Antioxidant Composition of Strawberry Fruit in Relation to Storage Temperature and Relative Humidity

YoungJae Shin,Jung-A Ryu,Jackie F. Nock,Rui Hai Liu,Christopher B. Watkins 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Natural Frequencies of Euler-Bernoulli Beam with Open Cracks on Elastic Foundations

Youngjae Shin,Jonghak Yun,Kyeongyoun Seong,Jaeho Kim,Sunghwang Kang 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.4

A study of the natural vibrations of beam resting on elastic foundation with finite number of transverse open cracks is presented. Frequency equations are derived for beams with different end restraints. Euler-Bernoulli beam on Winkler foundation and Euler-Bernoulli beam on Pasternak foundation are investigated. The cracks are modeled by massless substitute spring. The effects of the crack location, size and its number and the foundation constants, on the natural frequencies of the beam, are investigated.

Development of high-throughput SNP markers from ‘Whangkeumbae’ and ‘Minibae’ pears using next generation sequencing

Youngjae Oh,Seolah Kim,Hyunsuk Shin,Jungyeon Won,Sam-Seok Kang,Yoon-Kyeong Kim,Cheol Choi,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Single nucleotide polymorphisms (SNPs) are the most frequent type among variations found in genomic regions and are valuable markers for genetic mapping, genetic diversity studies and association mapping in plants. There are three basic species known as Korean native which are Pyrus ussuriensis, P. pyrifolia, and P. fauriei. Genetic relationship among Korean pear cultivars compared with their parents was identified that they are closely related P. pyrifolia, P. ussuriensis and/or hybrids between two species. Lack of genetic resources, including molecular markers to study pears are very severe. Recently developed next generation sequencing (NGS) platforms offer opportunities for high-throughput and inexpensive genome sequencing and rapid marker development. The objective of this study was to develop polymorphic SNP markers in ‘Whangkeumbae’ and ‘Minibae’, which were chosen as the representative cultivars of P. pyrifolia and P. ussuriensis × pyrifolia in each among Korean pears, using genomic sequences generated by NGS technology. In this study, more than 18.6 Gbp and 15.8 Gbp sequences were obtained from NGS of ‘Whangkeumbae’ and ‘Minibae’, respectively. ‘Whangkeumbae’ and ‘Minibae’ contained 2,712,288 and 2,747,224 SNPs, respectively. In SNPs validations between ‘Whangkeumbae’ and ‘Minibae’, the number of polymorphic SNPs were 2,516,438 and non-polymorphic SNPs were 1,179,391. For HRM primer design, 2,125,479 HRM candidate primers were obtained from polymorphic SNPs and 343,731 SNP primers were developed. This study shows that the utility of NGS technology to discover efficiently a large number of SNPs and SNP primers can provide valuable information in the genome study of Pyrus spp.

High-throughput InDel marker development based on next generation sequencing in ‘Whangkeumbae’ and ‘Minibae’ pears

Youngjae Oh,Seolah Kim,Hyunsuk Shin,Jungyeon Won,Sam-Seok Kang,Yoon-Kyeong Kim,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Currently, the type of short insertions and deletions (InDels) polymorphisms are increasingly focused in genomic research. InDels have been known as a source of genetic markers that are widely spread across the genome. Genetic relationship among Korean pear cultivars compared with their parents was also identified that they are closely related P. pyrifolia, P. ussuriensis and/or hybrids between two species. Lack of genetic resources including molecular markers has made it difficult to study pears severely. Recently developed next generation sequencing (NGS) platforms offer opportunities for high-throughput and inexpensive genome sequencing and rapid marker development. The main goal of this study was to develop polymorphic InDel markers in ‘Whangkeumbae’ and ‘Minibae’, which were chosen as the representative cultivars of P. pyrifolia and P. ussuriensis × pyrifolia in each among Korean pears using genomic sequences generated by NGS technology. In this study, more than 18.6 Gbp and 15.8 Gbp sequences were obtained from NGS of ‘Whangkeumbae’ and ‘Minibae’, respectively. ‘Whangkeumbae’ contained 197,210 InDels and 197,272 InDels in ‘Minibae’. In InDels validations between ‘Whangkeumbae’ and ‘Minibae’, the number of polymorphic InDels were 149,338 and non-polymorphic InDels were 122,572. For InDel primer set designing, 11,308 of primers were designed from polymorphic InDels and 10,919 of InDel primers were recommended. The study shows that the utility of NGS technology to design amount of efficient InDels and the developed InDel primers will be used for genetic mapping, breeding by marker assisted selection (MAS) and QTL mapping of Korea native pear as well as further genetic studies.

Mono-component multiparameter acoustic full waveform inversion in vertically transverse isotropic media using converted vector wavefields

Shin, Youngjae,Oh, Ju-Won,Kim, Shinwoong,Min, Dong-Joo Elsevier 2019 Journal of applied geophysics Vol.170 No.-

<P><B>Abstract</B></P> <P>In multiparameter full-waveform inversion (FWI) with acoustic-approximation for vertically transverse isotropic (VTI) media, it is important to choose an appropriate modeling technique for computational efficiency and numerical stability. In addition, because the gradient is determined by the modeling algorithm used in the FWI process, we need to examine if the modeling algorithm yields a gradient similar to that obtained from the elastic scattering theory. The decomposed equation with mono-component has been proposed because it is computationally efficient and does not generate shear-wave artifacts. However, the decomposed equation has the limitation that its numerical scattering potentials for the perturbations of anisotropic parameters do not properly simulate the theoretical elastic scattering potentials. To overcome the limitation, we propose using the vector virtual sources to compute the gradient in the FWI algorithm by converting the mono-component (pressure) source and receiver wavefields into the two-component particle displacements using the equation of motion on the staggered grids. Unlike the conventional method, our approach properly simulates PP radiation patterns for anisotropic parameters obtained under the elastic assumption, while retaining computational efficiency achieved by using the mono-component. Numerical examples show that anisotropic parameters are updated in the correct gradient direction by our method in the FWI process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The acoustic anisotropic FWI using the pseudo-analytic (mono-component) acoustic wave equation has a difficulty to describe radiation patterns of partial derivative wavefields compare to the elastic scattering theory. </LI> <LI> We propose an alternative approach, which calculate the gradient direction with the vector wavefields converted from the mono-component pressure wavefields. </LI> <LI> Comparing the scattering pattern of the partial derivative wavefields and sensitivity kernels, we show the vector-based gradient direction from our proposed approach describe the behavior of the partial derivative wavefields in multi-parameter anisotropic FWI. </LI> <LI> We verify the accuracy and feasibility of our approach to the simple syncline and the modified Marmosui-2 models. </LI> </UL> </P>

Proline accumulation and related gene expression in response to higher temperatures during deacclimation in peach shoot tissues

Hyunsuk Shin,Sewon Oh,Keumsun Kim,Youngjae Oh,Jungyeon Won,Hyeondae Han,Daeil Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Proline (Pro) accumulation is a common physiological reaction in response to abiotic stresses in many plants. Accumulation of Pro is believed to play the important role in protecting cellular components from dehydrating effects due to such stresses. The study was performed to investigate the relationship between cold hardiness and Pro content or expression of related genes in peach cultivars during a constant experimental deacclimation. Changes in cold hardiness were determined using electrolyte leakage method in the shoots of 10 peach cultivars (Prunus persica ‘Aikawanakajima’, ‘Chiyomaru’, ‘Daewol’, ‘Janghowon Hwangdo’, ‘Kiraranokiwami’, ‘Mihong’, ‘Misshong’, ‘Soomee’, ‘Suhong’, and ‘Sun Gold’). Pro content was analyzed using the ninhydrin method and related gene expressions were examined using quantitative real-time RT-PCR. While cold hardiness of 10 peach cultivars decreased, Pro contents of those increased during the deacclimation. Notably, at the same time, expression of P5CS (Δ1-pyrroline-5-carboxylatesynthase) decreased in 10 peach cultivars, whereas expressions of P5CR (Δ1-pyrroline-5-carboxylatereductase) and OAT (ornithine-δ-aminotransferase) increased. Our results demonstrate that Pro responds positively to higher temperature in the shoots of 10 peach cultivars and expression of both P5CS and P5CR genes could show contrasting patterns during the deacclimation. Furthermore, our results suggest that ornithine pathway, which has been suggested to be important during seedling development, could serve as an alternative pathway in Pro synthesis process during the deacclimation in peach.

Cold Hardiness, Proline Content, and Expression of P5CS and P5CR Genes among Peach Cultivars in Field Conditions

Hyunsuk Shin,Sewon Oh,Keumsun Kim,Youngjae Oh,Seolah Kim,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Changes in cold hardiness were confirmed with relative electrolyte leakage (REL) method in the shoots of two peach cultivars (Prunus persica Janghowon Hwangdo and Odoroki) during cold acclimation and deacclimation. Changes in proline (Pro) content and related gene expressions were also analyzed. Particularly, transcript accumulations of P5CS and P5CR were examined using quantitative real-time RT-PCR. REL in the shoots of two peach cultivars was significantly different during the entire experimental period. Cold hardiness of two cultivars increased gradually to December 2012, and then decreased to April 2013, whereas Pro contents of ‘Janghowon Hwangdo’ and ‘Odoroki’ were reduced from the beginning of the experiment to February 2012 and then increased in the spring. Interestingly, P5CS gene encoding an enzyme, which catalyzes conversion from glutamic acid (Glu) into glutamic-γ-semialdehyde (GSA) in the first step of Pro pathway, showed the contrasting patterns with Pro contents of two cultivars. On the other hand, P5CR gene encoding an enzyme, which catalyzes conversion from Δ1-pyrroline-5-carboxylate(P5C)intoPro in the final step of Pro pathway, showed the similar patterns to Pro contents in two cultivars. Our results demonstrate that Pro responds negatively to low temperatures in the shoot of different peach cultivars, including the supplemental 10 peach cultivars, and expression of both P5CS and P5CR genes could show contrasting patterns from each other. Our results suggest that identification of both P5CS and P5CR genes are required necessarily for accurate analysis of Pro biosynthesis because Pro accumulation is affected more by expression of P5CR gene.

고등학교 졸업학력 검정고시 ‘과학’ 문항의 내용 요소와 인지적 수준 분석

신영재 ( Youngjae Shin ),강경희 ( Kyunghee Kang ) 인하대학교 교육연구소 2021 교육문화연구 Vol.27 No.6

이 연구는 고등학교 졸업학력 검정고시 과학의 출제 현황을 분석하고, 그를 토대로 개선 방안을 고찰하는 것이다. 이를 위해 2014년부터 2020년까지의 고졸 검정고시 과학문항을 고등학교 과학교육과정의 내용 요소와 인지적 수준에 따라 분석하였다. 해당 기간의 고졸 검정고시는 2009 개정 교육과정에 근거해 출제되었으므로 2009 개정 교육과정 10학년 과학의 내용체계에 따라 분석하였다. 분석 결과 ‘태양계와 지구’ 단원이 가장 많은 출제 비중을 차지하였고. 다음으로는 ‘생명의 진화’가 많이 출제되었다. 이에 비해 ‘우주의 기원과 진화’ 단원은 가장 적게 출제된 것으로 나타나 각 단원별로 고르게 출제가 이루어지지 않은 것으로 나타났다. 또한 검정고시 출제기준에서 제시하고 있는 인지적 영역인 지식, 이해, 적용 수준에 따라 분석한 결과 지식 수준 38.0%, 이해 수준 50.3%, 적용 수준 11.7%로 나타났다. 이와 같은 결과는 지식과 이해 수준에 비해 적용 수준의 문항 비율이 너무 낮음을 의미한다. 그러므로 검정고시 과학 문항 출제를 위해 교육과정 내용 요소와 인지적 수준에 대한 분석을 실시하고, 그를 토대로 균형있는 출제가 이루어져야 할 것이다. 이 연구의 결과는 향후 고졸 검정고시 과학 문항 출제의 방향성과 구체적인 출제 기준에 대한 기초 자료를 제공할 수 있을 것으로 기대한다. The aim of this study was to analyze and improve the science curriculum for the high school graduate equivalency test (HSGET). The science questions of HSGET from 2014 to 2020 were analyzed according to the content and cognitive level of the high school science curriculum Since the examinations for that period were based on the 2009 revised curriculum, they were analyzed according to the 10th grade science content system of the said curriculum. The analysis revealed that the unit on solar system and earth accounted for the largest proportion of questions asked, followed by ‘the evolution of life’. Conversely, the section ‘the origin and evolution of the universe’ had the least number of questions, indicating that the questions were not evenly distributed in each unit. Additionally, results of the analysis found only 38.0% of knowledge level, 50.3% of understanding level, and 11.7% of application level compared to cognitive levels of knowledge, comprehension, and application suggested in the qualification examination criteria. This means that the proportion of questions at the application level is fairly low compared to the level of knowledge and understanding. Therefore, it is necessary to analyze the content of the curriculum and cognitive level of science questions in HSGET and create a balanced question paper based on the analysis. The results of this study are expected to provide basic data on the direction and specific criteria for science questions in HSGET in the future.

고속 공작기계 주축용 앵귤러 콘택트 볼 베어링의 냉각효과

신영재(Youngjae Shin),이후상(Husang Lee),김태형(Taehyung Kim),박보선(Bosun Park) 한국정밀공학회 1997 한국정밀공학회 학술발표대회 논문집 Vol.1997 No.5월

미분구적법에 의한 탄성지반상의 열림 균열을 가진 EUler-Bernoulli보의 진동 해석

신영재(Youngjae Shin),황기섭(Kisup Hwang),윤종학(Jonhak Yun),노엘(N. M. Dioyan) 한국자동차공학회 2003 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

In this paper, Generalized Differential Quadrature Method is applied vibration analysis of Euler-Bernoulli beam with open cracks on elastic foundation. The natural frequencies of Euler-Bernoulli beam with open cracks on elastic foundation were obtained and analyzed for various boundary conditions by using the Generalized Differential Quadrature Method. The concepts of Generalized Differential Quadrature Method were briefly introduced. The cracks are modeled by massless substitute spring. The effects of the crack location, size and the foundation constants, on the natural frequencies of the beam, are investigated.