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Rhizopus屬의 染色體에 關한 硏究(第二報) -II. Rhizopus 17種에 對하여-
민병례,Min, Byung-Re 한국미생물학회 1984 미생물학회지 Vol.22 No.3
After the previous paper, this chromosomal studies on the fungi were dealt with 17 species in genus of Rhizopus. The results are sumarized as the followings; The haploid chromosome number of 17 species were confirmed as of 6(Rh. oligosporus), 8(Rh. homothallicus, Rh. liquefaciens, Rh. shanghaiensis, Rh, acetorinus), 12(Rh. microsporus, Rh. pseudochinensis, Rh, rhizopodiformis, Rh, thermosus, and Rh. kazanensis), 14(Rh. stolonifer), and 16(Rh. suinus), respectively. Referring to the above fact and the previous paper, it is strongly presumed that the basic chromosome number of Rhizopus are 4.
Rhizopus oryzae와 Rh. microsporus균의 IGS 부위의 염기서열 분석
민병례 ( Byung Re Min ) 상명대학교 자연과학연구소 2005 自然科學硏究 Vol.15 No.-
The complete IGS(intergenic spacer) region sequence of Rhizopus oryzae and Rh. microsporus were analyzed to identify the source of its polymorphism and the presence or absence of some subrepeat motif in this genus. Using CNL12, CNS1 and several other primers IGS regions were amplified and analyzed the nucleotide sequence. The sizes of amplified IGS were 2555bp and 2807bp respectively. Very high level of sequence similarity with other fungi showed in 3`-28S end, 5-18S end and 5`-ETS region. Homologous subrepeat motif were not found, but some repeat motif observed : TTGTCTGT and CTCTCTGT in Rh. oryzae, ACCATTCACC and AAG in Rh. microsporus. The homology of nucleotide sequence between Rh. oryzae and Rh. microsporus was about 62%.
민병례 ( Byung Re Min ) 상명대학교 자연과학연구소 2005 自然科學硏究 Vol.15 No.-
A polymerase chain reaction random amplified polymorphic DNA(PCR-RAPD) was used to estimate the genetic relationship among twenty strains of the genus Rhizopus. Twelve 10-mer primers of arbitary nucleotide sequences were used for amplification of genomic DNA of Rhizopus spp. The size of amplified DNA fragments produced ranging from 2.5kb to 0.25kb. The results from PCR-RAPD analysis were grouped into four groups, Rh. oryzae, - Rh. stolonifer, Rh. microsporus - and Rh. homothallicus groups based on above 75% genetic similarity.