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ISOLATION OF THE SMALLEST RNA POLYMERASE Ⅱ SUBUNIT GENE, BRP10, FROM BRASSICA NAPUS
Kwak, June-Myoung,Hong, Suk-Whan,Nam, Hong-Gil Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
We have isolated the smallest RNA polymerase(RNAP)Ⅱ subunit gene, brp10, which is highly homologous to a yeast RNAP Ⅱ subunit RPB10, an essential component of RNAPⅡ for yeast cell viability, while conducting the partial sequencing of random root cDNA clones of Brassica napus. The brp10 has an open reading frame of 216bp encoding 71 amino acid residues and showed 68% identity at the peptide level and 64% identity at the nucleic acid level to the counterpart. In addition, brp10 contains a potential zinc-binding motif conserved in several subunits of RNA polymerases. Northern blot analysis revealed that transcripts of 0.8kbp are expressed in all vegetative tissues. This is the first report on RNAPⅡ subunit RPB10 homolog from higher eukaryotes.
MOLECULAR CLONING AND CHARACTERIZATION OF A G PROTEIN β SUBUNIT HOMOLOG, BGB1, FROM BRASSICA NAPUS
Kwak, June-Myoung,Kim, Sun-A,O, Sung-Aeong,Byoun, Chang-Hyoun,Nam, Hong-Gil Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
We have isolated a cDNA clone, named bgb1, encoding a G protein β subunit homolog from Brassica napus. The predicted 327-residue bgbl, protein (Mr 35,725) shows relatively low homology (20% identity) to mammalian Gβ subunits, but it has a strong homology (60% identity) to RACK1, which is an intracellular receptor for protein kinase C and has a homology to the G protein β subunit (Ron et al., 1994). In addition, bgbl is composed of seven segments of WD-40 repeats that is a well-known characteristic of Gβ subunits. Genomic Southern blot analysis in B. napus and Arabidopsis thaliana revealed that bgbl and its closely related sequences consist of a multigene family in both plants. Approximately 1.4 kilobase transcripts are observed in all organs of B. napus examined by Northern blot analysis. Wound- and touch-treatments do not affect the expression pattern of bgbl in B. napus, but 5 days-dark treatment reduces the bgbl-homologous transcripts in datached leaves of Arabidopsis.