하위 적엽처리가 딸기의 생육 및 생산성에 미치는 영향

이규빈,이정은,제병일,이용재,박영훈,최영환,손병구,강남준,강점순,Lee, Gyu-Bin,Lee, Jung-Eun,Je, Byoung-Il,Lee, Yong-Jae,Park, Young-Hoon,Choi, Young-Whan,Son, Beung-Gu,Kang, Nam-Jun,Kang, Jum-Soon 한국환경과학회 2020 한국환경과학회지 Vol.29 No.3

The purpose of this study was to investigate the effects of defoliation treatment on the growth and yield of strawberries. There was a remarkable growth in the above-ground part and root of untreated strawberry plants possibly due to higher amount of photosynthesis, while overall plant growth was suppressed as the level of defoliation treatment increased. In both the "Seolhyang" and "Maehyang" cultivars examined, defoliation treatment resulted in small fruits and a low number of fruits per plant. Notably, 50% defoliation significantly reduced the number of fruits per plant to 8.2, compared to 13.8 in untreated plants. Defoliation treatment also negatively influenced the fruit quality including color, sugar content, and solid-acid rate. However, no significant changes in fruit firmness was observed in either cultivar. Therefore, retaining enough leaves without defoliation treatment can be important to increasing fruit yield, producing high quality fruits and saving labor required for defoliation.

광부족 조건이 딸기의 생육 및 생산성에 미치는 영향

이규빈,이정은,제병일,이용재,박영훈,최영환,손병구,강남준,강점순,Lee, Gyu-Bin,Lee, Jung-Eun,Je, Byoung-Il,Lee, Yong-Jae,Park, Young-Hoon,Choi, Young-Whan,Son, Beung-Gu,Kang, Nam-Jun,Kang, Jum-Soon 한국환경과학회 2020 한국환경과학회지 Vol.29 No.2

The purpose of this study was to investigate the effect of shading on the growth and productivity of strawberries. Photosynthesis was normally achieved under natural light without shading treatment, and vegetative growth of under- and aboveground part of strawberry plants were excellent. Strawberry fruit productivity and quality were different depending on shading conditions. In natural light, they were improved possibly by balanced vegetative and reproductive growth. However, under light-shading conditions with insufficient sunshine, photosynthetic activity deteriorated and carbohydrate production was therfore inadequate; this adversely affected plant height and quality. The negative effects were more pronounced at 50% shading condition. Shading treatments resulting in insufficient sunshine had a detrimental impact on plant productivity and growth; this implies that proper shading conditions could heip improve yield and fruit quality.

발아온도 및 Spermine, Putrescine이 딸기의 화분 발아에 미치는 영향

이정은,김현도,제병일,이용재,박영훈,최영환,손병구,강남준,강점순,Lee, Jung-Eun,Kim, Hyeon-Do,Je, Byoung-Il,Lee, Yong-Jae,Park, Young-Hoon,Choi, Young-Whan,Son, Beung-Gu,Kang, Nam-Jun,Kang, Jum-Soon 한국환경과학회 2020 한국환경과학회지 Vol.29 No.4

This study was conducted to determine the optimum conditions for the treatment of polyamine to promote pollen germination and improve the fruiting rate by overcoming fertilization defects in low-temperature strawberry cultivation. The optimum temperature for pollen germination of strawberries was 30 ℃ for Seolhyang and 25 ℃ for Maehyang, and Seolhyang had a higher pollen germination rate than that of Maehyang. The addition of polyamines, namely spermine and putrescine to the medium improved the pollen germination rate and spermine treatment showed a better effect than putrescine treatment. The proper polyamine type and treatment concentration for enhancing the potency of pollen germination was 500 μM of spermine for both Seolhyang and Maehyang, which improved the pollen germination rate by 19-23% compared with that of the control. However, combined treatment of spermine and putrescine, resulted in a lower germination rate lower than that of the single treatment. Our results indicated that the treatment of polyamines during flowering in protected cultivation of strawberrise can improve the fruiting rate by overcoming the problem of poor pollen germination due to low temperature.

수박 엘리트 계통의 GBS를 통한 마커이용 육종용 SNP 마커 개발

이준우,손병구,최영환,강점순,이용재,제병일,박영훈,Lee, Junewoo,Son, Beunggu,Choi, Youngwhan,Kang, Jumsoon,Lee, Youngjae,Je, Byoung Il,Park, Younghoon 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.3

본 연구는 국내 육종 회사에서 개발된 수박(Citrullus lanatus L.) 우량 육성계통 20종을 대상으로 Genotyping-by-sequencing(GBS) 분석을 통해 품종식별, 순도검정, 그리고 마커이용여교잡(Marker-assisted backcross, MABC)용 SNP 세트를 개발하고자 수행되었다. GBS 분석 결과 총 1,100,000천개 raw read 중 77%가 수박 유전체에 mapping되었으며 평균 mapping region은 약 4,000 Kb로 2.3%의 genome coverage를 보였다. Filtering을 통해 평균 depth 31.57의 SNP 총 2,670개를 얻었으며, 20개 계통에 대한 이들의 Polymorphic information content(PIC) 값의 범위는 0.1 ~ 0.38 였다. 이 중 PIC 값이0.3이상이며 각 염색체 별로 5개씩 균등히 분포된 SNP 총 55개를 최종 선발하였다. 사용된 20개 계통의 유연관계분석을 위해 선발된 55개 SNP를 기반으로 한 주성분 분석(Principle component analysis, PCA) 결과 주성분 1 (52%)과 주성분 2 (11%)를 기준으로 4개의 그룹으로 분류 되었으며 각 계통 간 유전자형에 따른 뚜렷한 식별이 가능하였다. 계층적 군집화(Hierarchical clustering) 분석에서도PCA에서와 유사한 분류양상을 관찰할 수 있었다. 따라서 본 연구에서 개발된 SNP 세트는 적용 가능성이 검증된 20개 계통뿐 만 아니라 향후 다양한 수박 육종소재 및 품종에 대한 품종식별, F1 순도검정 및 MABC에 활용될 수 있으리라 기대된다. This study was conducted to develop an SNP set that can be useful for marker-assisted breeding (MAB) in watermelon (Citrullus. lanatus L) using Genotyping-by-sequencing (GBS) analysis of 20 commercial elite watermelon inbreds. The result of GBS showed that 77% of approximately 1.1 billion raw reads were mapped on the watermelon genome with an average mapping region of about 4,000 Kb, which indicated genome coverage of 2.3%. After the filtering process, a total of 2,670 SNPs with an average depth of 31.57 and the PIC (Polymorphic Information Content) value of 0.1~0.38 for 20 elite inbreds were obtained. Among those SNPs, 55 SNPs (5 SNPs per chromosome that are equally distributed on each chromosome) were selected. For the understanding genetic relationship of 20 elite inbreds, PCA (Principal Component Analysis) was carried out with 55 SNPs, which resulted in the classification of inbreds into 4 groups based on PC1 (52%) and PC2 (11%), thus causing differentiation between the inbreds. A similar classification pattern for PCA was observed from hierarchical clustering analysis. The SNP set developed in this study has the potential for application to cultivar identification, F1 seed purity test, and marker-assisted backcross (MABC) not only for 20 elite inbreds but also for diverse resources for watermelon breeding.

이상 고온 조건이 딸기의 생육, 수량 및 생리활성 성분에 미치는 영향

이규빈 ( Gyu-bin Lee ),이정은 ( Jung-eun Lee ),제병일 ( Byoung-il Je ),이용재 ( Yong-jae Lee ),박영훈 ( Young-hoon Park ),최영환 ( Young-whan Choi ),손병구 ( Beung-gu Son ),강남준 ( Nam-jun Kang ),강점순 ( Jum-soon Kang ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.1

In this study, we investigated the influences of abnormal high temperature on growth, yield and physiologically active substances of the strawberry. General strawberry cultivars in the 20℃ growth condition showed much better growth of leaf number, length, diameter along with plant height, compared with those in 22.5℃ or 25℃. But the cultivars of both ‘Sulhyang’ and ‘Mehyang’ showed good growth and development at 25℃ with the roots showing great growth at 20℃. The quality and yield of the strawberry were best in the 20℃ growth condition, but the merchantability deteriorated in the 25℃ high temperature condition. As for the content of the physiologically active substances of the strawberry, it increased at 20℃, the optimum growth temperature, but decreased at 25℃. The physiologically active substances in the strawberry differed among the cultivars, the contents of cyanidin-3-glucoside, cinchonine, ellagic acid and cinnamic acid higher in the ‘Mehyang’, whereas the content of fisetin is higher in the ‘Sulhyang’ cultivar.Consequentially, the high temperature in summer has a negative effect on the physiological active ingredients of the strawberry, which was increased in the strawberry cultivated at proper temperature, and high quality strawberry production was possible.

꾸지뽕 신초 엽위별 잎 추출물의 항비만 효과

박주하(Ju Ha Park),궈루(Lu Guo),강혜미(He Mi Kang),손병구(Beung Gu Son),강점순(Jum Soon Kang),이용재(Yong Jae Lee),박영훈(Young Hoon Park),제병일(Byoung Il Je),최영환(Young Whan Choi) 한국생명과학회 2021 생명과학회지 Vol.31 No.2

본 연구는 꾸지뽕 신초에 부착된 잎을 6단계 즉 정아의 잎(L0), 정아부에서 하부의 순서대로 L1, L2, L3, L4 및 기부의 잎을 L5로 분류하여 채취하였다. 신초의 위치에 따라서 분류한 잎을 70% 에틸알콜로 추출한 다음 3T3-L1 cell line을 이용하여 지방분화 억제 효과를 스크린하고, 가장 효과가 좋았던 꾸지뽕 신초 정단잎(CTL0) 추출물의 작용기작을 규명하였다. 꾸지뽕 신초 정단잎(CTL0) 추출물의 지방분화 억제효과가 가장 좋았으며, 신초의 중간 잎인 CTL2까지는 정단 잎에서부터 중간 잎 추출물일수록 효과가 감소하였으며, 신초의 중간 하부 잎인 CTL3부터 기부 잎인 CTL5 추출물은 100 ㎍/ml의 고농도에서 효과가 없었다. 꾸지뽕 신초 정단잎(CTL0) 추출물을 12.5, 25 및 50 ㎍/ml의 농도로 처리하여 Oil Red O를 분석한 결과, 농도 의존적으로 3T3-L1세포의 지방분화를 억제시켰다. 3T3-L1 세포에 지방분화물질인 insulin, dexamethasone 및 rosiglitazone을 첨가한 분화배지(DM)에서는 배지내에 glucose의 함량이 낮았으나, CTL0 추출물의 처리는 배지에 glucose함량이 많이 남아있었기 때문에 세포내로 glucose의 흡수를 억제시켰으며, 세포내 중성지방의 함량도 감소하였다. CTL0처리는 전구지방세포에서 지방세포로 분화될 때에 주요 전사인자로 알려진 PPARγ와 PPARγ의 target 유전자인 LPL, A-FABP 및 Glut4 단백질의 발현을 농도 의존적으로 억제하였다. 따라서 꾸지뽕 신초 정단잎(CTL0) 추출물은 항비만 효과가 우수한 소재로서 활용가치가 높을 것으로 사료된다. This study aimed to evaluate the anti-obesity effects of Cudrania tricuspidata leaf extract in the order of leaf development on the shoot (L0, L1, L2, L3, L4, and L5). The leaves at the apex of a Cudrania tricuspidata shoot were classified as L0; the next leaves of the apex were classified as L1, L2, L3, and L4 from highest to lowest; and the lowest leaf was classified as L5. A series of 70% ethyl alcohol leaf extracts were screened for the inhibitory effects of adipogenesis in 3T3-L1 preadipocytes. We found that the apical leaf extract of Cudrania tricuspidata (CTL0) was the most effective. Next, a study was conducted on the inhibitory action mechanism of CTL0. Treatment with CTL0 significantly suppressed the differentiation of 3T3-L1 preadipocytes in a dose-dependent manner, as confirmed by the decrease in lipid droplet content observed with Oil Red O staining. Treatment with 12.5 ㎍/ml, 25 ㎍/ml, and 50 ㎍/ml of CTL0 significantly reduced the lipid droplet content. Glucose and cellular triglyceride concentrations were reduced in the 3T3-L1 cells on the CTL0-treated medium compared to the differentiation medium (DM control, DMEM + insulin + dexamethasone + rosiglitazone). Compared with DM, CTL0 significantly inhibited the expression of key pro-adipogenic transcription factors, including peroxisome proliferator-activated receptor γ (PPARγ), LPL, A-FABP, and Glut4. These findings show that CTL0 extract has potent anti-obesity effects.