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Stemmed DNA nanostructure for the selective delivery of therapeutics
Jin, H.,Kim, M. G.,Ko, S. B.,Kim, D. H.,Lee, B. J.,Macgregor, Jr., R. B.,Shim, G.,Oh, Y. K. The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.16
<P>DNA has emerged as a biocompatible biomaterial that may be considered for various applications. Here, we report tumor cell-specific aptamer-modified DNA nanostructures for the specific recognition and delivery of therapeutic chemicals to cancer cells. Protein tyrosine kinase (PTK)7-specific DNA aptamer sequences were linked to 15 consecutive guanines. The resulting aptamer-modified product, AptG15, self-assembled into a Y-shaped structure. The presence of a G-quadruplex at AptG15 was confirmed by circular dichroism and Raman spectroscopy. The utility of AptG15 as a nanocarrier of therapeutics was tested by loading the photosensitizer, methylene blue (MB), to the G-quadruplex as a model drug. The generated MB-loaded AptG15 (MB/AptG15) showed specific and enhanced uptake to CCRF-CEM cells, which overexpress PTK7, compared with Ramos cells, which lack PTK7, or CCRF-CEM cells treated with a PTK7-specific siRNA. The therapeutic activity of MB/AptG15 was tested by triggering its photodynamic effects. Upon 660 nm light irradiation, MB/AptG15 showed greater reactive oxygen species generation and anticancer activity in PTK7-overexpressing cells compared to cells treated with MB alone, those treated with AptG15, and other comparison groups. AptG15 stemmed DNA nanostructures have significant potential for the cell-type-specific delivery of therapeutics, and possibly for the molecular imaging of target cells.</P>
Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor
Jin, L.,Ko, S. R.,Cui, Y.,Lee, C. S.,Oh, H. M.,Ahn, C. Y.,Lee, H. G. Kluwer Academic Publishers [etc.] 2017 Antonie van Leeuwenhoek Vol.110 No.1
<P>A polyphasic taxonomic study was carried out on strain EBR-8-1(T) isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9(T) (99.1 %), Pusillimonas soli MJ07(T) (97.3 %), Pusillimonas ginsengisoli DCY25(T) (97.2 %), and Pusillimonas harenae B201(T) (96.8 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C-16:0, C-17:0 cyclo and C-19:0 cyclo omega 8c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3 mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1(T) should be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1(T) (=KCTC 42353(T) = JCM 30463(T)).</P>
김유광,박근영,이강이,진영권 한국항공운항학회 2003 한국항공운항학회지 Vol.11 No.2
In this paper, the design data of an aircraft generator have been reviewed to evaluate the compliance with the airworthiness standards, and the technical procedure to ensure safety and reliability of an alternate-current generator by the tests and computations have been developed.
홀스타인 수소에 있어서 Chromium Picolinate 의 첨가가 성장성적, 혈액성상 및 도체품질에 미치는 영향
홍중산,김명국,김윤학,한승은,이홍구,이현준,최윤재 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.4
본 시험은 성장단계별로 서로 다른 수준의 CrP를 첨가하여 성장성적, 혈액성상 및 도체품질에 미치는 영향을 규명하고자 실시하였다. 시험 Ⅰ에서 일당증체, 일당건물섭취량 및 사료효율에는 유의적인 차이가 없었다(p<0.05). 혈액성상에서 혈장 Glucose와 PUN의 농도에는 영향이 없었지만 혈중 인슐린의 농도는 0.05% CrP첨가구가 대조구의 2배되는 1.47ng/㎖ 인데 반하여 혈중 NEFA의 농도는 59.00 mEq/㎗ 로 유의적으로 낮게 나타났다(P<0.05). 이는 인슐린이 농도의 증가 및 인슐린의 활성의 증가로 인하여 지방합성대사가 활발히 진행된 결과로 사료된다. 이러한 결과를 도체분석결과가 뒷받침 해주고 있는데 비록 통계적인 유의차는 없지만 0.05% CrP 첨가구에서는 첫 60일간, 0.025% CrP 첨가구에서는 첫 120일간 대조구보다 우수한 성적을 나타냈다. 혈장 Glucose와 PUN의 농도에는 영향이 없었다. 통계적인 유의차는 없었지만 혈 중 인슐린의 농도는 0.025% CrP 첨가구는 대조구보다 8.88% 높은 1.35ng/㎖로 나타났고 0.05% CrP 첨가구는 오히려 1.13ng/㎖로 대조구보다 낮게 나타났다. NEFA의 농도는 시험 Ⅰ에서와 같은 차이는 없었지만 CrP 첨가수준이 증가할수록 감소하는 추세를 나타냈고 등지방두께는 일정한 변화추세를 보이지 않았다. We conducted two experiments to evaluate the effects of chromium picolinate(CrP) on growth performance, carcass characteristics and plasma components in Holstein bulls. In trial Ⅰ, eight finishing Holstein bulls(300±6.99㎏) were allocated to 2 treatments(control and 0.05% CrP) with 4 replication for 10-months. In results, growth performance was not affected by CrP addition. The plasma insulin concentration in 0.05% CrP group was about 2 times higher than the control group of Holstein bulls. The levels of plasma NEFA were significantly decreased to 59.00 mEq/㎗ with 0.05% CrP treatment(P<0.05), but the levels of plasma glucose and PUN were not altered by 0.05% CrP treatment. The grade of carcass was not different between control and 0.05% CrP group, but back fat thickness in 0.05%, CrP group was increased in 22.33% compared with control group. In trial 2, fifteen growing-finishing Holstein bulls(160±4.63㎏) were allocated to 3 treatments(control, 0.025% CrP and 0.05% CrP) with 5 replication for 14-months. During the overall experimental period, growth performance was not affected by CrP levels. The levels of hormone and metabolites were not affected by CrP supplementation. The carcass characteristics were not different between control and treatment. These results show that the CrP may have no effects for beef cattle production because of degradation of CrP conjugation in the rumen. However treatment of short term provide a possibility the effects of development for lipogenesis.
톱밥 발효사료 급여 및 분말어유의 첨가사료가 돈육의 이화학적 성질에 미치는 영향
이정일,문점동,박구부,박범영,박태선,황보종,이한기,진상근,김영직 한국축산학회 1997 한국축산학회지 Vol.39 No.5
This study was conducted to investigate the influence of pork quality pigs which were fed with diets supplemented with fermented sawdust and sardin powder oil. The pigs were randomly assigned to one of the four treatments: a), control (commercial feed); b), T1 (30% fermented sawdust); c), T2 (10% sardin powder oil) d), T3 (30% fermented sawdust, l0% sardin powder oil and 30% limited amino acid) from 30 kg to 110 kg B. Wt. and slaughtered. The samples were stored at 0±1 C. The physico-chemical properties were determined at the dust of 2, 4, 6, 8 and 15. For the loin the pH's of all the treatments on the 15th day were higher than those of the 2nd day, and that of TI was higher than those of the others. The total moisture contents of all treatments decreased with the storage (P$lt;0.05), and there were no significant differences among treatments on the 15th day. The water soluble proteins of all treatments increased with time and were highest on the 8th day and decreased gianibicanth thereater. T1 had significantly higher water soluble protein than others(P$lt;0.05). The salt soluble proteins of all treatments increased with lure the days(P$lt;0.05). T1 had higher salt soluble protein than others during the storage period(P$lt;0.05). The brittleness, hardness, cohesiveness, gumminess and chewiness of all the treatments were not significantly different among during the period, but elasticity of them increased after from the 2nd day. The brittleness of control was higher than that of the others on the 15th day, and the hardness and chewiness of control were higher than those of the others on the 6th day(P$lt;0.05). Forthebelly the pH's of all the treatments on the 15th day were higher than those of the at 2nd day(P$lt;0.05). T1 showed higher pH than the others during the period(P$lt;0.05). The total moisture contents of control and T1 were significantly decreased with time and there were no significant differences between T2 and T3. T1 showed the highest value. The water soluble proteins of all the treatments decreased with time and was lower on the 15th day(P$lt;0.05). That of control and T1 was significantly higher than that of others on the 2nd day, but there were no significant differences among the treatments on the 15th day. The salt soluble proteins of all treatments increased with time (P$lt;0.05), and they were highest on the 15th day. T1 had significantly higher salt soluble protein than the others on the 8th and 15th days.