Prevalence of Pediatric Autism Spectrum Disorder in Korea Using National Statistics Including the COVID-19 Pandemic, 2011 to 2021

Hyeji Eom(Hyeji Eom),Minji Kim(Minji Kim),Jung Kyung Yoo(Jung Kyung Yoo),Eunjoo Bae(Eunjoo Bae),Jeonghyeon Yi(Jeonghyeon Yi),Ji Young Lee(Ji Young Lee) 대한소아신경학회 2022 대한소아신경학회지 Vol.31 No.1

Purpose: Regularly analyzing the prevalence of autism spectrum disorder (ASD) is important to inform policies and strategies for proper management. The present study aimed to esti-mate trends in ASD prevalence according to age and time in Korean children. Methods: We monitored the annual prevalence of ASD in children aged 2 to 18 years based on information from Statistics Korea and the Korean National Health Insurance Service. We estimated changes in prevalence between 2011 and 2021 after stratifying partici-pants into three age groups. Furthermore, we analyzed the prevalence of ASD by birth year. Results: The highest level of prevalence appeared in children aged 6 years, and thereafter, it de-clined with age. The prevalence of ASD in children aged 2 to 18 years increased from 0.05% in 2011 to 0.12% in 2021. During the same time, the prevalence in preschool children (2 to 5 years) remarkably increased from 0.04% in 2011 to 0.12% in 2021, while the prevalence in adolescents (13 to 18 years) increased from 0.05% in 2011 to 0.09% in 2021. The prevalence of ASD notably increased during the coronavirus disease 2019 (COVID-19) pandemic (2020 to 2021). Among children born between 2011 and 2015, the prevalence of ASD was higher for children with recent birth years, especially during the COVID-19 pandemic. Conclusion: The overall ASD prevalence in children and adolescents in Korea seems to be increasing, especially in preschool children during the COVID-19 pandemic. Early and more effec-tive interventions are necessary for Korean children.

Antimicrobial effect of phytochemicals to Listeria monocytogenes isolated from slaughterhouses

Hyeji Kim,Hyemin Oh,Heeyoung Lee,Sejeong Kim,Jimyeong Ha,Jeeyeon Lee,Yukyung Choi,Yewon Lee,Yujin Kim,Yohan Yoon,Soomin Lee 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.4

본 연구에서는 도축장에서 분리된 Listeria monocytogenes를 저해할 수 있는 식물성 항균 물질을 탐색하고, 각 항균 물질의 최소살균농도(MBC)를 확인하였다. 잠재적인 항균 물질로 자몽종자 추출물, 감귤류 추출물, 생강 추출물, 배 추출물, 매실 농축액, 도라지 추출물, 대추 추출물, 오미자 추출물을 선정하였고, 대상 균주로는 국내 도축장에서 분리한 15 개의 L. monocytogenes 균주를 혼합하여 사용하였다. MBC 확인 결과, 최대 4.0 μg/mL 농도의 생강 추출물, 배 추출물, 매실 농축액, 도라지 추출물, 대추 추 출물, 오미자 추출물은 고농도의 L. monocytogenes (7 Log CFU/mL)에 대해 균 저해 효과가 전혀 나타나지 않았다. 반면, 7 Log CFU/mL의 L. monocytogenes에 대한 자몽종자 추출물과 감귤류 추출물의 MBC는 0.002 μg/mL인 것으로 확인되었다. 3 Log CFU/mL의 L. monocytogenes에 대한 MBC를 시험해 본 결과, 자몽종자 추출물과 감귤류 추출물의 MBC는 0.001 μg/mL로 나타났다. 이상의 결과로 볼 때 자몽종자 추출물과 감귤류 추출물은 L. monocytogenes를 제어하기 위해 적용될 수 있을 것으로 판단된다. This objective of this study was to investigate inhibitory effect of natural antimicrobials on Listeria monocytogenes isolated from Korean slaughterhouses. A mixture of 15 strains of L. monocytogenes at low (3 Log CFU/mL)and high (7 Log CFU/mL) concentration was exposed to various extracts (grapefruit seed extract, citrus fruits extract, ginger extract, pear extract, Japanese apricot concentrate, balloon flower extract, jujube extract, and omija extract) at 0.001-4.0μg/mL . Ginger extract, pear extract, Japanese apricot concentrate, balloon flower extract, jujube extract, and omija extract showed no antimicrobial effects to high-concentration of L. monocytogenes (7 Log CFU/mL). However, grapefruit seed extract and citrus fruit extract showed antibacterial effects against L. monocytogenes at 3 and 7 Log CFU/mL with MBCs of 0.001 and 0.002 μg/mL, respectively. These results indicate that grapefruit seed extract and citrus fruits extract can be used to control L. monocytogenes as natural antimicrobials.

Oocyte maturation under a biophoton generator improves preimplantation development of pig embryos derived by parthenogenesis and somatic cell nuclear transfer

Lee, DJoohyeong,Shin, Hyeji,Lee, Wonyou,Lee, Seung Tae,Lee, Geun-Shik,Hyun, Sang-Hwan,Lee, Eunsong The Korean Society of Veterinary Science 2017 大韓獸醫學會誌 Vol.57 No.2

This study was conducted to determine the effects of biophoton treatment during in vitro maturation (IVM) and/or in vitro culture (IVC) on oocyte maturation and embryonic development in pigs. An apparatus capable of generating homogeneous biophoton energy emissions was placed in an incubator. Initially, immature pig oocytes were matured in the biophoton-equipped incubator in medium 199 supplemented with cysteine, epidermal growth factor, insulin, and gonadotrophic hormones for 22 h, after which they were matured in hormone-free medium for an additional 22 hr. Next, IVM oocytes were induced for parthenogenesis (PA) or provided as cytoplasts for somatic cell nuclear transfer (SCNT). Treatment of oocytes with biophoton energy during IVM did not improve cumulus cell expansion, nuclear maturation, intraoocyte glutathione content, or mitochondrial distribution of oocytes. However, biophoton-treated oocytes showed higher (p < 0.05) blastocyst formation after PA than that in untreated oocytes (50.7% vs. 42.7%). In an additional experiment, SCNT embryos produced from biophoton-treated oocytes showed a greater (p < 0.05) number of cells in blastocysts (52.6 vs. 43.9) than that in untreated oocytes. Taken together, our results demonstrate that biophoton treatment during IVM improves developmental competence of PA- and SCNT-derived embryos.

Effects of phytohemagglutinin and type of culture dishes on aggregation efficiency and developmental competence of parthenogenetic embryos in pigs

Joohyeong Lee,Ji Eun Park,Minji Kim,Hyeji Shin,Geun-Shik Lee,Seung Tae Lee,Eunsong Lee 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Poor embryo quality and low blastocyst formation have been major limitations in establishment of cloned embryonic stem cells and production of cloned animals through somatic cell nuclear transfer (SCNT). Aggregation of embryos is a promising method for improving developmental competence of blastocysts. The aim of this study was to improve the blastocyst formation and the quality of parthenogenetic (PA) pig embryos by the aggregation of blastomeres at the 4-cell stage that were cultured in various type of culture dishes with or without phytohemagglutinin (PHA). The PA embryos were produced by the general method of our laboratory. On Day 2 after PA, the zona pellucida of 4 cell-stage embryos were removed by treatment with 0.5% (wt/vol) pronase solution. The 3x zona-free blastomere (ZFB) were randomly distributed in each of the following treatments for aggregation. ZFB were cultured for 5 days at 39℃ in an atmosphere 5% CO2, 5% O2, and 90% N2. In Experiment 1, effect of culture dishes on the aggregation efficiency and developmental competence of PA embryos were investigated. ZFB were cultured on non-coated (control) culture dish or dishes coated with 1% (wt/vol) agarose substrate (AS) or Well of the Well in dishes coated with 1% (wt/vol) agarose substrate (WAS). The ZFB cultured in WAS showed significantly higher (P<0.05) aggregation (81.2%) than AS and control (21.6-45.5%). The mean cell number in blastocysts derived from AS and WAS (81.4-89.3 cells/blastocyst) was significantly higher (P<0.05) than that of control (63.8 cells/blastocyst). In Experiment 2, effects of 150 ug/ml PHA treatment on the aggregation efficiency and developmental competence of embryos were investigated. The ZFB cultured in AS with PHA showed a higher (P<0.05) aggregation rate (90.0%) than that in AS without PHA, control with PHA, and control (39.2%, 57.9% and 17.5%, respectively). In conclusion, aggregation of porcine ZFB treated with PHA and agarose substrate could be a useful technique for producing improving blastocyst development with increased mean cell number of blastocysts in pigs.

Antimicrobial Effect of Phytochemicals to Listeria monocytogenes Isolated from Slaughterhouses

Kim, Hyeji,Oh, Hyemin,Lee, Heeyoung,Kim, Sejeong,Ha, Jimyeong,Lee, Jeeyeon,Choi, Yukyung,Lee, Yewon,Kim, Yujin,Yoon, Yohan,Lee, Soomin The Korean Society of Food Hygiene and Safety 2018 한국식품위생안전성학회지 Vol.33 No.4

본 연구에서는 도축장에서 분리된 Listeria monocytogenes를 저해할 수 있는 식물성 항균 물질을 탐색하고, 각 항균 물질의 최소살균농도(MBC)를 확인하였다. 잠재적인 항균 물질로 자몽종자 추출물, 감귤류 추출물, 생강 추출물, 배 추출물, 매실 농축액, 도라지 추출물, 대추 추출물, 오미자 추출물을 선정하였고, 대상 균주로는 국내 도축장에서 분리한 15 개의 L. monocytogenes 균주를 혼합하여 사용하였다. MBC 확인 결과, 최대 $4.0{\mu}g/mL$ 농도의 생강 추출물, 배 추출물, 매실 농축액, 도라지 추출물, 대추 추출물, 오미자 추출물은 고농도의 L. monocytogenes (7 Log CFU/mL)에 대해 균 저해 효과가 전혀 나타나지 않았다. 반면, 7 Log CFU/mL의 L. monocytogenes에 대한 자몽종자 추출물과 감귤류 추출물의 MBC는 $0.002{\mu}g/mL$인 것으로 확인되었다. 3 Log CFU/mL의 L. monocytogenes에 대한 MBC를 시험해 본 결과, 자몽종자 추출물과 감귤류 추출물의 MBC는 $0.001{\mu}g/mL$로 나타났다. 이상의 결과로 볼 때 자몽종자 추출물과 감귤류 추출물은 L. monocytogenes를 제어하기 위해 적용될 수 있을 것으로 판단된다. The objective of this study was to investigate the inhibitory effect of natural antimicrobials on Listeria monocytogenes isolated from Korean slaughterhouses. A mixture of 15 strains of L. monocytogenes at low (3 Log CFU/mL) or high (7 Log CFU/mL) concentration was exposed to various extracts (grapefruit seed extract, citrus fruit extract, ginger extract, pear extract, Japanese apricot concentrate, balloon flower extract, jujube extract, and omija extract) at $0.001-4.0{\mu}g/mL$. Ginger extract, pear extract, Japanese apricot concentrate, balloon flower extract, jujube extract, or omija extract showed no antimicrobial effects on high-concentration of L. monocytogenes (7 Log CFU/mL). However, grapefruit seed extract and citrus fruit extract showed antibacterial effects against L. monocytogenes at 3 and 7 Log CFU/mL with MBCs of 0.001 and $0.002{\mu}g/mL$, respectively. These results indicate that grapefruit seed extract and citrus fruits extract can be used to control L. monocytogenes as natural antimicrobials.

Effect of Insulin and Epidermal Growth Factor Treatment During In Vitro Growth Culture on Growth and Development of Pig Oocytes Derived From Small Antral Follicles

Hyeji Shin,Minji Kim,Ji Eun Park,Joohyeong Lee,Seung Tae Lee,Eunsong Lee 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

This study was conducted to evaluate the effects of insulin and epidermal growth factor (EGF) in a in vitro growth (IVG) medium on oocyte growth, in vitro maturation (IVM) and embryonic development of pig oocytes derived from small antral follicles (SAF) less than 3 mm in diameter. SAF oocytes were cultured for 2 days to induce IVG in alpha-minimal essential medium supplemented with 1 mM dbcAMP and 15% (v/v) fetal bovine serum. After IVG culture, oocyte maturation was induced by culturing IVG oocytes in IVM medium for 44 h. IVM oocytes that extruded the first polar body were selected and induced for parthenogenesis (PA) by applying electric stimulus. SAF oocytes cultured under the insulin treatment showed a significantly increased (P < 0.05) nuclear maturation (73.8%) compared to those cultured with insulin and EGF (59.8%). After PA, the proportions of blastocysts based on the number of metaphase II oocytes were significantly higher (P < 0.05) in oocytes that were cultured for IVG with insulin, EGF, and insulin + EGF (32.4%, 35.2%, and 34.8%, respectively) than in control (22.9%). IVG oocytes treated with insulin showed an increased oocyte diameter (116.3 μm) compared to those treated with insulin and EGF (114.0 μm) (P < 0.05). Intra-oocyte GSH content significantly increased (1.07 pixels/oocyte) by insulin treatment during IVG compared to that of oocytes treated with insulin + EGF (0.78 pixels/oocyte). These results demonstrate that IVG culture of SAF oocytes under insulin or/and EGF treatment supports oocyte maturation and improves embryonic development to the blastocyst stage after PA in pigs.

Isolation and Characterization of Probiotic Bacillus subtilis 1-1 Possessing L-Asparaginase Activity from Soybean Fermented Food

Hyeji Lim,Jina Choi,Huiyeong Seong,Hyunah Jang,Jiwon Yoon,Min-Ji Kim,Soo-Jeong Lee,Sungryul Yu,Youngseung Lee,Yoonhwa Jeong,Misook Kim 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

Toxic Leukoencephalopathy by Accidental Oral Ingestion of an Infant’s Fentanyl Patch

Hyeji Lim,Hyunjoo Lee,Young-Mock Lee 대한소아신경학회 2021 대한소아신경학회지 Vol.29 No.2

Collision Avoidance based on CPA algorithm using Relative Distance and Azimuth of Radar for Unmanned Aerial Vehicles

Hyeji Kim,Cheonman Park,Seongbong Lee,Dongjin Lee 제어로봇시스템학회 2021 제어로봇시스템학회 국제학술대회 논문집 Vol.2021 No.10

In this paper, we propose an obstacle states estimation algorithm based on radar and a collision avoidance algorithm based on closest point of approach(CPA) for unmanned aerial vehicles(UAVs), in order to avoid collision with the obstacle that do not provide information. We estimated position of detected objects using radar and compared current position and previous position for matching objects and removing outliers. Among the detected objects, the obstacle was selected the object with the highest risk of collision. The CPA was calculated using estimated states of the UAV and the obstacle. Based on the CPA, we detected collision and generated the velocity command for collision avoidance. The performance of proposed algorithm of states estimation of obstacle, collision detection and avoidance was verified through the flight tests.

Schizandrin Prevents Cytoplasmic TDP-43 Accumulation in Neuronal Cells

Hyeji LEE,Jisun OH,Nam Keun LEE,Yong-Seob JEONG 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.10