Genome-wide identification and evolutionary analysis of WOX gene family in cucurbit crops

Li Chun,He Zhen,Liang Genyun,Yang Nan,Cai Peng,Liang Ying,Li Yuejian,Fang Chao,Liu Duchen,Xia Feng,Liu Xiaojun 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

The WUSCHEL-related homeobox (WOX) gene family is essential for plant growth and development. However, the identifi cation and evolutionary analysis of WOX genes in the Cucurbitaceae have not been well investigated. In the present work, we performed a genome-wide analysis to identify WOX genes in eleven cucurbit crops. In total, 127 WOX genes were identifi ed in eleven cucurbit crops, and classifi ed into three diff erent clades: the ancient clade, the intermediate clade and the WUS clade. The same clade has comparable gene organization and conserved motif patterns. The cis -acting ele ment analysis revealed that WOX genes were involved in light, hormone and stress in cucurbit crops. Gene duplication analysis indicated that dispersed duplication and whole genome duplication were the two primary causes of WOX genes expansion. There were 24 and 129 gene pairs found within cucurbit crops, and between Cucumis sativus and other cucur bit crops, respectively. After gene duplication, all WOX genes were subjected to substantial purifying selection pressure according to the selective pressure analysis. Functional divergence analysis revealed that ancient and intermediate clades may have descended from some essential amino acid sites rather than diff erent evolution rate. Our research provides a comprehensive analysis of WOX genes which will be helpful for further research into their function study in Cucurbita ceae plants.

Ginsenoside Rg3 in combination with artesunate overcomes sorafenib resistance in hepatoma cell and mouse models

Ying-Jie Chen,Jia-Ying Wu,Yu-Yi Deng,Ying Wu,Xiao-Qi Wang,Amy Sze-man Li,Lut Yi Wong,Xiuqiong Fu,Zhi-Ling Yu,Chun Liang 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

Sorafenib is effective in treating hepatoma, but most patients develop resistance to it. STAT3signaling has been implicated in sorafenib resistance. Artesunate (ART) and 20(R)-ginsenoside Rg3 (Rg3)have anti-hepatoma effects and can inhibit STAT3 signaling in cancer cells. This study aimed to evaluatethe effects of Rg3 in combination with ART (Rg3-plus-ART) in overcoming sorafenib resistance, and toexamine the involvement of STAT3 signaling in these effects. Methods: Sorafenib-resistant HepG2 cells (HepG2-SR) were used to evaluate the in vitro anti-hepatomaeffects of Rg3-plus-ART. A HepG2-SR hepatoma-bearing BALB/c-nu/nu mouse model was used to assessthe in vivo anti-hepatoma effects of Rg3-plus-ART. CCK-8 assays and Annexin V-FITC/PI double stainingwere used to examine cell proliferation and apoptosis, respectively. Immunoblotting was employed toexamine protein levels. ROS generation was examined by measuring DCF-DA fluorescence. Results: Rg3-plus-ART synergistically reduced viability of, and evoked apoptosis in HepG2-SR cells, andsuppressed HepG2-SR tumor growth in mice. Mechanistic studies revealed that Rg3-plus-ART inhibitedactivation/phosphorylation of Src and STAT3 in HepG2-SR cultures and tumors. The combination alsodecreased the STAT3 nuclear level and induced ROS production in HepG2-SR cultures. Furthermore, overactivation of STAT3 or removal of ROS diminished the anti-proliferative effects of Rg3-plus-ART, andremoval of ROS diminished Rg3-plus-ART's inhibitory effects on STAT3 activation in HepG2-SR cells. Conclusions: Rg3-plus-ART overcomes sorafenib resistance in experimental models, and inhibition of Src/STAT3 signaling and modulation of ROS/STAT3 signaling contribute to the underlying mechanisms. Thisstudy provides a pharmacological basis for developing Rg3-plus-ART into a novel modality for treatingsorafenib-resistant hepatoma.

Physicochemical Properties of Low-Phytate Rice Cultivar, Sang-gol

Li, Chun-Ying,Li, He,Choi, Jae-Keun,Kim, Hee-Woong,Hong, Soon-Kwan,Xu, Zhihao,Rhee, Hae-Ik The Korean Society for Applied Biological Chemisty 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.1

Phytic acid in grains affects the bioavailability of minerals and nutrients in monogastric animals. Physicochemical properties of Sang-gol, a low-phytate rice cultivar developed to decrease anti-nutrient effect of phytic acid, were compared with those of its parent rice cultivar Il-pum. The amylose content of Sang-gol was lower, but its crude protein content was not significantly different compare with Il-pum. Texture profiles of cooked rice, except for hardness, adhesiveness and chewiness, did not show any differences. The hardness, adhesiveness and chewiness of the Sang-gol were lower than that of Il-pum. Pasting characteristics, peak viscosity, trough, and final viscosity of Sang-gol were higher, and breakdown was lower, as compared to Il-pum. Setback of Il-pum was significantly (p <0.01) lower than Il-pum. The pasting temperatures of Sang-gol and Il-pum were very similar (68.1 and $68.0^{\circ}C$), respectively.

Physicochemical Properties of Low-Phytate Rice Cultivar, Sang-gol

Chun-Ying Li,He Li,Jae-Keun Choi,Hee-Woong Kim,홍순관,Zhihao Xu,이해익 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.1

Phytic acid in grains affects the bioavailability of minerals and nutrients in monogastric animals. Physicochemical properties of Sang-gol, a low-phytate rice cultivar developed to decrease anti-nutrient effect of phytic acid, were compared with those of its parent rice cultivar Il-pum. The amylose content of Sang-gol was lower, but its crude protein content was not significantly different compare with Il-pum. Texture profiles of cooked rice, except for hardness, adhesiveness and chewiness, did not show any differences. The hardness, adhesiveness and chewiness of the Sang-gol were lower than that of Il-pum. Pasting characteristics, peak viscosity, trough, and final viscosity of Sanggol were higher, and breakdown was lower, as compared to Ilpum. Setback of Il-pum was significantly (p <0.01) lower than Ilpum. The pasting temperatures of Sang-gol and Il-pum were very similar (68.1 and 68.0oC), respectively.

Astragalus polysaccharide: a review of its immunomodulatory effect

Chun-xiao Li,Ying Liu,Yu-zhen Zhang,Jing-chun Li,Jiang Lai 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.6

The Astragalus polysaccharide is an importantbioactive component derived from the dry root of Astragalusmembranaceus . This review aims to provide a comprehensiveoverview of the research progress on the immunomodulatoryeff ect of Astragalus polysaccharide and providevaluable reference information. We review the immunomodulatoryeff ect of Astragalus polysaccharide on central andperipheral immune organs, including bone marrow, thymus,lymph nodes, spleen, and mucosal tissues. Furthermore, theimmunomodulatory eff ect of Astragalus polysaccharide on avariety of immune cells is summarized. Studies have shownthat Astragalus polysaccharide can promote the activities ofmacrophages, natural killer cells, dendritic cells, T lymphocytes,B lymphocytes and microglia and induce the expressionof a variety of cytokines and chemokines. The immunomodulatoryeff ect of Astragalus polysaccharide makesit promising for the treatment of many diseases, includingcancer, infection, type 1 diabetes, asthma, and autoimmunedisease. Among them, the anticancer effect is the mostprominent. In short, Astragalus polysaccharide is a valuableimmunomodulatory medicine, but further high-qualitystudies are warranted to corroborate its clinical effi cacy.

Compound HRAS/PIK3CA Mutations in Chinese Patients with Alveolar Rhabdomyosarcomas

Liu, Chun-Xia,Li, Xiao-Ying,Li, Cheng-Fang,Chen, Yun-Zhao,Cui, Xiao-Bin,Hu, Jian-Ming,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

The rhabdomyosarcoma (RMS) is the most common type of soft tissue tumor in children and adolescents; yet only a few screens for oncogenic mutations have been conducted for RMS. To identify novel mutations and potential therapeutic targets, we conducted a high-throughput Sequenom mass spectrometry-based analysis of 238 known mutations in 19 oncogenes in 17 primary formalin-fixed paraffin-embedded RMS tissue samples and two RMS cell lines. Mutations were detected in 31.6% (6 of 19) of the RMS specimens. Specifically, mutations in the NRAS gene were found in 27.3% (3 of 11) of embryonal RMS cases, while mutations in NRAS, HRAS, and PIK3CA genes were identified in 37.5% (3 of 8) of alveolar RMS (ARMS) cases; moreover, PIK3CA mutations were found in 25% (2 of 8) of ARMS specimens. The results demonstrate that tumor profiling in archival tissue samples is a useful tool for identifying diagnostic markers and potential therapeutic targets and suggests that these HRAS/ PIK3CA mutations play a critical role in the genesis of RMS.

논문 : 황기 추출물의 항산화 효과

이춘영 ( Chun Ying Li ),이해익 ( Hae Ik Rhee ) 강원대학교 농업과학연구소 2004 강원 농업생명환경연구 Vol.15 No.-

張九成與朱熹愼獨思想比較

이춘영 ( Li Chun-ying ) 충남대학교 유학연구소 2018 儒學硏究 Vol.44 No.-

愼獨作爲儒家重要的修養工夫,宋代理學和心學對其各有闡發。朱熹將《中庸》中的愼獨和戒懼解讀爲兩種工夫,愼獨是已發之后爲善去惡的工夫,戒懼是心存敬畏、主敬存養的工夫;張九成認爲愼獨與戒懼相同,都是對天理本心的體認,這是一種直取本體的工夫路徑。二人愼獨思想存在差異的根本原因,是理學和心學對本體理解不同。朱熹之“性卽理”,是寂然不動的形而上本體;張九成之“心卽理”,是貫穿形而上形而下,貫穿已發未發的本體。因而朱熹的工夫論中必鬚要有心的思維作用,爲學與主敬幷重,方能貫通;張九成的工夫論中,心卽本體卽工夫,以心識心。朱熹與張九成愼獨思想的差異,正是心學與理學工夫論的根本分岐。 Restraining in privacy is an important cultivation effort of Confucianism, which was further developed by neo-Confucianism. When Zhu Xiinterpreted The Doctrine of the Mean, he distinguished Restraining in privacy from Being cautious and apprehensive (戒愼恐懼). Restraining in privacy was an effort when thoughts /emotions have arisen, but being cautious and apprehensive was an effort when thoughts /emotions haven’t arisen. Different from Zhu Xi, Zhang Jiucheng took Restraining in privacy and Being cautious and apprehensive as one effort, which was way to reach noumenon. The difference between their theories of effort rooted in the difference of their theory of noumenon. Zhu Xi regarded human nature as heaven principle, and Zhang Jiucheng regarded heart-mind as heaven principle. In Zhu Xi’s thought, as noumenon, human nature was only in the state when thoughts /emotions haven’t arisen. But in Zhang Jiucheng’s thought, heart-mind was running through all the time, which could show itself no matter when thoughts /emotions have arisen or not. The noumenon is also the effort. The different between Zhu Xi and Zhang Jiucheng’s thought, is exactly the fundamental disagreement between the School of Principle and the School of Mind.

연구논문 : 중국의 특수미 연구 현황 및 전망

이춘영 ( Chun Ying Li ),최용환 ( Yong Hwan Choi ),이상복 ( Sang Bok Lee ),양창인 ( Chang Ihn Yang ),이점호 ( Jeom Ho Lee ),김홍열 ( Hong Yeol Kim ),김연규 ( Yeon Gyu Kim ) 한국국제농업개발학회 2008 韓國國際農業開發學會誌 Vol.20 No.4

Clinical Analysis of Stages of HBV Infection in 100 Cases of Lymphoma

Tang, Yang,Sun, Li-Guang,Liu, Chun-Shui,Li, Yu-Ying,Jin, Chun-Hui,Li, Dan,Bai, Ou Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Objective: HBV infection may cause damage to the immune system and induce lymphomas as a result. Some scholars have indicated that HBsAg(+) reflecting HBV infection may have a relationship with lymphoma development. This study was designed to find out the specific stage of HBV infection which may be related to lymphoma. Methods: HBV serum markers, including HBsAg, HBsAb, HBeAg, HBeAb, HBcAb were tested among 100 lymphoma patients and 100 other patients who were diagnosed with non-lymphoma diseases in the First Hospital of Jilin University from 2010.1.1 to 2012.12.31. Three subgroups were established depending on different combinations of HBV serum markers. Subgroup 1 was HBsAg(+) representing the early stage of HBV infection. Subgroup 2 was HbsAb(+) representing convalescence and Subgroup 3 was "HbsAg and HbsAb negative combined with other positive markers" representing the intermediate stage of HBV infection. Chi square tests were used to compare the rates of three subgroups in lymphoma and control groups. Results: The rates of Subgroup were 13% and 5% respectively, an association between HBsAg and lymphoma being found (P<0.05). There was no difference between rate of Subgroup 2 of lymphoma group (15%) and that of control group (16%). In lymphoma group and control group, the rate of Subgroup 3 was different (12% vs 4%). This evidence was not specific to T cell lymphoma, B cell lymphoma or Hodgkin's lymphoma. Conclusions: Among serum markers of HBV, the combination of serum markers representing the early stage and intermediate stage of HBV infection have a relationship with lymphoma. Convalescence from HBV infection appears to have no relationship with lymphoma.