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조석환경 변화에 의한 표층퇴적물의 시.공간적 분포 변화: 한국 서해안의 무안만
류상옥,장진호,이희준,조영조,최옥인,Ryu, Sang-Ock,Chang, Jin-Ho,Lee, Hee-Jun,Jo, Yeong-Jo,Choi, Ok-In 한국해양학회 2009 바다 Vol.14 No.1
하구언과 방조제 건설에 따라 고극조위 상승과 같은 조석환경 변화가 심한 무안만에서 표층퇴적물의 분포 변화와 변화 원인을 규명하기 위하여 퇴적물 시료를 채취하고 조류관측을 실시하였다. 무안만의 표층퇴적물은 1998년에 비해 역과 사의 조립질퇴적물의 함량이 증가하고 실트의 함량이 감소하여 퇴적물의 평균입도는 6.2${\phi}$에서 5.8${\phi}$로 조립해지는 경향을 보였다. 조립질퇴적물의 함량 증가와 실트의 함량 감소는 만조선 주변을 중심으로 광범위하게 나타났으며, 특히 동암리와 구로리, 압해도 조간대에서 두드러지게 나타났다. 만조선 주변의 조립화 현상은 일차적으로 하구언 및 방조제 건설에 따른 고극조위 상승에 기인하는 것으로 해석된다. 한편, 서측 만입구 주변에서 세립질퇴적물의 함량이 증가하는 현상은 인공구조물(교각) 건설에 따른 조류속의 감소와 위상차에 의한 조류의 방향 변화 등 수류의 변형에 기인하는 것으로 판단된다. Muan Bay has extensively undertaken artificial influences through a series of construction of dams and dykes nearby resulting in a substantial increase in highest high water level. To unravel sedimentary responses to the water level rise, we collected a number of surface sediment samples and hydrodynamic data from in-situ observations of tidal currents in Muan Bay in 1998 and 2007. Comparison of the data between 1998 and 2007 shows that the relative content of coarse fractions, sand and gravel, in the sediment samples has increased with a decreased mean size, on average, from 6.2 to 5.8${\phi}$ at the expense of silt contents. Such a coarsening trend, which is more evident around high water level, particularly on the tidal flats of Dongam-ri, Guro-ri and Aphae-do, is most likely attributable to rising water level. On the other hand, an increase of fine fractions locally in the western entrance of the bay can be explained with changes in characteristics of tidal currents imposed by construction of bridges therein, such as decreased velocities and varied direction and phase of the tidal currents.
분화된 HL60 세포에서 Granulocyte - Macrophage Colony - Stimulating Factor 에 의한 95kDa 단백질의 Tyrosine 잔기 인산화
이영한,김정옥,민도식,김희숙,김용식,이창연,류성호,서판길 ( Young Han Lee,Jeong Ock Kim,Do Sik Min,Hee Sook Kim,Yong Sik Kim,Chang Youn Lee,Sung Ho Ryu,Pann Ghill Suh ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multipotent cytokine which stimulates the proliferation and differentiation of various lineage of hematopoietic cells. We examined whether GM-CSF stimulates protein phosphorylation in HL60 cells pretreated with differentiation-inducing factors such as PMA, 1,25-(OH)₂VD₃ and DMSO. GM-CSF induced tyrosine phosphorylation of 95 kDa protein in PMA- or 1,25-(OH)₂VD₃ but not in DMSO-pretreated cells. Tyrosine phosphorylation of 95 kDa was detected at 1 min and 2 min after stimulation of GM-CSF in PMA- and 1,25-(OH)₂VD₃-pretreated cells, respectively. Kinase activity which phosphorylates tyrosine residues) of the 95 kDa protein appeared to increase in a time dependent manner in PMA-pretreated cells, whereas the expression level of 95 kDa protein was not changed. We also observed that 95 kDa protein was autophosphorylated in immunecomplex kinase assay, suggesting that this 95 kDa protein may be tyrosine kinase which is activated in lineage specific manner. These results suggest that 95 kDa protein may be involved in an early signal transduction pathway of GM-CSF.
분화된 HL60 세포에서 Granulocyte-Macrophage Colony-Stimulating Factor에 의한 95 kDa 단백질의 Tyrosine잔기 인산화
이영한,김정옥,민도식,김희숙,김용식,이창연,류성호,서판길,Lee, Young-Han,Kim, Jeong-Ock,Min, Do-Sik,Kim, Hee-Sook,Kim, Yong-Sik,Lee, Chang-Youn,Ryu, Sung-Ho,Suh, Pann-Ghill 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5
사람의 granulocyte-macrophage colony-stimulating factor(GM-CSF)는 세포 표면에 존재하는 특이 수용체 자극을 통하여 각종 분화단계에 있는 골수세포의 성장과 분화에 중요한 cytokine이다. HL60 세포에 분화 유도 인자인 phorbol 12-myristate 13-acetate(PMA), $1{\alpha}$,25-dihydroxyvitamine $D_3$[1,25-$(OH)_{2}VD_{3}$] 및 dimethyl sulfoxide(DMSO) 등을 전처리한 후 GM-CSF 자극에 대해 특정 분화단계에서만 활성화되는 세포내 단백질을 조사하였다. SH2-SH3 domain을 인지하는 F7-2 항체로 면역 침전하여 면역 블롯한 결과 미분화 세포와 DMSO를 처리한 세포에서는 GM-CSF 자극에 의해 tyrosine 인산화되는 단백질을 발견할 수 없었지만, PMA와 1,25-$(OH)_2VD_3$를 전처리한 세포에서는 95kDa 단백질의 tyrosine 인산화가 일어남을 관찰하였다. Kinetics 분석결과 95 kDa 단백질의 tyrosine 인산화 반응은 GM-CSF 처리 후 1분 이내에, 1,25-$(OH)_{2}VD_{3}$를 전처리한 세포에서는 2분 이내에 나타나는 신속한 반응이었다. 이 단백질이 세포에서 발현되는 양은 PMA 전처리 시간에 무관하게 일정하였으나 GM-CSF에 의한 인산화는 PMA 전처리 시간에 따라 변화하였다. 또한, 95 kDa 단백질은 in vitro에서도 autophosphorylation되었다. 이러한 결과로부터 95 kDa 단백질은 PMA나 1,25-$(OH)_{2}VD_{3}$로 분화 유도된 HL60 세포에서 GM-CSF의 초기 신호전달 경로에 관여하고 있음을 추측할 수 있었다. Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multipotent cytokine which stimulates the proliferation and differentiation of various lineage of hematopoietic cells. We examined whether GM-CSF stimulates protein phosphorylation in HL60 cells pretreated with differentiation-inducing factors such as PMA, 1,25-$(OH)_{2}VD_{3}$ and DMSO. GM-CSF induced tyrosine phosphorylation of 95 kDa protein in PMA- or 1,25-$(OH)_{2}VD_{3}$ but not in DMSO-pretreated cells. Tyrosine phosphorylation of 95 kDa was detected at 1 min and 2 min after stimulation of GM-CSF in PMA- and 1,25-$(OH)_{2}VD_{3}$-pretreated cells, respectively. Kinase activity which phosphorylates tyrosine residue(s) of the 95 kDa protein appeared to increase in a time dependent manner in PMA-pretreated cells, whereas the expression level of 95 kDa protein was not changed. We also observed that 95 kDa protein was autophosphorylated in immunecomplex kinase assay, suggesting that this 95 kDa protein may be tyrosine kinase which is activated in lineage specific manner. These results suggest that 95 kDa protein may be involved in an early signal transduction pathway of GM-CSF.
Effect of Endothelin-1 on Proliferation and Differentiation of Rat Tracheal Epithelial Cells
Chang Soo Kim,Sae Ock Oh,Jae Suk Woo,Jin Sup Jung,Yong Keun Kim,Sang Ho Lee 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.6
<P> A number of substances involved in the proliferation and differentiation of the tracheobronchial epithelium have been identified. The defects in the control of the proliferation and differentiation of tracheobronchial epithelial cells appear to constitute crucial steps in the transition of normal cells to neoplastic ones. Endothelin-1 is produced by tracheal epithelial cells, and its receptors are present in tracheal epithelial cells. However, the effect of endothelin-1 on the proliferation and differentiation of tracheal epithelial cells has not been clearly elucidated. This study was undertaken to investigate these actions of endothelin-1 in primary cultured cells of rat tracheal epithelia. Endothelin-1 stimulated proliferation of tracheal epithelial cells 1.5-fold when compared with that of control cells. Endothelin-1 increased mitogen-activated protein kinase (MAPK) activity. Herbimycin A, a tyrosine kinase inhibitor, inhibited endothelin-1-induced proliferation of epithelial cells. The treatment of endothelin-1 during the primary culture of tracheal epithelial cells increased AB-PAS-stained cell population and ciliated cell population 6.5 fold and 1.5 fold, respectively, when compared with those in control cells. The responsiveness to carbachol and forskolin in the Cl<SUP>-</SUP> secretion was increased 1.7 and 1.9 fold, respectively, in the endothelin-treated epithelial cells. These results indicated that endothelin-1 increases proliferation via MAPK pathway and stimulates differentiation to secretory and ciliated cells in rat tracheal epithelial cells.
A Study on Current Status of Pesticide Residues in Commercial Agricultural Products, 2001
Ock-Kyung Chun,Kee-Young Shin,Jib-Ho Lee,Ju-Sung Bak,Tae-Hee Cho,Tae-Rang Kim,Ouk-Hee Kim,Min-Su Chang,In-Suck Hong,Yeo-Jun Son,Sung-Ae Cho,Young-Hee Choi,Young-Ho Seo,Bok-Soon Kim,Hee-Gon Kang 한국식품과학회 2002 Food Science and Biotechnology Vol.11 No.6
SUNG HA PARK,CHANG-OCK LEE,JOOYOUNG HAHN 한국산업응용수학회 2014 Journal of the Korean Society for Industrial and A Vol.18 No.2
We propose a variational segmentation model based on statistical information of intensities in an image. The model consists of both a local region-based energy and a global region-based energy in order to handle misclassification which happens in a typical statistical variational model with an assumption that an image is a mixture of two Gaussian distributions. We find local ambiguous regions where misclassification might happen due to a small difference between two Gaussian distributions. Based on statistical information restricted to the local ambiguous regions, we design a local region-based energy in order to reduce the misclassification. We suggest an algorithm to avoid the difficulty of the Euler-Lagrange equations of the proposed variational model.
Sung In Kyoung,Oh Chang Jin,Lee Eung Suk,Kim Ock Hyun The Korean Society of Mechanical Engineers 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.8
Generally, the optical components are fabricated by grinding, lapping, and polishing. And, those processes take long time to obtain such a high surface quality. Therefore, in the case of large optical component, the on-machine inspection (OMI) is essential. Because, the work piece is fragile and difficult to set up for fabricating and measuring. This paper is concerned about a swing-arm method for measuring surface profile of large optical concave mirror. The measuring accuracy and uncertainty for suggested method are studied. The experimental results show that this method is useful specially in lapping process with the accuracy of $3\~5\;{\mu}m$. Those inspection data are provided for correcting the residual figuring error in lapping or polishing processes.