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황성화(Hwang Sung Hwa),함정식(Ham Chong Sik),김건동(Kim Geon Dong),이정익(Lee Jungl-Ik) 한국디자인학회 2010 한국디자인학회 학술발표대회 논문집 Vol.2010 No.10
최근 스마트폰 사용자 증가에 따라 실제환경에 가상의 정보를 혼합하여 실시간으로 보여주는 증강현실 Augmented Reality기술이 화두가 되고있으며, 기술 변화와 기기의 인터페이스 형태에 따라 다양한 유형의 증강현실 컨텐츠가 제작, 발표되고 있다.
성화식(Hwa-Sik Seong),이주민(Ju-Min Lee),황대석(Dae-Seok Hwang),김용덕(Yong-Deok Kim),김욱규(Uk-kyu Kim),김종렬(Jong-Ryoul Kim),정인교(In-Kyo Chung),신상훈(Sang-Hun Shin) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.2
Purpose: The purpose of this study is to investigate the clinical and histological features of odontogenic keratocyst Patients and Methods: A retrosective review of 100 patients who were diagnosed as odontogenic keratocyst by hitological findings during the period of January 2000 and December 2005 in the Dept. of Oral and Maxillofacial surgery Pusan National University was consecuted. For each patient, age, sex, location of lesion, initial diagnosis by radiographic features, treatment procedure, hitologic findings and recurrance rate were evaluated. Results: In this study, OKC has male prevalance to female by 1.38:1, and most likely occurs during third decade. The most common site of lesion was mandibular ramus region(34.6%) and the most common symptom was swelling(50%). The most common initial diagnosis by radiographic findings was OKC and cyst enucleation was the most common treatment method. The recurrance rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. Conclusion: In this study, total recurrence rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. But, since 97% of patients were treated by enucleation and adjuntive excision, further styudy is need about concordance of recurrence rate with surgical method.
UV조사에 의해 유도된 피부섬유아세포의 세포사에 미치는 Sea Buckthorn (Hippophae rhamnoides L.) 열매추출물의 영향
황인식(In Sik Hwang),고은경(Eun Kyoung Koh),김지은(Ji Eun Kim),이영주(Young Ju Lee),곽문화(Moon Hwa Kwak),고준(Jun Go),성지은(Ji Eun Sung),송성화(Sung Hwa Song),황대연(Dae Youn Hwang) 한국생명과학회 2014 생명과학회지 Vol.24 No.5
Sea buckthorn (Hippophae rhamnoides L.)은 flavonoid, carotenoids, steroids, vitamin, tannins, oleic acid 등의 생물학적으로 활성이 높은 성분을 풍부하게 함유하고 있어 오랫동안 피부질환(skin diseases), 위궤양(gastric ulcers), 천식(asthma), 폐질환 등에 사용되어 왔다. 본 연구에서는 아시아와 유럽의 고지대에서 야생으로 자라는 Sea buckthorn의 열매추출물(SBFE)이 UV조사 후 유도되는 세포사와 연관성을 규명하기 위하여, 세포사와 관련된 다양한 인자의 변화를 관찰하였다. 그 결과, 세포사를 유발할 수 있는 UV조사농도는 400 mJ로 설정하였고, 세포생존율은 UV + LoC, UV + MeC, UV + HiC 처리군에서는 유의적으로 증가하였다. 또한 FACS와 DAPI염색의 결과에서 PI로 염색된 세포수는 UV + vehicle 처리군에서 급격히 증가한 이후 UV + SBFE 처리군에서 유의적으로 감소하였고, Annexin V가 염색된 세포 수는 고농도의 SFBE을 처리한 UV + HiC 처리군에서 유의적으로 감소하였다. 그러나 PI와 Annexin V가 동시에 염색된 세포의 수는 UV + LoC 처리군에서 오히려 증가하였고 나머지 그룹에서는 유의적인 변화가 관찰되지 않았다. 한편, 세포사와 관련된 단백질의 변화에 대한 연구에서 caspase-3는 pro 형태와 active 형태 모두에서 유의적인 차이를 나타내지 않았으며, Bax와 Bcl-2 모두 UV 무처리군에 비하여 UV + Vehicle 처리군에서 감소하였으나 Bax의 발현양은 UV + SBFE 처리군에서 농도의존적으로 증가한 반면, Bcl-2의 발현양은 UV + SBFE 처리군에서 추가적으로 더욱 감소하였다. 따라서 이상의 결과들은 SBFE가 UV조사에 의해 유도된 세포사를 억제하거나 회복시키는 기능을 가지고 있음을 제시하고 있어 향후 UV조사에 의해 유발되는 다양한 상해에 대한 치료제로서의 가능성을 제시하고 있다. Sea buckthorn (Hippophae rhamnoides L.) is a well-known and rich source of biologically active compounds, such as flavonoids, carotenoids, steroids, vitamins, tannins, and oleic acid. The effects of sea buckthorn fruit extract (SBFE) on ultraviolet (UV)-induced cell death was investigated in SK-MEL-2 cells cotreated with UV and a low concentration (LoC), medium concentration (MeC), or high concentration (HiC) of SBFE. Cell viability gradually decreased in accordance with an increase in the UV dose. The cell viability of the UV+SBFE cotreated cells increased significantly compared to that of UV+vehicle-treated cells during the application of an appropriate UV radiation dose (400 mJ). In addition, the number of 4’,6-diamidino-2-phenylindole (DAPI), propidium iodine (PI)-, and annexin V-stained apoptotic cells was higher in the UV+vehicle-treated cells than in the UV untreated cells. The decrease of apoptotic cell numbers varied in each treated group, but it was most significant in the SBFE-treated group. The number of PI-stained cells dramatically decreased in accordance with the concentration of SBFE, and the maximum decrease was detected in the UV+HiC-treated group. In addition, Bax expression increased and Bcl-2 expression decreased in the SBFE-treated group compared with the UV-only treated group. The level of caspase-3 remained constant in all the groups. These results suggest that SBFE may contribute to a recovery from UV-induced cell death through the regulation of apoptotic protein expression and that it may have potential therapeutic utility in ameliorating UV-induced skin ageing.
전자선 조사한 아몬드(Prunus amygosalus L.)와 땅콩(Arachis hypogaea)에서 유래한 지방분해산물 분석
정인선(In Seon Jeong),김재성(Jae Sung Kim),황인민(In Min Hwang),최성화(Sung Hwa Choi),최지연(Ji Yeon Choi),노은영(Eun Yeong Nho),나임칸(Naeem Khan),김병숙(Byung Sook Kim),김경수(Kyong Su Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.1
아몬드와 땅콩의 비조사 시료와 1, 3, 5, 10 kGy로 전자선을 조사한 시료에서 유도된 hydrocarbon류의 생성량을 분석하기 위하여 지방을 n-hexane으로 추출하여 florisil column으로 분리 및 GC/MS로 분석하였다. 전자선 조사된 아몬드와 땅콩에서 유래한 hydrocarbon류는 oleic acid에서 1,7-hexadecadiene(C<SUB>16:2</SUB>)과 8-heptadecene(C<SUB>17:1</SUB>), linoleic acid에서 1,7,10-hexadecatriene(C<SUB>16:3</SUB>)과 6,9-heptadecadiene(C<SUB>17:2</SUB>), 그리고 palmitic acid에서 1-tetradecene(C<SUB>14:1</SUB>) 및 pentadecane(C<SUB>15:0</SUB>) 생성되었고, oleic acid와 palmitic acid에서는 C<SUB>n-2</SUB>, linoleic acid에서는 C<SUB>n-1</SUB> 화합물이 보다 더 높게 생성되었다. 전자선을 1, 3, 5, 10 kGy의 선량별로 조사한 시료에서 생성된 hydrocarbon류의 함량은 조사선량에 따라 증가하였고 비조사 시료에서는 확인되지 않았다. 특히, oleic acid와 linoleic acid에서 유래한 1,7-hexadecadiene(C16:2), 8-heptadecene(C17:1)과 1,7,10-hexadecatriene(C<SUB>16:3</SUB>) 및 6,9-heptadecadiene(C<SUB>17:2</SUB>)의 경우 아몬드와 땅콩 모두에서 다른 hydrocarbon류에 비해 전자선 조사에 의한 생성량이 높게 나타난바 전자선 조사유무 판별 marker로 사용 가능함을 확인하였다. Food irradiation has recently become one of the most successful techniques to preserve food with increased shelf life. This study aims to analyze hydrocarbons in almonds (Prunus amygosalus L.) and peanuts (Arachis hypogaea) induced by electron beam irradiation. The samples were irradiated at 0, 1, 3, 5 and 10 kGy by e-beam and using florisil column chromatography fat, and content was extracted. The induced hydrocarbons were identified using gas chromatography-mass spectrometry (GC/MS). The major hydrocarbons in both irradiated samples were 1,7-hexadecadiene (C<SUB>16:2</SUB>) and 8-heptadecene (C<SUB>17:1</SUB>) from oleic acid, 1,7,10-hexadecatriene (C<SUB>16:3</SUB>) and ,9-heptadecadiene (C<SUB>17:2</SUB>) from linoleic acid and 1-tetradecene (C<SUB>14:1</SUB>) and pentadecane (C<SUB>15:0</SUB>) from palmitic acid. Concentrations of the hydrocarbons produced by e-beam were found to be depended upon the composition of fatty acid in both almonds and peanuts. The Cn-2 compound was found to be higher than Cn-1 compound in oleic acid and palmitic acid, while in case of linoleic acid, Cn-1 compound was higher than Cn-2 compound. The radiation induced hydrocarbons were detected only in irradiated samples, with 1 kGy or above, and not in the non-irradiated ones. The production of 1,7-hexadecadiene (C<SUB>16:2</SUB>), 8-heptadecene (C<SUB>17:1</SUB>), 1,7,10-hexadecatriene (C<SUB>16:3</SUB>) and 6,9-heptadecadiene (C<SUB>17:2</SUB>), in high concentration gave enough information to suggest that these may be the possible marker compounds of electron beam irradiation in almonds and peanuts.
PVD법에 의해 제작한 Al-Mg 코팅 강판의 내식성에 미치는 Mg 함량 및 열처리의 영향
강재욱(Jae Wook Kang),박준무(Jun-Mu Park),황성화(Sung-Hwa Hwang),이승효(Seung-Hyo Lee),문경만(Kyung-Man Moon),이명훈(Myeong-Hoon Lee) 한국표면공학회 2016 한국표면공학회지 Vol.49 No.2
This study was intended to investigate the effect of the amount of magnesium addition and heat treatment in the Al-Mg coating film in order to improve corrosion resistance of aluminum coating. Al-Mg alloy films were deposited on cold rolled steel by physical vapor deposition sputtering method. Heat treatment was fulfilled in an nitrogen atmosphere at the temperature of 400°C for 10 min. The morphology was observed by SEM, component and phase of the deposited films were investigated by using GDLS and XRD, respectively. The corrosion behaviors of Al-Mg films were estimated by exposing salt spray test at 5 wt.% NaCl solution and measuring polarization curves in deaerated 3 wt.% NaCl solution. With the increase of magnesium content, the morphology of the deposited Al-Mg films changed from columnar to featureless structure and particle size was became fine. The x-ray diffraction data for deposited Al-Mg films showed only pure Al peaks. However, Al-Mg alloy peaks such as Al₃Mg₂ and Al12Mg17 were formed after heat treatment. All the sputtered Al-Mg films obviously showed good corrosion resistance compared with aluminum and zinc films. And corrosion resistance of Al-Mg film was increased after heat treatment.