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Eunbi Jang,Thanh-Tam Ho,So-Young Park 한국화훼학회 2019 화훼연구 Vol.27 No.2
The aim of this study was to compare the two in vitro culture systems callus and adventitious root by investigating the biomass and phenolic compounds in calli and adventitious roots induced from four different explants (leaf, root, petal, and ovary) in Camellia japonica. The biomass of calli and adventitious roots was examined after 4 and 8 weeks of cultivation, respectively, and 22 phenolic compounds were analyzed using high-performance liquid chromatography (HPLC). The biomass of the ovary-derived calli (2.0 g・mass-1) was 1.5-fold that of the leaf-derived calli. The dry weight (DW) was highest in ovary-derived calli; however, the highest dry matter content was obtained from leaf-derived calli. Differences in the investigated characteristics depended on the callus origin. In adventitious roots, the highest biomass was achieved in the leaf-derived adventitious root system; its fresh weight was 2.3-fold (89 mg・ea-1) higher, and its DW was 1.8-fold (16 mg・ea-1) higher than those of ovary-derived adventitious root system. Active cell division was detected in petal-derived lines in both the calli and adventitious roots. Results of the HPLC analysis revealed that the total content of 22 phenolic compounds was highest in ovary-derived calli and ovary-derived adventitious roots. Our experiments confirmed that the calli and adventitious roots of C japonica have different cytological characteristics and bioactive compounds depending on the explant origin. In addition, callus culture was a more suitable system than adventitious root for producing phenolic compounds when the duration of the culture period and biomass were considered.
Jang, Ik-Soon,Jo, Eunbi,Park, Soo Jung,Baek, Su Jeong,Hwang, In-Hu,Kang, Hyun Mi,Lee, Je-Ho,Kwon, Joseph,Son, Junik,Kwon, Ho Jeong,Choi, Jong-Soon The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Background: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. Methods: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. Results: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. Conclusion: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.
장현석 ( Hyunseok Jang ),홍명기 ( Myeonggi Hong ),이경아 ( Gyeonga Lee ),조은비 ( Eunbi Cho ) 대한범죄학회 2021 한국범죄학 Vol.15 No.1
현재 서구사회의 범죄 연구는 지리적 측면의 중요성에 초점을 맞추어 이와 관련된 실증적 연구들이 많이 이루어지고 있다. 그러나 우리나라에서는 자료의 수집의 어려움으로 인하여 범죄와 공간의 관계 그리고 지역의 환경적 특징의 영향에 대한 논의가 매우 제한적이다. 따라서 본 연구는 탐색적 수준에서 서울시의 행정동 수준의 범죄 수준을 측정하고, 이를 이용하여 서울특별시의 범죄의 지리적 분포를 지도를 이용하여 제시하는 것을 목표로 하였다. 행정안전부가 제공한 생활안전 지도를 바탕으로 서울지역 행정동 단위의 범죄 데이터를 수집하고, 공간분석을 통해 8가지 범죄 유형(살인, 강도, 방화, 성폭력, 폭력, 절도, 마약, 도박)에 대하여 서울시의 범죄 분포를 살펴보았다. 분석 결과 지도상으로 제시한 범죄발생등급의 분포의 경우 각 범죄마다 차이가 존재하였으며, 사회해체이론의 집중된 불이익지표의 분포와 상이하였다. 또한 범죄의 유형에 따라 핫스팟과 콜드스팟이 군집된 위치가 변화함을 확인할 수 있었다. 특히, 서초구와 강남구의 경우 북쪽에 위치한 행정동은 살인과 방화를 제외한 범죄에 대해 핫스팟을 보였으나, 남쪽에 위치한 행정동은 마약과 도박을 제외한 범죄에 대해 콜드스팟을 보이는 등 같은 시군구이더라도 범죄의 유형에 따라 그 분포에 차이를 보이는 것으로 나타났다. 마지막으로, 이러한 결과들을 통해 시사점과 한계점을 논하였다. Currently, crime research in Western society focuses on the importance of the geographic aspect, and there are many empirical studies related to this. However, in Korea, due to the difficulty of collecting data, studies on the relationship between crime and space and the influence of regional environmental characteristics are very limited. Therefore, with the purpose of exploratory research, this study aimed to measure crime level at administrative dong level in Seoul. Based on the life safety map provided by the Ministry of Public Administration and Security, crime data of Seoul have been collected. And this study tried to analyze eight types of crimes (murder, robbery, arson, sexual violence, violence, theft, drugs, and gambling) through spatial analysis. As a result of the analysis, in the case of the distribution of crime incidence level, there were differences among crime categories, and the distribution of crime incidence level showed different aspect from the distribution of the concentrated disadvantage indicators of the social disorganization theory. In addition, this study found that the location of hot spots and cold spots clustered differently according to the type of crime. In particular, in Seocho-gu and Gangnam-gu, administrative dongs located in the north showed hot spots for crimes excluding murder and arson, while administrative dongs located in the south showed cold spots for crimes excluding drugs and gambling. It was found that there was a difference in its distribution accordingly. Finally, through these results, the implications and limitations were discussed.
Affecting factors on the decline of serum antimullerian hormone after ovarian cystectomy
( Bomin Kim ),( Eunbi Jang ),( Nae Ri Kim ),( Eun Jung Yang ),( Seung-hyuk Shim ),( Sun Joo Lee ),( Tae Jin Kim ),( Kyeong A So ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-
Objective: To investigate the affecting factors on the decline of serum antimullerian hormone (AMH) in the reproductive aged women with ovarian cystectomy. Methods: This retrospective study included reproductive aged women who underwent ovarian cystectomy for benign disease from January 2019 to March 2020 at the Konkuk University Medical Center. Medical charts were reviewed about clinical characteristics, preoperative/postoperative serum AMH levels, operative records, and histopathologic results. Results: During the study period, 40 patients were included. The mean age of the patients was 33.3 years old. All patients except one underwent minimally invasive surgery including robotic and laparoscopic ovarian cystectomy. Twenty-six patients found to be endometriotic cyst and 14 patients were non-endometriotic cyst. During the surgery, the method of hemostasis after ovarian cystectomy was performed mainly by suture (97.5%) instead of electrocoagulation (2.5%). The rate of decline of serum AMH level showed statistically significant associations with age (r=0.325, p=0.040), duration of surgery (r=0.424, p=0.006), endometriosis (p<0.0001), bilaterality of ovarian cyst (p=0.014), and adhesiolysis during surgery (p=0.012). A logistic regression analysis was performed for affecting factors on the postoperative AMH decline (≥ 50%) compared to preoperative AMH level. Endometriosis was the only statistically associated with postoperative AMH decline (Odd ratio 7.10; 95% confidential interval, 1.06-47.76; p= 0.044). Conclusion: The rate of decline of serum AMH level is significant association with age, duration of surgery, endometriosis, bilaterality of ovarian cyst, and adhesiolysis during surgery. In particular, endometriosis appears to be the main cause of AMH decline.