BF-30 effectively inhibits ciprofloxacin-resistant bacteria in vitro and in a rat model of vaginosis

Wang, Jing,Li, Bing,Li, Yang,Dou, Jie,Hao, Qingru,Tian, Yuwei,Wang, Hui,Zhou, Changlin 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7

Bacterial infections are becoming increasingly difficult to treat due to the increasing number of multidrug-resistant strains. Cathelicidin-BF (BF-30) is a cathelicidin-like antimicrobial peptide and exhibits broad antimicrobial activity against bacteria. In the present study, the antibacterial activity of BF-30 against ciprofloxacin-resistant Escherichia coli and Staphylococcus aureus was examined, and the protective effects of this peptide against these bacteria in rats with bacterial vaginosis were identified for the first time. The data showed that BF-30 had effective antimicrobial activities against ciprofloxacin-resistant E. coli and S. aureus. The minimal inhibitory concentrations for both bacterial strains were $16{\mu}g/ml$, and the minimal bactericidal concentrations were 64 and $128{\mu}g/ml$, respectively. A time course experiment showed that the CFU counts rapidly decreased after BF-30 treatment, and the bacteria were nearly eliminated within 4 h. BF-30 could reduce the fold change (CFU/ml) in local colonization by drug-resistant E. coli and S. aureus to 0.01 at a dose of 0.8 mg/kg/day in the rats' vaginal secretions. In addition, BF-30 induced membrane permeabilization and bound to the genomic DNA, interrupting protein synthesis. Taken together, our data demonstrate that BF-30 has potential therapeutic value for the prevention and treatment of bacterial vaginosis.

Collusion Suspicion Among Bidders of Construction Supervision Projects in China: Based on Bidding Big Data and Complex Networks

Bing Wang,Jiwei Zhu,Jiancang Xie,Biao Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.1

Collusion is unethical and anti-competitive behavior, which has become a prominent issue in the construction industry. However, existing studies were unable to identify the degree of collusion suspected for each bidder, which has little significance to regulators. Therefore, we develop a bidder network for the supervision project in Shaanxi Province, China, and analyze the behavioral characteristics of bidders from three levels. The results indicate that the bidding network is low-density, scale-free, and small-world at the macro level. The possibility of potential collusion is extremely high among supervisory enterprises. At the meso level, the bidder network can be divided into multiple communities and subgraphs with a high degree of cohesion, and the level of conspiracy suspicion was divided into four categories. At the micro level, the number of co-bidding among the bidders with the highest suspicion was extremely high, with higher winning bid times and significantly abnormal regularity of the bidding price, which is typical of bid-winning rotation practice. These results provide an effective method for Chinese government regulators, which are conducive to the formation of a good collusion regulatory system worldwide.

Efficacy and Safety of Sorafenib for Advanced Non-Small Cell Lung Cancer: a Meta-analysis of Randomized Controlled Trials

Wang, Wei-Lan,Tang, Zhi-Hui,Xie, Ting-Ting,Xiao, Bing-Kun,Zhang, Xin-Yu,Guo, Dai-Hong,Wang, Dong-Xiao,Pei, Fei,Si, Hai-Yan,Zhu, Man Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Many clinical trials have been conducted to evaluate sorafenib for the treatment of advanced NSCLC, but the results for efficacy have been inconsistent. The aim of this study was to evaluate the efficacy and safety of sorafenib in patients with advanced NSCLC in more detail by meta-analysis. Methods: This meta-analysis of randomized controlled trials (RCTs) was performed after searching PubMed, EMBASE, ASCO Abstracts, ESMO Abstracts, and the proceedings of major conferences for relevant clinical trials. Two reviewers independently assessed the quality of the trials. Outcomes analysis were disease control rate (DCR), progression- free survival (PFS), overall survival (OS) with 95% confidence intervals (CI) and major toxicity. Subgroup analysis was conducted according to sorafenib monotherapy, in combination with chemotherapy or EGFR-TKI to investigate the preferred therapy strategy. Results: Results reported from 6 RCTs involving 2, 748 patients were included in the analysis. Compared to sorafenib-free group, SBT was not associated with higher DCR (RR 1.31 (0.96- 1.79), p=0.09), PFS (HR 0.82 (0.66-1.02), p=0.07) and OS (HR 1.01 (0.92-1.12), p=0.77). In terms of subgroup results, sorafenib monotherapy was associated with significant superior DCR and longer PFS, but failed to show advantage with regard to OS. Grade 3 or greater sorafenib-related adverse events included fatigue, hypertension, diarrhea, oral mucositis, rash and HFSR. Conclusions: SBT was revealed to yield no improvement in DCR, PFS and OS. However, sorafenib as monotherapy showed some activity in NSCLC. Further evaluation may be considered in subsets of patients who may benefit from this treatment. Sorafenib combined inhibition therapy should be limited unless the choice of platinum-doublet regimen, administration sequence or identification of predictive biomarkers are considered to receive better anti-tumor activity and prevention of resistance mechanisms.

Assessing metabolic properties of dairy cows fed low quality straws by integrative arterial and venous metabolomics

Wang Bing,Yu Zhu,Liu Jian-Xin 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: This study was conducted to reveal potential metabolic differences of dairy cows fed corn stover (CS) and rice straw (RS) instead of alfalfa hay (AH) as main forage source. Methods: Thirty multiparous mid-late lactation Holstein dairy cows were selected and randomly assigned to three diets, AH, CS, or RS (n = 10). After 13 weeks of the feeding trial, coccygeal arterial and superficial epigastric venous plasma samples were collected before morning feeding for gas chromatography time-of-flight/mass spectrometry analyses. Results: In the artery, 8 and 13 metabolites were detected as differential metabolites between AH and CS, and between AH and RS, respectively. The relative abundance of phenylpropanoate (log2fold change [FC]) = 1.30, 1.09), panthenol (log2FC = 2.36, 2.20), threitol (log2FC = 1.00, 1.07), and 3,7,12-trihydroxycoprostane (log2FC = 0.79, 0.78) were greater in both CS and RS than in AH, and tyrosine (log2FC = –0.32), phenylalanine (log2FC = –0.30), and pyruvic acid (log2FC = –0.30) were lower in RS than in AH. In the vein, 1 and 7 metabolites were detected as differential metabolites between AH and CS, and between AH and RS, respectively. By comparing AH and RS, we found that metabolic pathways of phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism were enriched by integrative artery and vein analysis. Furthermore, AH and RS, arterial phenylpropanoate and 4-hydroxyproline were positively, and phenylalanine was negatively correlated with milk urea nitrogen. Finally, in AH and CS, arterial panthenol was negatively correlated with feed efficiency. Conclusion: Arterial metabolic profiles changed more than those in the veins from animals on three forage diets, differing in amino acids. We found that phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism were restricted when cows were fed low-quality cereal straw diets.

An Approach for Reducing Cutting Energy Consumption with Ultra-High Speed Machining of Super Alloy Inconel 718

Bing Wang,Zhan-qiang Liu,Qinghua Song,Yi Wang,iaoping Ren 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.7 No.1

High energy efficient machining is beneficial for sustainable manufacture which is an important target pursued by workshops. The paper aims to prove the feasibility for reducing cutting energy consumption with ultra-high speed machining (UHSM) of super alloy Inconel 718. Firstly, orthogonal cutting experiments of Inconel 718 are performed with a wide cutting speed range. Through analyses of chip micrographs, it is found that plastic deformation accompanied with ductile fracture contributes to serrated chip formation, while fragmented chips are produced through brittle fracture. The results demonstrate that brittle regime machining can be realized for Inconel 718 with ultra-high cutting speeds. Secondly, considering varied deformation behavior for chips with different morphologies, specific cutting energy models for HSM under varied cutting parameters are developed and verified. Then the influences of cutting speed and uncut chip thickness on cutting energy consumption during HSM are revealed. The research results demonstrate the practicability for reducing cutting energy consumption with brittle regime machining of Inconel 718. At last, the relationship between AE signals and cutting energy consumption is explored, and it proves that AE signals are applicable in monitoring cutting energy consumption. The research can provide guidance for energy saving through optimizing cutting parameters.

AN ULTRA-LUMINOUS QUASAR AT <i>z</i> = 5.363 WITH A TEN BILLION SOLAR MASS BLACK HOLE AND A METAL-RICH DLA AT <i>z</i> ∼ 5

Wang, Feige,Wu, Xue-Bing,Fan, Xiaohui,Yang, Jinyi,Cai, Zheng,Yi, Weimin,Zuo, Wenwen,Wang, Ran,McGreer, Ian D.,Ho, Luis C.,Kim, Minjin,Yang, Qian,Bian, Fuyan,Jiang, Linhua IOP Publishing 2015 ASTROPHYSICAL JOURNAL LETTERS - Vol.807 No.1

<P>We report the discovery of an ultra-luminous quasar J030642.51+185315.8 (hereafter J0306+1853) at redshift 5.363, which hosts a supermassive black hole with M-BH = (1.07 +/- 0.27) x 10(10) M-circle dot. With an absolute magnitude M-1450 = -28.92 and a bolometric luminosity L-bol similar to 3.4 x 10(14) L-circle dot, J0306+1853 is one of the most luminous objects in the early universe. It is not likely to be a beamed source based on its small flux variability, low radio loudness, and normal broad emission lines. In addition, a z = 4.986 damped Ly alpha system (DLA) with [M/H] = -1.3 +/- 0.1, among the most metal-rich DLAs at z greater than or similar to 5, is detected in the absorption spectrum of this quasar. This ultra-luminous quasar puts strong constraints on the bright end of the quasar luminosity function and massive end of the black hole mass function. It will provide a unique laboratory for the study of BH growth and the co-evolution between a BH and the host galaxy with multi-wavelength follow-up observations. The future high-resolution spectra will give more insight into the DLA and other absorption systems along the line of sight of J0306+1853.</P>

Wintersweet‐Flower‐Like CoFe ₂ O ₄ /MWCNTs Hybrid Material for High‐Capacity Reversible Lithium Storage

Wang, Ying,Park, Jinsoo,Sun, Bing,Ahn, Hyojun,Wang, Guoxiu Wiley (John WileySons) 2012 Chemistry - An Asian Journal Vol.7 No.8

<P>CoFe(2)O(4)/multiwalled carbon nanotubes (MWCNTs) hybrid materials were synthesized by a hydrothermal method. Field emission scanning electron microscopy and transmission electron microscopy analysis confirmed the morphology of the as-prepared hybrid material resembling wintersweet flower 'buds on branches', in which CoFe(2)O(4) nanoclusters, consisting of nanocrystals with a size of 5-10 nm, are anchored along carbon nanotubes. When applied as an anode material in lithium ion batteries, the CoFe(2)O(4)/MWCNTs hybrid material exhibited a high performance for reversible lithium storage. In particular, the hybrid anode material delivered reversible lithium storage capacities of 809, 765, 539, and 359 mA h g(-1) at current densities of 180, 450, 900, and 1800 mA g(-1), respectively. The superior performance of CoFe(2)O(4)/MWCNTs hybrid materials could be ascribed to the synergistic pinning effect of the wintersweet-flower-like nanoarchitecture. This strategy could also be applied to synthesize other metal oxide/CNTs hybrid materials as high-capacity anode materials for lithium ion batteries.</P>

Research on 3D Reconstruction Method ACL-based

Bing Wang,Chunlan Zhao 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

Anterior cruciate ligament (ACL) 3D reconstruction is a challenging problem for both orthopedic and sports surgeons. In this paper, to investigate the method of quickly establishing 3D digital model of ACL using 2D magnetic resonance images (MRI) and 2D computed tomography (CT) images, we propose an new scene-based segmentation 3D reconstruction method for anterior cruciate ligament. First, CCD camera is anglicized for 3D reconstruction method. Second, scene segmentation method is proposed to get image patch set. Third, the distance is calculated between the image patch and viewpoint of CCD camera. Finally, the proposed method is done for scene reconstruction. The performance of the proposed model is demonstrated on various 2D MRI and CT images. A relatively reliable ACL 3D reconstruction results indicate the validity of our method. The proposed method can provide a complete set of computer aided ACL operation system and realizing the individualized anatomical reconstruction of ACL.

Involvement of Amino Acids Flanking Glu^7.32 of the Gonadotropin-releasing Hormone Receptor in the Selectivity of Antagonists

Wang, Cheng bing,Oh, Da young,Maiti, Kaushik,Kwon, Hyuk bang,Cheon, Jun,Hwang, Jong iIk,Seong, Jae young Korean Society for Molecular Biology 2008 Molecules and cells Vol.25 No.1

The Glu/Asp^7.32 residue in extracellular loop 3 of the mammalian type-I gonadotropin-releasing hormone receptor (GnRHR) interacts with Arg⁸ of GnRH-I, conferring preferential ligand selectivity for GnRH-I over GnRH-II. Previously, we demonstrated that the residues (Ser and Pro) flanking Glu/Asp^7.32 also play a role in the differential agonist selectivity of mammalian and non-mammalian GnRHRs. In this study, we examined the differential antagonist selectivity of wild type and mutant GnRHRs in which the Ser and Pro residues were changed. Cetrorelix, a GnRH-I antagonist, and Trptorelix-2, a GnRH-II antagonist, exhibited high selectivity for mammalian type-I and non-mammalian GnRHRs, respectively. The inhibitory activities of the antagonists were dependent on agonist concentration and subtype. Rat GnRHR in which the Ser-Glu-Pro (SEP) motif was changed to Pro-Glu-Val (PEV) or Pro-Glu-Ser (PES) had increased sensitivity to Trptorelix-2 but decreased sensitivity to Cetrorelix. Mutant bullfrog GnRHR-1 with the SEP motif had the reverse antagonist selectivity, with reduced sensitivity to Trptorelix-2 but increased sensitivity to Cetrorelix. These findings indicate that the residues flanking Glu^7.32 are important for antagonist as well as agonist selectivity.

Residual bearing capacity of steel-concrete composite beams under fatigue loading

Bing Wang,Xiaoling Liu,Ping Zhuge 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.77 No.4

This study was conducted to investigate the residual bearing capacity of steel-concrete composite beams under highcycle fatigue loading through experiments and theoretical analysis. Six test beams with stud connectors were designed and fabricated for static, complete fatigue, and partial fatigue tests. The failure modes and the degradation of several mechanical performance indicators of the composite beams under high-cycle fatigue loading were analyzed. A calculation method for the residual bearing capacity of the composite beams after certain quantities of cyclic loading cycles was established by introducing nonlinear fatigue damage models for concrete, steel beam, and shear connectors beginning with the material residual strength attenuation process. The results show that the failure mode of the composite beams under the given fatigue load appears to be primarily affected by the number of cycles. As the number of fatigue loadings increases, the failure mode transforms from midspan concrete crushing to stud cutting. The bearing capacity of a 3.0-m span composite beam after two million fatigue cycles is degraded by 30.7% due to premature failure of the stud. The calculated values of the residual bearing capacity method of the composite beam established in this paper agree well with the test values, which indicates that the model is feasibly applicable.