Flavobacterium hercynium EPB-C313 균주를 이용한 배추 뿌리혹병 생물적 방제

함수상,김종태,한광섭,김병련,김홍규,남윤규,유승헌 한국식물병리학회 2012 식물병연구 Vol.18 No.3

Clubroot of Chinese cabbage by Plasmodiophora brassicae, was found to be high virulent to the Chinese cabbage, turnips and cabbage. It this study, the endophytic bacteria Flavobacterium hercynium EPB-C313,which was isolated from tissues of Chinese cabbage, was investigated the antimicrobial activity on the inactivation of resting spores and its control effect on clubroot disease by bioassay. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivate the resting spores of P. brassicae with 90.4, 36.8, and 26.0%, respectively. The clubroot was inhibited with 100% by dipping the seedlings of Chinese cabbage in culture solutions of F. hercynium EPB-C313 before planting, however the chemmical ‘fluazinam’was 91.7% in pot tests. Complex treatment were highly enhanced control efficacy with 63.7% at field of 50%diseased plants by soil incorporation with the pellet contains organic matter and F. hercynium EPB-C313,seedling drench of culture solution of F. hercynium EPB-C313 and soil drench with 10 days after planting. These results imply that the F. hercynium EPB-C313 is a very useful biological control agent of clubroot disease of Chinese cabbage. 0

재배 형태별 백합 잎마름병의 발생 양상 및 몇가지 경종적 처리의 방제 효과

함수상,이종원,유승헌,이기환,이희덕 한국식물병리학회 2007 식물병연구 Vol.13 No.3

Incidences of leaf blight of lily cultivars Raizan and Casa Blanca in the open field cultivation were 50% and 45.4%, respectively, while those in the green house cultivation were significantly reduced to 1.5% and 1.9%, respectively. In the green house, the incidences of the disease in sprinkler watering cultivation were 14.5~16.5%, while those in drip watering cultivation were only 1.5~2%. Incidence of the disease was severe in the field where the lily was cultivated successively for 2 to 3 years. Isolation frequencies of B. elliptica from overwintered plant debrises such as leaves, stems, capsules, and bulbs were 43.3%, 46.7%, 60% and 0%, respectively, while those of B. cinerea were 10.3%, 0%, 3.3% and 0%, respectively. Incidence of leaf blight in the field where diseased plant debris was cleaned was 7.3%, while that in the field where diseased plant debris was not cleaned was 56.5%. Incidences of the disease in the field where coverages of soil surface with black vinyl, bark or rice straw were used were 6.6%, 8.2% and 11.3%, respectively, while that in the field where the coverage was not used was 21.3%.

나리에서 분리한 잎마름병균의 살균제에 대한 감수성 변화와 포장 방제

함수상,경기천,김병련,한광섭,최종진,남운규,유승헌 한국식물병리학회 2013 식물병연구 Vol.19 No.1

국내 나리 주산단지에서 분리한 Botrytis elliptica 48개균주와 Botrytis cinerea 23개 균주를 대상으로 농가포장에서 사용되고 있는 살균제에 대한 약제저항성 검정을 실시하였다. 살균제 benomyl과 mancozeb에 대한 EC50 값은500−1,000 μg/ml로 시험에 사용된 모든 약제 중에서 가장 높게 나타나 이 약제들은 나리재배 포장에서 잎마름병에 대한 방제 효과가 거의 없을 것으로 판단되었다. 또한 나리 재배농가에서 잎마름병 방제약제로 가장 많이 사용되고 있는 dicarboximide계 procymidone 살균제와iprodione 살균제의 경우, EC50 값 5−50 μg/ml은 각각93.7%와 100%이었고, diethofencarb/carbendazim 합제와fludioxonil 살균제의 EC50 값 0−0.1 μg/ml 범위는 각각98.0%와 93.8%이었다. 한편, 비가림 포장 재배에서의 잎마름병 방제 효과는 발병 직후 iprodione 약제, diethofencarb/carbendazim 합제, fludioxonil 약제를 교호적으로 4회 살포하였을 때 가장 우수하였다. Forty eight isolates of Botrytis elliptica and 23 isolates of B. cinerea from several locations in Korea were tested for resistance to fungicides used in the farmer's fields. Isolation frequency of B. elliptica having EC50 (effective concentration of 50%) value 500−1000 μg/ml to benomyl and mancozeb appeared highly, suggesting that the two fungicides are not effective in controlling leaf blight of lily in the field. The isolates were tested for resistance to fungicides procymidone and iprodione which were most commonly used in the farmer's fields. The rates of EC50 value 5−50 μg/ml to procymidome and iprodione were 93.7% and 100%, respectively, and those of 0−0.1 μg/ml to diethofencarb+carbendazim and fludioxonil were 98.0% and 93.8%, respectively. In the rain-protected cultivation, control of leaf blight of lily was the most effective when iprodine,diethofencarb+ carbendazim, and fludioxonil were sprayed alternately four times during the growing season.

백합 저장중 구근부패에 관여하는 병원균의 동정과 종구 소독효과

함수상,유승헌,오서용,이은모 한국식물병리학회 2006 식물병연구 Vol.12 No.1

Several pathogens associated with bulb rot of lilies in storage house were identified with respect to rot types. Rot patterns were grouped into four different types; brown rot of bottoms, brown rot of shoots, water-soaked rot and blue mold. Brown rot of bottoms was the highest in frequency with 72.5%, and brown rot of shoots the least with 23.0%. Dominant pathogens were differed with rot patterns, brown rot of bottoms by Fusarium oxysporum, blue mold and brown rot of shoots by Penicillium brevicompactum and P. fellutanum. In woundinoculation tests, Penicillium and Fusarium isolates caused severe rot on the bulbs. Bulb disinfection before storage by

Rhizopus oryzae에 의한 백합 무름병

함수상,홍계완,김병련,한광섭,최택용,남윤규,유승헌 한국식물병리학회 2014 식물병연구 Vol.20 No.1

Rhizopus soft rot of lily (Lilium longiflorum) caused by Rhizopus oryzae was observed in the experimental fieldin Taean Lily Experiment Station in Korea, 2012. The typical symptoms were water-soaked lesions on bottomstem and leaf rot. The lesion rapidly expanded and the plant was softened totally. The fungus grew vigorouslyat an optimum temperature (25oC) and brownish colony and black sporangia were formed on potatodextrose agar medium. Sporangiophores formed on end of sporangia were sub-globose, brownish and 6-10μm in size. Sporangia were globose, blackish and 87-116 μm in size. Sporangiospores were irregularly ovaland sub-globose, brownish 4-8 μm in size. On the basis of mycological characteristics, analyzing sequences ofinternal transcribed spacer region of ribosomal DNA, and pathogenicity test on host plants, the causal funguswas identified as R. oryzae. This is the first report of Rhizopus soft rot on lily caused by R. oryzae in Korea.

Alternaria mali에 의한 아로니아 점무늬낙엽병

함수상,권미경,김병련,한광섭,남윤규 한국식물병리학회 2016 식물병연구 Vol.22 No.1

In early June 2014, leaf spot symptoms were observed on black chokeberry (Aronia melanocarpa) inYesan-gun and Geumsan-gun, Chungcheongnam-do in Korea. The initial symptoms on leave surfaceswere brown small-circular spots with a yellow halo lesion, and gradually the small spots were fused,all of infected leaves dropped eventually. A fungus were isolated from the initial lesion, and culturedon potato dextrose agar. Colony color on upper surface of plate varied from olive gray to charcoalgray. Size of conidia mostly extend to 19–50×5–9 μm in nature and 20–59×8–13 μm in culture, with3–8 transverse septa and usually no longitudinal septum or only 1 longitudinal septum in 1–3 ofthe transverse compartments, and also have a short or long beak. Pathogenicity was investigatedusing wounded or unwounded black chokeberry and apple leaves. After 7 days of inoculation,leaf spots were similar to the symptoms naturally occurred in the field. On the basis of mycologicalcharacteristics, pathogenicity, and ITS rDNA sequence analysis, this fungus was identified as Alternariamali. This is the first report of Alternaria leaf spot on black chokeberry caused by A. mali in Korea.

Sclerotinia sclerotiorum에 의한 바질 균핵병

함수상,김병련,한광섭,권미경,박인희,Hahm, Soo Sang,Kim, Byoung Ryun,Han, Kwang Seop,Kwon, Mi Kyung,Park, In Hee 한국식물병리학회 2017 식물병연구 Vol.23 No.1

2011년부터 2013년까지 예산 지역의 농가포장에서 재배 중인 바질에서 균핵병으로 의심되는 증상이 지속적으로 관찰되었다. 초기에는 잎과 줄기가 갈색으로 변하기 시작하여 반점이 커지면서 점차 위쪽으로 진전, 위조하여 결국 전체적으로 썩어 죽는다. 잎과 줄기의 병반부에는 다량의 흰색 균사가 발생하며 $30-100{\mu}m$ 직경의 균핵이 형성하였다. PDA상에 균체는 흰색과 옅은 초콜릿 흑색으로 다양한 색을 나타내었고, 균핵은 검은색으로 대부분 불규칙한 구형이며 크기는 $5-50{\mu}m$였다. 병원균을 인위적으로 접종한 바질의 잎과 줄기에서는 자연 발생된 바질과 동일한 괴사와 위조증상을 보이며, 병반에서 역시 같은 균을 분리하였다. 이상과 같이 병원균의 균학적 특징, ITS 염기서열 분석, 병원성 검정 결과 본 병해는 Sclerotinia sclerotiorum에 의한 바질균핵병으로 명명할 것을 제안한다. During growing season of 2011 to 2013, Sclerotinia rot symptoms consistently have been observed on basil in Yesan-gun, Chungcheongnam-do in Korea. The typical symptom formed initially brownish spot on leaf and stem, and then advancing margins, wilting the whole plant and blighting, eventually died. On the surface of diseased lesions was observed cottony, white, dense mat of mycelial growth, and sclerotia ($30-100{\mu}m$ diameter) formed on stem and leaf. Morphological and cultural characteristic on potato dextrose agar, color of colony was white and colorless chocolate, sclerotium of irregular shape of the oval was black and $5-50{\mu}m$ diameter in size. In pathogenicity test, necrosis and wilt of the inoculated stem were observed in all plants and the pathogen was reisolated from stems. On the basis of mycological characteristics, pathogenicity, and internal transcribed spacer rDNA sequence analysis, this fungus was identified as Sclerotinia sclerotiorum. This is the first report of Sclerotinia rot on basil caused by S. sclerotiorum in Korea.

고추 탄저병 방제제 Burkholderia lata CAB13001 선발 및 포장방제 효과

함수상,김병련,권미경,한광섭,박인희,서경원 한국유기농업학회 2018 韓國有機農業學會誌 Vol.26 No.4

To control the pepper anthracnose caused by Colletotrichum acutatum, antifungal bacterium strains which was selected among bacterium from natural soil, was tested the antimicrobial activity against various pathogens and its control efficacy on anthracnose disease in the fields. We confirmed that antagonistic activity of CAB13001 strain to pathogens such as Sclerotinia cepivorum, Sclerotinia sclerotium and Botrytis cinerea including Colletotrichum acutatum was remarkable superior with the dual culture method in the artificial medium. In vitro bioassay using the green pepper fruit, CAB13001 strain suppressed the lesion development of Anthracnose disease, and its control value compared to the untreated one was 82.4% on pepper fruit in field test. These results suggested that CAB13001 strain could be a very useful biological control agents to anthracnose disease caused by air born plant pathogens of pepper. By the way, analysis of nucleotide sequence of the gene 16S rDNA, antagonistic bacterium CAB13001 strain used in this study was identified as Burkholderia lata.

나리(백합) 잎마름병에 대한 주요 품종별 저항성 정도 비교

이혜경,한경숙,함수상,유승헌 한국육종학회 2009 한국육종학회 학술발표회 발표요지 Vol.41 No.1

유기농 답전윤환지 Fusarium thapsinum에 의한 수수 줄기썩음병

김병련,한광섭,함수상,강영식,박인희,윤성탁 한국식물병리학회 2016 식물병연구 Vol.22 No.4

In late June 2016, stalk rot symptoms were observed on five vatieties of sorghum (Sorghum bicolar) at organic paddy-upland rotation system in Anseong city, Korea. The initial symptom on stalk surfaces was red color with a dark red spot lesion. A fungus was isolated from the initial lesion, and cultured on potato dextrose agar. Size of microconidia mostly extend to 5–19×2–4 μm in culture, with 0–1 septa and macroconidia extend to 29–52×3–4 μm with 4–6 septa. Pathogenicity was investigated using conidial suspension spray to seedling of sorghum. After 3 days of inoculation, the dark red lesion was produced on stalks. On the basis of mycological characteristics, pathogenicity, and internal transcribed spacer (ITS) rDNA sequence analysis, this fungus was identified as Fusarium thapsinum. This is the first report of stalk rot on sorghum caused by F. thapsinum in Korea.