Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells

Kim, Bona,Yoon, Byung Sun,Moon, Jai-Hee,Kim, Jonggun,Jun, Eun Kyoung,Lee, Jung Han,Kim, Jun Sung,Baik, Cheong Soon,Kim, Aeree,Whang, Kwang Youn,You, Seungkwon Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.1

Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1: MSC expansion). hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes, osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2: mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled, 3-dimensional islet cell-like clusters that express multiple genes related to pancreatic ${\beta}$-cell development and function (stage 3: IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.

Simple Approach to Superhydrophobic Nanostructured Al for Practical Antifrosting Application Based on Enhanced Self-propelled Jumping Droplets

Kim, Aeree,Lee, Chan,Kim, Hyungmo,Kim, Joonwon American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.13

<P>Frost formation can cause operational difficulty and efficiency loss for many facilities such as aircraft, wind turbines, and outdoor heat exchangers. Self-propelled jumping by condensate droplets on superhydrophobic surfaces delays frost formation, so many attempts have been made to exploit this phenomenon. However, practical application of this phenomenon is currently unfeasible because many processes to fabricate the superhydrophobic surfaces are inefficient and because self-propelled jumping is difficult to be achieved in a humid and low-temperature environment because superhydrophobicity is degraded in these conditions. Here, we achieved significantly effective anti-icing superhydrophobic aluminum. Its extremely low adhesive properties allow self-propelled jumping under highly supersaturated conditions of high humidity or low surface temperature. As a result, this surface helps retard frost formation at that condition. The aluminum was made superhydrophobic by a simple and cost-effective process that is adaptable to any shape. Therefore, it has promise for use in practical and industrial applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-13/acsami.5b00292/production/images/medium/am-2015-00292x_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b00292'>ACS Electronic Supporting Info</A></P>

COVID-19: Korea’s Experience -From the Perspective of Health Communication and Crisis Management-

Aeree Sohn,Myung-Il, Choi,Hyegyu Lee,Daewook Kim,HAN SUK JUNG,Jang Sarang,Yoon, Nan-He,Choi Young-Joon,Kim In-yeong 한국국제협력단 2020 연구보고서 Vol.- No.-

Durable, scalable, and tunable omniphobicity on stainless steel mesh for separation of low surface tension liquid mixtures

Kim, Aeree,Lee, Chan,Kim, Joonwon Elsevier 2018 Surface & coatings technology Vol.344 No.-

<P><B>Abstract</B></P> <P>Surface repellency to high and low surface tension (LST) liquids has a wide range of commercial applications such as non-fouling, self-cleaning and separation of liquids. However, little research has been achieved to control surface repellency to LST liquids due to difficulty of developing re-entrant surface curvatures. Here, we report an effective method to control surface repellency of stainless steel meshes for liquids that have LST. We used electrochemical etching followed by hydrophobic coating where adjusting the voltage and duration of electrochemical etching controllably cover the mesh surfaces with nanoscale re-entrant structures. Variation in re-entrant surface curvatures determines the mesh's sensitivity to liquid-vapor surface tension, and changed the apparent contact angles and sliding angles for LST liquids. The difference in sensitivity of surface tension of mesh was exploited to rapidly separate liquids in binary mixtures of general solvents. The meshes achieve fast separation, and can be reused. This method to provide omniphobicity is compatible with large-scale production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We report an effective method for tunable omniphobicity on 304 stainless steel mesh. </LI> <LI> The prepared mesh has mechanically and chemically durable omniphobicity. </LI> <LI> Omniphobicity can be tuned by variation in coverage of re-entrant structures. </LI> <LI> This method can control the mesh's sensitivity to γ<SUB>lv</SUB>, and the CAs for LST liquids. </LI> <LI> Tunable wettability of mesh can separate diverse liquid mixtures. </LI> </UL> </P>

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma.

Kim, Chan Yong,Jung, Woon Yong,Lee, Hyun Joo,Kim, Han Kyeom,Kim, Aeree,Shin, Bong Kyung National Hellenic Research Foundation 2012 Oncology reports Vol.27 No.3

<P>The study of tumor biomarkers was gradually facilitated by the adoption of proteomic strategies due to less invasiveness and higher sensitivity. Colorectal cancer is one of the most commonly occurring cancers worldwide and its incidence has markedly increased in Korea. While the adoption of proteomic strategies facilitated the study of tumor biomarkers, to date, no common agreement has been derived from proteomic investigations regarding tumor markers of colorectal cancer. This study was designed to find molecules differentially expressed in colorectal cancer compared to non-tumor mucosa. Four colorectal adenocarcinoma and corresponding non-tumor tissue samples were analyzed to find previously unknown proteins via two-dimensional electrophoresis and MALDI-TOF/MS spectrometry. Western blot assays and tissue microarray (TMA) immunohistochemistry were performed to validate the identified proteins. Among the twelve up-regulated and one down-regulated proteins identified, moesin, cytokeratin (KRT) 17 and carbonic anhydrase??I were validated by western blot analysis and/or immunohistochemistry. On immunohistochemistry, both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. Both moesin and KRT17 were not expressed in normal colorectal epithelium. These two proteins may play a role in cancer invasion and/or metastasis in colorectal carcinoma, and could be candidate biomarkers for the diagnosis and prognosis of colorectal cancer.</P>

The Effect of Glycyrrhizae Radix on the Metabolism of Acetaminophen

Kim, Hyo Jung,Moon, Aeree,Oh, Jee Young,Kim, Seung Hee,Lee, Song Deuck 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.2

The effects of Glycyrrhizae Radix (GR) on the metabolism of acetaminophen (AA) were examined in male Sprague-Dawley rats. The methanol extract of GR (500 mg/kg) was administered orally to rats for 6 days. AA and its metabolites excreted in bile, urine and blood within 120 min after dosing of AA (150 mg/kg, i.v.) were assayed by HPLC. Treatment of rats with the methanol extract of GR significantly increased the cumulative biliary excretion of AA-glucuronide (156% of the control) and decreased that of AA-sulfate (63% of the control). The cumulative urinary excretion of AA-glucuronide was also significantly increased to 132% of the control. GR treatment significantly increased total (biliary plus urinary) excretion of AA-glucuronide (172% of the control) without influencing thioether and sulfate conjugates of AA. The results clearly show that GR enhances UDP-glucuronosyl transferase-mediated detoxication of AA, but may not influence sulfotransferase-mediated and cytochrome P-450-mediated metabolites formation.

Anticancer effect of metformin on estrogen receptor-positive and tamoxifen-resistant breast cancer cell lines

KIM, JINKYOUNG,LEE, JIYUN,JANG, SOON YOUNG,KIM, CHUNGYEUL,CHOI, YOOJIN,KIM, AEREE NATIONAL HELLENIC RESEARCH FOUNDATION 2016 ONCOLOGY REPORTS Vol.35 No.5

<P>Acquisition of tamoxifen resistance (TR) during anti-estrogenic therapy using tamoxifen is a major obstacle in the treatment of estrogen receptor (ER)-positive breast cancer. As a biguanide derivative, metformin is commonly used to treat type II diabetes. It has recently emerged as a potential anticancer agent. The objective of the present study was to investigate the anticancer activity of metformin in relation to ERα expression and its signaling pathway in ERα-positive MCF-7 and MDA-MB-361 breast cancer cells as well as TR MCF-7 breast cancer cells. Metformin inhibited both protein and mRNA levels of ERα in the presence or absence of estrogen (E2) in the MCF-7, TR MCF-7 and MDA-MB-361 cells. Metformin repressed E2-inducible estrogen response element (ERE) luciferase activity, protein levels and mRNA levels of E2/ERα-regulated genes [including c-Myc, cyclin D1, progesterone receptor (PR) and pS2] to a greater degree than tamoxifen, resulting in inhibition of cell proliferation of MCF-7, TR MCF-7 and MDA-MB-361 cells. Collectively, our results suggest that one of the anticancer mechanisms of metformin could be attributable to the repression of expression and transcriptional activity of ERα. Metformin may be a good therapeutic agent for treating ERα-positive breast cancer by inhibiting the expression and function of ERα. In addition, metformin may be useful to treat tamoxifen-resistant breast cancer.</P>

A STUDY OF DWARF GALAXIES EMBEDDED IN A LARGE-SCALE Hɪ RING IN THE LEO I GROUP

KIM, MYO JIN,CHUNG, AEREE,LEE, JONG CHUL,LIM, SUNGSOON,KIM, MINJIN,KO, JONGWAN,LEE, JOON HYEOP,YANG, SOUNG-CHUL,LEE, HYE-RAN The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

A large-scale neutral hydrogen ($H\small{I}$) ring serendipitously found in the Leo I galaxy group is 200 kpc in diameter with $M_{H\small{I}}{\sim}1.67{\times}10^9M_{\odot}$, unique in size in the Local Universe. It is still under debate where this $H\small{I}$ ring originated - whether it has formed out of the gas remaining after the formation of a galaxy group (primordial origin) or been stripped during galaxy-galaxy interactions (tidal origin). We are investigating the optical and $H\small{I}$ gas properties of the dwarf galaxies located within the gas ring in order to probe its formation mechanism. In this work, we present the photometric properties of the dwarfs inside the ring using the CFHT MegaCam $u^{\ast}$, $g^{\prime}$, $r^{\prime}$ and $i^{\prime}$-band data. We discuss the origin of the gas ring based on the stellar age and metal abundance of dwarf galaxies contained within it.

Formulation of Conductive Filament Composited of Thermoplastic with Carbon Black for a Simple 3D Printing Electrical Device

Jo, Aeree,Chae, Heedo,Kim, Yongjun,Kim, Heeju,Paek, Seunghwi,Soum, Veasna,Jang, Wonhyeong,Ryu, Soo Ryeon,Kwon, Oh-Sun,Shin, Kwanwoo American Scientific Publishers 2016 Journal of nanoscience and nanotechnology Vol.16 No.8

<P>The thermoplastic- based conductive carbon black (CB) composite filament was fabricated by extrusion method and explored to manufacture an electrical device by three- dimensional (3D) printing. The achieved electrical line resistance was 150 Omega/cm for a cylindrical wire- shape filament which was extruded through a 1.75 mm diameter nozzle at 205 degrees C by loading pre- composited pellets, which were formulated with thermoplastic acrylonitrile butadiene styrene (ABS) and 20 vol% CB filler in chloroform solvent without any other additive. Conductivity was still lower than those of metal conductors. It has been successfully applied for printing a circuit line having internal resistance. Besides of the circuit line, other circuit elements, such as a switch and a base board, have printed only relying on 3D printing, demonstrating an excellent 3D printing of simple electrical circuit, successfully. Especially, an elastic ABS plastic membrane spring printed and was used for making a power switch with a conducting power contact pad which was also printed with the conductive filament. Characterization of the ABS/CB composite filament has been performed by using scanning electron microscope, differential scanning calorimeter and Fourier transform infrared spectroscopy.</P>

수면다원검사를 통해 분석한 소아 뇌전증환자의 수면장애

김애리(Aeree Kim),이지원(Jiwon Lee),주은연(Eun Yeon Joo),이지훈(Jeehun Lee),이문향(Munhyang Lee) 대한소아신경학회 2016 대한소아신경학회지 Vol.24 No.1

Purpose: The objectives of this study were to identify the characteristics of sleep disorders in children with epilepsy complaining of sleep problems, and to find out sleep-related factors that influence on the prognosis of epilepsy. Methods: We retrospectively reviewed medical records and polysomnographic data of 94 children (mean age 13.7±3.8 years), who visited our hospital for epilepsy between March 2001 and December 2014. The patients were evaluated with polysomnography and a questionnaire about sleepiness. We compared clinical characteristics between patients with and without sleep disorders. We analyzed the differences of sleep parameters between the prognostic groups of epilepsy. Results: The patients enrolled in this study complained of various sleep problems, such as snoring, apnea, mouth-breathing, excessive daytime sleepiness, and insomnia. Sleep disorders were diagnosed in 46 patients (48.9%). Obstructive sleep apnea was the most common sleep disorder (n=26), followed by narcolepsy (n=8) and periodic limb movement disorder (n=8). Patients with poor prognosis showed decrease of stage 3-4 sleep (P=0.010). There was no significant correlation between the presence of sleep disorders and seizure control (P=0.053). Conclusion: Various sleep complaints and sleep disorders are commonly found in children with epilepsy. Polysomnographic analysis of patients with uncontrolled seizure indicates a decline in sleep quality. Screening sleep problems and performing diagnostic test with polysomnography in patients with sleep problems is recommended in pediatric patients with epilepsy.