Alteration in Phenolic Compounds and Antioxidant Activities of Aronia melanocarpa Ethanol Extracts following Fermentation Using Different Strains of Leuconostoc mesenteroides to Develop Natural Antibiotic Alternative

황주환,강주희,이기환,이재훈,이상무,김남형,김주영,김은중,Hwang, Joo Hwan,Kang, Ju Hui,Lee, Ki Hwan,Lee, Jae Hoon,Lee, Sang Moo,Kim, Nam Hyung,Kim, Joo Young,Kim, Eun Joong Korean Association of Organic Agriculture 2014 韓國有機農業學會誌 Vol.22 No.4

Antioxidant activity is important for reducing oxidative stress that causes various metabolic disorders. Metabolic disorders are highly related to loss of productivity in livestock. Therefore, development of effective antioxidant compounds originating from plants is important for organic agriculture. Phenolic compounds in edible plants are regarded as major components relevant to antioxidant activity. The present study investigated the changes in antioxidant activity and phenolic compound profiles of Aronia (Aronia meloncarpa) by fermentation using different strains of Leuconostoc mesenteroides. A total of 5 strains of L. mesenteroides were used as starter cultures and their ${\beta}$-glucosidase activities were measured. A total of 6 experiment runs were prepared, one for control (uninoculated) and the others (inoculated) for treatments. For biological activity, antioxidant and antibacterial activities were measured. For phenolic compound profiling, TLC and HPLC analysis were performed. The strains of KACC12313 and KACC12315 showed greater enzyme activity than others. Treatment with KCCM35046 showed strong and broad antibacterial activity against to Listeria monocytogenes. Treatments with KCCM35046 and KACC12315 showed the highest total polyphenol content. The highest antioxidant activity was found in KACC12315 treatment. No remarkable alteration was found in thin layer chromatography (TLC) analysis. In phenolic compound profiling analysis, KCCM35046 showed notable alteration in compound area ratio compared to others and also showed the highest caffeic acid content. In chlorogenic acid, treatments with KCCM35046 and KACC12315 showed great content than others. Treatment with KACC12315 showed the greatest content of trans-ferulic acid. As a result of relative performance indexing analysis, L. mesenteroides KCCM35046 and KACC12315 were selected as the best strain for the fermentation of Aronia.

논 토양에서 사일리지용 수수 × 수수 교잡종 재배시 화학비료와 발효 돈분 액비 혼용 시용이 생육특성 및 영양성분에 미치는 영향

황주환,이상무,Hwan, Hwang Joo,Lee, Sang Moo 한국초지조사료학회 2015 한국초지조사료학회지 Vol.35 No.4

본 실험은 논 토양에서 사일리지용 수수 ${\times}$ 수수 교잡종 재배시 화학비료와 발효 돈분 액비 혼용 시용이 생육특성 및 영양성분에 미치는 영향을 조사하고자 실시하였다. 실험설계는 화학비료 100% 처리구 (C), 화학비료 70% + 돈분액비 30% 처리구 (T1), 화학비료 50% + 돈분액비 50% 처리구 (T2), 화학비료 30% + 돈분액비 70% 처리구 (T3) 그리고 돈분액비 100% 처리구 (T4)로 한, 5처리 3반복 난괴법으로 배치하였다. 이때 돈분 액비 시용은 질소량만을 기준으로 하였다 (150 kg/ha). 초장, 엽장, 엽폭 그리고 경의 굵기는 T4구가 유의적으로 작게 나타났다 (p<0.05). 경의 경도는 돈분액비 시용 비율 낮고 화학비료 시용 비율이 높을수록 유의적으로 증가하였다 (p<0.05). 생초수량은 T2구가 높았던 반면 T3구가 낮게 나타났다 (p<0.05). 그러나 건물 수량 및 TDN 수량은 처리구들 간에 유의적인 차이를 보이지 않았다. 조단백질 함량은 T1구가 다른 구에 비하여 높게 나타났다 (p<0.05). 조지방 함량은 T1, T2, T3 그리고 T4 처리구들 간에는 유의적인 차이가 없었지만, C구와는 유의적인 차이를 보였다 (p<0.05). NDF와 조섬유 함량은 각각 T3구와 C구에서 높게 나타났다 (p<0.05). 그러나 ADF 함량은 처리구들 사이에 유의적인 차이를 보이지 않았다. 총무기물 함량은 T1 > T2 > T4 > T3 > C 구 순으로 높게 나타났다 (p<0.05). 유리당 함량은 T1과 C구가 다른 처리구에 비하여 유의적으로 높게 나타났다 (p<0.05). 이상의 결과를 종합해 볼 때, 돈분액비 시용은 화학비료에 비하여 수량성, 영양성분에 크게 떨어지지 않기 때문에 사일지용 수수 ${\times}$ 수수 교잡종 재배시 돈분액비와 화학비료를 혼용 시용 하거나 돈분액비 만 시용하여도 큰 무리가 없는 것으로 판단된다. This study was conducted to investigate the influence of the mixed application of chemical fertilizer (CF) with liquid swine manure (LSM) on the agronomic characteristics, dry matter yield, minerals, and free sugar in cultivating Sorghum ${\times}$ Sorghum Hybrid (SSH) on paddy soil. The field experiment was designed in a randomized block design with three replications and consisted of CF 100% (C), CF 70% + LSM 30% (T1), CF 50% + LSM 50% (T2), CF 30% + LSM 70% (T3), and LSM 100% treatment (T4). The application of LSM was based only on the nitrogen (150 kg/ha). Plant length, leaf length, leaf width and stem diameter were significantly the lower in T4 (p<0.05). Stem hardness increased significantly (p<0.05) as the LSM application rate decreased. Fresh yield was the highest in T2, whereas the lowest in T3 (p<0.05). However, dry matter yields and TDN yield did not show significant difference among treatments. Crude protein was the highest in T1 (p<0.05). Crude fat content did not significant differences between the T1, T2, T3 and T4, but C showed a significantly different (p<0.05). NDF and crude fiber were the highest in T3 and C, respectively (p<0.05). However, ADF did not show significant difference among treatments. Total mineral contents were higher in the order of T1> T2> T4> T3> C (p<0.05). Free sugar contents were significantly higher at T1 and C as compared to other treatments. The analysis of all the above results suggests that the application of liquid swine manure is very effective, considering the yield performance and the content of mineral and free sugar. In addition, liquid swine manure may be possible to grow SSH without chemical fertilizer.

잠복결핵감염 양성인 분진작업 근로자에서 활동성 결핵 발병률

황주환 ( Joo Hwan Hwang ) 한국산업보건학회 (구 한국산업위생학회) 2020 한국산업보건학회지 Vol.30 No.1

Objectives: Previous study has shown that the positive rate of latent tuberculosis infection (LTBI) among former workers in dusty environments was higher than that among high-risk groups of tuberculosis (TB). The objective of the present study was to identify the development of active TB among former workers in dusty environments diagnosed with LTBI. Methods: Between January 2015 and May 2017, 796 former workers in dusty environments who had been subjects of epidemiology research for work-related chronic obstructive pulmonary disease (COPD) had received the QuantiFERON-TB® Gold In-Tube (QFT-GIT) from the Institute of Occupation and Environment (IOE) under the Korea Workers’ Compensation and Welfare Service (KCOMWEL). Among them, 437 participants who received a health examination for work-related pneumoconiosis between January 2015 and December 2018 were selected as study subjects. Active TB was defined as a positive result for active PTB and non-tuberculosis mycobacteria infection in the result of the Pneumoconiosis Examination Council’s assessment by KCOMWEL. Results: A total of 437 subjects were followed up for 2.1 years. Four of them (4/437, 0.9%) developed active TB during the follow-up period. The attack rate of active TB among subjects who were diagnosed LTBI positive and those who were diagnosed LTBI negative were 0.9% (3/320) and 0.9% (1/115), respectively. Conclusions: Most previous studies reported that the attack rate of the development of active TB in subjects who had been diagnosed LTBI positive was higher than that among subjects who had been diagnosed LTBI negative. To the contrary, the present study found that the rate of developing active TB among former workers in dusty environments diagnosed as LTBI positive was not higher than that in those who were diagnosed LTBI negative.

도심지 중앙버스 전용차로의 프리캐스트 패널을 이용한 급속 포장기술

황주환(Ju-Hwan Hwang),안승주(Seung-Joo An),김재훈(Jae-Hoon Kim),한정헌(Jung-Hun Han) 한국콘크리트학회 2024 콘크리트학회지 Vol.36 No.1

고내구성능을 갖는 해저터널 방화문 설계방안 및 성능시험 연구

박상헌,황주환,최영환,안승주,유용호,Park, Sang-Heon,Hwang, Ju-Hwan,Choi, Young-Hwan,An, Sung-Joo,Yoo, Yong-Ho 한국터널지하공간학회 2018 한국터널지하공간학회논문집 Vol.20 No.2

In the tunnel of domestic high - speed railway, the main fire - fighting facility, fire - extinguishing passageway, is installed. However, due to the high pressure of the high - speed train, frequent breakage and maintenance are caused by strong shock and long - term vibration. In order to solve these problems, it is necessary to improve the fire door, but in Korea, it is installed by submitting a certificate by simple KS F 2296 performance test. At present, it is developed as a simple test certification by producing a real scale fireproof door without the theoretical examination in advance, so that a high cost for improvement is occurring in Korea. Therefore, through this study, structural analysis study which can preliminary structure review was carried out in order to design the refuge connection passage fire door and to improve the performance improvement. In order to secure the reliability of the result value, the official authentication test (KS F 2296) were compared. 국내 고속철도 터널에 주요 방재시설물인 피난연결통로 방화문이 설치되고 있으나, 고속으로 통행하는 열차의 고압력으로 인하여 방화문에 강한 충격 및 장기간 진동으로 인하여 잦은 파손 및 유지보수가 발생되고 있다. 이러한 문제를 해결하기 위해 방화문 개선이 필요하나, 국내에서는 단순 KS F 2296 성능시험에 의한 인증서를 제출하여 설치되고 있다. 현재 국내에서는 사전에 이론적인 검토 없이 실규모 방화문 제작에 의한 단순 시험인증 여부로 개발 되다보니, 개선을 위한 고비용이 발생되고 있는 실정으로 사전에 구조적인 안전을 확보할 수 있는 설계방안의 검토가 필요하다. 따라서 본 연구를 통하여 피난연결통로 방화문 설계 및 성능개선 효율화를 위해 사전 구조 검토가 가능한 구조해석 연구를 수행하였으며, 이러한 결과 값에 대한 신뢰성을 확보하기 위해 실규모 시작품 제작을 통한 공인 인증시험(KS F 2296)에서 발생된 결과 값을 비교검토 하였다.

콘크리트 포장 보수를 위한 조립식 포장 시험시공

황성재(Hwang Seong Jae),황주환(Hwang Ju Hwan),김성민(Kim Seong-Min),양성철(Yang Sungchul),박성기(Park Sung Ki),박원주(Park Won Joo) 대한토목학회 2010 대한토목학회 학술대회 Vol.2010 No.10

사료 내 엄나무 첨가 급여가 육계의 생산성 및 계육의 지방산 조성에 미치는 영향

이상무,황주환,김영직,Lee, Sang-Moo,Hwang, Joo-Hwan,Kim, Young-Jik 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.2

The effects of dietary supplementation with Kalopanax pictus branch, leaves, and extracts on performance, proximate composition, pH, total phenol and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, cholesterol and fatty acid composition of chicken meat. Broiler chicks were fed for 5 weeks with a diet containing 5% Kalopanax pictus branch (T1), 2% Kalopanax pictus leaves (T2), and 5% Kalopanax pictus extracts (60% leaves + 40% branch) (T3). T1 and T3 resulted in much better feed conversion than T2. In proximate composition, crude fat of chicken meat was increased, but not signi-ficantly (p>0.05) in Kalopanax pictus-supplemented diets than control diet. Total phenol contents and DPPH radical scavenging activity were significantly increased (p<0.05) by the dietary-supplementation with Kalopanax pictus branch and leaves compared to the control diet. Total and high density lipoprotein cholesterols of treatment groups were higher than the control group, and control group low density lipoprotein cholesterolwas higher than treatment groups (p<0.05). Especially, T3 was significantly (p<0.05) more effective in improving cholesterol compared to other treatment groups. The treatment groups (especially T3) showed significantly higher total fatty acid and unsaturated fatty acid compositions compare to the control group.

도심지 교차로 구간 조립식 콘크리트 포장 시공 및 초기 공용성 추적조사

오한진(Oh, Han Jin),황주환(Hwang, Ju Hwan),김성민(Kim, Seong-Min),이석근(Rhee, Suk-Keun),박원주(Park, Won Joo),박성기(Park, Sung Ki) 한국콘크리트학회 2011 한국콘크리트학회 학술대회 논문집 Vol.2011 No.5

논토양에 사일리지용 옥수수 재배시 품종별 생육특성 및 영양성분 비교 연구

김완수(Wan Su Kim),황주환(Joo Hwan Hwang),이재훈(Jae Hun Lee),김은중(Eun Joong Kim),전병태(Byong Tae Jeon),문상호(Sang Ho Moon),이상무(Sang Moo Lee) 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.1

본 연구는 벼 대체 사료작물인 사일리지용 옥수수를 논토양에 파종하여 조사료를 생산하는데 있어서 생육특성, 화학적 특성, 무기물함량 및 영양수량을 검토하여 논토양에 적합한 사일리지용 옥수수 품종을 선발하기 위하여 실시하였다. 실험설계는 11품종을 3반복으로 하여 5월 1일 파종하고 8월 24일 수확하였다. 그 결과를 보면 초장과 엽수에 있어서는 KPO가 다른 품종에 비하여 유의적으로 높게 나타났지만(P<0.01) 암이삭 길이와 둘레는 P32W86이 높게 나타났다. 경경도와 암이삭 수는 P32P75가, 착수고 및 녹색도에 있어서는 NC+7117이 높게 나타났다(P<0.01). 근계수와 당도에 있어서는 P3394가 높게, 엽장과 엽폭은 각각 P31P41과 P32T83이 높게 나타났다. 건물수량에 있어서는 P3156 (19,782 ㎏) > NC+7117 (18,708 ㎏) > P31N27 (18,093 ㎏) > KPO (17,772 ㎏) > P32K61 (17,649 ㎏/ha) 순으로 높게 나타났다(P<0.01). 영양성분 중 조단백질과 조지방은 각각 P32W86과 P31P41에서 높게 나타났다(P<0.01). NDF와 ADF 함량은 각각 KPO와 KIO에서 높은 경향치를 보였지만 다른 품종과 비교 시 유의적인 차이는 나타내지 않았다. 조섬유 함량은 KIO > KPO > P31P41 > P32W86 > P3156 순으로 높게 나타났다(P<0.01). 총광물질 함량은 KPO가 9,775 ㎎/㎏으로서 다른 품종에 비하여 가장 높게 P3394는 6,651 ㎎/㎏으로서 가장 낮게 나타났다(P<0.01). 총 구성아미노산 함량 및 지방산 함량은 각각 P32K61 및 KIO가 각각 높게 나타났다(P<0.01). 영양수량 중 ha 당 조단백질, 지방산, 구성아미노산 및 TDN 생산량은 각각 P3156이 유의적으로 높게 나타났다(P<0.01). 그러나 조지방 및 광물질 수량은 각각 P31P41 및 KPO가 각각 높게 나타났다(P<0.01). 이상의 결과로 볼 때 사일리지용 옥수수를 논토양에서 재배 시 생육특성, 사초생산량 및 영양수량을 고려하면 P3156, NC+ 7117 및 P31N27 품종이 우수한 것으로 생각된다. This study was carried out to know adaptability and growth characteristics, yield, chemical compositions and nutrition yield of corn hybrids for silage at paddy field. The field experiments were conducted at Sangju province for one year (2009). The experimental design was arranged in a randomized block design with three replication. The treatments consisted of eleven corn hybrids. The planting date was on 1 May and harvested at 24 August. Stem diameter, stem hardness and number of ear were higher in P32P75 than other varieties. Ear height, dead leaf and green degree were highest in NC+7117, but number of root system and Brix (Bº) were higher in P3394 than other varieties. Crude protein and crude fat (EE) were highest in P32K61 and P31P41, respectively (P<0.01). NDF and ADF were highest in KPO and KIO, respectively, but no significant differences were found among the varieties. Total mineral contents were the highest in Kwangpyongok (9,775 ㎎/㎏), and P3394 (6,651 ㎎/㎏) was the lowest as compared to other varieties (P<0.01). Crude protein yield, crude fat yield and mineral yield were highest in P3156, P31P41 and KPO, respectively (P<0.01). Total composition amino acid and total fatty acid were the highest in P32K61 and KIO, respectively (P<0.01). Yields of crude protein, fatty acid, composition amino acid and TDN were the highest in P3156 (P<0.01). But yields of crude protein and mineral were the highest in P31P41 and KPO, respectively (P<0.01). Total digestible nutrient (TDN) was higher in order of P3156 > NC+7117 > P31N27 > KPO > P32K61 > P32T83 > P32P75 > P31P41 > P3394 > P32W86 > KIO. Based on the above results, corn hybrid varieties could be recommended in P3156, NC+7117 and P31N27 for growth characteristics, quantitative production and nutrition yield.

버섯잔사 첨가급여가 육성기 한우 채식행동에 미치는 영향

이상무(Sang Moo Lee),황주환(Joo Hwan Hwang),윤용범(Yong Bum Yoon),곽완섭(Wan Sup Kwak),김영일(Young Il Kim),문상호(Sang Ho Moon),전병태(Byong Tae Jeon) 한국초지조사료학회 2008 한국초지조사료학회지 Vol.28 No.2

본 실험은 NDF 및 ADF 함량이 높은 버섯폐배지(버섯잔사)에 대한 볏짚 대체 가능성을 채식 행동 관점에서 검토할 목적으로 실시하였으며, 시험사료인 농후사료 + 볏짚 (Control : 5.32 ㎏ + 자유채식), 농후사료 + 버섯폐배지 + 볏짚(T1 : 5.32 ㎏ + 0.82 ㎏ + 자유채식) 및 농후사료 + 버섯폐배지 + 볏짚 (T2 : 5.32 ㎏ + 1.64 ㎏ + 자유채식)를 급여하여 채식 행동 조사를 하였다. 공시가축은 평균체중 357.0 ± 2.9 ㎏인 한우 18두를 이용 하였으며, 사료급여에 있어서는 농후사료와 버섯페배지는 1일 2회로 반량씩 급여하였으며 볏짚은 자유채식토록 하였다. 볏짚 채식량은 Control구가 2.66 ㎏으로서 가장 높게, T2구가 2.03 ㎏으로 가장 낮게 나타난 반면, 총채식량 및 NDF 채식량은 T2 > T1 > Control구 순으로 높게 나타났다. 채식시간은 Control구가 289.3분, T2구가 290.5로 비슷했고 T1구가 236.0분으로 가장 적었다 (p<0.05). 반추시간에 있어서는 버섯폐배지 첨가량을 0% (0.0 ㎏), 15% (0.82 ㎏) 및 30% (1.64 ㎏)로 증가 시켜줌에 따라 반추시간은 451.0분에서 359.5분으로 직선적으로 감소하였다 (p<0.05). 이는 버섯폐배지의 사료입자 크기가 매우 작았던 것에 기인된 것으로 생각된다. 1일 저작시간, 식괴수, 저작회수와 식괴당 저작수 및 사료가치지수는 볏짚을 급여한 대조구에서 모두 높게 나타났다(p<0.05). 그러나 식괴 당 반추시간은 T2 (69.4분)에서, 분 당 식괴수는 T1구 (1.07번)에서 높게 나타났다 (p<0.05). 채식율, 반추효율 및 저작효율성에 있어서는 버섯폐배지 첨가 급여구(T1, T2)가 무첨가 급여구 (Control)에 비하여 높게 나타났으며, 이는 높은 채식량과 버섯폐배지의 작은 입자도가 관여한 것으로 생각 된다. 군 행동은 모든 구 (Control, T1 및 T2)에서 농후사료 급여시에 통일된 행동을 보였고, 반추 및 휴식에 있어서 군 행동은 처리 간 큰 차이가 없는 것으로 나타났다. 이상의 결과 육성우 배합사료에 버섯폐배지 15% 및 30%를 첨가 급여함으로서 반추시간, 식괴수, 저작수 및 FVI치는 다소 낮은 결과치를 보였으나, 채식행동에 큰 문제가 될 정도는 아니며, 오히려 채식율, 반추효율 및 저작효율에 있어서 무첨가구에 비하여 첨가구가 높았던 점을 감안 할 때 한우 육성우 조사료원으로서 대체 가능성을 기대할 수 있다. This study was carried out to investigate the effects of dietary supplementation of spent mushroom substrates on eating time, ruminating and resting time of growing Hanwoo. A total of eighteen growing Hanwoo (357.0 ± 2.9 ㎏) were allocated into three feeding groups and assigned to three dietary treatments: Control (C : concentrate + rice straw; 5.32 ㎏ + intake of free), T1 (concentrate + spent mushroom substrates + rice straw; 5.32 ㎏ + 0.82 ㎏ + intake of free) and T2 (concentrate + spent mushroom substrates + rice straw; 5.32 ㎏ + 1.64 ㎏ + intake of free). Intake of rice straw was the highest at C (2.66 ㎏), while T2 (2.03 ㎏) was the lowest. But total intake and NDF intake were highly in order of T2 > T1 > C (p<0.05). Eating time was similar in C and T1 with 289.3 and 290.5 minutes, and that in T1 was the shortest with 236.0 minutes (p<0.05). When spent mushroom substrates were increased by 0 (0.0 ㎏), 15 (0.82 ㎏) and 30% (1.64㎏) of concentrate, chewing time was decreased linearly from 451.0 and 402.3 to 359.5 minutes (p<0.05), which was due to the particle size of very small feed. Number of bolus, number of chews, number of chews/bolus and FVI (chewing time/intake of feed 1㎏) showed the highest to all in C. But ruminating time per bolus was the longest in T2 (69.4 min.), and number of bolus per minute was the highest at T1 (1.07 no.) Eating rate, ruminating efficiency and chewing efficiency were significantly higher (p<0.05) in T1 and T2 than in C, which was due to increased feed intake and the small particle size of spent mushroom substrates. When fed with concentrate, Hanwoo group did united behavior in the all treatment (C, T1 and T2). But group behavior in ruminating and resting was hardly influenced by feeding levels of roughage. Consequently, spent mushroom substrates could be used up to from 15 to 30% in growing Hanwoo roughage source without any harmful effect on eating behavior.