수 있었다. We studied the residual patterns of two fungicides, dichlofluanid and iprodione, in cherry tomato greenhouse after applying with the recommended and double dose. Also, the degradation patterns during storage periods of up to ten days were compared between at room temperature (20℃) and at cold temperature (4℃). Removal rates of fungicides by washing with tap-water and detergent solution (0.1%, 0.2%) were measured. Half-lives of dichlofluanid and iprodione in greenhouse cherrytomato were 2.2~3.5 and 3.3~5.4 days, respectively. During the storage period, the residues were dissipated more slow. Removal rates were 62.8~80.3% by tap-water, 60.4~83.1% by 0.1% detergent solution, and 65.3~77.6% by 0.2% detergent solution. So, we can predict of terminal residues from cultivation period to marketing, storage and consuming.