연작업자들의 신기능 평가에서 요중 δ-aminolevulinic acid 측정의 의의

이병국,이숙,김용배,리갑수,김화성,황규윤,장봉기,이성수,안규동 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

To clarify the effect of occupational lead exposure on renal function 241 lead workers working and 56 non lead workers were studied in 5 lead acid battery industries. Study variables for renal effect were urinary N-acetyl-β-D-glucosaminidase(NAG), urinary total protein(U-TP), blood urea nitrogen(BUN), serum creatinine(S-Cr), and serum uric acid(S-Ua). On the other hand, blood lead(PbB), zinc protoporphyrin in whole blood(ZPP), and delta-aminolevulinic acid in urine(δ-ALA) were selected for the variables of lead exposure. Information on age, work duration, smoking and drinking history were also obtained. The results obtained were as follows. While the mean values of lead exposure indicators of lead workers were significantly different from non-exposed ones, the mean values of BUN, U-TP and S-Cr of renal function indicators of exposed were also different from non-exposed. BUN, U-TP and NAG showed statistically significant correlation with PbB and ZPP, and NAG revealed also statistically strongly significant correlation with δ-ALA. On multiple regression using renal fuction indicators as a dependent variable and δ-ALA, age, work duration,lead exposure,smoking and drinking as independent variables, only δ-ALA contributed to NAG.

전자부품 제조업 근로자들의 시력변화에 대한 연구

이용만,안규동,이성수,함정오,김화성,리갑수,이병국 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

근접작업이 시력약화의 원인이 될 수 있는가를 알아보기 위하여 전자부품을 제조하는 2개 사업장에서 1991년에서 1993년까지 입사한 근로자들 중에서 근접작업으로 인정되는 마이크로칩을 조립하는 근접작업자 306명을 조사군으로, 대조군은 세탁기 타이머 및 일반 기계기구를 조립하는 작업자 154명, 계 460명을 선정하였다. 이들 대상자는 1991년 입사자는 1993, 1994, 1995년 시력을 조사하였으며, 1992년 입사자는 1993, 1994, 1995년 3년동안 조사를 하였으며, 1993년 입사자는 1994, 1995년 2년간 시력을 조사하였다. 대상자 중에서 입사시 안경을 착용한 근로자는 82명(17.8%)이었다. 따라서 남자 근로자와 안경착용자를 제외한 여성 근로자들 335명만을 추적 조사하여 다음과 같은 결론을 얻었다. 조사군에서 입사시 시력 1.0 이상이었던 근로자의 분포는 취업 1년 후 약 25-35% 정도 감소하였으며, 2년후에는 약 40-60% 정도의 감소를 나타내었고, 3년 후 약 50-65% 정도로 감소하였고 4년후에는 약 65% 이상 감소하였다. 그러나 대조군에서는 1.0 이상의 시력을 보였던 근로자들의 비율이 취업 1년 또는 2년 후 15-30%의 감소를 보이고 있으나 그 이후에는 큰 변동이 없었다. 이들의 시력변화를 근무년수에 따라 알아보기 위하여 조사된 시력을 순위변수로 하여 Kruskall-Wallis 검정을 시행한 결과 조사군, 대조군 모두에서 근속년수에 따른 순위의 합의 평균이 년차적으로 유의하게 감소하였으나, 입사시부터 근속년수에 따른 조사군과 대조군과의 순위합의 평균에 의한 시력차 비교에서 입사시 조사군의 순위합의 평균이 유의하게 높았던 것이 근무기간에 따라 대조군의 평균이 높아지면서 통계적 유의성이 없고 대체적으로 1년 이후부터 변화가 적은 것으로 나타나 근접작업으로 인한 시력약화를 추정할 수 있었다. This study was carried out to investigate that the close distance works were associated with decreased visual acuity. 306 workers were subjects who had been recruited from 1991 to 1993 in two electronic parts manufacturing factories, and 154 workers were controls who had been employed from 1991 to 1993 in launder machine timer and general machine assembly factories. Subjects who had been employed in 1991 and 1992 were follow up from 1993 to 1995, and subjects who had been employed in 1993 were from 1994 to 1995. The male workers as well as glasses wearing workers were excluded. Only 335 female workers were followed up to study the change of visual acuity. The results were as follows; The rate of workers with visual acuity over 1.0 at recruit year in subjects group was decreased about 25-35% after 1 year, 40-60% after 2 years, 50-65% after 3 years, and 65% after 4 years. While, the rate of workers with visual acuity over 1.0 at recruit year in control group was decreased about 15-30% after 1 or 2 years, and there was no more additional visual acuity reduction after then. Kruskall-Wallis test was performed to investigate the change of visual acuity in subject and control group by work duration. The means scores of ranks in both group were significantly decreased by work duration. The mean scores of ranks in subject group at recruit year were significantly higher than those in control group at recruit year, but these differences were disappeared after 1 year work. Therefore, this suggest that close distance workers may be affected their visual acuity.

납 폭로 근로자에서 δ-aminolevulinic acid dehydratase 유전형질이 zinc protoporphyrin 및 δ-aminolevulinic acid에 미치는 영향

이병국,황규윤,장봉기,김용배,리갑수,안현철,김화성,이성수,안규동,박종범 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

The inhibition of δ-aminolevulinic acid dehydratase (ALAD) in the heme synthetic pathway results in increased protophoryphyrin (ZPP) and δ-aminolevulinic acid (ALA) and is responsible for some of toxicological effects of lead. This enzyme is coded by the ALAD gene containing 2 co-dominant alleles. The polymorphisms of ALAD gene may be related to differential effects of lead on ZPP and ALA, ALAD genotype was measured in 975 Korean male lead workers, of whom 897 were homozygous for ALAD1 (ALAD 1-1 genotype) and 96 were heterozygous for ALAD2 (ALAD 1-2 genotype). Blood lead in subjects with ALAD1 was significantly higher than those with ALAD2 (p = 0.01). No difference between ALAD genotypes was found for age, exposure duration, ZPP, ALA, hemoglobin, hematocrit, body mass index, tobacco and alcohol use. After adjustment for possible confounders, ALA and ZPP became significantly elevated in ALAD1 subjects (p = 0.004 and 0.055, respectively). This result suggests that ALAD1 subjects may be more susceptible to the hematotoxicologic effects of lead than ALAD2 subjects.

납 노출자에 ALAD 및 VDR 유전자의 다형성이 혈중 납과 혈압과의 관련성에 미치는 영향

리갑수,황보영,김용배,김화성,이성수,안규동,이병국 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

To evaluate the effect of ALAD and VDR polymorphism on the relationship of blood lead with blood pressure in lead exposed workers, 801 lead exposed workers were selected. Blood lead was selected as parameters of lead dose and systolic and diastolic blood pressure using Random Zero Sphygmomanometer were chosen as blood pressure. ALAD and VDR genotype of each subject was assayed. Demographic information such as sex, age, education, past and family history, and personal habit of smoking and drinking were collected. Job duration of lead exposed workers was also obtained. The means of age and work duration of the subjects were 40.4±10.1 years and 8.2±6.5 years. The means of systolic blood pressure and diastolic blood pressure of the subjects were 123.2±16.3 mmHg and 75.8±11.9 mmHg. The overall prevalence of hypertension was 9.7%. ALAD2, the variant allele, in lead exposed workers was 10% and ALAD1 was 90%. For VDR genotype, the Bb or BB genotypes were 11.2%, while the bb genotype was 88.8%. The mean blood lead of subjects was 25.4±10.9 ㎍/㎗ (range 8.0-65.6). In the multiple regression analysis, blood lead was the significant positive predictor of systolic blood pressure and not the significant negative predictor of diastolic blood pressure after adjusting for possible confounders (age, sex, body mass index, use of anti-hypertensive drugs, drinking and smoking habits, VDR and ALAD genotypes. Also the subjects with the VDR Bx had 2.9 mmHg higher than those with the VDR bb (p=0.09). There was no interaction of VDR and ALAD genotypes between blood lead and systolic blood pressure. In multiple logistic regression analysis, the Odd's ratio of VDR genotype(BB or Bb vs. bb) for hypertension was 1.7 (95% confidence interval: 0.8-3.2). This study suggests that blood lead increases systolic blood pressure controlling covariates.

연폭로 근로자들에서 흡연 및 보건행태학적 변수가 주관적 자각증상에 미치는 영향

이성수,김화성,리갑수,안규동,이병국 순천향대학교 산업의학연구소 1997 순천향산업의학 Vol.3 No.1

To investigate the effect of smoking and drinking habit on the health status of lead workers, 220 male workers in lead using industries and 210 male controls were selected for this study. Selected study variables were lead in blood (PbB), zinc protoporphyrin in blood (ZPP), delta-aminolevulinic acid in urine(DALA), hemoglobin (Hb), and hematocrit (Hct). Questionnaire for lead related symptoms (15 symptoms) and smoking and drinking habit was provided to all the workers and filled up by themselves and reconfirmed by physician. The results obtained were as follows; 1. While smoking and drinking rate of lead exposed group were 77.7% and 75.8%, respectively, those of control group were 53.3% and 35.7%, respectively. 2. Mean values of lead exposed indices of lead workers was significantly different from other group. 3. In exposed workers, there was significant differences of DALA between smoker and non-smoker. 4. The neuromuscular symptom prevalence of lead exposure was higher in lead exposed group than non-exposed group. 5. Symptom prealence of lead exposure were higher in drinking and smoking group than non-drinking and non-smoking group. 6. While logistic analysis of positive neuromuscular symptom with categorized blood lead and age, smoking, drinking as categorized independent variables indicated that only blood lead (>40㎍/㎗) was significantly contributed to dependent variable with 95% significant odd ratio confidence interval, logistic analysis of positive constitutional symptom with same categorized independent variables indicated that only age (>40years) was significantly contributed to dependent variable. And logistic analysis of total symptom with same categorized independent variables indicated that blood lead (>40㎍/㎗) and age (>40years) were significantly contributed to dependent variable.

업종에 따른 연 취급 사업장의 기중 연 농도 및 연 노출 수준 평가

이병국,김용배,리갑수,안현철,김화성,이용진,황규윤,장봉기,이성수,안규동 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

In order to obtain an useful information for health management and biological monitoring of lead exposed workers, Authors tried to investigate air lead level and the lead exposed level of workers in lead industry according to occupational category. The subjects in our study were 2074 workers in 7 lead-using industries, and study subjects were divided into 4 occupational categories such as storage battery industry (type 1), primary smelting industry (type 2), secondary smelting industry (type 3) and litharge making industry (type 4). Blood zincprotoporphyrin concentration (ZPP), blood lead concentration (PbB) and urinary δ -aminolevulinic acid (ALAU), hemoglobin (Hb), hematocrit (Hct) were selected as the indices of lead exposure. Personal variables such as age, work duration were also collected. The results were as follows. 1. The geometric mean air lead in 9 lead-using industry was 0.1133±4.3120 ㎎/㎥, and that in type 1, 2, 3 and 4 was 0.1038±3.4952 ㎎/㎥, 0.0429±3.4329 ㎎/㎥, 0.1877±2.5123 ㎎/㎥ and 0.9961±5.2910 ㎎/㎥, respectively. 2. The mean ZPP in 9 lead-using industry was 53.1±28.0 ㎍/㎗, and that in type 1, 2, 3 and 4 was 52.0±24.8 ㎍/㎗, 48.0±16.4 ㎍/㎗, 109.8±85.6 ㎍/㎗ and 74.3±37.8 ㎍/㎗, respectively. There was significant difference in ZPP according to occupational category (P<0.01). The percents of lead workers whose ZPP were above 100 ㎍/㎗ in type 1, 2, 3 and 4 were 4.0%, 1.7%, 34.3% and 21.6%, respectively. 3. The mean PbB in lead-using industry was 26.0±11.2 ㎍/㎗, and that in type 1, 2, 3 and 4 was 25.3±11.1 ㎍/㎗, 26.7±8.8 ㎍/㎗, 50.3±15.2 ㎍/㎗ and 36.4±11.0 ㎍/㎗, respectively. There was significant difference in PbB according to occupational category (P<0.01). The percents of lead workers whose PbB were above 40 ㎍/㎗ in type 1, 2, 3 and 4 were 10.4%, 7.7% 71.9% and 43.2%, respectively. 4. While the correlation of Hb corrected PbB with ZPP was higher than non corrected PbB, the correlation of log-transformed ZPP with PbB was higher than non corrected ZPP. 5. Simple linear regressions of PbB and corrected PbB as independent variable with ZPP, log-transformed ZPP and ALAU as dependent variable were statistically significant (P0.01). Coefficient of determination of corrected PbB with other variables was higher than non corrected PbB. As the result of this study, it was suggested that reconsideration of environmental and biological monitoring program was highly recommended for secondary smelting and litharge making industry.

직업적 연폭로가 일부 신경행동학적 검사수행에 미치는 영향

안익수,안현철,김용배,리갑수,김화성,황규윤,최은,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

In order to investigate that blood lead levels of lead exposed workers is associated with decreased performance in neurobehavioral tests in adults, we investigate 78 workers in a battery industry. Three items among neurobehavioral core test battery of World Health Organization, including digit span, Santa ana dexterity, digit symbol were administered to workers occupationally lead exposed workers by operational guide for the WHO neurobehavioral core test battery. Blood lead (PbB) and zinc protoporphyrin in whole blood (ZZP) were selected as an index of lead exposure. Detailed occupational history such as exposed agent, age, work duration, smoking and drinking habits, were recorded as confounding factors. After controlling age, the results of digit span and digit symbol among the neurobehavioral tests were significant contributed by PbB.

연작업자들에서 혈중 및 골중 연량과 조혈기능 지표 사이의 관련성에 ALAD 다형질성이 미치는 영향

김화성,이성수,김용배,황보영,리갑수,안규동,장봉기,이병국 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 혈중 연량 및 골중 연량과 조혈기능의 생화학적 지표사이의 관련성에 f-aminolevinicacid dehydratase 효소의 유전형질의 다형질성이 어떠한 영향을 주는지를 알아보고자 연작업자 450명을 연폭로군으로 연폭로가 없는 85명을 대조군으로 선정하였다. 방 법 : 조혈기능의 생화학적 지표로는 혈중ZPP, 요중 ALA, 혈색소 및 혈구용적치를 택하였고, 이들 상호관계에 영향을 줄 수 있는 교란변수로서 성, 연령, BMI, 흡연습관, 음주습관 및 연작업자들의 경우 연폭로 기간을 조사하였다. 결 과 : 동형유전형질인 ALADI을 가진 연작업자들은 407명으로 전체의 90.4 %이었으며, 대조군에서도 77명으로 전체의 90.6 %로서 양군간에 차이가 없었다.동형 유전형질을 가진 연작업자들보다 이형 유전형질을 가진 연작업자들의 골중 연량과 혈중 ZPP의 평균값이 적었으나 통계적으로 유의하지 않았고 나머지 연구변수들의 평균도 유전형질의 구분에 따른차이는 없었다. 성, 직력, BMI, 흡연여부, 음주여부 등의 흔란변수를 통제한 후 혈중 연량 및 골중 연량과 ALAD유전형질이 혈색소에 미치는 영향을 구한 바 혈중 연량과 골중 연량만이 혈색소에 유의한 영향을 주었을뿐 ALAD 유전형질은 유의한 영향이 없었다. 반면에 혈구용적치의 경우에는 혈중 연량과 골중 연량 그리고 유전형질 모두가 유의한 영향을 주지 못했다.요중 ALA를 종속변수로 하고 관련 혼란변수를 통제한 후 혈중 연량과 ALAD 유전형질 그리고 양자의 교호작용을 독립변수로 한 회귀분석들에서 혈중 연량 및 골중 연량과 ALAD 유전형질 모두가 주효과(main effect) 및 교호작용(effect modifies-tion)이 유의하석 이형 ALAD 유전형질을 가진 연작업자들의 ALAU가 동형의 유전형질을 가진 연작업자들보다 상대적으로 낮은 값을 나타냈다.혈중 ZPP를 종속변수로 하고 관련 혼란변수를 통제한 후 혈중 연량과 ALAD 유전형질 그리고 양자의 교호작용을 독립변수로 한 회귀분석들에서 혈중연량 및 골중 연량과 ALAD 유전형질 모두가 음의주효과(maln effect)가 나타나서 이형 유전형질의 연작업자들의 혈중 ZPP가 동형 유전형질의 연작업자보다 상대적으로 적은 값을 나타냈으나 교호작용(effect modification)은 없었다. 결 론 : 상기 결과로 미루어 ALAD 유전형질은 혈색소에는 유의한 영향을 주지 못했으나 ALAU 및 혈중 ZPP에는 유의한 영향을 주어 동형의 유전형질인 연작업자보다 이형의 유전형질 연작업자들이 상대적으로 낮은 ALAU와 혈중 ZPP값을 보여주어이형유전형질이 연에 보호효과가 있다는 가설을 돼받침하였다 Objectives : To evaluate the effect of ALAD polymorphism on the relationship of blood and bone lead with hematologic biomarkers in lead exposed workers, 450 lead exposed workers and 85 non-lead exposed workers were selected. Methods : Blood lead and tibia bone lead were selected as parameters of lead exposure and blood ZPP, urinary ALA (ALAU) , hemoglobin (Hb) and hematocrit (Hct) were chosen as parameters of hematologic effect of lead exposure. Genotype of each subject was assayed and expressed as ALADI and ALAD2. Demographic information such as sex, age and personal habit of smoking and drinking were collected. Job duration of lead exposed workers was also obtained. Results : The overall prevalence of the variant allele, ALAD2 in lead exposed workers was 9.6 %(43 out of 450 lead workers) which was not differed from control workers (9.4 % : 8 out of 85) . The means of tibial lead and blond ZPP in lead workers with ALAD2 were lower than those of lead workers with ALAD1, but the differences were not statistically significant. After adjusting for possible confounders (sex, job duration, BMI, drinking and smoking status) only blood lead and bone lead contributed negatively to the level of hemoglobin with statistical significance without any contribution of ALAD genotype. On the other hand, no significant effect of blood lead, bone lead and ALAD genotype were observed on the level of hematocrit after controlling possible confounder. ALAD genotype in the multiple regression analysis of blood lead fed bone lead with log transformed urinary ALA (LogALAU) after adjusting for possible confounders showed significant main and interaction effect on LogALAU simultaneously, which resulted lower LogALAU in lead exposed workers of ALAD2 than ALAD1. It was also observed only main effect of ALAD gene type on blood ZPP after adjusting possible confounder resulting lower ZPP in lead workers of ALAD2 than ALAD1. Conclusions : With above results, it was found that ALAD polymorphism did not affect on the level of hemoglobin and hematocrit, but ALAD polymorphism made significant effect on the association of blood and bone lead with urinary ALA and blood ZPP. The lower urinary ALA and blood ZPP in ALAD2 lead workers suggested that ALAD2 genotype may be supportive for the protective effect of lead.

FET형 반도체 마이크로센서 개발(Ⅱ)

손병기,이흥락,박이순,조진호,이성필,최평,서화일,고성택,박재윤,서장수,김창수 경북대학교 센서기술연구소 1994 연차보고서 Vol.1994 No.-

현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte : H^+, K^+, Ca^2+, Na^+)분석 시스템, 용존가스(O_2, CO_2)분석 시스템의 개발을 중점적으로 추진하며, FET형 압력센서, 습도센서, 방사형 온도센서 등의 개별 FET형 센서에 관한 연구도 병행하고자 한다. Recently various kinds of sensors have been developed, being applicated to their own purpose. There are lots of difficulties to apply them to measurements in which the real-time monitoring is required without disturbing the surrounding environment. FET type semiconductor sensors, fabricated by the semiconductor integration technologies, have many advantages for their miniaturization, standardization, mass-production and in vivo/in situ monitoring. They also hold a very proper configuration for multi-functional sensors or integrated smart sensors, and wide availability by forming various kinds of physical or chemical sensing materials onto their sensing gates.

Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.