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RISS 인기검색어
- 검색키워드
- 저자 : H. Sasaki (검색결과 46 건)
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Sasaki, H.,Horiguchi, K.,Takahashi, T. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.1
The effect of different feeding ratios of concentrate to roughage on ruminal lipid synthesis in vitro was examined. Three sheep fitted with a rumen fistula were fed three different ratios (8:2, 4:6 and 0:10) of concentrate and roughage, and their rumen liquor were used for incubation. $^{13}C$-labeled glucose or sodium acetate as substrate was added to cultures of rumen liquor, and they were incubated for 6 h. The total lipid in the culture of the rumen liquor was extracted, and the percentage of $^{13}C$ excess was analyzed. The percentage of $^{13}C$ excess recovered when incubated with glucose increased with increased ratio of concentrate in the diet. The values of cultures incubated with glucose were higher than those incubated with sodium acetate except the roughage-only feeding. In the roughage-only diet, the percentage of $^{13}C$ excess when incubated with sodium acetate was highest of all diets. The recovery percentage of $^{13}C$ from glucose increased with increased ratio of concentrate. The recovery percentage of $^{13}C$ from sodium acetate addition in only roughage feeding was highest among the three diets. The recovery percentage of $^{13}C$ from glucose was markedly higher than that of sodium acetate addition in all feedings. The results indicate that high concentrate feeding facilitates lipid synthesis by rumen microorganisms, and that glucose may be the precursor for lipid synthesis rather than acetic acid.
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The catalytic activities of nanoclusters dispersed on apatite
J.Ichihara,K.Iteya,H.Kawaguchi,Y.Sasaki,H.Nakayama,S.Yamaguchi 한양대학교 세라믹연구소 2003 Journal of Ceramic Processing Research Vol.4 No.1
Cetylpyridinium phosphomolybdates (Q3[PMo12O40], Q=CetylPy) dispersed on apatite catalyzed H2O2-oxidations of organic compounds under solid-phase conditions without organic solvent. FT-IR and 31P solid-state NMR spectroscopic studies showed the formation of peroxo species in our solid-phase system, which was different from the peroxo type of CetylPy3{PO4[Mo(O)(O2)2]4} (PMo4) known as an active species in the conventional liquid-biphase system. The catalytic activities of them were compared in the solid-phase system.
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Sasaki, H.,Horiguchi, K.,Takahashi, T. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.7
The effects of different feeding ratios of concentrate (C) and roughage (R) on balance of long chain fatty acids and microbial fatty acids in the rumen of sheep were investigated. The diets were divided into 8:2 (concentrate feeding), 4:6 (middle mixture) and 0:10 (roughage feeding) ratios (C:R). Duodenal digesta was collected through 24 hours after feeding. Biohydrogenation rate, total duodenal flow of fatty acids and microbial fatty acids were measured. Total duodenal flow of fatty acids was significantly (p<0.05) increased with increasing concentrate. Total duodenal flow of fatty acid was greater than intake of fatty acid in all diets. In comparison with intake of each fatty acid, duodenal flow of stearic acid ($C_{18:0}$) remarkably increased in all diets. Biohydrogenation rate for total C18 unsaturated fatty acids in the rumen tended to increase (p<0.10) when sheep were fed the middle mixture. In particular, biohydrogenation rate of linoleic acid ($C_{18:2}$) and linolenic acid ($C_{18:3}$) with the middle mixture were highest (p<0.05) compared with other diets. Duodenal flow of protozoal fatty acids was significantly (p<0.05) increased with the increased supply of concentrate. That of bacterial fatty acids was significantly (p<0.05) increased with both concentrate diets compared with the roughage feeding diet. $C_{18:0}$ occupied the greater part of both protozoal and bacterial fatty acids in all treatments. Results indicated that biohydrogenation of free unsaturated fatty acids was actively carried out when the middle mixture diet was supplied, and that microbial uptake and synthesis of fatty acids were accelerated by adding the supply of concentrate.
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Contamination and Bioconcentration of Perfluorinated Compounds in Main River Ecosystems of Korea
N. H. Lam,C.R. Cho,M.S. Chang,B.C. Lee,P.J. Kim,K. Sasaki,H.S. Cho 한국해양환경·에너지학회 2012 한국해양환경공학회 학술대회논문집 Vol.2012 No.11
Water, sediment, plankton, blood and liver of crucian carp (Carassius carassius) and mandarin fish (Siniperca cherzeri) samples from 5 freshwater ecosystems in 3 major rivers of Korea including Nam Han River, Nakdong River and Yeongsan River were collected together with medaka fish (Oryzias latipes) and water in their 5 natural habitats. The samples were analyzed to determine the occurrence and contamination level of perfluorinated compounds (PFCs). The mean total concentration of detected PFCs was in the ascending order of water, sediment, plankton, crucian carp and mandarin fish. Longchain PFCs (≥C8) was widely detected and occupied 99.32% of total PFCs concentration. Perfluorooctane sulfonate (PFOS) was the dominant PFC in most of the ecosystem components with a maximum concentration in water of 6.25 ng/L and in fish of 273 ng/g wet wt. The results also demonstrated that concentration of PFOS and total PFCs in blood of crucian carp and mandarin fish were higher than those in their liver. PFOS concentrations detected in water samples were lower than the water quality criteria values for aquatic organisms protection, which were reported in Giesy et al., 2010. A positive linear regression (r²=0.99, p<0.05) and a trophic magnification factor of 28.25 were observed between PFOS concentrations and trophic levels of collected biota. The mean bioconcentration factor (BCF) of PFOS were extended from 14,745±21,366 (in plankton) to 517,476±705,036 (in crucian carp blood). According to the method of Moermond et al., 2010 and the received BCF of sensitive medaka fish in this study, we suggested the MPCsp,water of 0.083 ng/L for PFOS in Korea freshwater ecosystems.
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Ochiai, H.,Park, H.M.,Sasaki, R.,Okumura, J.,Muramatsu, T. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.1
Factors affecting gene gun-mediated expression of the human erythropoietin gene were investigated in primary cultured oviduct cells from laying hens. The human erythropoietin gene was transfected by a gene gun method at $1.25{\mu}g$ per dish, and cultured in a synthetic serum-free medium for 72 hrs. The concentration of human erythropoietin mRNA was determined by RNA : RNA solution hybridization. In experiment 1, the effect of changing the shooting pressure of DNA-coated microparticles with nitrogen gas was tested at 20 and $60kgf/cm^2$. The results showed that the erythropoietin mRNA concentration was significantly higher at 60 than $20kgf/cm^2$. In experiment 2, the effects of supplementing the medium with fetal calf serum at 10%, and raising the shooting pressure from 60 to $80kgf/cm^2$ on the cell number and erythropoietin gene expression were examined. Although supplementation with fetal calf serum significantly increased the cell numbes compared with no supplemented controls (p < 0.05), erythropoietin mRNA concentration per $10^3$ cells was not affected. Raising the shooting pressure from 60 to $80kgf/cm^2$ did not affect either of the parameters, In experiment 3, the effect of supplementing ascorbate 2-phosphate at 0.5 mM was tested. The results indicated that the ascorbate supplementation significantly increased the cell number (p < 0.05), and tended to increase erythropoietin mRNA concentration (p < 0.1). Thus, for human erythropoietin gene expression by using the gene gun method, shooting pressure with nitrogen gas should be sufficient at $60kgf/cm^2$ and supplementation with ascorbate phosphate would be useful to enhance not only the cell proliferation but also erythropoietin gene expression.
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헤테로코어형 광파이브 센서를 이용한 환경 모니터링 시스템 개발에 관한 연구
佐?木博幸(H. Sasaki),渡?一弘(K. Watanabe),최용운(Y. Choi),김영복(Y. B. Kim) 한국동력기계공학회 2006 한국동력기계공학회 학술대회 논문집 Vol.- No.-
A multi purpose environmental monitoring system has been developed as a commercially available standard using the technique of hetero-core spliced fiber optic sensor, for the purposes of monitoring large scaled structures and preserving natural environments. The monitoring system has been tested and evaluated in a possible outdoor condition in view of the full scaled operation at actual sites to be monitored. Additionally, the developed system in this work conveniently provides us with various options of sensor modules intended for monitoring such physical quantities as displacement, distortion, pressure, binary states, and liquid adhesion. Two channels of optical fiber line were monitored, in each of which three displacement sensor modules were connected in series, in order to examine the performance to a pseudo-cracking experiment in the outdoor situation, and to clarify temperature influences to the system in terms of the coupling of optical connectors and the OTDR stability. The pseudo-cracking experiment successfully observed the actually given cracks by means of calculation based on the detected displacement values and their geometrical arrangement of the used sensor modules. The temperature change ranging from 10 to 20 ℃ resulted from the 10-days free running operation was found to influence the system stability of ±10㎛ mainly because of the coupling instability of the used optical connectors. It was found that fusion splicing instead of the use of connectors reduced the fluctuation down to ±211m. The specification and performances of various option modules have been demonstrated to show the capability of inspecting various physical quantities by the use of the single system which would be suitable for multi-purpose environmental monitoring.
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DEVELOPMENT OF PVC WAVEGUIDE SENSOR FOR MEASUREMENT OF Ca^(++) CONCENTRATION
Kim, Kyung H.,Minamitani, H.,Hisamoto, H.,Suzuki, K.,Kang, S.W.,Sasaki, K. 경북대학교 센서기술연구소 1994 센서技術學術大會論文集 Vol.5 No.1
In this paper, a PVC optical thin film waveguide sensor is proposed which can select a specific ion and measure its concentration depending on materials added into waveguide. In order to investigate the applicability of this sensor to Ca^(++) measurement, the waveguide was fabricated with poly vinyl chloride (PVC), bis ethyl hexyl phthalate (BEHP), lipophilic anionic dye (LAD-3), and ionophore (K23E1) which reacts on Ca^(++). For this purpose, we applied the sensor to sample solution whose CaCl_(2) concentration was gradually altered. In this experiment, we found that this thin film waveguide possessed good light propagation. In addition, it showed the chemical reaction to Ca^(++) enough to select Ca^(++) and measure its concentration. These findings suggested that the proposed PVC optical thin film waveguide ion sensor was very effective in selecting Ca^(++) and measuring its concentration. Also, it will be expected that this sensor is applicable to various ions other than Ca^(++).
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The Embedment of a Metadata System at Grid Farms at the Belle II Experiment
S. Ahn,J. H. Kim,T. Huh,S. Hwang,조기현,H. Jang,B. K. Kim,H. Yoon,J. Yu,Z. Drasal,T. Hara,Y. Iida,R. Itoh,G. Iwai,N. Katayama,Y. Kawai,S. Nishida,T. Sasaki,Y. Watase,T. Uglov,R. Fruhwirth 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.4
In order to search for new physics beyond the standard model, the next generation of B-factory experiment, Belle II will collect a huge data sample that is a challenge for computing systems. The Belle II experiment, which should commence data collection in 2015, expects data rates 50 times greater than that of Belle. In order to handle this amount of data, we need a new data handling system based on a new computing model, which is a distributed computing model including grid farms as opposed to the central computing model using clusters at the Belle experiment. We have constructed a metadata system and embedded the system in the grid farms of the Belle II experiment. We have tested the system using grid farms. Results show good performance in handling such a huge amount of data.
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