Comet Assay의 이해와 응용

Cuong, Dang Van,김충희,김나리,김의용,한진 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Single cell gel(SCG) electrophoresis or 'Comet assay' is a rapid and very sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. This assay has critically important applications in fields of toxicology ranging from aging and clinical investigations to genetic toxicology and molecular epidemiology. Since the introduction of the alkaline Comet assay in 1988, a number of advancements have greatly increased the flexibility and utility of this technique for detecting various forms of DNA damage (e.g., single- and double-strand breaks, oxidative DNA base damage, and DNA-DNA/DNA-protein/DNA-Drug crosslinking) and DNA repair in virtually any eukaryotic cell.

Approaches to e-Health : Principles and Prospects

Cuong, Dang Van,Kim, Nari,Kim, Euiyong,Han, Jin 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

컴퓨터, 네트워크 스토리지 솔루션 그리고 원격 전자통신 기술을 이용한 원격의료의 발전을 통한 의료서비스의 질적 개선은 선진국에서는 이미 상당한 수준으로 진행되고 있다. 의료비용절감, 첨단의료기술의 광범위한 적용, 의료정책 변화에 대한 능동적 대처 그리고 의료사고의 위험성 해소 등 좀 더 나은 의료서비스를 위한 사회경제적 필요성은 어느 때보다 폭 넓은 공감대를 형성하고 있다. 전자의료기록, 디지털 MRI 및 X선, 그리고 환자 전자우편 서비스 등은 최근 발전하고 있는 첨단 애플리케이션의 일부에 불과하다. 원격의료는 복잡한 정보 인프라와 법적 지원을 요구하는 최첨단의 전자통신 시스템의 구축을 바탕으로 한 의료 서비스이다. 이를 통한 환자 및 전체 의료업무에 대한 정보를 통합, 관리 및 공유는 보다 신속하고 높은 수준의 의료서비스를 가능하게 할 것이다. 인제대학교 의과대학 및 부속 병원 사이의 효율적이고 모범적인 원격진료의 모델을 개발하기 위한 기본 단계로서 원격진료의 원리와 앞으로 전망에 대해 알아보고자 한다. Computing, networking, and telecommunications support for health care in industrially developed countries have become essential day-to-day services. This support has begun to have a significant impact on the geographic coverage, methods, economy, and efficiency of care, and is becoming increasingly important, both economically and socially. Applications are increasing with the rapid development of the informatics revolution, e-Health is healthcare service support based on the telecommunication a high-level infrastructure and legal support. This paper discusses some of the principles and prospects of e-health in order to draw a complete picture of a model system, which will help us to understand and plan a network of hospitals and schools.

Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Dang Van Cuong,김나리,김의용,이영석,김현주,강성현,허대영,주현,박영식,홍영근,이상경,정주명,석대현,한진 대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.4

Nitric oxide-cGMP-protein kinase G signaling pathway induces anoxic preconditioning through activation of ATP-sensitive K+ channels in rat hearts

Cuong, Dang Van,Kim, Nari,Youm, Jae Boum,Joo, Hyun,Warda, Mohamad,Lee, Jae-Wha,Park, Won Sun,Kim, Taeho,Kang, Sunghyun,Kim, Hyungkyu,Han, Jin American Physiological Society 2006 American journal of physiology, Heart and circulat Vol.290 No.5

<P> Nitric oxide (NO) plays an important role in anoxic preconditioning to protect the heart against ischemia-reperfusion injuries. The present work was performed to study better the NO-cGMP-protein kinase G (PKG) signaling pathway in the activation of both sarcolemmal and mitochondrial ATP-sensitive K<SUP>+</SUP> (KATP) channels during anoxic preconditioning (APC) and final influence on reducing anoxia-reperfusion (A/R)-induced cardiac damage in rat hearts. The upstream regulating elements controlling NO-cGMP-PKG signal-induced KATP channel opening that leads to cardioprotection were investigated. The involvement of both inducible and endothelial NO synthases (iNOS and eNOS) in the progression of this signaling pathway was followed. Final cellular outcomes of ischemia-induced injury after different preconditioning in the form of lactate dehydrogenase release, DNA strand breaks, and malondialdehyde formation as indexes of cell injury and lipid peroxidation, respectively, were investigated. The lactate dehydrogenase and malondialdehyde values decreased in the groups that underwent preconditioning periods with specific mitochondrial KATP channels opener diazoxide (100 μM), nonspecific mitochondrial KATP channels opener pinacidil (50 μM), S-nitroso- N-acetylpenicillamine (SNAP, 300 μM), or β-phenyl-1, N<SUP>2</SUP>-etheno-8-bromoguanosine-3′,5′-cyclicmonophosphorothioate, Sp-isomer (10 μM) before the A/R period. Preconditioning with SNAP significantly reduced the DNA damage. The effect was blocked by glibenclamide (50 μM), 5-hydroxydecanoate (100 μM), N<SUP>G</SUP>-nitro-l-arginine methyl ester (200 μM), and β-phenyl-1, N<SUP>2</SUP>-etheno-8-bromoguanosine-3′,5′-cyclic monophosphorothioate, Rp-isomer (1 μM). The results suggest iNOS, rather than eNOS, as the major contributing NO synthase during APC treatment. Moreover, the PKG shows priority over NO as the upstream regulator of NO-cGMP-PKG signal-induced KATP channel opening that leads to cardioprotection during APC treatment. </P>

Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes

Cuong, Dang Van,Kim, Na-Ri,Kim, Eui-Yong,Lee, Young-Suk,Kim, Hyun-Ju,Kang, Sung-Hyun,Hur, Dae-Young,Joo, Hyun,Park, Young-Shik,Hong, Yong-Geun,Lee, Sang-Kyung,Chung, Joon-Yong,Seog, Dae-Hyun,Han, Jin The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.4

Mitochondrial ATP-sensitive potassium $(mitoK_{ATP})$ channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of $mitoK_{ATP}$ channels remains unclear. In this study, we investigated the subunit composition of $mitoK_{ATP}$ channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while $mitoK_{ATP}$ channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of $mitoK_{ATP}$ channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands $({\sim}44,\;{\sim}46,\;and\;{\sim}30\;kDa)$ were detected, and a specific ${\sim}46-kDa$ immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of $mitoK_{ATP}$ channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.

Linkage Between Exchange Rate and Stock Prices: Evidence from Vietnam

DANG, Van Cuong,LE, Thi Lanh,NGUYEN, Quang Khai,TRAN, Duc Quang Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.12

The study investigates the asymmetric effect of exchange rate changes on stock prices in Vietnam. We use the nonlinear autoregressive-distributed lag (ARDL) analysis for monthly data from 2001:01 to 2018:05, based on VN-Index stock price collected from Ho Chi Minh Stock Exchange (HOSE); the nominal exchange rate is separated into currency depreciation and appreciation through a partial sum decomposition process. Asymmetry is estimated both in the long-run relationship and the short-run error correction mechanism. The research results show that the effect of exchange rate changes on stock prices is asymmetrical, both in the short run and in long run. Accordingly, the stock prices react to different levels to depreciation and appreciation. However, the currency appreciation affects a stronger transmission of stock prices when compared to the long-run currency depreciation. In the absence of asymmetry, the exchange rate only has a short-run impact on stock prices. This implies a symmetrical assumption that underestimates the impact of exchange rate changes on stock prices in Vietnam. This study points to an important implication for regulators in Vietnam. They should consider the relationship between exchange rate changes and stock prices in both the long run and the short run to manage the stock and foreign exchange market.

General considerations and a comparison of environmental effects on students at two campuses of Inje University

Dang, Van Cuong,Nguyen, Quang Minh,Dinh, Minh Hoa,Kim, Euiyong,Kim, Nari,Han, Jin 인제대학교 백병원 2003 仁濟醫學 Vol.24 No.2

목적 및 방법 : 다양한 환경의 변화들은 사람의 건강에 직, 간접적으로 영향을 미쳐, 작업이나 학업의 능률을 저하시킬 수 있다 이러한 관점에서 정신적. 육체적 건강은 매우 중요하며. 이들은 일반적인 좋은 건강과 경제적인 성취를 가능하게 한다. 실내환경은 일 또는 학업의 능률을 증진시키는데 중요한 요인 중의 하나이다. 이와 관련하여 인제대학교 김해캠퍼스와 부산캠퍼스의 남녀 학생들의 의견을 설문조사를 통해 조사하였다. 결과 : 김해캠퍼스 학생의 72.3%는 자신들이 건강하다고 느끼고 있었으며, 반면 이 부산캠퍼스의 학생은 47.3%만이 건강하다고 응답하였다. 부산캠퍼스에서 응답 학생의 86.0%의 학생들은 그들의 학업환경이 열악하다고 답하였으며, 가장 심각한 문제는 소음과 공기오염 등이었다. 결론 : 이러한 학업환경의 차이에 대한 결과는 쾌적한 교내생활 환경을 조성하기 위한 기초자료가 될 것으로 사료된다.

Mitochondrial calcium uniporter inhibition attenuates mouse bone marrow-derived mast cell degranulation induced by beta-1,3-glucan

Dang, Van Cuong,Kim, Hyoung Kyu,Marquez, Jubert,Kim, Nari,Ko, Kyung Soo,Rhee, Byoung Doo,Han, Jin The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.2

Mast cells are primary mediators of allergic inflammation. Beta-1,3-glucan (BG) protects against infection and shock by activating immune cells. Activation of the BG receptor induces an increase in intracellular $Ca^{2+}$, which may induce exocytosis. However, little is known about the precise mechanisms underlying BG activation of immune cells and the possible role of mitochondria in this process. The present study examined whether BG induced mast cell degranulation, and evaluated the role of calcium transients during mast cell activation. Our investigation focused on the role of the mitochondrial calcium uniporter (MCU) in BG-induced degranulation. Black mouse (C57) bone marrow-derived mast cells were stimulated with $0.5{\mu}g/ml$ BG, $100{\mu}g/ml$ peptidoglycan (PGN), or $10{\mu}M$ A23187 (calcium ionophore), and dynamic changes in cytosolic and mitochondrial calcium and membrane potential were monitored. BG-induced mast cell degranulation occurred in a time-dependent manner, and was significantly reduced under calcium-free conditions. Ruthenium red, a mitochondrial $Ca^{2+}$ uniporter blocker, significantly reduced mast cell degranulation induced by BG, PGN, and A23187. These results suggest that the mitochondrial $Ca^{2+}$ uniporter has an important regulatory role in BG-induced mast cell degranulation.

Strategy To Study Ischemic Preconditioning : Role of ATP-Sensitive K+ Channels in the rat heart 쥐 심장에서 ATP-의존성 칼륨-통로의 역할을 중심으로

Cuong, Dang Van,Kim, Nari,Kim, Euiyong,Han, Jin 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

허혈전처치는 짧은 시간의 허혈과 재관류를 미리 반복해서 실시하면 다음에 오는 장시간의 허혈 및 재관류에 의한 심장기능의 저하, 부정맥의 발생 및 심근세포의 손상이 현저히 완하되는 현상이다. 내인성 심장보호기전으로서의 허혈전처치는 여러 가지의 가능한 작용기전들이 제시되어왔다. 지금까지 알려진 가능한 기전으로Reduced energy demand, mito-chondrial ATPase inhibitor protein, free radicals, stress proteins, prostacyclin, nitricoxide(NO), adenosine, glycolytic flux, ATP-sensitive potassium channel(K_(ATP)-통로)등이 알려져 왔다. 이 중에서 K_(ATP)-통로는 허혈전처치의 end effector로서 중요한 역할을 담당하는 것으로 알려지고 있다. 하지만 허혈전처치의 심장보호효과의 중요 기전으로서 심장 세포막 K_(ATP)-통로의 상대적인 중요성에 대해서는 아직도 논란의 여지가 많은 실정이다. Single-cell gel electrophoresis 방법을 이용하여 허혈전처치의 심장보호효과와 심장 세포막 K_(ATP)-통로 사이의 상호연관성을 규명하고 그 중요성을 밝히는 전략을 살펴보고자 한다. 허혈전처치의 심장보호기전으로서 심장 세포막 K_(ATP)-통로의 역할을 명확히 규명하기 위한 적절한 실험전략의 수립을 통해, 허혈성 심장질환과 관련된 기초의학적 그리고 임상의학적 연구분야에 중요한 실험적 자료가 될 것으로 사료된다.

Nitric Oxide-cGMP-Protein Kinase G Pathway Contributes to Cardioprotective Effects of ATP-Sensitive K<SUP>⁢</SUP> Channels in Rat Hearts

Dang Van Cuong,Nari Kim,Hee Cheol Cho,Euiyong Kim,Jin Han 대한생리학회-대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.2

Ischemic preconditioning (IPC) has been accepted as a heart protection phenomenon against ischemia and reperfusion (I/R) injury. The activation of ATP-sensitive potassium (K<SUB>ATP</SUB>) channels and the release of myocardial nitric oxide (NO) induced by IPC were demonstrated as the triggers or mediators of IPC. A common action mechanism of NO is a direct or indirect increase in tissue cGMP content. Furthermore, cGMP has also been shown to contribute cardiac protective effect to reduce heart I/R-induced infarction. The present investigation tested the hypothesis that K<SUB>ATP</SUB> channels attenuate DNA strand breaks and oxidative damage in an in vitro model of I/R utilizing rat ventricular myocytes. We estimated DNA strand breaks and oxidative damage by mean of single cell gel electrophoresis with endonuclease III cutting sites (comet assay). In the I/R model, the level of DNA damage increased massively. Preconditioning with a single 5-min anoxia, diazoxide (100μM), SNAP (300μM) and 8-(4-Chlorophenylthio)-guanosine-3 ,5 -cyclic monophosphate (8-pCPT-cGMP) (100μM) followed by 15 min reoxygenation reduced DNA damage level against subsequent 30 min anoxia and 60 min reoxygenation. These protective effects were blocked by the concomitant presence of glibenclamide (50μM), 5-hydroxydecanoate (5-HD) (100μM) and 8-(4-Chlorophenylthio)-guanosine-3 ,5 -cyclic monophosphate, Rp-isomer (Rp-8-pCPT-cGMP) (100μM). These results suggest that NO-cGMP-protein kinase G (PKG) pathway contributes to cardioprotective effect of K<SUB>ATP</SUB> channels in rat ventricular myocytes.