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Anabaena cylindrica 분해세균의 분리 및 동정
최영길,홍엽,신규철,김민성,한명수,Choi, Yong-Keel,Hong, Yup,Shin, Kyu-Chul,Kim, Min-Seong,Han, Myung-Soo 한국미생물학회 2002 미생물학회지 Vol.38 No.2
To isolate the bacteria lysing cyanobacteria, the sediment samples were collected from Dochang and Pal'tang Reservoir and Seokchon Lake. Each sample was smeared on the Anabaena cylindrica lawn and incubated in light chamber for 11 days. Bacteria having cyanobacteria-Iysing activity were isolated from the samples of Seokchon reservoir. Confirmation of cyanobacteria-Iysing activity was carried out to measure chlorophyll a and bacterial cell counting in mixed culture of Anabaena cylindrica and bacteria. Lysis was detected when extracellular meterials was added to the Anabaena cylindrica culture. The isolate was identified by analysis based on 16S rDNA sequence and morphological and physiological properties. The bacterial strain was taxonomically studied by the phylogenetic analysis based on 165 rDNA sequence. This strain was identified as a member of the genus Bacillus and designated as Bacillus sp. CHS1. 남조류 분해세균을 분리하기 위하여 도창, 팔당 저수지와 석촌 호수로부터 저층시료를 채취하였다. Anabaena cylindrica lawn에 각각의 시료를 도말한 후 11 일간 배양하였고 석촌 시료를 접중한 Anabaena cylindrica lawn에서 Anabaena cylindrica에 분해능을 가지는 세균을 분리하였다. 분해능 화인은 남조류와 분해세균을 혼합한 후 chlorophyll a간의 측정과 분리세균의 cell counting방법으로 확인하였고, 세포외 물질이 Anabaena cylindrica를 분해하는 것을 확인하였다. 분리균주는 16S rDNA 염기서열 분석과 형태학적, 생리학적 특징을 기초로 하여 동정하였다. 분리균주의 165 rDNA염기서열을 기초로한 분자계통학적 분석을 통하여 Bacillus속에 포함하는 계통학적 그룹에 속한다는 것을 확인하였고, Bacillus sp. CHSl으로 명하였다.
수계종속 영양세균 군집의 종조성 및 세포의 효소의 변화에 미치는 산성화의 영향
최영길(Yong Keel Choi),한명수(Myung Soo Han),김세화(Se Wha Kim),이경(Kyung Lee),유광일(Kwang Il Yoo) 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.1
N/A In an artificial pH-gradient batch culture system, the author analyzed the effects of acidification on the species composition of heterotrophic bacteria. As the result of this study, it was found that the numbers of total bacteria were not affected by acidification and that the population size of heterotrophic bacteria decreased as pH became lower. THe heterotrophic bacteria isolated from all of the pH gradient were 12 genera and 22 species, and among them, gram negative and gram positive bacteria were 64% and 36%, respectively. As pH decreased, the distribution rate of gram negative bacteria increased while that of gram positive bacteria decreased. Regarding to distribution rate of genuses in each pH gradient, 13 genuses appeared at pH 7 while only 5 genuses appeared at pH 3, which means that the diversity of genera decrease as pH decreased. The activities of extracellular enzyme showed the ranges of 0.008∼0.292μMl^-1hr^-1 in bioreactor system. The enzymatic activities decreased rapidly below pH 5 and then sustained 5∼38% at the lower pH values.
철원북방 DMZ 내의 중영양호 토교저수지의 생태학적 연구 5. 경안천 ( 팔당호 ) 과 토교저수지에서 식물플랑크톤의 크기별 현존량과 Chlorophyll α 의 계절 변동
한명수(Myung Soo Han),최영길(Yong Keel Choi),김세화(Se Wha Kim),이경(Kyung Lee),유광일(Kwang Il Yoo),이후랑(Hu Rang Lee),홍성수(Sung Su Hong),김영옥(Young Ok Kim) 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.1
N/A With physico-chemical environmental factors such as temperature, DO, pH, conductivity and nutrients, size fractionated of phytoplankton standing crops and chlorophyll α concentrations(>8㎛, 3∼8㎛ and <3㎛) and their relationships were investigated at each station of Kyungan Stream and Togyo Reservoir from April to November in 1997. The two sampling sites showed different nutrient status: Kyungan Stream was eutrophic, while Togyo Reservoir was mesotrophic. Large sizes of phytoplankton and chl. α were higher in Kyungan Stream, opposite to those of Togyo Reservoir; Standing crops of phytoplankton(>8㎛) and chl. α(3∼8㎛) were high in Kyungan Stream, while phytoplankton(3∼8㎛) and chl. α(<3㎛) were abundant in Togyo Reservoir. These results imply that phytoplankton community in the highly eutrophicated water mainly comprised the large filamentous and/or colonial algae, such as Microcystis spp. and Aphanizomenon flos-aquae, which easily enriched by nutrients loading.
안연준,한명수,민병례,최영길 ( Yeon Jun Ahn,Myung Soo Han,Byung Re Min,Yong Keel Choi ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2
To determine the successional level of soil ecosystem, microbial population was examined and environmental factors were measured. Sampling site were evergreen forests area. Among the environmental factors, total organic matter was ranged 11.880(Yesong-Ri, Wando-Gun, Jeoranam-Do)∼66.20㎎/g dried soil(Ggamak Island, Kangjin-Gun, Jeoranam-Do) in surface soil and 4.500(Mok Island, Uljoo-Kun Kyungsangbuk-Do)∼17.000㎎/g dried soi(Ggamak Island, Kangjin-Gun, Jeoranam-Do) in deep soil. Average total organic matter were 23.940 in surface soil and 11.988㎎/g dried soil in 15㎝ depth of soil. Soluble sugar content were measured from 0.045(Maengsun-ri, Wando-Gun, Jeoranam-Do) to 0.172㎎/g dried soil(Mira-ri, Wando-Gun, Jeoranam-Do) and average was 0.103㎎/g dried soil in surface. In 15㎝ depth of soil, it was ranged 0.019 (Maengwun-Ri, Wando-Gun, Jeoranam-Do)∼10.131㎎/g dried soil and average was 0.065㎎/g dried soil. On the basis of these experimental data, S/O value, the ratio of soluble sugar against total organic matter, in surface soil was ranged 0.002(Ggamak Island, Kangjin-Gun, Jeoranam-Do)∼0.095 (Jindo-Gun. Jeoranam-Do), and average was 0.005. And also it was ranged 0.001 (Maengsun-Ri, Wando-Gun. Jeoranam-Do) in lower depth of soil and average was 0.006. And all of S/O value were higher than 0.00371 in Mt. Palwang, 0.0018 in Mt. Weolchul and 0.0005 in Mt. Jukyeop. However, they were much lower than 0.0252 in Mt. Jiri and 0.16 in Mt. Kyebang. From the above result, it can be concluded that S/O value, the ratio of soluble sugar against total organic matter, in soil, was useful parameters for soil environment of climax forest in ecosystem.
팔당호의 생태학적 연구 - 2. 식물플랑크톤의 군집 구조의 변화 -
한명수,어윤열,유재근,유광일,최영길 ( Myung Soo Han,Yun Yeol Auh,Jae Keun Ryu,Kwang Il Yoo,Yong Keel Choi ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.3
In order to examine the changes in phytoplankton community structure in Pal`tang river-reservoir system and to describe some of their interrelations, 4 stations were chosen. Observations were carried out for four times at the Pal`tang reservoir and the lower part of three rivers such as North Han river, South Han river and Kyung-An stream from April 1992 to May 1993 and these data were analyzed with other published data. A total of 60 taxa in phytoplankton: of these, 24 were Bacillariophyceae, 29 were Chlorophyceae, five were Dinophyceae, the other taxa as Chrysophyceae, Cryptophyceae were identified one species, respectively. Rapid eutrophication contribute to the shift of the chief components of the total phytoplankton community from Bacillariophyceae to Bacillariophyceae including Chlorophyceae. Among them, Autacoseira(=Melosira) granulata dominated continuously in this study area since 1980. Changes in Phytoplankton community structure in Station 4(Pal`tang reservoir) affected by the longitudinal transport along each river strech in Pal`tang river-reservoir system. However, Changes of phytoplankton community structure probably were affected by its growth characteristics during the stagnation period of water column. Station 3(Kyung-An Stream) is heavily eutrophicated due to stagnation of water column.
수화발생 저수지로부터 남조류 분해능을 가지는 미생물의 분리
신규철(Kyu Chul Shin),한명수(Myung Soo Han),최영길(Yong Keel Choi) 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.1
N/A We have from water samples of Kwalim, Dochang, and Mulwang reservoirs in Kyonggi-Do, where cyanobacteria blooming occurred. Isolated microbes which have lytic activity for cyanobacteria. Water samples were ameared on the Anabaena cylindrica lawn and incubated in light chamber at 28℃, under 3000lux for 13 days. A fungus having cyanobacterial lytic activity was isolated from the samples of Dochang reservoir. The isolate was identified as Cryptococcus laurentii by Vitek system. From the culture of the isolate, four major extracellular protein bands(29, 35.2, 40.9, 51.1kDa) have been detected and the 29kDa protein band was more thickly appeared in the culture with cyanobacteria.
신규철(Kyu Chul Shin),서미연(Mi Yeon Suh),한명수(Myung Soo Han),최영길(Yong Keel Choi) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.4
Groundwater samples were collected at 6 sites in Seoul area. DNA extraction from the sample was performed by the boiling method. Samples were boiled with guanidinium thyocyanate and phenol-chloroform. One set of primer was designed for amplification fo 16S rDNA. For detection of denitrifying bacteria in groundwater sample, the author used primer sets consensus regions in gene sequences encoding the two forms of nitrite reductase(NIR), a key enzyme in the denitrification pathway. Two sets of PCR primer were designed to amplify cd_1-and Cu-nir. We confirmed the existence of denitrifying vacteria in 3 sites using cd_1-nir primer and in 4 sites using Cu-nir primer.
유독 중금속 오염물질 처리를 위한 미생물균주의 최근 이용 및 개발
방상원,최영길,한명수 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.2
There are several ways to remove and treat toxic heavy metals in the environment: chemical, physical and biological ways. The biological treatment utilizes the natural reactions of microorganisms living in the environments. These reactions include biosorption and bioaccumulation, oxidation and reduction, methylation and demethylation, metal-organic complexation and insoluble complex formation. The biological reactions provide a crucial key technology in the remediation of heavy metal-contaminated soils and waters. According to recent reports, various kinds of heavy metal species were removed by microorganisms and the new approaches and removal conditions to remediate the metals were also tried and reported elsewhere. This was mostly carried out by microorganisms such as fungi, bacteria and alga. In addition, a recent development of molecular biology shed light on the enhancing the microorganism's natural remediation capability as well as improving the current biological treatment.