Effect of Amino Acid Substitution on the Subtype Specificity of preS2 Epitope in Hepatitis B Surface Antigen

Aeree Moon,Ki Sun Kwon,Myeong Hee Yu 생화학분자생물학회 1994 BMB Reports Vol.27 No.1

Antigenicity of the preS2 region of hepatitis B surface antigen was characterized by site-directed mutagenesis. Studies of serial deletions located the antigenic epitope between amino acid residues 132 and 143. Differences in the amino acid sequence between the adr and adw2 subtypes occur at residues 132 and 141. Amino acid substitutions at residue 132 in the adr sequence did not affect antigenicity of the preS2 to the monoclonal antibody H8, which is specific to the odr subtype preS2 sequence. However, substitutions at residue 141 did lead to a loss of antigenicity. A change of amino acid residue 141 from leucine to phenylalanine in the preS2 sequence of the adw2 subtype was sufficient for the sequence to be recognized by the monoclonal antibody H8. These results indicate that a single amino acid, Phe^(141), in the preS2 epitope is responsible for determining the subtype specificity recognized by the monoclonal antibody H8.

음주장소규제와 관련한 지자체 조례, 법 및 금연구역 분석

손애리(Aeree Sohn),서미경(MeeKyung Suh),문제은(Jei Eun Moon),장사랑(Sarang Jang) 한국중독정신의학회 2021 중독정신의학 Vol.25 No.2

Objectives: The objective of this study was to develop effective standard ordinances for the designation and management of alcohol prohibited zones in public places. Methods : To investigate public drinking regulation ordinances in Korea, we analyzed places where drinking should be controlled or prohibited, contents of regulations, laws related to drinking place regulation, and nonsmoking areas. Results : Results of the analysis of existing local government ordinances on alcohol-regulated places were as follows. A total of 91 local government ordinances specified places where drinking was prohibited or restricted. In these ordinances, restricted places were divided into external places where many people would gather and facilities for minors that would restrict access to alcohol according to age. Conclusion : To promote health, the designation of prohibition zones must include the protection of vulnerable members of the society whose health is at risk, sports facilities that must maintain a healthy environment, hospitals, and clinics. In addition, a unified administrative system for prohibition management should be considered.

The Effect of Glycyrrhizae Radix on the Metabolism of Acetaminophen

Kim, Hyo Jung,Moon, Aeree,Oh, Jee Young,Kim, Seung Hee,Lee, Song Deuck 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.2

The effects of Glycyrrhizae Radix (GR) on the metabolism of acetaminophen (AA) were examined in male Sprague-Dawley rats. The methanol extract of GR (500 mg/kg) was administered orally to rats for 6 days. AA and its metabolites excreted in bile, urine and blood within 120 min after dosing of AA (150 mg/kg, i.v.) were assayed by HPLC. Treatment of rats with the methanol extract of GR significantly increased the cumulative biliary excretion of AA-glucuronide (156% of the control) and decreased that of AA-sulfate (63% of the control). The cumulative urinary excretion of AA-glucuronide was also significantly increased to 132% of the control. GR treatment significantly increased total (biliary plus urinary) excretion of AA-glucuronide (172% of the control) without influencing thioether and sulfate conjugates of AA. The results clearly show that GR enhances UDP-glucuronosyl transferase-mediated detoxication of AA, but may not influence sulfotransferase-mediated and cytochrome P-450-mediated metabolites formation.

Induction of Ornithine Decarboxylase and Tumor Promotion by N-Methy-N'-Nitro-N-Nitrosoguanidine, Sokium Chloride, and Dimethyl Itaconate

Choi, Kwang Sik,Hwang, Moon Ok,Kim, Dae Joong,Lee, Chong Kyo,Moon, Aeree,Han, Beom Seok 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.2

The possible tumor-promoting activities of sodium chloride (NaCl) and dimethyl itaconate (DMI), one of the quinone reductase inducers, were examined on stomach of male Wistar rats treated with N-methylN-nitro-N-nitrosoguanidine (MNNG). Administrations of NaCl and DMI after the initiation by MNNG resulted in various sized masses in the rat forestomach. Histopathologic studies showed that the combination of NaCl and DMI made an enhancing effect on the MNNG-induced carcinogenesis, resulting in papilloma in 5 weeks and squamous cell carcinoma in 20 weeks in submucosal area of forestomach. We also used an in vivo shortterm method for evaluating possible tumor-promoting activity with ornithine decarboxylase (ODC) as a marker. The markable inductions of the ODC activities by MNNG, NaCl, and DMI were found in the pyloric mucosa of rat stomach in time-dependent manners. A single administration of MNNG induced ODC activity up to 288 pmol CO₂/hr/mg protein at 24 hr after the administration. NaCl caused induction of ODC with a maximum of 179 pmol CO-2/hr/mg protein at 8 hr after the administration. ODC was induced up to 539 pmol CO₂/hr/mg protein at 16 hr after the administration of DMI. Additional treatment of NaCl and NaCl plus DMI caused 2 fold and 7 fold increases, respectively, in the ODC activity of the MNNG-alone group at 24 hr after the administration. These results suggest that NaCl and DMI have promoting activities in the rat gastric carcinogenesis initiated by MNNG.

Secretory Profiles and Wound Healing Effects of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

Yoon, Byung Sun,Moon, Jai-Hee,Jun, Eun Kyoung,Kim, Jonggun,Maeng, Isaac,Kim, Jun Sung,Lee, Jung Han,Baik, Cheong Soon,Kim, Aeree,Cho, Kyoung Shik,Lee, Jang Ho,Lee, Hwang Heui,Whang, Kwang Youn,You, Se Mary Ann Liebert 2010 STEM CELLS AND DEVELOPMENT Vol.19 No.6

<P>Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD44 and differentiated into adipocytes, osteoblasts, and chondrocytes when exposed to the appropriate differentiation media. In addition, AF-MSC-conditioned media (AF-MSC-CM) significantly enhanced proliferation of dermal fibroblasts. Antibody-based protein array and enzyme-linked immunosorbent assay (ELISA) indicated that AF-MSC-CM contains various cytokines and chemokines that are known to be important in normal wound healing, including IL-8, IL-6, TGF-beta, TNFRI, VEGF, and EGF. Application of AF-MSC-CM significantly enhanced wound healing by dermal fibroblasts via the TGF-beta/SMAD2 pathway. Levels of p-SMAD2 were increased by AF-MSC-CM, and both the increase in p-SMAD2 and migration of dermal fibroblasts were blocked by inhibiting the TGF-beta/SMAD2 pathway. Moreover, in a mouse excisional wound model, AF-MSC-CM accelerated wound healing. These data provide the first evidence of the potential for AF-MSC-CM in the treatment of skin wounds.</P>

Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells

Kim, Bona,Yoon, Byung Sun,Moon, Jai-Hee,Kim, Jonggun,Jun, Eun Kyoung,Lee, Jung Han,Kim, Jun Sung,Baik, Cheong Soon,Kim, Aeree,Whang, Kwang Youn,You, Seungkwon Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.1

Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1: MSC expansion). hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes, osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2: mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled, 3-dimensional islet cell-like clusters that express multiple genes related to pancreatic ${\beta}$-cell development and function (stage 3: IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.

위탁형 대안학교를 선택한 학교폭력피해 학부모 경험에 관한 현상학적 연구

김현희(Kim Hyun hee),천성문(Cheon Seong moon),김애리(Kim Aeree) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.22

The purpose of this study was to deal with school violence and to provide the basic data on children’s school adaptation in terms of in-depth understanding of parents’ experiences about their children attending consigned alternative schools after school violence, In order to reach the goals, the interviews were conducted with 6 parents who had their children attending consigned alternative schools. The data were analyzed through Giorgi’s phenomenology. The results show that 9 categories and 28 components were extracted. Parents who have sent school violence victims to a foster alternative school experience psychological trauma due to their child s school violence. Parents send their children to a foster school. The wounds are getting deeper because of the divergent reactions, gaze, and wandering children around. However, there is a positive change in the child by allowing him / her to afford it through a program of a foster alternative school that is more interested in children and offers a variety of experience opportunities. Parents were found to have experience of recovering from psychological trauma caused by their child s school violence. Based on these findings, the pedagogical implications will be provided for parents and children who cannot adapt a school after school violence. In addition, the suggestions for future studies exploring the prevention of school violence will be offered. 본 연구의 목적은 학교폭력피해를 경험한 자녀를 위탁형 대안학교로 보낸 학부모의 경험이 어떠하며, 그 경험의 구조가 무엇인지를 살펴봄으로써 그들의 경험을 심층적으로 이해하는 데 있다. 이를 위해 자녀가 6개월 이상 위탁형 대안학교에 재학 중인 학부모 6명을 대상으로 심층면담을 실시하였으며, 현상학적 연구방법 중 Giorgi의 분석방법을 사용하였다. 분석을 통해 545개의 의미단위를 도출, 156개의 의미단위로 요약하고, 최종 9개의 구성요소와 28개의 하위구성요소를 도출하였다. 분석결과, 학교폭력피해를 경험한 자녀를 위탁형 대안학교로 보낸 학부모의 경험은 위탁 이전, 위탁 중, 앞으로의 삶이라는 시간적 구조 속에서 ‘자녀가 학교폭력의 희생자가 됨,’ ‘학교폭력 사건 후 남은 것은 후회와 상처뿐임,’ ‘지푸라기라도 잡는 심정으로 대안학교를 찾음,’ ‘어쩔 수 없는 선택 후 흔들리는 마음,’ ‘추락하는 자녀의 손을 잡음,’ ‘자녀가 긍정적으로 변화되어 편안해짐,’ ‘마음이 긍정적으로 변화됨,’ ‘자녀의 미래를 걱정함,’ ‘대안학교와 함께 나아가고 싶은 바램이 생김’의 과정으로 해석되었다. 이러한 결과를 바탕으로 본 연구의 의의와 제한점 및 제언이 논의되었다.

Eccrine Porocarcinoma: A Multicenter Retrospective Study with Review of the Literatures Reported in Korea

( Hee Joo Kim ),( Anna Kim ),( Kyung-chul Moon ),( Soo Hong Seo ),( Il-hwan Kim ),( Aeree Kim ),( Yoo Sang Baek ) 대한피부과학회 2020 Annals of Dermatology Vol.32 No.3

Background: Eccrine porocarcinoma (EPC) is a rare malignant cutaneous adnexal tumor. Other than several scattered case reports, no comprehensive review on EPC has been conducted in Korea. Objective: To clinicopathologically review all EPC cases from our institutions as well as those reported in Korea. Methods: Medical records and histopathological slides of EPC cases in the skin biopsy registries of our institutions were retrospectively reviewed. Additionally, EPC cases reported in Korea before June 2019 were retrieved by searching the PubMed, KoMCI, KoreaMed, and KMbase databases. Results: Nine EPC cases from our institutions were included in the study. In addition, 27 reports of 28 patients with EPC were reported in Korea. A total of 37 patients with EPC were identified, consisting of 19 males (male:female ratio, 1.06:1; mean age at diagnosis, 65.6 years). The most common site of primary tumor was the head and neck (29.7%). Wide excision was the most common (78.4%) treatment method. Initial metastasis work-up imaging studies were performed in 18 patients (48.6%), and metastasis was confirmed in eight patients (21.6%). Conclusion: EPC is a rare cutaneous carcinoma in Korea. EPC usually affects elderly patients, with no sexual predilection. Due to possible metastasis, careful diagnosis and appropriate metastasis workups are warranted in EPC. (Ann Dermatol 32(3) 223∼229, 2020)