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        Short Communications : Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

        ( Md. Siddiqur Rahman ),( Amitavo Chakrabartty ),( Taohidul Islam ),( Roma Rani Sarker ),( Me Alam1,Muhammad Jasim Uddin ),( Laila Akther ),( Hee Jong Song ) 한국가축위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.2

        Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise seroprevalence was found 3.0% in 2∼3 years age group and 2.0% in 4∼8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

      • KCI등재

        Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

        Rahman, Md. Siddiqur,Chakrabartty, Amitavo,Islam, Md. Taohidul,Sarker, Roma Rani,Alam, M.E.,Uddin, Muhammad Jasim,Akther, Laila,Song, Hee-Jong The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.2

        Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

      • KCI등재

        Prevalence of Feline Leukemia Virus Infection in Cats in Bangladesh

        Siddiqur Rahman,Salauddin Bhuiyan,Taohidul Islam,Azimun Nahar,Roma Rani Sarker,Emtiaj Alam,Amitavo Chakrabarty,Abu Sayed Sarker,Laila Akhter,채준석 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.1

        Feline leukemia virus (FeLV) is a retrovirus that represents one of the most common and important infectiousdiseases of cats worldwide and it is responsible for more deaths among cats than any other infectious diseases. Prevalence data are necessary to define prophylactic, management and therapeutic measures for stray, feral and ownedcats which are lacking in Bangladesh. The study was carried out to determine the prevalence of FeLV infection inMymensingh district of Bangladesh using RapiGEN® FeLV Ag Test Kit (RapiGEN® Inc., Republic of Korea), a rapidone-step immunochromatographic assay. Blood samples from total 130 cats (23 owned cats and 107 unowned cats)were collected and tested following the manufacturer’s instruction. An overall prevalence of FeLV infection was 1.54%(2/130). Prevalence was found 1.79% (2/112) on Day 0-up to one year aged cats (young) but no positive case wasfound in above 1 year (Adult) aged group. In male and female cats, the prevalence was 1.72% (1/58) and 1.39%(1/72), respectively. In un-owned cats the prevalence was 1.87%. Positive cases to FeLV were found only in clinicallysick cats. No significant relationship was found according to age, sex, ownership status and health status. To the bestof our knowledge this is the first report of the prevalence of FeLV infection in Bangladesh using RapiGEN® FeLVtest kits which is very much effective because it is easy to apply, less expensive and quick screening of such typeof infection.

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