영지버섯 栽培에 있어서 푸른곰팡이 병균의 生長에 미치는 要因과 防除法

李鍾縕,張茂雄,李在成 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

本 實驗은 우리나라의 霙芝 버섯의 포트 栽培에서 發生하는 푸른곰팡이병의 原因茵의 규명과 그 防除法을 확립하고져 실시하였다. 實驗結果는 곰팡이 3種과 細茵 3種의 分離되었다. 이중 푸른곰팡이 症狀의 原因茵은 Trichoderma Roningi, T. lignorum, 未同定 1種임이 확인되었고, 발생빈도는 각각 60%, 30%, 10%이었다. T. lignorum은PDA 배지 Waksman's and Richard's solution에서 생장이 좋았고, 중성-알카리성에서는 生長이 不良한 반면 산성에서 生長이 좋았으며, 최적산도는 pH4였다. Trichoderma spp. 는 70℃에서 60分間, 80℃에서 30分間 열처리하므로서 완전히 사멸되었다. Three species of Trichoderma causing green mildew of Ganoderma lucidum were isolated from 6 spots of G. lucidum growing areas in Dae Gu. These are T. roningi, T. lignorum, and an unidentified species, and their frequencies of occurrence are 60%, 30%, and 10%, respectively. All of these species grew well in potato dextrose, Waksman's and Richard's solution, and preferred acidec(pH4.0) to neutral condditions. T. lignorum was killed when exposed for either 60minutes at 70℃, or for 30minutes at 80℃.

三天池의 環境과 微生物의 多樣性

한상국,이종운,배소현,장무웅,김시균 嶺南大學校 環境問題硏究所 1993 環境硏究 Vol.13 No.1

경상북도 경산시에 위치하고 있는 삼천지에 대한 환경요인 및 미생물상에 관하여 조사하였다. 삼천지 수계의 환경요인 분석결과, 그 평균값은 기온이 25.8℃, pH6.77, 경도 117.94mg/ℓ, 부유물질 4.5mg/ℓ, 용존산소량 3.3mg/ℓ, 생물학적 산소요구량 6.6mg/ℓ, 화학적 산쇼요구량 7.2㎎/ℓ, 염소이온 17.56㎎/ℓ, 황산이온 7.14㎎/ℓ로 나타났고, 중금속류는 Mn과 Zn만이 소량 검출되었으나 전반적인 오염도가 높아 수질환경 기준 IV급수에 해당하였다. 총 세균수는 3.32-8.85×?? No. inds/㎖의 분포를 나타냈고, 식물플랑크톤의 종구성은 규조류 33종, 녹조류 26종, 남조류 8종, 유글레나 13종, 와편모조류 6종 등 86종이 확인되었다. 규조류중 띠돌말과에 속하는 Fragilaria capucina, Synedra acus, S. familiaris 가 우점종이였다. Environmental factors and microflora of Samchun-impoundment in Kyongsan, Kyongpook were studied. Average data of environmental factors analysis are as followings : air temperature 25.8℃, pH 6.77, total hardness 117.94mg/ℓ, SS 4.5mg/ℓ, DO 3.3mg/ℓ, BOD 6.6mg/ℓ, COD 7.2mg/ℓ, ?? 17.56mg/ℓ, ?? 7.14mg/ℓ, Mn 0.05mg/ℓ, Zn 0.04mg/ℓ. Total bacteria in 4 site were 3.32×?? No. inds/㎖. Distribution and species of phytoplankton and zooplankton were 86 species including 33 species of bacillariophyceae, 26 of chlorophyta, 8 of cyanophyceae, 13 of euglenaceae, 6 of dinophyceae. Fragilaria capucina, Synedra acus, S. familiaris were dominant species.

Methylene Blue의 분자회합에 미치는 용매효과

박종회,박무순,박희억,서성원 충남대학교 1985 忠南科學硏究誌 Vol.12 No.2

A typical metachromatic dye, methylene blue(MB), has been found not to associate in ethanol, ethylene glycol, glycerol and 80 wt.% dioxane. The absorption spectra observed in the various kind of organic solvents are in good agreement with the calculated monomer spectra in both the shape and the absorption intensity. These results suggest that the water molecules play an important role in self-association of the dye molecules in aqueous solution. In this study, the changes in intensity and frequency shift of absorption spectra of MB are observed in the mixtures of water and organic solvents with the change in wt.% organic solvent. The discussions are made both upon the change in intensity and the frequency shift. The intensity changes are attributed to the change in the degree of association and the frequency shift is explained in terms of the interaction between the energy states of the MB molecules and the solvent polarity.

하이드록시프로필화 찹쌀 전분의 이화학적 성질 및 유과제조 특성

유철,최현욱,김종태,김동섭,최성원,박영준,백무열 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

표준균주(American Type Culture Collection)에 대한 Adenosine Triphosphate 측정기 평가

CHONG Moo-Sang 대한임상검사과학회 2025 대한임상검사과학회지(KJCLS) Vol.57 No.3

본 연구는 휴대용 adenosine triphosphate (ATP) 생물 발광 측정 장비인 Lumitester Smart를 활용하여, 임상미생물검사실에서 일반적으로 사용되는 표준균주들에 대해 ATP 발광값을 측정하고, 동일 균주에 대한 LuciPac A3 Surface와 LuciPac A3 Water의 채취 방법에 따른 상관관계를 분석함으로써 해당 장비의 위생 평가 도구로서의 적용 가능성을 검토하고, 결과 해석 시 고려해야 할 변수에 대해 알아보고자 하였다. McFarland 기준 농도에 따라 relative light unit (RLU) 값이 대부분의 균주에서 농도와 비례하여 감소하는 경향을 보여, Lumitester Smart (Kikkoman Biochemifa) 시스템이 일정 수준의 정량성을 가지고 있음을 확인할 수 있었다. 두 검체 채취 방법을 비교했을 때, LuciPac A3 Water는 대체로 LuciPac A3 Surface보다 높은 RLU 값을 기록하였다. 특히 Staphylococcus epidermidis, Pseudomonas aeruginosa, Staphylococcus aureus는 수계 환경에서 상대적으로 더 높은 ATP 수치를 보여, 수중 환경에서 세포 내 ATP가 더 효과적으로 추출되거나, 측정기 센서의 반응성이 더 높을 수 있음을 시사하였다. 의료 관련 감염의 중요성과 높아진 위생 기준에 비추어 볼 때, ATP 측정은 신속하고 정량적인 환경 위생 평가의 수단으로 그 활용도가 더 커질 것으로 기대된다. 향후 연구에서는 실제 환경에서의 ATP 수치와 감염률 간 상관관계를 분석하고, 병원 미생물과의 연관성에 관한 연구가 필요할 것으로 생각된다. This study assessed the applicability of the portable adenosine triphosphate (ATP) bioluminescence device, Lumitester Smart (Kikkoman Biochemifa), for hygiene monitoring in clinical microbiology laboratories. Standard reference strains were tested, and the ATP levels were measured using two sampling kits: LuciPac A3 Surface and LuciPac A3 Water. The relative light unit (RLU) values decreased proportionally at lower concentrations according to the McFarland standard, indicating the quantitative reliability of the system. In particular, LuciPac A3 Water generally produced higher RLU values than LuciPac A3 Surface. This trend was especially evident in Staphylococcus epidermidis, Pseudomonas aeruginosa, and Staphylococcus aureus, suggesting that ATP extraction or detection is more efficient under aqueous conditions. These findings emphasize the influence of sampling methods on result interpretation. Considering the increasing importance of healthcare-associated infections and the demand for rapid and reliable hygiene assessment methods, ATP measurement technology has potential as a quantitative and real-time monitoring approach. Future research should focus on investigating the correlation between the ATP levels and infection incidence in real-world healthcare environments, as well as exploring the associations with specific pathogenic microorganisms to establish the broader clinical utility of this approach.

국내 위 생검에서 헬리코박터 파일로리 감염의 클라리스로마이신 내성률

Moo-Sang CHONG,Kyutaeg LEE 대한임상검사과학회 2025 대한임상검사과학회지(KJCLS) Vol.57 No.2

Gastric biopsies or tissue samples (4,698 samples) were collected from 50 local clinics or hospitals between January 2022 and December 2024 for the molecular detection of Helicobacter pylori and clarithromycin resistance using 23S ribosomal RNA genes. The clarithromycin resistance rates were analyzed. The patient’s age, sex, and regional distribution in Korea were investigated to determine the A2142G and A2143G mutations related to clarithromycin resistance. The clarithromycin resistance rate accounted for 32.6% of the 4,698 patients, with 40.5% and 25.7% in female and male, respectively. Among the mutation types, A2143G showed a higher frequency than A2142G in male and female. The resistance rate was higher in female than in male. In particular, the resistance rate in those showing the A2143G mutation in female increased every year. The mutation frequency was higher in female than in male. The A2143G mutation had a higher frequency than the A2142G mutation. In particular, the frequency of A2142G in female tended to increase yearly. The frequency showed differences according to age and region. Therefore, when treating H. pylori, the treatment outcome and the occurrence of resistance must be considered by referring to the clarithromycin resistance rate.

자궁경부 감염에서 고위험 인간 유두종 바이러스 유전자형의 음성 전환율 및 재발률

Moo-Sang CHONG,Wonseo PARK,Kyutaeg LEE 대한임상검사과학회 2024 대한임상검사과학회지(KJCLS) Vol.56 No.4

자궁경부암은 전 세계적으로 중요한 건강 문제이다. 고위험인유두종 바이러스(high-risk human papillomavirus, HPV) 감염은 HPV-16, HPV-18과 같이 자궁경부암의 원인에 중요한 역할을 하는 것으로 밝혀졌다. HPV 감염의 90%는 바이러스에 노출된 후 12∼24개월 내에 자연스럽게 제거되거나 비활성화되지만, 자궁경부암으로 진행될 위험을 증가시키는 유전자형에 따른 고위험 HPV의 지속성에 대한 보고는 많지 않다. 본 논문은 고위험 HPV 자궁경부 감염에서 고위험 HPV 유전자형에 따른 음성 전환율과 재발률을 분석했다. 5년 동안 고위험 HPV의 음성 전환율은 평균 53.0%였으며, 감염된 HPV 유전자형의 수가 많을수록 음성 전환율이 감소했다. 음성 전환까지 걸리는 시간은 감염된 HPV 유전자형 수가 많을수록 증가했으며, 단일감염 환자에서는 연령과 유전자형에 따라 상이한 결과를 보였다. 5년 동안 재발률은 평균 10.7%였으며, 감염된 HPV 유전자형의 수가 많을수록 재발률이 감소하고, 재발까지 걸리는 시간도 줄어들었다. 동일 유전자형에서의 재발률은 유전자형에 따라 달랐다. 이러한 결과는 HPV 감염 환자의 치료효과 판정과 재발률에 대한 예후 추정의 보조 수단으로 사용할 수 있을 것으로 생각된다. Cervical cancer is a major health issue globally. High-risk human papillomavirus (Hr HPV) infections, such as HPV16/18, play a crucial role in the cause of cervical cancer. Ninety percent of HPV infections are cleared or become inactive within 12 to 24 months of exposure to the virus. On the other hand, there are a few reports about the persistence of Hr HPV according to each genotype, which increases the risk of progression to cervical cancer. Therefore, this study analyzed the negative conversion rates and the relapse rates according to each Hr HPV genotype in Hr-HPV cervical infections. The negative conversion rate of Hr HPV averaged 53.0% for five years and decreased with the number of infected HPV genotypes. The time to negative conversion increased with the number of infected HPV genotypes and varied with age and genotypes in single-infected patients. The relapse rates averaged 10.7% for five years, decreased with the number of infected HPV genotypes, and the time to relapse decreased with the number of infected HPV genotypes. The rates of relapse with the same genotype varied according to the genotype. These results can be used as an adjunct to prognostic estimates of the cure and recurrence rates in HPV-infected individuals.

대중음식점 일회용 물티슈의 세균 오염도 조사

정무상 ( Moo Sang Chong ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.3

This study, funded by Jeju Special Self-Governing Province, evaluated the bacterial contamination in disposable wet wipes used in restaurants, coffee shops, and bakery shops. The materials used are 55 disposable wet wipes collected from general restaurants. The number of average bacteria found was 4.21×10³ CFU/mL in 50 disposable wet wipes; in the other five disposable wet wipes, no bacterial contamination was found. The highest number of bacteria was measured as 16.67×10³ CFU/mL. Total bacterial count of separated are 71 strains. Among the 71 identified strains, 15 were Staphylococcus aureus and 3 were Pseudomonas aeruginosa. This can be an important problem. This study measured the level of bacterial contamination and identified the strains isolated in wet wipes. This study may be important as it provides the basis for establishing sanitation standars for wet wipes.

상업용 사료 급여 반려견 배설물에서 배양된 장내 미생물의 분류

정무상(Chong, Moo-Sang) 한국보건기초의학회 2020 한국보건기초의학회지 Vol.13 No.2

The purpose of this study was to investigate the microorganisms found in the intestines of dogs fed commercial feed though the aerobic culture of dog excrement. 16 specimens of pet dog feces were cultured employing BAP, MaC, and EMB medium. Strains of Escherichia coli were detected in all the cultured specimens, along with a few other bacteria of significance. All 16 samples were identified as containing multiple E. coli strains using a API20E kit. Two samples showed a swarming phenomenon in the BAP medium that was identified as a Proteus mirabilis strain. In 10 samples, only E. coli species were identified, while E. coli species and P. mirabilis species were identified in 2 samples. A small number of E. coli and gram-positive bacteria were detected in 3 specimens, and a small number of E. coli and gram-positive rods were detected in one specimen. It has been shown that E. coli is predominantly distributed in the feces of dogs fed with commercial feed. The fact that E. coli is distributed in the feces of dogs is of concern as it can cause serious problems such as water pollution and food poisoning.

대중음식점 일회용 물티슈의 세균 오염도 조사(2)

정무상 ( Moo-sang Chong ) 대한임상검사과학회 2022 대한임상검사과학회지(KJCLS) Vol.54 No.1

Disposable wet wipes provided by general restaurants in the Jeju Special Self-Governing Province were evaluated for compliance with the labeling requirements and the degree of bacterial contamination set by the Ministry of Food and Drug Safety. Of the 32 types of disposable wipes, five types of disposable wipes did not indicate the name and location of the business office of the manufacturer. Only 12 types of wet wipes were marked with the date of manufacture and expiration date. Bacterial contamination was seen in 9 types of wet wipes through bacterial culture but these were absent in the remaining 23 types (71.9%). The results of the antimicrobial susceptibility test for the 9 identified strains showed no methicillin-resistant coagulase-negative staphylococci (MRCNS) or multidrug-resistant bacteria. Through this study, it is recommended that the date of manufacture and expiration date must be indicated on the surface of disposable wet wipes designated as hygiene products, and clean uncontaminated wet wipes must comply with storage standards. In addition, the results are expected to contribute to the enhancement and improvement of hygiene management supervision, personal hygiene management, and an improvement in national health.