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Deok Ho Kwon,Keon mook Seong,Kyong Sup Yoon,J. Marshall Clark,Won Ja Lee,Yong Joon Ahn,Si Hyeock Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05
A quantitative sequencing (QS) protocol that detects the frequencies of sodium channel mutations (M815I, T917I and L920F) responsible for knockdown resistance in permethrin-resistant head lice was tested as a population genotyping method. Genomic DNA fragments of the sodium channel α-subunit gene that encompass the three mutation sites were PCR-amplified from individual head lice with either resistant or susceptible genotypes, and combined together in various ratios to generate standard DNA template mixtures for QS. Following sequencing, the signal ratios between resistant and susceptible nucleotides were calculated and plotted against the corresponding resistance allele frequencies. Quadratic regression coefficients of the plots were close to 1, demonstrating that QS is highly reliable for the prediction of resistance allele frequencies. Prediction of resistance allele frequencies by QS in several globally collected lice samples including 12 Korean lice populations suggested that permethrin resistance varied substantially amongst different geographical regions. Three local populations of Korean lice were determined to have 9.8-36.7% resistance allele frequencies, indicating that an urgent resistance management is needed. QS should serve as a preliminary resistance monitoring tool for proper management strategies by allowing early resistance detection.
Yang, Deok-Hwan,Kim, Mi-Hyun,Lee, Youn-Kyung,Hong, Cheol Yi,Lee, Hyun Ju,Nguyen-Pham, Thanh-Nhan,Bae, Soo Young,Ahn, Jae-Sook,Kim, Yeo-Kyeoung,Chung, Ik-Joo,Kim, Hyeoung-Joon,Kalinski, Pawel,Lee, Je-J Springer International 2011 Annals of hematology Vol.90 No.12
<P>For wide application of a dendritic cell (DC) vaccination in myeloma patients, easily available tumor antigens should be developed. We investigated the feasibility of cellular immunotherapy using autologous alpha-type 1-polarized dendritic cells (관DC1s) loaded with apoptotic allogeneic myeloma cells, which could generate myeloma-specific cytotoxic T lymphocytes (CTLs) against autologous myeloma cells in myeloma patients. Monocyte-derived DCs were matured by adding the 관DC1-polarizing cocktail (TNF관/IL-1관/IFN-관/IFN-관/poly-I:C) and loaded with apoptotic allogeneic CD138(+) myeloma cells from other patients with matched monoclonal immunoglobulins as a tumor antigen. There were no differences in the phenotypic expression between 관DC1s loaded with apoptotic autologous and allogeneic myeloma cells. Autologous 관DC1s effectively took up apoptotic allogeneic myeloma cells from other patients with matched subtype. Myeloma-specific CTLs against autologous target cells were successfully induced by 관DC1s loaded with allogeneic tumor antigen. The cross-presentation of apoptotic allogeneic myeloma cells to 관DC1s could generate CTL responses between myeloma patients with individual matched monoclonal immunoglobulins. There was no difference in CTL responses between 관DC1s loaded with autologous tumor antigen and allogeneic tumor antigen against targeting patient's myeloma cells. Our data indicate that autologous DCs loaded with allogeneic myeloma cells with matched immunoglobulin can generate potent myeloma-specific CTL responses against autologous myeloma cells and can be a highly feasible and effective method for cellular immunotherapy in myeloma patients.</P>
이덕기(Lee, Deok-Ki),이의준(Lee, E. J.),최상진(Choi, Sang-Jin),박수억(Park, Soo-Uk),이상설(Lee, Sang-Sul) 한국신재생에너지학회 2005 신재생에너지 Vol.1 No.2
New & renewable energy technology development has been carrying out in 11 different areas so far. Many technologies are being applied to the required areas according to its own characteristics. Before launching the development, these technologies have been evaluated by their commercial effect and contribution to energy supply. In this paper, the impact factors enhancing the distribution of renewable energy were developed to select appropriate diffusion regulations for each technology area. In addition, AHP methodology was introduced to investigate priorities of the regulations along with weights of impact factors
Electrodynamic properties of the semimetallic Dirac materialSrMnBi2: Two-carrier-model analysis
Park, H. J.,Park, Byung Cheol,Lee, Min-Cheol,Jeong, D. W.,Park, Joonbum,Kim, Jun Sung,Ji, Hyo Seok,Shim, J. H.,Kim, K. W.,Moon, S. J.,Kim, Hyeong-Do,Cho, Deok-Yong,Noh, T. W. American Physical Society 2017 Physical Review B Vol.96 No.15
<P>The electrodynamics of free carriers in the semimetallic Dirac material SrMnBi2 was investigated using optical spectroscopy and first-principles calculations. Using a two-carrier-model analysis, the total free-carrier response was successfully decomposed into individual contributions from Dirac fermions and non-Dirac free carriers. Possible roles of chiral pseudospin, spin-orbit interaction (SOI), antiferromagnetism, and electron-phonon (e-ph) coupling in the Dirac fermion transport were also addressed. The Dirac fermions possess a low scattering rate of similar to 10 meV at low temperature and thereby experience coherent transport. However, at high temperatures, we observed that the Dirac fermion transport becomes significantly incoherent, possibly due to strong e-ph interactions. The SOI-induced gap and antiferromagnetism play minor roles in the electrodynamics of the free carriers in SrMnBi2. We also observed a seemingly optical-gap-like feature near 120 meV, which emerges at low temperatures but becomes filled in with increasing temperature. This gap-filling phenomenon is ascribed to phonon-assisted indirect transitions promoted at high temperatures.</P>