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        Short Communications : Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

        ( Md. Siddiqur Rahman ),( Amitavo Chakrabartty ),( Taohidul Islam ),( Roma Rani Sarker ),( Me Alam1,Muhammad Jasim Uddin ),( Laila Akther ),( Hee Jong Song ) 한국가축위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.2

        Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise seroprevalence was found 3.0% in 2∼3 years age group and 2.0% in 4∼8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

      • SCISCIESCOPUS

        Effect of Rhizobium sp. BARIRGm901 inoculation on nodulation, nitrogen fixation and yield of soybean (Glycine max) genotypes in gray terrace soil.

        Alam, Faridul,Bhuiyan, M A H,Alam, Sadia Sabrina,Waghmode, Tatoba R,Kim, Pil Joo,Lee, Yong Bok Japan Society for Bioscience, Biotechnology, and A 2015 Bioscience, Biotechnology, and Biochemistry Vol.79 No.10

        <P>Soybean plants require high amounts of nitrogen, which are mainly obtained from biological nitrogen fixation. A field experiment was conducted by soybean (Glycine max) genotypes, growing two varieties (Shohag and BARI Soybean6) and two advanced lines (MTD10 and BGM02026) of soybean with or without Rhizobium sp. BARIRGm901 inoculation. Soybean plants of all genotypes inoculated with Rhizobium sp. BARIRGm901 produced greater nodule numbers, nodule weight, shoot and root biomass, and plant height than non-inoculated plants. Similarly, inoculated plants showed enhanced activity of nitrogenase (NA) enzyme, contributing to higher nitrogen fixation and assimilation, compared to non-inoculated soybean plants in both years. Plants inoculated with Rhizobium sp. BARIRGm901 also showed higher pod, stover, and seed yield than non-inoculated plants. Therefore, Rhizobium sp. BARIRGm901 established an effective symbiotic relationship with a range of soybean genotypes and thus increased the nodulation, growth, and yield of soybean grown in gray terrace soils in Bangladesh.</P>

      • KCI등재

        A Randomized single blind controlled clinical trial on safety and efficacy of a Unani formulation (Itrifal-e-Sagheer) in dyslipidemia

        Alam, Sazid,Alam, MD Anzar,Quamri, MA,Sofi, Ghulamuddin,Khan, Mohd. Qudratullah,Ansari, Shabnam Cellmed Orthocellular Medicine and Pharmaceutical 2020 셀메드 (CellMed) Vol.10 No.1

        Aim:'Itrifal-e-Sagheer', a compound Unani formulation has been indicated in disease conditions simulating dyslipidemia. The present study was done to substantiate the efficacy of 'Itrifal-e-Sagheer' in dyslipidemia on scientific parameters. Materials and methods: A randomized, single blind, controlled, clinical trial was carried out on 30 patients of dyslipidemia who were randomly allocated into test (n = 15) or control (n = 15) groups. The test drug, Itrifal-e-Sagheer and control drug, Abana® were given to respective group for 45 days along with lifestyle modification. Results: The test drug significantly alleviated the symptoms of subjective parameters (palpitation, breathlessness and weight gain) (p<0.05). There was statistically significant reduction in lipid profile of the patients in test group (p<0.05) than control drug treatment. Conclusion: The study evidenced that Itrifal-e-Sagheer is potentially effective and safe in the treatment of dyslipidemia. However, a multicentric study with robust study design is required to generalize the results.

      • SCISCIESCOPUS

        Edge-of-things computing framework for cost-effective provisioning of healthcare data

        Alam, Md. Golam Rabiul,Munir, Md. Shirajum,Uddin, Md. Zia,Alam, Mohammed Shamsul,Dang, Tri Nguyen,Hong, Choong Seon Elsevier 2019 Journal of parallel and distributed computing Vol.123 No.-

        <P><B>Abstract</B></P> <P>Edge-of-Things (EoT)-based healthcare services are forthcoming patient-care amenities related to autonomic and persuasive healthcare, where an EoT broker usually works as a middleman between the Healthcare Service Consumers (HSC) and Computing Service Providers (CSP). The computing service providers are the edge computing service providers (ECSP) and cloud computing service provider (CCSP). Sensor observations from a patient’s body area networks (BAN) and patients’ medical and genetic historical data are very sensitive and have a high degree of interdependency. It follows that EoT based patient monitoring systems or applications are tightly coupled and require obstinate synchronization. Therefore, this paper proposes a portfolio optimization solution for the selection of virtual machines (VMs) of edge and/or cloud computing service providers. The dynamic pricing for an EoT computation service is considered by the EoT broker for optimal VM provisioning in an EoT environment. The proposed portfolio optimization solution is compared with the traditional certainty equivalent approach. As the portfolio optimization is a centralized solution approach, this paper also proposes an alternating direction method of multipliers (ADMM) based distributed provisioning method for the healthcare data in the EoT computing environment. A comparative study shows the cost-effective provisioning for the healthcare data through portfolio optimization and ADMM methods over the traditional certainty equivalent and greedy approach, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Edge-of-Things (EoT) computation framework for healthcare service provisioning. </LI> <LI> Portfolio optimization approach for cost-effective healthcare data provisioning. </LI> <LI> Alternating direction method of multipliers (ADMM) for healthcare data offloading. </LI> </UL> </P>

      • Antioxidant and hepatoprotective action of the crude ethanolic extract of the flowering top of Rosa damascena

        Alam, MA,Nyeem, MAB,Awal, MA,Mostofa, M,Alam, MS,Subhan, N,Rahman, M Mostafizur Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.2

        The hepatoprotective activity of the alcoholic extract of Rosa damascena was studied against paracetamol induced acute hepatotoxicity in rats. Liver damage was assessed by estimating serum enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and histopathology of liver tissue. Pre- and post-treatment with ethanolic extracts showed a dose-dependent reduction of paracetamol induced elevated serum levels of enzyme activity. The mechanism underlying the protective effects was assayed in vitro and the R. damascena extracts displayed dosedependent free radical activity using DPPH ($IC_{50}=162.525\;{\mu}g/ml$) and TBA method. The hepatoprotective action was confirmed by histopathological observation. The ethanolic extracts reversed paracetamol induced liver injury. These results suggest that the hepatoprotective effects of R. damascena extracts are related to its antioxidative activity.

      • SCIESCOPUS

        Flow interference between two tripped cylinders

        Alam, Md. Mahbub,Kim, Sangil,Maiti, Dilip Kumar Techno-Press 2016 Wind and Structures, An International Journal (WAS Vol.23 No.2

        Flow interference is investigated between two tripped cylinders of identical diameter D at stagger angle ${\alpha}=0^{\circ}{\sim}180^{\circ}$ and gap spacing ratio $P^*$ (= P/D) = 0.1 ~ 5, where ${\alpha}$ is the angle between the freestream velocity and the line connecting the cylinder centers, and P is the gap width between the cylinders. Two tripwires, each of diameter 0.1D, were attached on each cylinder at azimuthal angle ${\beta}={\pm}30^{\circ}$, respectively. Time-mean drag coefficient ($C_D$) and fluctuating drag ($C_{Df}$) and lift ($C_{Lf}$) coefficients on the two tripped cylinders were measured and compared with those on plain cylinders. We also conducted surface pressure measurements to assimilate the fluid dynamics around the cylinders. $C_D$, $C_{Df}$ and $C_{Lf}$ all for the plain cylinders are strong function of ${\alpha}$ and $P^*$ due to strong mutual interference between the cylinders, connected to six interactions (Alam and Meyer 2011), namely boundary layer and cylinder, shear-layer/wake and cylinder, shear layer and shear layer, vortex and cylinder, vortex and shear layer, and vortex and vortex interactions. $C_D$, $C_{Df}$ and $C_{Lf}$ are very large for vortex and cylinder, vortex and shear layer, and vortex and vortex interactions, i.e., the interactions where vortex is involved. On the other hand, the interference as well as the strong interactions involving vortices is suppressed for the tripped cylinders, resulting in insignificant variations in $C_D$, $C_{Df}$ and $C_{Lf}$ with ${\alpha}$ and $P^*$. In most of the (${\alpha}$, $P^*$ ) region, the suppressions in $C_D$, $C_{Df}$ and $C_{Lf}$ are about 58%, 65% and 85%, respectively, with maximum suppressions 60%, 80% and 90%.

      • Anti-inflammatory and anti-noceceptive action of the crude extracts of Costus specious on rodents

        Alam, Ashraful,Subhan, Nusrat,Awal, Abdul,Alam, Shohidul,Akramudau, Kazi Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

        The effect of alcoholic extracts of Costus specious (Family: Zingiberaceae) was evaluated in experimental models of pain and inflammation. Oral administration of 100, 200 and 300 mg/kg of C. specious extracts were used for the above study. Crude extracts of C. specious (300 mg/kg dose) showed maximum time needed for the response against thermal stimuli ($7.242\;{\pm}\;0.532\;s$) which is comparable to diclofenac sodium ($8.471\;{\pm}\;0.25\;s$) in the hot plate test. The MPH (Maximum Possible Analgesia) has been found to be 14.285 for 300 mg/kg dose of the crude extract while the MPH for diclofenac was 15.857 after 60 min of administration in the hot tail-flick method. The crude extract at 300 and 200 mg/kg doses showed significant reduction in acetic acid induced writhings in mice with a maximum effect of 59.661% reduction at 300 mg/kg dose which is comparable to standard diclofenac sodium (73.4%). Alcoholic extract of C. specious showed significant inhibition in serotonin and egg albumin induced hind paw oedema in rats at 100, 200 and 300 mg/kg of the crude extracts respectively (Serotonin induced edema 44.22; 53.75; 58.51%; egg albumin induced edema - 41.317; 53.892; 59.880% inhibition after 4 h respectively). The antiinflammatory effects showed by the extract were comparable to that of standard indomethacin 5 mg/kg (Serotonin induced edema 77.56%; egg albumin induced edema 77.844% inhibition after 4 h). These results suggest that the extract possesses both the anti-inflammatory and analgesic activity on mice and rat model.

      • Diagnostic Accuracy of Ultrasonography in Differentiating Benign and Malignant Thyroid Nodules Using Fine Needle Aspiration Cytology as the Reference Standard

        Alam, Tariq,Khattak, Yasir Jamil,Beg, Madiha,Raouf, Abdul,Azeemuddin, Muhammad,Khan, Asif Alam Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

        Background: In Pakistan thyroid cancer is responsible for 1.2% cases of all malignant tumors. Ultrasonography (US) is helpful in detecting cancerous thyroid nodules on basis of different features like echogenicity, margins, microcalcifications, size, shape and abnormal neck lymph nodes. We therefore aimed to calculate diagnostic accuracy of ultrasound in detection of carcinoma in thyroid nodules taking fine needle aspiration cytology as the reference standard. Materials and Methods: A cross-sectional analytical study was designed to prospectively collect data from December 2010 till December 2012 from the Department of Radiology in Aga Khan University Hospital, Karachi, Pakistan. A total of 100 patients of both genders were enrolled after informed consent via applying non-probability consecutive sampling technique. Patients referred to Radiology department of Aga Khan University to perform thyroid ultrasound followed by fine-needle aspiration cytology of thyroid nodules were included. They were excluded if proven for thyroid malignancy or if their US or FNAC was conducted outside our institution. Results: The subjects comprised 76 (76%) females and 24 males. Mean age was $41.8{\pm}SD$ 12.3 years. Sensitivity and specificity with 95%CI of ultrasound in differentiating malignant thyroid nodule from benign thyroid nodule calculated to be 91.7% (95%CI, 0.72-0.98) and 78.94% (0.68-0.87) respectively. Reported positive predictive value and negative PV were 57.9% (0.41-0.73) and 96.8% (0.88-0.99) and overall accuracy was 82%. Likelihood ratio (LR) positive was computed to be 4.3 and LR negative was 0.1. Conclusions: Ultrasonography has a high diagnostic accuracy in detecting malignancy in thyroid nodules on the basis of features like echogenicity, margins, micro calcifications and shape.

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