http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Expression of Ion Channels in Perivascular Stem Cells derived from Human Umbilical Cords
Eunbi Kim,Won Sun Park,Seok-Ho Hong 한국발생생물학회 2017 발생과 생식 Vol.21 No.1
Potassium channels, the largest group of pore proteins, selectively regulate the flow of potassium (K+) ions across cell membranes. The activity and expression of K+ channels are critical for the maintenance of normal functions in vessels and neurons, and for the regulation of cell differentiation and maturation. However, their role and expression in stem cells have been poorly understood. In this study, we isolated perivascular stem cells (PVCs) from human umbilical cords and investigated the expression patterns of big-conductance Ca2+-activated K+ (BKCa) and voltage-dependent K+ (Kv) channels using the reverse transcription polymerase chain reaction. We also examined the effect of high glucose (HG, 25 mM) on expression levels of BKCa and Kv channels in PVCs. KCa1.1, KCaβ3, Kv1.3, Kv3.2, and Kv6.1 were detected in undifferentiated PVCs. In addition, HG treatment increased the amounts of BKCaβ3a, BKCaβ4, Kv1.3, Kv1.6, and Kv6.1 transcripts. These results suggested that ion channels may have important functions in the growth and differentiation of PVCs, which could be influenced by HG exposure.
Eunbi Jang,Thanh-Tam Ho,So-Young Park 한국화훼학회 2019 화훼연구 Vol.27 No.2
The aim of this study was to compare the two in vitro culture systems callus and adventitious root by investigating the biomass and phenolic compounds in calli and adventitious roots induced from four different explants (leaf, root, petal, and ovary) in Camellia japonica. The biomass of calli and adventitious roots was examined after 4 and 8 weeks of cultivation, respectively, and 22 phenolic compounds were analyzed using high-performance liquid chromatography (HPLC). The biomass of the ovary-derived calli (2.0 g・mass-1) was 1.5-fold that of the leaf-derived calli. The dry weight (DW) was highest in ovary-derived calli; however, the highest dry matter content was obtained from leaf-derived calli. Differences in the investigated characteristics depended on the callus origin. In adventitious roots, the highest biomass was achieved in the leaf-derived adventitious root system; its fresh weight was 2.3-fold (89 mg・ea-1) higher, and its DW was 1.8-fold (16 mg・ea-1) higher than those of ovary-derived adventitious root system. Active cell division was detected in petal-derived lines in both the calli and adventitious roots. Results of the HPLC analysis revealed that the total content of 22 phenolic compounds was highest in ovary-derived calli and ovary-derived adventitious roots. Our experiments confirmed that the calli and adventitious roots of C japonica have different cytological characteristics and bioactive compounds depending on the explant origin. In addition, callus culture was a more suitable system than adventitious root for producing phenolic compounds when the duration of the culture period and biomass were considered.
( Eunbi Ko ),( Kyoungmin Cho ),( Ji Hye Kim ),( Hyun-jung Shin ),( Byung-fhy Suh ),( Hye One Kim ),( Taeyoung Park ) 한국피부장벽학회 2023 한국피부장벽학회지 Vol.25 No.2
Customers have used various skin care and functional cosmetics to prevent their skin aging as well as improve current condition of the skin. It is important to analyzing and understanding their skin to select optimized skin care solutions. The aim of this study is to quantitatively analyze the effect of environment, lifestyle, and innate genes on the current skin condition. For this purpose, degree of hydration, sebum, wrinkles, melanin, dullness and redness in a highly controlled condition were collected in conjunction with a questionnaire survey analyzing their lifestyles and genetic data from about 3000 women. We classified participants based on the types of skin, using 6 kinds of index representing skin properties, questionnaire survey on lifestyle and genetic data with Gaussian Mixture Model and Decision Tree for classification model. Through this study, it was possible to divide the skin of Korean women into 12 clusters according to wrinkles, melanin, redness, dullness and oil/moisture balance with Gaussian Mixture Model. Also, we were able to identify a pattern in which each factor was correlated. Next, we tried to discover factors for predicting skin condition changes through correlation analysis with lifestyle, climate/environment, and innate genes that affect the current skin condition. Ultimate purpose of this study is to predict future skin condition using big data and AI technology. This knowledge will enable us to provide more proactive and personalized solutions not only for cosmetics but also for life care such as lifestyle, eating habits, and environmental response.
Development of a test method for the dynamic drapability of fabrics using reciprocating motion
Eunbi Yun,윤창상 한국의류학회 2023 Fashion and Textiles Vol.10 No.1
This study aimed to develop a method for measuring the dynamic drapability of fabrics using a reciprocating motion device that mimics the movement created by shaking a piece of fabric. Five types of fabrics were selected based on their drape coefficients and weights, and the lengths of fabrics were determined based on skirt length (50 cm) and dress length (100 cm). The width and speed of the reciprocating motion were considered as the experimental variables. Results of reciprocating motion-based fabric movement analysis revealed that the number of nodes, the position of the first node, amplitude, total length, and area are significant factors for measuring dynamic drapability. It was found that the longer fabrics had more nodes, enabling measurement of various types of fabrics. Furthermore, it was found that a wider reciprocation width under the same speed and a faster reciprocation speed with the same width favorably demonstrated dynamic drapability, resulting in proportionality between the reciprocating motion and the force transmitted to the fabric. Movement analysis of seven selected conditions and additional samples showed that the optimal condition was a 100-mm reciprocation width and a 150-rpm speed and that the first node’s position is the most significant factor for a dynamic drapability. To verify the proposed measurement method, measuring four pairs of fabrics with similar drape coefficients but different shapes showed that as fabric weight increased, the node moved downward, despite similar drape coefficient values. This confirms that the proposed method can overcome the limitations of the existing drape coefficient.
Development of digitized evaluation methods for fabric shrinkage and damage using image analysis
Eunbi Yun,Sungmin Kim,윤창상 한국의류학회 2023 Fashion and Textiles Vol.10 No.1
The aim of this study is to develop digitalized evaluation methods for fabric shrinkage and damage using image analysis. To develop the shrinkage assessment method, 81 grid dots (nine horizontal lines and nine vertical lines) are introduced into the test fabric. This allows the shrinkage to be verified based on the change in distance between neighboring dots, which not only presents the shrinkage in the warp and weft directions, but also the area shrinkage and skewness. The developed evaluation method yields a value that differs from the actual measured value by less than 1 mm, which confirms its significance. To develop a damage evaluation method using the Original Danish MA test fabric, the change in area caused by loosening threads in five holes is evaluated. The area change in the developed method and the number of loosened threads in the conventional method has a high correlation. Comparing the number of dots in the Poka-Dot test fabric before and after washing enables one to distinguish incorrectly created dots during the manufacturing process and to accurately measure the number of missing dots such that the result is similar to the actual measurement. The accuracy and reproducibility of the developed measurement method are confirmed using various verification such as error rate, washing cycle, and washing time. Digitizing the evaluation method can contribute to the digital transformation of apparel quality evaluation by reducing labor and errors between evaluators.