Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

A novel illumination system design for application in the integrated screen 3D display

Lin, Chu-Hsun,Lin, Chun-Chuan,Lo, Hsin-Hsiang,Chung, Shuang-Chao,Chen, Tian-Yuan,Wang, Chy-Lin The Korean Infomation Display Society 2010 Journal of information display Vol.11 No.1

A mini-projector prototype employing a LED light source, a nontelecentric structure, and an LCOS panel for application in the integrated 3D display was fabricated. A seamless image was obtained by tilting an array of mini-projectors. Seamless quality was created by the excellent uniformity of the projection intensity on the mini-projector's screen, which was simulated as 98.34%. Great uniformity can be realized by optimizing the design of the light source and the optics configuration, which is the key to such realization.

Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic Activity Contributes to Interferon-Gamma Induced Apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells

Chung, Ting Wen,Tan, Kok-Tong,Chan, Hong-Lin,Lai, Ming-Derg,Yen, Meng-Chi,Li, Yi-Ron,Lin, Sheng Hao,Lin, Chi-Chen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Interferon-gamma (IFN-${\gamma}$) has been used to treat various malignant tumors. However, the molecular mechanisms underlying the direct anti-proliferative activity of IFN-${\gamma}$ are poorly understood. In the present study, we examined the in vitro antitumor activity of IFN-${\gamma}$ on two human non-small-cell lung carcinoma (NSCLC) cell lines, H322M and H226. Our findings indicated that IFN-${\gamma}$ treatment caused a time-dependent reduction in cell viability and induced apoptosis through a FADD-mediated caspase-8/tBid/mitochondria-dependent pathway in both cell lines. Notably, we also postulated that IFN-${\gamma}$ increased indoleamine 2,3-dioxygenase (IDO) expression and enzymatic activity in H322M and H226 cells. In addition, inhibition of IDO activity by the IDO inhibitor 1-MT or tryptophan significantly reduced IFN-${\gamma}$-induced apoptosis and death receptor 5 (DR5) expression, which suggests that IDO enzymatic activity plays an important role in the anti-NSCLC cancer effect of IFN-${\gamma}$. These results provide new mechanistic insights into interferon-${\gamma}$ antitumor activity and further support IFN-${\gamma}$ as a potential therapeutic adjuvant for the treatment of NCSLC.

On the direct insulator-quantum Hall transition in two-dimensional electron systems in the vicinity of nanoscaled scatterers

Liang, Chi-Te,Lin, Li-Hung,Kuang Yoa, Chen,Lo, Shun-Tsung,Wang, Yi-Ting,Lou, Dong-Sheng,Kim, Gil-Ho,Yuan-Huei, Chang,Ochiai, Yuichi,Aoki, Nobuyuki,Chen, Jeng-Chung,Lin, Yiping,Chun-Feng, Huang,Lin, Sh Springer 2011 Nanoscale research letters Vol.6 No.1

<P>A direct insulator-quantum Hall (I-QH) transition corresponds to a crossover/transition from the insulating regime to a high Landau level filling factor ν > 2 QH state. Such a transition has been attracting a great deal of both experimental and theoretical interests. In this study, we present three different two-dimensional electron systems (2DESs) which are in the vicinity of nanoscaled scatterers. All these three devices exhibit a direct I-QH transition, and the transport properties under different nanaoscaled scatterers are discussed.</P>

A novel illumination system design for application in the integrated screen 3D display

Chu-Hsun Lin,Chun-Chuan Lin,Hsin-Hsiang Lo,Shuang-Chao Chung,Tian-Yuan Chen,Chy-Lin Wang 한국정보디스플레이학회 2010 Journal of information display Vol.11 No.1

A mini-projector prototype employing a LED light source, a nontelecentric structure, and an LCOS panel for application in the integrated 3D display was fabricated. A seamless image was obtained by tilting an array of mini-projectors. Seamless quality was created by the excellent uniformity of the projection intensity on the mini-projector’s screen, which was simulated as 98.34%. Great uniformity can be realized by optimizing the design of the light source and the optics configuration, which is the key to such realization.

Description of a new species of the genus Anochetus Mayr (Hymenoptera: Formicidae) from Orchid Island, Taiwan

Chi-Man Leong,Wei-Huang Tsai,Mamoru Terayama,Shiuh-Feng Shiao,Chung-Chi Lin 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

A trap-jaw ant Anochetus lanyuensis sp. nov., belonging to the graeffei species group Brown, 1978, is described onthe basis of worker and gyne castes from Orchid Island (Lanyu), Taiwan. A. lanyuensis sp. nov. is easily distinguishablefrom the other species in the graeffei species group by the large size of its eye, longitudinal striae onthe pronotal disc, and concave dorsal margin of the petiolar node in the frontal view.

In vitro Transport of Fexofenadine·HCl in Deformable Liposomes Across the Human Nasal Epithelial Cell Monolayers

Lin, Hongxia,Lee, Chi-Ho,Shim, Chang-Koo,Chung, Suk-Jae,Kim, Dae-Duk 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

Fexofenadine·HC1 is non-sedating histamine HI receptor antagonist that can he used for the treatment of seasonal allergic rhinitis. The objective of this study was to investigate whether the carriers of defonnable liposomes can enhance the transepithelial permeability of ferofenadine·HCl across the in vitro ALl human nasal monolay et model. Characterization of this model was achieved by bioelectric measurements and morphological studies. The passage 2 and 3 of cell monolayers exhibited the TEER value of 28521482 ohm x cm: on 11 days of seeding and maintained high TEER value for 3 days. The defonnable liposome of fexofenadine.HC1 was prepared with phosphatidylcholine (PC) and cholic acid using extruder method. The mean particle size was about 200 mn and the maximum entrapment efficiency of 33.0% was obtained in the formulation of 1% PC and 100 μg/ml ferofenadine·HC1. The toxicity of the defonnable liposome to human nasal monolayers was evaluated by MTT assay and TEER value change. MTT assay showed that it has no toxic effect on the nasal epithelial cells in 2-hour incubation when the PC concentration was below 1%. However. defonnable liposome could not enhance the transepithelial permeability (Pap,) and cellular uptake of ferofenadine·HC1. In conclusion. the in vitro model could be used in nasal drug transport studies and evaluation of transepithelial permeability of formulations.

Neuroprotective Activity of Vitis thunbergii var. taiwaniana Extracts In Vitro and In Vivo

Chung-Kwe Wang,Lih-Geeng Chen,Chi-Luan Wen,Wen-Chi Hou,Ling-Fang Hung,Shish-Jung Yen,Yi-Jyun Shen,Shyr-Yi Lin,Yu-Chih Liang 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1

Vitis thunbergii var. taiwaniana (VTT) is a wild grape native to Taiwan, where it has been used as a folk medicine. In this study, we found that the branch and leaf ethanol extracts of VTT significantly inhibited the inducible nitric oxide (NO) synthase protein expression and NO production in BV2 microglia. Using primary neuronal cells, kainic acid (KA) significantly increased hydrogen peroxide production in a dose-dependent manner. All four ethanol extracts of VTT significantly decreased hydrogen peroxide production. However, only root and branch ethanol extracts were able to prevent the neuronal cell death induced by KA in vitro. In the animal study, administration of all four plant part extracts of VTT delayed the onset of seizure and decreased the hippocampus neuronal cell loss, and the neuroprotective activity could be ranked as follows: branchleaf>root>trunk. The results suggest that VTT extracts have a potential to prevent neurodegeneration through the antioxidative activity by their ability to inhibit NO and hydrogen peroxide production.

Viability and Functions of Alginate-microencapsulated Islets Isolated from Neonatal Pigs

Lin, Yi-Juain,Wang, Jui-Ping,Chung, Yu-Tung,Sun, Yu-Ling,Chou, Yu-Chi Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

Patients with Type I diabetes mellitus have been treated with porcine insulin for several decades and pigs have recently been deemed an ideal source of microencapsulated islet cells for clinical xenotransplantation. In this study, neonatal pigs were anesthetized and sacrificed prior to a pancreatectomy. Islet cells were isolated from pancreas via collagenase digestion. Islet cells were separated and collected by hand under microscopic guidance. These cells were suspended in 1.4% sodium alginate solution and encapsulated by dropping them into 1.1% calcium chloride solution and in which the round gel in size was 250-400 ${\mu}m$ in diameter. Viability of the microencapsulated islet cells cultured in medium at $37^{\circ}C$ was assessed by MTT assay. Furthermore, insulin released in response to glucose challenge was investigated using an enzyme-linked immunosorbent assay. Secretion of insulin was low in response to the basal glucose solution (4.4 mM) in medium and was significantly higher in response to the high glucose solution (16.7 mM). The viability of microencapsulated islet cells did not differ significantly over a period of 7 days; that is, the increasing pattern of insulin concentration in the culture medium after glucose stimulation interval day was similar throughout the 7 days cultivation. In summary, experimental evidences indicated that the effects of alginate-microencapsulation prolonged survival of the neonatal porcine islets in vitro cultures and the insulin response to glucose of the islets was maintained.

Chemical Constituents from Lobelia chinensis and Their Anti-virus and Anti-inflammatory Bioactivities

Ping-Chung Kuo,Yann-Lii Leu,Tsong-Long Hwang,Ying-Ting Lin,Yuh-Chi Kuo 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.5

In total, forty six compounds, including the novel compound lobechine (1), were characterized from the methanol extracts of Lobelia chinensis. The chemical structures of known metabolites were identified by comparing their spectroscopic and physical data with compounds reported in the literature. The structure of lobechine (1) was comprehensively established with the aid of 1D and 2D NMR spectroscopic analyses. In addition, selected isolates were screened for their inhibition of HSV-1 replication, superoxide anion generation, and elastase release. Among the tested compounds, scoparone (10) exhibited significant inhibition of superoxide anion generation with IC_50 of 6.14 ± 1.97 μM and lobechine (1) exhibited moderate inhibition of elastase release with IC_50 of 25.01 ± 6.95 μM, respectively.