正常血壓白鼠와 單一腎臟 및 腎動脈結紮高血壓白鼠에서의 心筋 Na^+, K^+-ATPase에 關한 硏究

李載欣,沈雲澤 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

Transferrin Receptors in the Liver Cell Membrane of Carcinogen (3-methyl-4-dimethyl-arninoazobenzene) Treated Rat

이재흔,노의선,허강민,이충식,석정호,Lee, Jae-Heun,Ro, Eu-Sun,Hur, Gang-Min,Lee, Choong-Sik,Seok, Jeong-Ho The Korean Society of Pharmacology 1993 대한약리학잡지 Vol.29 No.1

화학물질에 의한 간암 유발과정에서 transferrin receptor (TfR)의 변동을 밝히기 위해 간을 부분절제한 정상백서의 재생간과 발암물질로 3-Me-DAB를 8주간 투여한 백서 또는 약물 투여 후 부분 간절제 수술을 행하여 세포분열을 유도시킨 백서 간조직으로부터 parenchymal cell (PC)과 nonparenchymal cell (NPC)를 분리하고 각각의 세포막을 제조하여 $^{125}I-transferrin$ 결합실험을 실시한 바 다음과 같은 성적을 얻었다. 1. 3-Me-DAB 투여에 의하여 간조직에서 oval cell의 증식, 재생성 변화, 결절형성, 담관의 증식 및 담관세포암 등의 현저한 조직학적 변화가 동반되었다. 그러나 간세포증식을 더욱 촉진시키기 위하여 부분간절제 수술을 하였을 때 수술 후 경과에 따른 형태학적 변동은 큰 차이가 없었다. 2. 정상 재생간의 PC 및 NPC homogenate에서 transferrin 결합량은 부분간 절제 수술 후 1일 및 3일에 증가되었으며 수술 후 7일에 정상으로 회복되었다. 3-Me-DAB 투여에 의해 두세포군에서 모두 정상세포보다 높게 나타났으며 재생기간에 따라 계속 증가되었다. 3. 정상간의 NPC 세포막에서 transferrin 최대 결합량 (Bmax)은 PC 세포막에서 보다 많이 분포되어 있었으며, Kd는 양세포막에서 5.05 또는 6.3nM로 비슷하였다. 4. 재생간의 NPC 및 PC 세포막에서 transferrin 결합량은 부분 간절제 수술 후 1일 및 3일에 $40{\sim}50%$ 증가되었고 수술 후 7일에 정상치로 회복되었다. 5. 3-Me-DAB 처치에 의하여 NPC 및 PC 세포막의 transferrin 결합량은 정상 간세포막에서 보다 약 3배 증가되었고, 3-Me-DAB 투여후 재생간의 NPC 세포막에서는 부분 간절제 수술 후 3일까지 증가된 후 감소되는 양상인데 반해 PC 세포막에서는 수술 후 7일까지 계속 증가되었다. 6. 3-Me-DAB 투여 후 NPC 및 PC 세포막 transferrin binding site에서 Kd치가 $3.1{\sim}4.1\;nM$과 $25.4{\sim}54.1\;nM$인 두 종류가 존재하는 것으로 나타났다. 이상의 실험성적으로 TfR는 1) 간조직의 PC 및 NPC 세포에 모두 분포되어 있으며, 2) 정상 재생간 및 3-Me-DAB의 처리 후 간세포에서의 세포막 TfR의 증가는 세포내 합성량의 증가에 의하여 일어나며, 3) 정상 재생간의 세포막 TfR는 한 종류의 high affinity site $(Kd,\;<3.1{\sim}7.5\;nM)$에 의하여 증가되나, 3-Me-DAB 처리 후 간세포막에서는 정상에서와 같은 high affinity형 이외에 affinty가 낮은 다른 형태의 TfR $(Kd,\;25.4{\sim}54.1\;nM)$가 세포막으로 출현됨으로써 크게 증가되는 것으로 사료된다. To investigate the alteration of transferrin receptor (TfR) in the proliferating or transformed liver cells, $^{125}I-transferrin$ binding experiment was carried out in the isolated parenchymal cells (PC) or nonparenchymal cells (NPC) from normal regenerated rat liver after partial hepatectomy and from the liver of 3-methyl-4-dimethyl-aminoazobenzene (3-Me-DAB) treated rat. With the administration of 3-Me-DAB for 8 weeks, the liver tissue showed marked morphologic changes of oval cell proliferation, regenerations of hepatocytes, and atypical proliferations of bile ducts, but these changes were little affected by partial hepatectomy. Transferrin binding values in PC or NPC homogenate from the regenerated liver of normal rat, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With the treatement of 3-Me-DAB for 8 weeks, transferrin binding sites in homogenates were higher than those of normal rat liver and increased by 7th day after partial hepatectomy. Transferrin binding sites (Bmax) in the cell membrane of NPC were higher than those of PC of normal rat liver, but there was no significant difference in Kd values between both groups (5.05, 6.3 nM). In the normal resenerated rat liver, transferrin binding sites in the PC or NPC plasma membrane, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With 3-Me-DAB tratment, transferrin binding sites in both liver NPC and PC plasma membrane were increased about 3 folds, compared to those in each plasma membrane of normal rat liver. And after partial hepatectomy of 3-Me-DAB trated rat, transferrin binding sites were increased by the 3rd day in the NPC plasma membrane but increased by the 7th day in the PC plasma membrane. In the transferrin binding sites of the PC or NPC plasma membrane of 3-Me-DAB treated liver, two kinds of Kd values $(3.1{\sim}4.7\;nM,\;25.4{\sim}54.1\;nM)$ were detected. The present results suggest that 1) TfRs are distributed in the liver PC as well as NPC; 2) Increased TfRs in PC or NPC plasma membrane of normal regenerated liver after partial hepatectomy and 3-Me-DAB treated rat liver, may be due to increased intracellular synthesis; 3) Increased TfRs in normal regenerated liver after partial hepatectomy might be related to the expression of a single type of high affinity site $(Kd,\;3.1{\sim}7.5\;nM)$, but in 3-Me-DAB treated rat liver might be related to the expression of high and low affinity types of receptors $(Kd,\;25.4{\sim}54.1\;nm)$.

芍藥根이 家兎血壓 및 剔出心房에 미치는 影響

李載欣,李神雄,曺圭朴 충남대학교 의과대학 지역사회의학연구소 1975 충남의대잡지 Vol.2 No.2

Effects of water extract of Paeoniae Radix(PWE) on the blood pressure and inotropic action of isolated atria in the rabbit were investigated. The results were as follows. 1) The doses over 10mg/kg of PWE caused transient fall of the rabbit's arterial blood pressure, which was not affected by bilateral vagotomy, hexamethonium-, propranolol-, or methysergide-pretreatment but inhibited by atropine-pretreatment. 2) PWE, in dose of 3mg and 10mg per ml, induced negative inotropic effect, which was not affected by hexamethonium but markedly diminished or abolished by atropine and potentiated by eserine. The above data suggest that cholinergic mechanism plays an important role in both the hypotensive and negative inotropic effect by PWE in the rabbit.

재생간의 성장과정에 따른 ^3H-ouabain의 결합에 관한 연구

이재흔,허강민 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

To study the changes of Na, K-ATPase in the regenerating liver cell prepared the sinusoidal and canalicular membrane from normal and regenerating rat liver after partial hepatectomy, and Na, K-ATPase activity and ^3H-ouabain binding were measured. 1. In the control liver, Na, K-ATPase activity of sinusoidal membrane was 23.8μmole Pi/mg protein/hr and that of canalicular was 7.0 μmole Pi/mg protein/hr. But the numbers of ^3H-ouabain binding sites were almost same in both membranes. 2. In the regenerated liver for 3 days, the activity of Na, K-ATPase of sinusoidal membrane was decreased about 50% as compared to that of control liver. 5 days after partial hepatectomy, the activity of Na, K-ATPase was restored. 3. Ouabain concentration for the inhibition of Na, K-ATPase activity(IC_50) of sinusoidal membrane in the regenerated and control liver were 5.0 X 10 exp(-5)M and 3.8 X 10 exp(-5)M respectively. 4. In the ouabain binding experiment of sinusoidal membrane, there were little difference in Kd values between both group, but B_max in the regenerated liver was decreased about 50% compared to that of control liver. From the above results, it is suggested that the decreased Na, K-ATPase activity in the sinusoidal membrane during regeneration is due to the decreased numbers of ouabain binding sites, and Na, K-ATPase is distributed in canalicular membrane as well as sinusoidal membrane.

側腦室內 Neostigmine이 家兎 腎臟機能에 미치는 影響

李載欣,李神雄,曺圭朴 충남대학교 의과대학 지역사회의학연구소 1976 충남의대잡지 Vol.3 No.1

Effect of neostigmine administered intracerebroventricularly on the renal function of rabbits was investigated. 1) Intraventricular neostigmine induced decrease after initial increase of urine flow and changes of renal plasma flow, glomerular filtration and urinary sodium and potassium excretion in proportion to urine flow approximately, but did not influence to filtration fraction and reabsorption of sodium and potassium. 2) Correlation coefficient between changes of two parameters among urine volume, renal plasma flow, glomerular filtration and urinary sodium excretion was statistically significant. 3) Increasing changes of various parameters of renal function induced by intraventricular neostigmine were inhibited by intravenous atropine pertreatment and decreasing changes of the parameters partially inhibited by the blocking agent. Pretreatment with intravenous regitine potentiated diuretic action and inhibited antidiuretic action of intraventricular neostigmine. From the above results, it is suggested that the of intraventricular neostigmine on the renal function of rabbits is due to renal hemodynamic changes by central parasympathetic and sympathetic activation.

BASS 확산 모형을 이용한 국내 자동차 외장 램프 LED 수요예측 분석

이재흔 한국품질경영학회 2022 품질경영학회지 Vol.50 No.3

Purpose: Compared to the rapid growth rate of the domestic automotive LED industry so far, the predictive analysis method for demand forecasting or market outlook was insufficient. Accordingly, product characteristics are analyzed through the life trend of LEDs for automotive exterior lamps and the relative strengths of p and q using the Bass model. Also, future demands are predicted. Methods: We used sales data of a leading company in domestic market of automotive LEDs. Considering the autocorrelation error term of this data, parameters m, p, and q were estimated through the modified estimation method of OLS and the NLS(Nonlinear Least Squares) method, and the optimal method was selected by comparing prediction error performance such as RMSE. Future annual demands and cumulative demands were predicted through the growth curve obtained from Bass-NLS model. In addition, various nonlinear growth curve models were applied to the data to compare the Bass-NLS model with potential market demand, and an optimal model was derived. Results: From the analysis, the parameter estimation results by Bass-NLS obtained m=1338.13, p=0.0026, q=0.3003. If the current trend continues, domestic automotive LED market is predicted to reach its maximum peak in 2021 and the maximum demand is $102.23M. Potential market demand was $1338.13M. In the nonlinear growth curve model analysis, the Gompertz model was selected as the optimal model, and the potential market size was $2864.018M. Conclusion: It is expected that the Bass-NLS method will be applied to LED sales data for automotive to find out the characteristics of the relative strength of q/p of products and to be used to predict current demand and future cumulative demand.

고정도구 사용에 의한 경추부 자기공명영상의 움직임 인공물 개선 방안

이재흔,박리사,장재홍,최성규,이정은,김태규,송경철,강남구 대한자기공명기술학회 2014 대한자기공명기술학회지 Vol.24 No.-

유방 자기공명영상검사에서 Reduced-FOV EPI 기법과 Additional presaturation 사용에 의한 인공물 영상의 개선

이재흔,장재홍,박리사,최성규,이정은,김태규,조혜진,김강수,강남구 대한자기공명기술학회 2015 대한자기공명기술학회지 Vol.25 No.-

側腦室內 Physostigmine이 家兎 腎臟機能에 미치는 影響

李載欣 충남대학교 의과대학 지역사회의학연구소 1977 충남의대잡지 Vol.4 No.1

Effect of intraventricular physostigmine on the renal function of the rabbits was investigated. Intraventricular physostigmine(100,㎍) induced increase of urine volume, Ccr and urinary excretion of sodium and potassium. Vagotomy, intravenous or intraventricular atropine inhibited the renal effects of intraventricular physostigmine. From the above results, it is suggested that the natriuretic effect of intraventricular physostigmine is due to the role of central cholinergic mechanism.

자기공명영상에서 SEMAC의 단계별 적용에 따른 자화 감수성 인공물의 영상 평가: 팬텀 연구 중심으로

이재흔,장재홍,김태규,최성규,김지영,강남구 대한자기공명기술학회 2018 대한자기공명기술학회지 Vol.28 No.1