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국내산 구아바(Psidium guajava L.) 잎 추출물의 항산화 활성 및 간세포 보호효과
천원영,서동연,김영화,Cheon, Wonyoung,Seo, Dongyeon,Kim, Younghwa 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.1
The purpose of this study was to evaluate the antioxidant and hepatocyte protective effects of guava (Psidium guajava L.) leaves cultivated in Korea. The contents of the total polyphenol of the extract was 271.57 mg gallic acid equivalent (GAE)/g residue. Antioxidant activities of leaf extract were evaluated by examining the free radical scavenging ability. 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ${\alpha}-{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) free radical scavenging activities of the extract were 1133.23 mg trolox equivalent antioxidant capacity (TEAC)/g residue and 721.68 mg TEAC/g residue, respectively. The hepatocyte protective effect of guava leaf extract was examined in HepG2 cells. Against tert-butyl hydroperoxide (TBHP), the viability of HepG2 cells were increased by the treatment of leaf extract. In addition, guava leaf extract led to the inhibition of reactive oxygen species (ROS) generated in HepG2 cells. The leaf extract increased the activity of glutathione (GSH), glutathione reductase (GR), and glutathione peroxidase (GPx) against oxidative stress. These results suggested that guava leaves might be regarded as a potential source natural antioxidant and a hepatoprotective material.
권지현,천원영,이상훈,최용민,김영화 한국식생활문화학회 2020 韓國食生活文化學會誌 Vol.35 No.3
In this study, biotin (vitamin B7) contents of frequently consumed foods in Korea were determined by usingimmunoaffinity column in conjunction with high-performance liquid chromatography (HPLC). The biotin contents of 24foods of plant origin and 27 foods of animal origin were selected. The highest biotin contents in frequently consumed foodsof plant origin were found in red beans (Huinguseul; 11.475 μg/100 g). On the other hand, biotin was not detected in anyvarieties of sorghum. For frequently consumed foods of animal origin, salted pollack roe (7.486 μg/100 g) showed thehighest biotin content. However, beef and fish contained less biotin. All biotin analyses were conducted under analyticalquality control. The limits of detection and limits of quantification of biotin were 0.007 and 0.023 μg/100 g, respectively, andthe accuracy/recovery percentage was 95.35-105.02%. The precision values were 4.041% (repeatability) and 3.835%(reproducibility). Taken together, our data provide reliable data on the biotin contents of frequently consumed foods inKorea.
산화적 스트레스에 대한 여주(Momordica charantia Linn.)잎의 항산화 활성 및 간세포 보호능
전아영,천원영,윤지민,김대중,김영화,Jeon, Ahyeong,Cheon, Wonyoung,Yoon, Jimin,Kim, Dae-Jung,Kim, Younghwa 한국식생활문화학회 2020 韓國食生活文化學會誌 Vol.35 No.6
This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantia Linn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g, respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations of ethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mg cathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed the highest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. The E100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extract showed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactive oxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.