Dysregulated lncRNA and mRNA may promote the progression of ischemic stroke via immune and inflammatory pathways: results from RNA sequencing and bioinformatics analysis

Wang Yingshuang,Feng Feifei,Zheng Pingping,Wang Lijuan,Wang Yanjun,Lv Yaogai,Shen Li,Li Kexin,Feng Tianyu,Chen Yang,Liu Zhigang,Yao Yan 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Long non-coding RNAs (lncRNAs) are widely involved in gene transcription regulation and which act as epigenetic modifiers in many diseases. Objective: To determine whether lncRNAs are involved in ischemic stroke (IS), we analyzed the expression profile of lncRNAs and mRNAs in IS. Methods: RNA sequencing was performed on the blood of three pairs of IS patients and healthy controls. Differential expression analysis was used to identify differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs). Based on the co-expression relationships between lncRNA and mRNA, a series of bioinformatics analysis including GO and KEGG enrichment analysis and PPI analysis, were conducted to predict the function of lncRNA. Results: RNA sequencing produced a total of 5 DElncRNAs and 144 DEmRNAs. Influenza A pathway and Herpes simplex infection pathway were the most significant pathways. EP300 and NFKB1 were the most important target proteins, and Human leucocyte antigen (HLA) family were the key genes in IS. Conclusions: Analysis of this study revealed that dysregulated lncRNAs in IS may lead to IS by affecting the immune and inflammation system.

A facile technique to prepare cross-linked enzyme aggregates using p-benzoquinone as cross-linking agent

Anming Wang,Fangkai Zhang,Feifei Chen,Meizhang Wang,Haifeng Li,Zhaowu Zeng,Tian Xie,Zhenming Chen 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.4

To obtain robust and thermo-stable enzyme aggregates, p-benzoquinone was used as cross-linker and bovine serum albumin (BSA) as crowding macromolecules to prepare cross-linked enzyme aggregates (CLEAs) of lipase. Effects of cross-linking time and cross-linker content on the activity, thermal stability and characteristics of enzyme aggregates were examined carefully. It was observed that when the content of p_benzoquinone was 5 mM and amount of BSA was 125% of that of lipase (w/w), the specific activity of cross-linked co-aggregates of lipase and BSA was 79.8 U mg^−1, 2.44-fold of that of cross-linked enzyme aggregates of lipase without BSA. Moreover, after heat treatment for 96 h at 50℃ , the CLEAs prepared with this facile routine kept 75.18% of their initial activity, 5.01-fold more than that of the just CLEAs using glutaraldehyde. Furthermore, BSA macromolecules in lipase CLEAs enhanced the catalytic efficiency of free and just lipase CLEAs without BSA by 1.45 and 2.83 times, respectively. The proposed crosslinking technique would rank among the potential strategies for efficiently preparing robust and thermo-stable enzyme aggregates.

Design of Intelligent Management and Service System for Gas Valve

Xiaoli Wang,Feifei Wang,Yuhou Song,Guirong Zhang,Shaohui Wang 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.5

This paper introduces a design scheme of intelligent gas valve management and service system based onInternet. This scheme adds sensor and general packet radio service (GPRS) modules to the traditional gas valveand establishes communication connection between gas valve and the server through wireless packetcommunication technology, which makes the traditional gas valve have the networking ability. Compared withthe traditional gas valve management and service business, the method proposed in this paper is moreconvenient and efficient.

Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis

Dachuan Fan,Xiangdong Wang,Min Wang,Yang Wang,Liang Zhang,Ying Li,Erzhong Fan,Feifei Cao,Koen Van Crombruggen,Luo Zhang 대한천식알레르기학회 2016 Allergy, Asthma & Immunology Research Vol.8 No.3

Purpose: Group 2 innate lymphoid cells (ILC2s) are a novel population of lineage-negative cells that induce innate type 2 responses by producing the critical Th2-type cytokines IL-5 and IL-13 in response to IL-25 and IL-33 stimulation. ILC2s accumulation in the peripheral blood of patients with allergic rhinitis (AR) is controversial; the precise role of ILC2s in the immunopathogenesis of AR is still not clear. We investigated the role of ILC2s in phenotypic AR sensitized to distinct allergens. Methods: Flow cytometric analysis of the peripheral blood of 7 healthy controls (HCs), 9 patients monosensitized to house dust mite (HDM), and 8 patients monosensitized to mugwort was performed to quantify ILC2s frequency. Peripheral blood mononuclear cells (PBMCs) were isolated from HDM-AR and mugwort-AR patients, and Lineage- and Lineage+ cells were separated using a fluorescence-activated cell sorter (FACS). IL-5 and IL-13 levels in the supernatants of PBMCs, and Lineage- and Lineage+ cells stimulated with IL-25 and/or IL-33 combined with IL-2 in vitro were assessed using the Milliplex magnetic bead kit. Results: The percentage of ILC2s was significantly elevated in HDM-AR patients compared to mugwort-AR patients and HCs, while no significant difference was found between mugwort-AR patients and HCs. IL-33±IL-25 plus IL-2 induced a significantly greater release of IL-5 and IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. IL-25 plus IL-2 also induced a significantly greater release of IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. Stimulation with IL-33 and/or IL-25 combined with IL-2 also induced a significantly greater IL-5 and IL-13 release from Lineage- cells compared to Lineage+ cells. Conclusions: AR patients sensitized to HDM or mugwort allergen have distinct phenotypic and functional profiles in ILC2s frequencies. ILC2s mediate major type 2 immunity in the development of HDM-AR and may be a potential therapeutic target.

Intracerebral Hemorrhage Progression Score: A Novel Risk Score to Predict Neurological Deterioration after Intracerebral Hemorrhage

Ruijun Ji,Linlin Wang,Feifei Ma,Wenjuan Wang,Yanfang Liu,Runhua Zhang,Dandan Wang,Jiaokun Jia,Hao Feng,Gaifen Liu,Yi Ju,Jingjing Lu,Xingquan Zhao 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.2

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Changes in Carbon, Nitrogen and Phosphorus Stoichiometry with Cut and Organ in Lucerne

Feifei You,Zhennan Wang,Jiaoyun Lu,Huimin Yang 한국초지조사료학회 2016 한국초지조사료학회 학술대회논문집 Vol.2016 No.08

Exergy analysis and multi-objective optimisation for energy system: a case study of a separation process in ethylene manufacturing

Feifei Shen,Meihong Wang,Lingxiang Huang,Feng Qian 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-

In chemical industry, most processes face the challenge of high energy consumption. The approachpresented in this study can reduce the energy footprint and increase efficiency. The energy system of aseparation process in ethylene manufacturing is used to demonstrate the effectiveness of the approach. The chilling train system of the separation process in a typical ethylene plant consumes most cooling andprovides appropriate feed for distillation columns. The steady state simulation of system was presentedand the simulation results were proved accurate. The conventional exergy analysis identifies thatDephlegmator No.1 (a heat exchange and mass transfer device) has the highest exergy destruction(1401.28 kW). Based on advanced exergy analysis, Dephlegmator No.1 has the highest rate of avoidableexergy destruction (89.04 %). Finally, a multi-objective optimisation aiming to maximise system exergyefficiency and to minimise operational cost was performed and the Pareto frontier was obtained. Themulti-objective optimized exergy efficiency is 79.53 % (improved by 0.61 %) and the operational cost is0.02031 yuan/kg (saved by 11.19 %). This study will guide future research to reduce energy consumptionin process manufacturing.

Ethanol dry reforming over ordered mesoporous Co-Zn composite oxide for syngas production

Feifei Li,Jiale Dong,Mingyue Wang,Xingtao Lin,Weijie Cai,Xianyun Liu 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7

This work is mainly concerned with the synthesis of Co-Zn nanocomposites by employing MCM-41 silicaas hard template (CoZnO-HT) and its catalytic behavior toward to CO2 reforming with ethanol. The physicochemicalfeatures of the as-prepared catalysts were probed through various characterization techniques, including XRD, TEM,BET, H2-TPR, and XPS. Indeed, CoZnO-HT catalyst possessed a highly ordered mesostructure with similarity toMCM-41 template and a higher specific surface area (304m2/g) compared to the reference CoZnO-C sample (4.75m2/g) prepared by the conventional impregnation method. Consequently, CoZnO-HT exhibited good performance at lowtemperature, and full ethanol conversion could be achieved at 550 oC as well as the negligible formation of byproductacetone. In addition, this catalyst depicted good stability and no obvious deactivation was observed after 40 h time onstream tests under the stoichiometric feed ratio. Indeed, superior specific surface area and efficient mass transportwithin the mesopores might be critical factors assigned to better activity and stability for CoZnO-HT.

Isolation and identification of goose skeletal muscle satellite cells and preliminary study on the function of C1q and tumor necrosis factor-related protein 3 gene

Wang Han,He Ke,Zeng Xuehua,Zhou Xiaolong,Yan Feifei,Yang Songbai,Zhao Ayong 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/ enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose. Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs <i>in vitro</i>. Additionally, to explore the function of CTRP3 in goose SMSCs.Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression.Results: The goose SMSCs were successfully isolated and cultured. The expression of <i>Pax7</i> and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, <i>MyHC</i> and <i>MyoG</i>. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation.Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

Dynamic Characteristics and Responses of Tall Building Structures with Double Negative Stiffness Damped Outriggers

Sun, Feifei,Duan, Ningling,Wang, Meng,Yang, Jiaqi Council on Tall Building and Urban Habitat Korea 2021 International journal of high-rise buildings Vol.10 No.3

Dynamic characteristics of tall building structures with double negative stiffness damped outriggers (2NSDO) are parametrically studied using the theoretical formula. Compared with one negative stiffness damped outrigger (1NSDO), 2NSDO can achieve a similar maximal modal damping ratio with a smaller negative stiffness ratio. Besides, the 2NSDO can improve the maximum achievable damping ratio to about 30% with less consumption of an outrigger damping coefficient compared with the double conventional damped outriggers (2CDO). Besides, the responses of structures with 2NSDO under fluctuating wind load are investigated by time-history analysis. Numerical results show that the 2NSDO is effective in reducing structural acceleration under fluctuating wind load, being more efficient than 1NSDO.