계획작품-삼척시 종합문화회관

최상용,Choe, Sang-Yong 대한건축사협회 1992 建築士 Vol.1992 No.4

Vitrification 방법에 의한 토끼수정란의 동결에 관한 연구

최상용,이영락,노규진,이효종,박충생,Choe, Sang-yong,Lee, Young-rak,Rho, Gyu-jin,Lee, Hyo-jong,Park, Choong-saeng 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3

The purpose of this study was to investigate the effects of developmental stage and equilibration time on survival of rabbit embryos following freezing by vitrification. Adult New Zealand White female rabbits were superovulated with PMSG and hCG. The 8-cell stage embryos were collected from 40 to 45 hours after hCG injection by flushing oviducts with Dulbecco's phosphated buffered saline and in vitro cultured in TCM-199 containing 10% fetal calf serum(FCS). Each embryos developed in vitro to 16-cell, compact morula and blastocyst was cryopreserved and cultured following thawing to examine their developmental potential to expanded blastocyst stage in vitro. The frozen-thawed-cultured embryos were stained with Hoechst 33342, and their nuclei were counted using a fluorescence microscope. On the toxicity test of EFS solution as cryopreservation, the survival rates of 8-cell stage embryos was decreased in reverse to increasing of exposure time over 5 minutes. The post-thaw survival rates of embryos on equilibration times was significantly(P<0.05) higher for 2 min. than for 5 or 10 minutes. From morula to blastocyst of rabbit embryos was more suitable than 8-cell stage for cryopreservation by vitrification. The higher post-thaw survival rate of embryos can be achieved by keeping the cryoprotectant at $4^{\circ}C$ than at $20^{\circ}C$. The mean number of nuclei per embryo following freezing by vitrification and in vitro culture to expanded blastocyst at compacted morula and blastcyst was not significantly differ from fresh blastocyst.

한우 난포란의 체외수정 및 체외수정란의 동결보존에 관한 연구

최상용,공일근,주영국,노규진,김용권,박충생,Choe, Sang-yong,Kong, Ill-keun,Joo, Young-kuk,Rho, Gyu-jin,Kim, Yong-kweon,Park, Choong-saeng 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.4

The ovaries of Korean Native cows or heifers were obtained from an abattoir and kept on 20 to $25^{\circ}C$ and transported to laboratory within 2 hrs. The follicular oocystes were collected from 2~6mm follicles in diameter and classified into 3 grades by the morphology of cumulus cells attached. The oocytes were matured in vitro(IVM) for 24 hrs. in TCM-199 supplemented with $23{\mu}g/ml$ FSH, $10{\mu}g/ml$ LH, $1{\mu}g/ml$ estradio-17 ${\beta}$ and granulosa cells at $39^{\circ}C$ under 5% $CO_2$ in air. They were fertilized in vitro(IVF) by incubation for 12 hrs. of epididymal spermatozoa pretreated with heparin, and then the zygotes were co-cultured in vitro(IVC) with oviductal epithelial cells for 7 to 9 days. Assessment of maturation revealed that 93.0%(147/158) of grade I oocytes had expanded of cumulus cells, which was higher(p<0.05) than the 79.4%(85/107) of grade II oocytes. Compared to epididymal sperm(32.9%), the insemination with frozen and thawed sperm resulted in slightly lower(20.5%), but not significant, development to morulae and blastocysts from grade I oocytes. Co-culture of bovine IVF embryos with oviductal epithelial cells improved the development to transferable embryos significantly(38.1%), compared to co-culture with granulosa cells(20.0%). When VF bovine embryos were vitrified at blastocyst, the post-thaw survival rate was obtained higher resulf for 1 min. equilibration time(82.6%) or 2 min.(73.9%) than 3 min.(18.2%) in EFS solution.

삼척시 종합문예회관 계획(안)

최상용,Choe, Sang-Yong 대한건축사협회 1991 建築士 Vol.1991 No.10

Studies on nuclear transplantation in mouse embryos. I. Functional differences between maternal and paternal genomes

최상용,박충생,이효종,박희성,Choe, Sang-yong,Park, Choon-saeng,Lee, Hyo-jong,Park, Hee-sung The Korean Society of Veterinary Science 1990 大韓獸醫學會誌 Vol.30 No.2

모성 및 부성 genome의 기능을 알아보기 위하여 미세조작기법과 Sendai virus를 이용한 핵융합 기술을 이용하여 2개의 자성전핵만으로 구성된 2배체의 gynogenetic 수정란을 그리고 2개의 웅성전핵만으로 구성된 2배체의 androgenetic 수정란을 인위적으로 작출하였다. 이들의 작출효율은 biparental 수정란에서는 56%, gynogenetic 수정란에서는 50% 그리고 androgenetic 수정란에서는 56% 이었다. 이들을 체외에서 배양한 결과 gynogenetic 및 androgenetic 수정란은 2-세포기 이후에는 biparental 및 intact 수정란에 비하여 그 발달능이 매우 저조하였으나 이들 중 25% 이상이 포배까지 발달한 수 있음을 확인하였다. Gynogenetic 및 androgenetic 수정란을 동기화된 수란생쥐의 난관내에 이식하였던 바, androgenetic 수정란은 전혀 착상 되지 않았으나, gynogenetic 수정란에서는 착상이 확인되었다. 핵이식기법으로 인위조작된 2배체의 biparental 수정란으로부터 28마리의 생쥐 신생자를 생산하였다. By nuclear transplantation technology twenty eight mice have been produced after transfer of heterozygous biparental eggs. Also heterozygous gynogenetic eggs with two female pronuclei and heterozygous androgenetic eggs with two male pronuclei have been obtained by injecting a male or female pronucleus with Sendai virus into the perivitelline space of enucleated haploid zygotes at pronuclear stage. The success rate of enucleation, karyoplast injection and fusion of both the pronuclei was 80.3, 83.4 and 81.8%, respectively. The overall pronuclei fusion rates by this technique were 56, 50 and 56% in biparental, gynogenetic and androgenetic eggs, respectively. The evidence was ascertained that the gynogenetic and androgenetic eggs were also able to develop in vitro up to blastocyst stage, even though their developmental potential was greatly diminished beyond 2-cell stage. The gynogenetic eggs were able to develop in vivo up to day 10 of pregnancy, while the androgenetic eggs failed to develop in vivo during the same period.

戰後 韓國民族主義의 問題狀況

崔相龍(Choe Sang-Yong) 한국정치학회 1976 한국정치학회보 Vol.10 No.-

한우에서 수송열의 예방을 위한 백신접종과 예방치료의 효과

최창용 ( Chang Yong Choe ),이명식 ( Myeong Sik Lee ),나승환 ( Seung Hwan Na ),정영훈 ( Young Hun Jung ),윤순식 ( Soon Seek Yoon ),최상용 ( Sang Yong Choe ),나기준 ( Kie Jun Na ) 한국임상수의학회 2000 한국임상수의학회지 Vol.17 No.1

Studies on nuclear transplantation in mouse embryos II. Developmental potential of nuclei from embryos of different developmental stages

박충생,최상용,이효종,박희성,Park, Choong-saeng,Choe, Sang-yong,Lee, Hyo-jong,Park, Hee-sung The Korean Society of Veterinary Science 1990 大韓獸醫學會誌 Vol.30 No.4

Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos by micromanipulation and Sendai virus mediated fusion. The developmental potential of these reconstituted embryos in vitro and in vivo was examined. It was found that the single nuclei which were transplanted to enucleated two-cell embryos were not only able to develop to the blastocyst stage in vitro(two-cell nuclei, 76.5%; four-cell nuclei, 68.4%; eight-cell nuclei, 48.3%), but also able to develop to full term in vivo after transfer to recipient mice(two-cell nuclei, 37.1%; four-cell nuclei, 29.6%; eight-cell nuclei, 16.3%). Although the proportion of live young produced after transfer of nucler of nuclear transplant embryos which received eight-cell nuclei was significantly (p<0.05) reduced, it would be suggested that the overall efficiency in producing identical offspring is greater when eight-cell embryos were selected for nuclear donor than two- or four-cell embryos were selected. 포유동물의 초기 발생단계에서 핵의 분화와 전능성(totipotency) 을 규명하고, 수정란의 cloning technique를 개발하여 우량유전자로 조성된 개체를 복제함으로써 효과적인 종축개량 기법으로 응용하기 위하여 생쥐 수정란을 모델로 하여 미세조작기법과 Sendai virus를 이용한 핵융합기술을 이용하여 인위적으로 동일한 유전자를 가진 복제 수정란을 작출하고 이들의 작출효과, 체외발달능력 및 체내 이식후 개체발생여부 등을 조사하였다. 2-세포기, 4-세포기 및 8-세포기의 수정란으로부터 핵을 채취하여 이들을 탈핵된 2-세포기의 수정란에 이식하였을 때, 이들의 핵융합 성공율은 각각 88.6%, 87.1% 및 84.7%이었다. 나아가서 이들 핵융합된 수정란을 체외에서 96시간 배양한 결과, 2-세포기, 4-세포기 및 8-세포기의 핵이 이식된 수정란은 각각 76.5%, 68.4% 및 48.3%가 배반포로 발달하였다. 핵이식 후 체외에서 배반포로 발달된 수정란을 골라 수란생쥐에 이식하였던 바, 2-세포기의 핵이 이식된 수정란 156개 중 58개(37.1%) 가 발달하여 신생자로 생산되었으며, 4-세포기의 핵아 이식된 수정란 135개 중 40개(29.6%)가, 그리고 8-세포기의 핵이 이식된 92개의 수정란 중 15개(16.3%)가 신생자로 생산되었다.

장결석증으로 폐사한 말의 병리학적 소견

김순복,최상용,Kim Soon-Bok,Choe Sang-Yong 대한수의사회 1984 대한수의사회지 Vol.20 No.5

Pathological findings and its possible pathogenesis in a race horse dying from enterolith were described. Clinical signs were abdominal pain, anorexia, diarrhea, recumbency, and collapse. Gross lesions consisted mainly of failure to blood clot, peritoniti 장결석증으로 폐사한 경주말의 병리학적 소견과 이들의 발생기전을 기술하였다. 임상적으로는 선통, 식욕부진, 하리, 횡와 및 허탈을 주증으로 하였고, 육안적으로는 혈액응고불양, 복막염, 각종 장기의 충출혈과 소결장의 기시부를 직경 14.4cm의 광물성 결석이 폐쇄하고 있는 것을 볼 수 있었으며, 상부대결장에서는 확장과 천공이 인정되었다. 현미경적 소견으로는 간장, 신장 및 부신의 변성괴사, 산재성 혈전, 폐수종, 그리고 대결장에서 습성괴저를 관찰할 수 있었다. 이상의 소견들은 장벽의 빈혈성괴사 및 괴저, 그리고 복막염 등에 기인하는 자가중독성 및 내독소성 독혈증에 의한 것이라고 생각된다.

한국재래산양(韓國在來山羊)의 태아(胎兒) 및 신생자(新生仔)의 체적측정치(體尺測定値)에 관(關)한 연구(硏究)

김종섭,최상용,정헌식,김택석,Kim, Chong-sup,Choe, Sang-yong,Chung, Hyon-sik,Kim, Taeg-seog 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

The measurement was investigated with 18 heads of fetus(60, 90, 120 days of gestation) and neonate in Korean native goats. The results were summerized as follows: 1. The crown-rump length of fetuses at 60, 90, 120 days of gestation and neonate was 8.71, 20.83, 31.10 and 34.93 cm, respectively. 2. The length of small intestine at 60, 90, 120 days of gestation and neonate was 32.28, 157.10, 303.52 and 457.06 cm, respectively. 3. The length of large intestine at 60, 90, 120 days of gestation and neonate was 9.20, 37.70, 82.06 and 94.46 cm, respectively. 4. The ratio of intestinal length to crown-rump length at 60, 90, 120 days of gestation and neonate was 4.76, 9.45, 12.40 and 15.79 times, respectively. 5. At 60 days of gestation, the total length of the vertebral column was $7.40{\pm}0.72cm$, The mean length of each segment of the vertebral column was $1.55{\pm}0.20cm$ in cervical, $2.29{\pm}0.21cm$ in thoracic, $1.46{\pm}0.10cm$ in lumbar, $0.51{\pm}0.04cm$ in sacral and $1.59{\pm}0.17cm$ in coccygeal vertebrae. 6. At 90 days of gestation, the total length of the vertebral column was $16.52{\pm}0.80cm$. The mean length of each segment of the vertebral column was $3.72{\pm}0.12cm$ in cervical, $5.09{\pm}0.26cm$ in thoracic, $3.22{\pm}0.04cm$ in lumbar, $1.97{\pm}0.03cm$ in sacral and $2.64{\pm}0.35cm$ in coccygeal vertebrae. 7. At 120 days of gestation, the total length of the vertebral column was $26.35{\pm}0.34cm$. The mean length of each segment of the vertebral column was $6.09{\pm}0.16cm$ in cervical, $7.81{\pm}0.07cm$ in thoracic, $5.08{\pm}0.07cm$ in lumbar, $3.07{\pm}0.02cm$ in and $4.31{\pm}0.02cm$ in coccygeal vertebrae. 8. In the neonate, the total length of the vertebral column was $32.41{\pm}1.57cm$. The mean length of each segment of vertebral was $7.70{\pm}0.25cm$ in cervical, $9.97{\pm}0.68cm$ in thoracic, $5.58{\pm}0.44cm$ in lumbar, $3.85{\pm}0.15cm$ in sacral and $5.05{\pm}0.06cm$ coccygeal vertebrae. 9. The chest girth at 60, 90, 120 days of gestation and neonate was $6.13{\pm}0.51$, $13.45{\pm}0.84$, $20.28{\pm}1.53$ and $22.94{\pm}1.75cm$, respectively. 10. The head length at 60, 90, 120 days of gestation and neonate was $2.93{\pm}0.07$, $6.67{\pm}0.13$, $8.84{\pm}0.51$ and $9.76{\pm}0.44cm$, respectively. 11. The width of the head at 60, 90, 120 days of gestation and neonate was $2.20{\pm}0.13$, $4.45{\pm}0.11$, $5.33{\pm}0.20$ and $5.51{\pm}0.32cm$, respectively.