열처리가 보리 종자에서 추출한 Amylase 활성에 미치는 영향

최재은,전방욱,권영명,Choi, Jae-Eun,Jun, Bang-Ook,Kwon, Young-Myung 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.2

보리(Hordeum vulgare L. cv. Baecdong) 종자에서 추출한 $\alpha$-amylase와 $\beta$-amylase의 활성에 미치는 열처리($70^{\circ}C$)의 영향을 조사하였다. 20분 열처리 후 $\beta$-amylase 활성은 완전히 소실되었으며 대부분의 $\alpha$-amylase 활성 역시 소실되었으나 남아 있는 $\alpha$-amylase의 활성을 측정할 수는 있었다. amylase를 전기영동한 결과 20분간 열처리 후 $\alpha$-amylase band는 남아 있었으나 $\beta$-amylase band는 완전히 소설되었다. 이 결과로 미루어 보아 열처리에 의한 $\beta$-amylase의 선택적 불활성화 방법을 $\alpha$-amylase 활성 측청시에나 $\alpha$-amylase 단백질 정제시에 사용할 때는 충분한 검토가 필요함을 알 수 있다. The effect of heat treatment ($70^{\circ}C$) on the activities of $\alpha$-amylase and $\beta$-amylase extracted from barley (Hordeum vulgare L.cv. Baecdong) seeds was investigated. After 20 min-heat treatment, $\beta$-amylase activity was completely lost and most of $\alpha$-amylase activity was lost, but somewhat detectable $\alpha$-amylase activity remained. Electrophoretic study of amylases revealed that $\alpha$-amylase bands remained but $\beta$-amylase bands were completely lost after 20 min-heat treatment. These results suggest that precautions must be taken when selective $\beta$-amylase inactivation by heat treatment is to be employed to assay $\alpha$-amylase activity or to purify $\alpha$-amylase protein.

무가지 신문에 실린 피부과적 건강 정보의 현황과 신뢰도에 관한 연구

최재은 ( Jae Eun Choi ),안효현 ( Hyo Hyun Ahn ),계영철 ( Young Chul Kye ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.11

Background: There has been increasing concern about dermatological health information among the general population. Despite the substantial amount of dermatological articles in the mass media, little is known about their credibility. Objective: The purpose of this study was to evaluate the credibility of dermatological articles in free newspapers, one of the recently popular mass media. Methods: Articles including dermatological information were selected from 5 free newspapers from July 2006 to February 2007. They were classified according to theme and type of consultant and were evaluated for their credibility. Results: A total of 361 articles were identified. Articles about self skin care (26.6%) were most frequent, followed by cosmetic therapy (18.7%), acne (17.1%), hair (16.5%) and atopic dermatitis (6.3%). As the consultants, dermatologists (38.0%) were most common, followed by oriental medical doctors (21.5%), non-medical consultants doctors (13.3%) and other medical specialists (13.0%). There were also articles without consultant input (14.6%). Credibility assessment revealed that 213 (67.4%) articles were credible, but 91 (28.8%) were incredible and 12 (3.8%) could not be evaluated based on the dermatological references. While most of the information from dermatologists and other medical specialists were credible, those from the other types of consultants or without consultant input showed a much higher proportion of incredible information. Conclusion: This study revealed that there are many incredible dermatological articles in free newspapers, most of which are consulted from those other than dermatologists or other medical specialists. Therefore we suggest that dermatologists need to increase their public health role in the mass media. (Korean J Dermatol 2007;45(11):1132∼1138)

인간 면역결핍 바이러스 감염 환자에서 발생한 Acral Persistent Papular Mucinosis

최재은 ( Jae Eun Choi ),김지웅 ( Ji Woong Kim ),조현민 ( Hyun Min Cho ),안효현 ( Hyo Hyun Ahn ),계영철 ( Young Chul Kye ),김수남 ( Soo Nam Kim ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.9

Acral persistent papular mucinosis (APPM) is a rare subtype of localized lichen myxedematosus, which involves only the extensor surfaces of the hands, wrists and the distal part of forearms. Some subtypes of lichen myxedematosus including APPM have been reported with increased frequency in a patient with human immunodeficiency virus (HIV) infection. We describe a 31 year-old male patient with an HIV infection who presented with discrete, flesh-colored papules on the extensor surfaces of the wrists and the distal part of the forearms. Histopathologic findings revealed focal deposition of mucin in papillary and mid dermis that spared a small grenz zone. The lesions showed spontaneous regression after starting highly active antiretroviral therapy (HAART). We report a rare case of APPM in a patient with HIV infection which showed spontaneous regression with HAART, suggesting that APPM may represent one of the cutaneous markers of HIV infection. (Korean J Dermatol 2007;45(9):951∼955)

신속 영구 절편법을 이용한 모즈 미세도식 수술로 치료한 재발성 피부섬유육종

최재은 ( Jae Eun Choi ),전지현 ( Ji Hyun Jeon ),서수홍 ( Soo Hong Seo ),손상욱 ( Sang Wook Son ),김일환 ( Il Hwan Kim ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.2

Dermatofibrosarcoma protuberans is a dermal spindle cell neoplasm of intermediate malignancy, and is characterized by a high local recurrence rate after surgical excision due to its aggressive, finger-like outgrowth pattern. Mohs micrographic surgery, which ensures complete excision of the tumor and minimizes normal tissue loss, is accepted as the treatment of choice for dermatofibrosarcoma protuberans. However, the use of frozen sections for margin control in dermatofibrosarcoma protuberans is controversial because it could be difficult to differentiate minimal residual tumor from normal skin on the frozen sections, and is more challenging in recurrent disease because of the scar tissue. We report a case of a recurrent dermatofibrosarcoma protuberans in which the tumor was excised using the Mohs surgical technique and margin control was achieved with horizontally-cut paraffin-embedded permanent sections, named as rush permanent sections. (Korean J Dermatol 2007;45(2):175~179)

동,서양의 심리치료 : 교사의 주관적 삶의 질과 영향 요인

최재은 ( Jae Eun Choi ),배정우 ( Jeong Woo Bae ),김정남 ( Jeong Nam Kim ) 한국동서정신과학회 2004 동서정신과학 Vol.7 No.2

피부염의 원인균인 황색포도상구균 내성 균주(MRSA)에 대한 에센셜 오일의 항균 효과

최재은 ( Jae-eun Choi ),장태수 ( Tae-su Jang ),김재경 ( Jae-kyung Kim ) 한국미용학회 2022 한국미용학회지 Vol.28 No.5

The emergence of multidrug-resistant microorganisms represents a global challenge owing to the lack of new effective antimicrobial agents. Essential oils could be an alternative to antibiotics because of their anti-inflammatory, antiviral, antibacterial, and antibiofilm activities. Therefore, this study aimed to investigate the antibacterial activity of essential oils as an alternative to antibiotics. In the present study, we cultured methicillin-resistant Staphylococcus aureus (MRSA) with two concentrations (0.5% and 1.0%) of 17 types of essential oils and the antibiotic oxacillin as the control. The optical density of the culture suspensions at 600 nm was determined to assess the effect of the essential oils on MRSA. At 0.5% concentration, 8 essential oils demonstrated higher antibacterial activity than oxacillin, with palmarosa, melissa true, and rose geranium showing the highest antibacterial activity. At 1% concentration, 13 essential oils showed higher antibacterial activity than oxacillin, with melissa true, palmarosa, lemongrass, and rosemary verbenone showing the highest antibacterial activity. Furthermore, essential oils isolated from leaves showed higher antibacterial ability. Overall, the antibacterial activity of essential oils against MRSA varied with the concentration, type, and extraction site. Therefore, our study provides a basis for the selection and use of essential oils for antibacterial activity against MRSA.

칸디다 알비칸스에 대한 19가지 에센셜 오일과 플루코나졸의 항진균 활성 평가

최재은 ( Jae-eun Choi ),장태수 ( Tae-su Jang ),김재경 ( Jae-kyung Kim ) 한국미용학회 2022 한국미용학회지 Vol.28 No.5

Although many plants have been investigated for their antifungal activity against candidiasis, more treatment options remain needed. This study evaluated the antifungal activity of 19 essential oils against Candida albicans. Nineteen essential oils, at concentrations of 1% and 0.5%, were added to cultures of C. albicans and incubated at 37℃ for 18 h. The optical density was measured at 600 nm. The antifungal activity of the essential oils against C. albicans was dependent on concentration and the extraction site. At 0.5%, essential oils from thyme, palmarosa, and lemongrass displayed higher antifungal activities than fluconazole, suggesting that they are effective against C. albicans. The essential oils of eucalyptus, rosewood, and lavender showed the highest activity at 1% concentration and may be used as antibiotics individually. In addition, the essential oils extracted from leaves, such as thyme, were found to have higher antifungal activity than those from other sites.

범죄로부터 안전한 주거환경 조성을 위한 법ㆍ제도 개선방안 연구

최재은(Choi Jae-Eun),정윤남(Jeong Yun-Nam),김세용(Kim Sei-Yong) 대한건축학회 2011 대한건축학회논문집 Vol.27 No.3

Safety of residents could be considered and then increased when we plan, design, and operate a city. In Korea, CPTED (Crime Prevention through Environmental Design) is currently being applied to new towns. However, it is not systemized and neither efficient, for there are no constraint provisions, and the foreign cases are not specifically customized to the current situation of the country. This means, the introduction of CPTED is an indispensable fact, but there are limits to the budget aids and actual application, for there is no legal base to support it. This study, therefore, aims to find the limits from analyzing related laws and regimes, administrative regulations, and applied cases, and to deduct improvement plans based on examined foreign cases. In this Study, the supporting system of foreign CPTED related laws and regulations was analyzed around the cases of England and U.S.A, and based on that information, the present condition and limits of CPTED related laws and regulations of Korea were deducted. As a result of this study, there were no constraint provisions to apply the design guidelines to actual planning, and there were limits on backup aid and actual application due to the lacking of analyzing the relevant area. Also, an acceptable framework must be arranged by the revision of laws and ordinances to compel the system, and link it with systematized guidelines and private-public cooperation. Furthermore, CPTED certification system and training system for professionals are needed so that revitalize the whole system.

불성실공시지정의 효과

최재은 ( Jae Eun Choi ),최정운 ( Jeong Un Choi ),배길수 ( Gil S Bae ) 한국회계학회 2013 會計學硏究 Vol.38 No.3

불성실공시지정 제도는 상장법인이 자본시장법 및 유가증권시장 공시규정에 의한 공시의무를 성실히 이행하지 않은 경우 이에 대해 거래소가 부과하는 제재조치이다. 불성실공시지정과 관련한 선행연구는 다수 존재하지만 아직까지 불성실공시지정이 대상기업의 행태의 변화에 영향을 미치는지를 조사한 연구는 없다. 불성실공시지정 이후 재무정보의 품질이 향상되는지를 조사하는 것은 규제의 효과를 조사하는 것으로 볼 수 있으며, 따라서 불성실공시지정 규제의 효과를 판단할 수 있는 근거가 된다. 구체적으로 본 연구에서는 (1) 불성실공시에 대한 시장의 반응을 조사하고; (2) 불성실공시 제재를 당한 기업의 재무보고의 품질이 제재를 당하지 않은 기업과 차이가 존재하는 지를 조사하고; 그리고 마지막으로 (3) 불성실공시지정이후 재무보고의 품질이 향상되는지를 미루어 불성실공시로 인한 제재의 효과가 존재하는지를 조사한다. 실증분석 결과는 다음과 같다: (1) 불성실공시와 관련된 두 가지 사건일(지정예고일, 지정일)에 대해 시장은 대체로 유의한 음의 주가반응을 보이고 있다; (2) 불성실공시 지정기업은 비지정기업에 비해서 재무보고의 품질이 낮다; (3) 불성실공시 지정 후 재무보고의 품질이 유의하게 높아진다. 본 연구의 결과는 불성실공시제도가 기업의 행태에 영향을 미쳐 재무보고의 품질을 향상한다는 것으로 해석할 수 있다. “Unfaithful disclosure designation” is a penalty imposed to firms for unfaithful disclosure. Previous studies have examined several issues concerning unfaithful disclosure designation. For instance, Lee et al. (2008) find that unfaithful disclosure designation is associated with lower credit rating, and as a consequence increases firms` cost of capital. Park et al. (2008) demonstrate that the earnings quality of the firms with an unfaithful disclosure designation is lower than that of the control firms. Similarly, Kim et al. (2010) examine the relationship between unfaithful disclosure designation and discretionary accruals. However, no study so far has examined as to how unfaithful disclosure designations alter the firm`s behavior. In particular, it has not been shown whether the designation improves the financial reporting quality of the designated firm in the post-unfaithful disclosure designation period. To fill this void, we directly examine whether the financial reporting quality changes after a designation for the designated firms for unfaithful disclosure. Specifically, we investigate the following: (1) how the market reacts to a designation using several event dates associated with the designation, (2) whether the characteristics of the firms that have been designated are different from those of the firms that have not been designated, and finally and most importantly, and (3) whether the financial reporting quality of the designated firm for unfaithful disclosure improves after a designation. Our investigation is motivated by the observation that conflicting predictions exist as to the consequences that ensue a designation. More specifically, some argue that a designation can carry severe penalties and sanctions including mandatory trading stoppage of the designated firm`s stock in the market for one day if the nature of a violation that led to the designation is deemed to be serious (i.e., the “significance scores” equal to or are higher than five using a scale that ranges from two to 12 points). Moreover, if the accumulated penalties over the past one year exceed 15 points, then the respective company will be put in the “watch” list by the stock exchange. Furthermore, if a violation is determined to be intentional or related to gross negligence, then the firm could be delisted from the exchange. Hence, the sanctions and penalties that accompany a designation might be strong enough to alter firms` behavior concerning disclosure in the post-designation period. Conversely, others observe that the practice of unfaithful disclosure not only persists but also increases in occurrence in the recent period. They interpret this as suggesting that firms do not fear to be designated for unfaithful disclosure perhaps because regulators are either unable or unwilling to impose significant enough sanctions for the violators. Mindful of these conflicting views about the consequences of an unfaithful disclosure designation, we empirically examine the designated firm`s disclosure behavior in the post-designation period. We find that the market reacts negatively to a designation announcement. More specifically, the market reaction to an announcement is negative during the announcement date of a potential designation for unfaithful disclosure and also during the announcement date of an actual designation for unfaithful disclosure. However, the market does not react to the occurrence of the reason that might result in the subsequent designation. Given that prior studies have documented the similar findings, our result is a replication of the findings in previous studies. We also find that the accounting quality of the designated firms is lower than others. More specifically, designated firms tend to show higher discretionary accruals measured in absolute value, lower predictive ability of earnings, and lower conservatism measured by the persistence of negative income. More importantly, we find that the financial reporting quality of the violators of the faithful disclosure requirement significantly improves after a designation, suggesting that the regulation successfully alter firms` behavior as it intends. We also find that the level of governance of the designated firm is on average lower than other firms. Although our work does not provide clues as to why the practice of unfaithful disclosure persists despite aforementioned potentially severe penalties, our finding does provide compelling evidence that an unfaithful disclosure designation alters the violators` disclosure behavior. Our finding contributes to the literature that examines the effect of regulation on firms` disclosure behavior.

여드름 환자에서 경구 Isotretinoin이 입술 피부장벽에 미치는 영향과 이에 대한 보습제의 예방 효과에 대한 연구

최재은 ( Jae Eun Choi ),서수홍 ( Soo Hong Seo ),손상욱 ( Sang Wook Son ),김일환 ( Il Hwan Kim ) 대한피부과학회 2008 대한피부과학회지 Vol.46 No.10

Background: Oral isotretinoin is an effective treatment for acne patients, and this agent may cause variable side effects. Among them, cheilitis is the most common symptom that occurs in virtually all these patients. Objective: We wanted to assess the functional changes of the lips of acne patients who were treated with oral isotretinoin by measuring the transepidermal water loss (TEWL), and we evaluated the benefit of using a moisturizing cream as an adjunctive treatment to oral isotretinoin. Methods: We measured the TEWL of the lips and cheeks in 17 acne patients who were treated with oral isotretinoin for 6 weeks. Then we compared the changes of the patients and the differences of the TEWL of the lip according to the dose of isotretinoin. In addition, we compared the TEWL change of the lips between 10 patients who were treated with oral isotretinoin and moisturizer cream (PHYSIOGEL(R) A.I. Cream) with that of 10 patients who received only isotretinoin. Results: The TEWL of the lip increased significantly during isotretinoin treatment while the TEWL of the cheek showed no noticeable increase. The increase of the TEWL of the lip was more definite in the 20 mg isotretinoin group than that of the 10 mg isotretinoin group. However, the TEWL of the lip remained stable in the patients treated with oral isotretinoin and moisturizer cream. Conclusion: The lips are vulnerable to the mechanism of isotretinoin due to the poor barrier function of the parakeratotic mucosal epithelium with incomplete corneocyte formation. Therefore, regular use of moisturizer on the lips is recommended as an adjunctive treatment along with oral isotretinoin. (Korean J Dermatol 2008;46(10):1353∼1356)