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결핵성 종격동염에 병발되어 종격동 농양을 일으킨 Eikenella Coorodens 감염 1예
최강원,우준희,송영욱,한성구,김건열,한용철,유철규 대한감염학회 1988 감염 Vol.20 No.3
Eikenella corrodens is a slow-growing, gram-negative, facultative, anaerobic organism that is normally found among oropharyngeal flora. It is frequently isolated in mixed culture. Consequently, E. corrodens may be missed as a pathogen, for it may be overgrown or fail to grow on routine culture. But its isolation as a pathogen is increasingly being reported. It has been recovered as the sole infecting agent in cases of endocarditis, subdural empyema, meningitis, osteomyelitis, and skin abscess. We report a case of E. corrodens infection which caused mediastinal abscess in association with tuberculous mediastintis.
Ambidentate 리간드의 금속착물 (제 8 보). Isonitrosomethylacetoacetate Imine 리간드의 니켈(II) 및 팔라듐(II) 착물
구본창,최강열,이만호,김인환,Bon-Chang Goo,Gang-Yeol Choi,Man-Ho Lee,In-Whan Kim 대한화학회 1993 대한화학회지 Vol.37 No.7
isonitrosomethylacetoacetate(H-IMAA)의 이민유도체를 리간드로하는 새로운 Ni(II) 및 Pd(II)착물, Ni(IMAA-NH)(IMAA-NH'), Ni(IMAA-NH)(IMAA-NR),$ Pd(IMAA-NH)_2$, 및 $Pd(IMAA-NR)_2(R=CH_3,\;n-C_3H_7,\;n-C_4H_9$, 또는 $CH_2C_6H_5)$을 합성하고, 이들 착물의 구조를 원소분석, 전자흡수분광법, 적외선흡수분광법, $^1H$ 및 $^{13}C$ 핵자기공명분광법으로 조사하였다. 여기서 H-IMAA-MH 및 H-IMAA-NR은 각각 isonitrosomethylacetoacetate imine 및 N-alkylisonitrosomethylacetoacetate imine 유도체를 나타낸다. New Ni(II) and Pd(II) complexes of isonitrosomethylacetoacetate imine derivatives, Ni(IMAA-NH)(IMAA-NH'), Ni(IMAA-NH)(IMAA-NR), $Pd(IMAA-NH)_2\;and Pd(IMAA-NR)_2(R=CH_3,\;C_2H_5,\;n-C_3H_7,\;n-C_4H_9,\;or\;CH_2C_6H_5)$, where H-IMAA-NH and H-IMAA-NR represent isonitrosomethylacetoacetate imine and N-alkylisonitrosomethylacetoacetate imine derivative, respectively, have been prepared and the structures of the complexes have been studied by elemental analyses, electronic, infrared, and $^1H-\;and\;^{13}C-NMR$ spectroscopies.
박미희,최강열,민도식 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-
Endocytosis is differentially regulated by hypoxia-inducible factor-1α (HIF-1α) and phospholipase D (PLD). However, the relationship between HIF-1α and PLD in endocytosis is unknown. HIF-1α is degraded through the prolyl hydroxylase (PHD)/von Hippel–Lindau (VHL) ubiquitination pathway in an oxygen-dependent manner. Here, we show that PLD1 recovers the decrease in epidermal growth factor receptor (EGFR) endocytosis induced by HIF-1α independent of lipase activity via the Rab5-mediated endosome fusion pathway. EGF-induced interaction of PLD1 with HIF-1α, PHD and VHL may contribute to EGFR endocytosis. The pleckstrin homology domain (PH) of PLD1 itself promotes degradation of HIF-1α, then accelerates EGFR endocytosis via upregulation of rabaptin-5 and suppresses tumor progression. These findings reveal a novel role of the PLD1-PH domain as a positive regulator of endocytosis and provide a link between PLD1 and HIF-1α in the EGFR endocytosis pathway.
Isonitrosoethylacetoacetate Imine 리간드의 니켈(II) 착물 합성 및 구조 특성
김인환,구본창,최강열,김상태,이만호 大邱大學校附設 基礎科學硏究所 1993 基礎科學硏究 Vol.10 No.-
Nickel(lI) Complexes of the type Ni(IEAA-NH)(IEAA-NR) (R = H₁, CH₃, C₂H_(5), n-C₃H_(7), n-C₄H_(9), or CH₂C_(6)H_(5)), where IEAA-NH and IEAA-NR represents isonitrosoethylacetoacetate imine and its N-alkyl derivative, respectively, have been prepared. And the structure of the complexes have been studied by elemental analyses, electronic, infrared,1H and 13C-NMR spectra. It has been determined that IEAA-NH ligand coordinates to nickel through the nitrogen of imine group and the oxygen of isonitroso group to form 6-membered chelate ring and IEAA-NR coordinates to nickel through the nitrogens of imino group and isonitroso group to form 5-membered chelate ring in the square-planar Ni(IEAA-NH)(IEAA-NR) complexes.
역상 액체 크로마토그래피에 의한 Ni(Ⅱ)-Isonitrosoethylacetoacetate Imine 유도체 킬레이트의 용리 메카니즘에 관한 연구
李垣,李萬浩,최강열,金仁煥,姜昌禧 濟州大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.6 No.1
역상 액체 크로마토그래피에 의해서Ni(IEAA-NH)(IEAA-NR)(R-H, CH₃, C₂???, n-C₃???, n-C₄????, ??????-CH₂)킬레이트의 동시 분리 및 분석을 위한 최적조건을 Micropak MCH-5 분리관을 사용하여 조사하고, 이들의 용리거동에 대한 인자들과의 관계를 검토하였다. 용리액으로 methanol/water의 이성분 용매를 사용하였으며, 모든 금속 킬레이트의 용매 세기 인자는 0≤logk'≤1의 범위임을 확인하고, 각 금속 킬레이트의 logk'값과 k'값을 도시하여 본 결과 직선 관계가 성립됨으로써 금속 킬레이트의 용리 메카니즘이 주로 소용매성 효과 즉 소수성 효과에 기인함을 확인하였다. 한편 금속 킬레이트의 머무름에 미치는 용량 인자와 온도와의 관계를 조사한 결과 좋은 직선성을 나타내고 있으며, 뿐만 아니라 직선의 기울기로부터 엔탈피(△H)와 엔트로피(△S)를 구하고, 엔탈피와 용량 인자와의 값을 도시한 결과도 직선관계가 성립하였다. 직선의 기울기로부터 구한 보정 온도(β)는 773.47。K이었다. 이 결과 역상HPLC에서 Ni(Ⅱ)-Isonitrosoethylacetoacetate imine 킬레이트의 용리 메카니즘의 주로 소수성 효과에 기인함을 재확인 할 수 있었다. Liquid Chromatographic behavior of Ni(Ⅱ)-Isonitrosoethylacetoacetate Imine(IEAA-NR), Ni(IEAA-NH)(IEAA-NR)(R=H, CH₃, C₂???, n-C₃???, n-C₄????, ??????-CH₂) chelates were investigated by reversed-phase HPLC on Micropak MCH-5 colulmn using methanol/water as mobile phase. The optimum conditions for the separation of Ni(IEAA-NH)(IEAA0NR) chelates were examined with respect to the effect of the flow rate, sample solvent, mobile phase strength and column temperature. It was found that metal chelates were properly eluted in an acceptable range of capacity factor value (0≤logk'≤1). The dependence of the logarithm of capacity factor (k') on the volume fraction of water in the binary mobile phase as well as on the liquid-liquid extraction distribution ratio (Dc) in methanol-water/n-alkane extraction system showed the good linearties, and the dependence of the logarithm of capacity factor (k') on the column temperature and on the enthalpy exhibited the good linearties, and the compensation temperature (β) from the slope was 773.47。K. It was suggested that the retention of metal chelates was largely affected by the hydrophobic effect.
Phospholipase D2 promotes degradation of hypoxia-inducible factor-1α independent of lipase activity
박미희,배순식,최강열,민도식 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-
Hypoxia-inducible factor-1α (HIF-1α) is a key transcriptional mediator that coordinates the expression of various genes involved in tumorigenesis in response to changes in oxygen tension. The stability of HIF-1α protein is determined by oxygen-dependent prolyl hydroxylation, which is required for binding of the von Hippel-Lindau protein (VHL), the recognition component of an E3 ubiquitin ligase that targets HIF-1α for ubiquitination and degradation. Here, we demonstrate that PLD2 protein itself interacts with HIF-1α, prolyl hydroxylase (PHD) and VHL to promote degradation of HIF-1α via the proteasomal pathway independent of lipase activity. PLD2 increases PHD2-mediated hydroxylation of HIF-1α by increasing the interaction of HIF-1α with PHD2. Moreover, PLD2 promotes VHL-dependent HIF-1α degradation by accelerating the association between VHL and HIF-1α. The interaction of the pleckstrin homology domain of PLD2 with HIF-1α also promoted degradation of HIF-1α and decreased expression of its target genes. These results indicate that PLD2 negatively regulates the stability of HIF-1α through the dynamic assembly of HIF-1α, PHD2 and VHL.