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Ootimization of Mekium Components for Lactic Acid Production
조윤경,조규홍,홍승서,이현수,Cho, Yun-Kyung,Cho, Kyu-Hong,Hong, Seung-Suh,Lee, Hyun-Soo 한국미생물 · 생명공학회 1995 한국미생물·생명공학회지 Vol.23 No.1
Medium components for lactic acid production were optimized with a strain of Lactobacillus sp., isolated by our Lab. Nitrogen source was the key component and manganese ion was also important for lactic acid production in this strain. Optimal concentration of manganese ion was 0.03 g/l as MnSO$_{4}$ 4 - 5 H$_{2}$O base. Other mineral elements, however, had little effect on it. Among the nitrogen sources we examined, yeast extract showed the highest productivity. Yeast extract, the exellent but very expensive medium component, could be partially replaced by soytone until 60% dry base with higher productivity, or by tryptone enforced with vitamines and nucleic acids. In order to replace yeast extract completely, we examined several inexpensive nitrogen sources and their enzymatic hydrolyzates. The hydrolyzate of vital wheat gluten was the best of them.
식물세포배양에 의한 항암제 Paclitaxel의 대량 생산
최형균,손주선,나광휘,홍승서,박연성,송재영,Choi, Hyung-Kyoon,Son, Joo-Sun,Na, Gwang-Hwee,Hong, Seung-Suh,Park, Yeon-Seung,Song, Jai-Young 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1
Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The paclitaxel-Genexol$^{TM}$-is commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.