Glucose Oxidase에 의해 손상된 배양된 신경아세포에 미치는 국화 추출물의 효과

제갈승주,서영미,박승택,임요섭,정옥봉 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.2

To clarify the oxidative stress of reactive oxygen species (ROS) and the effect of Chrysanthemum morifolium L. (CM) flower extract on the cultured neuroglial cells (C6 glioma) damaged by ROS, cell adhesion effect was measured by colorimetric assay after cultured C6 glioma cells were treated with various concentrations of glucose oxidase (GO) for 5 hours. For the antioxidative effect of CM flower extract, cell adhesion activity (CAA), superoxide dismutase (SOD)-like activity and lactate dehydrogenase (LDH) activity were assessed against GO-induced cytotoxicity on same cultures. In this study, GO remarkably decreased CAA dose-dependently, and the XTT90 and XTT50 values were measured at 15 mU/mL and 50 mU/mL following the treatment of C6 glioma cells with 5∼60 mU/mL of GO. The CM flower extract significantly increased cell adhesion activity damaged by GO-induced cytotoxicity, and it also showed the SOD-like activity and the decrease of LDH activity. From these results, it is suggested that GO was cytotoxic on cultured C6 glioma cells, and CM flower extract showed antioxidative effects as shown by the increased CAA, SOD-like activity and the decrease of LDH activity on GO-induced cytotoxicity on the same cultures. .

Gallocyanin양료(梁料)를 리용(利用)한 세포핵양색(細胞核梁色)과 응용(應用)

제갈승주,오근영 대한임상검사과학회 1978 대한임상검사과학회지(KJCLS) Vol.10 No.1

사염화탄소, Dimethylnitrosamine, Thioacetamide의 3가지 시약과 쥐에게 간섬유증을 유도하는데 이용되는 담관결찰로 인한 조직병리학적 변화의 비교

제갈승주,김정훈,박미정,김진영,신진희,박수영 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.4

This study was carried out to compare the histopathological differences of liver lesions in carbon tetrachloride (CCI4), dimethylnitrosamine (DMN), thioacetamide (TAA) and bile duct ligation (BDL)-induced rats. CCl4, DMN and TAA were administered intraperitoneally and conducted bile duct ligation for 4 weeks to induce hepatic fibrosis. Indices of liver cell injury (steatosis, hydropic degeneration, bile duct hyperplasia, hemorrhage & hemosiderin deposition), the extent of liver fibrosis (fibrotic area) and the rate of regeneration (number of PCNA-positive cells) were investigated in each group. Liver tissues were stained with hematoxylin-eosin (HE), sirius red, prussian blue and immunostained with α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), proliferative cell nuclear antigen (PCNA), and quantified using a computerized image analysis system. Liver cell steatosis was significantly increased in CCl4 and TAA groups, and hydropic degeneration and bile duct hyperplasia were significantly increased in TAA and BDL groups when compared with that in normal control, respectively. Fibrosis area was significantly increased in all four groups, especially in CCl4 group. Correlation between α-SMA and TGF-β1 expressions in four groups was good. Hemorrhage area in liver parenchyma was significantly increased in DMN group only when compared with that in normal control, while hemosiderin deposition area was significantly increased in TAA and BDL groups as well as DMN group. The Number of PCNA-positive cells was significantly increased in all four groups, especially in TAA group. These results indicate that the duration and methods of hepatotoxic drug treatment are very important factors to make plans for animal experimentation on the induction of hepatic fibrogenesis in rats. .

자궁경부상피의 악성변화에서 비만세포가 간질세포의 α-Smooth Muscle Actin 발현에 미치는 영향

제갈승주,최영자,이광주,노종섭,Jekal, Seung-Joo,Choi, Young-Ja,Lee, Kwang-Joo,Roh, Jong-Sup 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

There is increasing evidence that stromal reaction in cancer has an important diagnostic and prognostic significance. The aim of our study is to analyze the relation between the increase in mast cell number and the expression CD34 and alpha-smooth muscle actin (${\alpha}$-SMA) in the stroma of cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC). We investigated a total of 29 CIN (1,2,3) and 21 SCC (microinvasive and invasive) specimens and compared the distribution of $CD34^+$ stromal cells, ${\alpha}-SMA^+$ cells, transforming growth factor-${\beta}1$ $(TGF-{\beta}1)^+$ cells, and the density of mast cells using immunohistochemistry with antibodies against CD34, ${\alpha}$-SMA, TGF-${\beta}1$, and c-Kit (CD117) respectively. Computerized image analysis was to evaluate the positive area (%) and density of the respective immunoreactive cells. In CIN $CD34^+$ cells were abundant in the stroma but no ${\alpha}-SMA^+$ cells were identified except the wall of blood vessels. $CD34^+$ cells were progressively decreased along the continuum from CIN 2 to microinvasive SCC and not observed in the stroma of invasive SCC. Whereas ${\alpha}-SMA^+$ cells were only observed in the stroma of microinvasive and invasive SCC. We found more intense TGF-${\beta}1$ expression in the increased mast cells in the stroma of invasive SCCs than that in the stroma of CIN. These results indicate that disappearance of $CD34^+$ stromal cells and appearance of ${\alpha}-SMA^+$ cells are associated with the stromal change of CIN to SCC and the transformation of $CD34^+$ stromal cells into ${\alpha}-SMA^+$ cells is mediated by TGF-${\beta}1$ secretions in the stromal mast cell of SCC.

일밤 파라핀 절편에서 wet autoclave 전처리 후 AgNORs의 검출

제갈승주,곽효일,노종섭,이형섭 대한임상검사과학회 1997 대한임상검사과학회지(KJCLS) Vol.29 No.1

In the last few years the AgNORs technique has been used in tumor pathology for both diagnostic and prognostic patposes, but the lack of a standardizied staining protocol has frequently let to misinterpretation of actual structures evaluated by the various authors. The aim of the present study set out to measure the effect of wet autoclave pretreatment of routinely form alin -fixed and paraffin -embedded tissues on AgNORs staining. AgNOR quantity was evaluated n archival tumor tissues of 5 invasive adenocarcinoma of stomach , 8 invasive ductal carcinoma of breast and 14 invasive squamous cell carcinoma of uterine cervix. The treatment of sections at superheatedsteam temperature(121 degrees C) before silver incubation leads to a significantly better discrimination of individu a1 AgNORs , especially in AgNOR aggregates of nucleolus.Counting "black dots" by eye revealed considerably higher AgNOR mean numbers pernucleus in all pretreated cases when compared with the respective untreated controls. Also, mean AgNOR numbers increased after wet autoclave pretreatment in a linear manner(pairwise comparison by means of Pearson coπelation coefficient: r=0.89 instomach cancer, r=0.86 in breast cancer, r=0.86 in cervix cancer). It is suggested that the wet autoclave-pretreated method in routinely formalin-fixed and paraffin-embedded archival materials is more sensitive in detecting NORs than the untreated AgNORs staining and may prove a useful altemative for application in tumor pathology. .

Cyclooxygenase- 2 과발현은 CCl-induced 간섬유증의 증가된 mast cells와 관련이 있다.

제갈승주,이재형,박승택 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.4

Cyclooxygenase(COX-2) is an inducible enzyme that catalyzes the synthesis of prostaglandins (PGs) from arachidonic acid. Over-expression of COX-2 has been reported to be associated with progressive hepatic fibrosis in chronic hepatic C infection and rat liver fibrosis induced by carbon tetrachloride(CCl4). Recently, it is well known that mast cell products can stimulate the proliferation of hepatic stellate cells and key players in liver fibrosis. But little is known regarding their role in CCl4-induced liver fibrosis in rat. Our aim was to investigate the relation between COX-2 expression and mast cells during liver fibrosis after CCl4 treatment. Thirty Wistar rats were divided into five groups (non-treated 0, 2, 4, 6 and 8-week after CCl4-treatment). Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to assess the expression of α-smooth muscle actin (α-SMA), collagen-1 and COX-2 in liver tissue from CCl4-treated rats. The density of collagen and mast cells were determined using a computerized image analysis system in liver sections stained with picrosirius red and toluidine blue, respectively. The expression levels of α-SMA, collagen-1 and COX-2 mRNA were significantly higher at 2 wk in CCl4-treated groups than non-treated group. The number of mast cells in liver tissues increased gradually from 2 wk to 6 wk depending on the fibrosis severity but decreased abruptly at 8 wk. The significant increase of collagen-1 and α-SMA mRNA expression in CCl4-treated rats was continued until 6 wk while the COX-2 mRNA was significantly decreased at 8 wk. These results suggest that increased mast cells are closely associated with COX-2 over-expression during hepatic fibrogenesis of CCl4-treated rats. .

여러 염색조건이 AgNORs염색에 미치는 영향

제갈승주 대한임상병리사협회 1993 대한임상병리사회지 Vol.25 No.1

담관 결찰에 의한 간섬유증 발생에서 비만세포 동원에 미치는 Stem Cell Factor의 역할

제갈승주,( Grant A. Ramm ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.2

Mast cells (MCs) have been implicated in the pathogenesis of tissue fibrosis. However, the role of MC in the development of liver fibrosis has not been fully elucidated. Stem cell factor (SCF) is known to recruit MCs to the liver following injury as it induces mast cell proliferation, survival and differentiation from resident tissue precursors. This study examines the interaction between activated hepatic stellate cells (HSCs) and MCs in rat fibrotic liver, and SCF production by HSCs during culture in vitro. Rats were studied 4, 7, 14 and 21 days after bile duct ligation (BDL). Fibrogenesis was assessed by a measurement of collagen stained with sirius red F3B. Activated HSCs and MCs were identified by α-smooth muscle actin (α-SMA) immunohistochemical and alcian blue staining and measured by a computerized image analysis system. SCF production was determined in rat HSC cultures using Western blotting. Mild fibrotic changes were noted in BDL rat livers as early as 4 days after induction of cholestasis. Significant expansion and organization of fibrous tissue has occurred in day 14 BDL rats which progressed to bridging fibrosis by day 21. In BDL rats, both a large number of activated HSCs and MCs were detected in portal tracts and fibrous septa. Both area of activated HSCs infiltration and density of MCs were significantly higher in all BDL group compared with Shams. In BDL rats, both areas of activated HSCs infiltration and density of MCs were no significant difference between day 4 and 7 and were significantly higher in day 14. However, the areas of activated HSCs infiltration were significantly lesser in day 21 and the densities of MCs were significantly higher in day 21 compared with day14 BDL. In BDL rats, both areas of activated HSCs infiltration and density of MCs were highly correlated with areas of fibrosis. Western blotting showed that SCF protein was consistently produced in activated HSCs by culture on plastic and freshly isolated HSCs expressed relatively little 30kD SCF compared to late primary culture activated HSCs (day 14) and passaged HSCs. These results suggest that HSCs activated in vitro produce SCF, and may play an important role in recruiting mast cells to the liver during injury and fibrosis.

위선암에서 장형화생의 변이형에 관한 연구

제갈승주,최영자,곽효일,노정섭,최찬 대한임상병리사협회 2001 대한임상병리사회지 Vol.21 No.1

Helicobacter pylori 위염에서의 비만세포와 염증반응과의 관련성

제갈승주 ( Seung Joo Jakal ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Helicobacter pylori(H. pylori) induces severe inflammation and plays a key role in gastric mucosal disease. In general, mast cells are well known as initiators and regu1ators of inflammation, but their role in the gastric mucosal inflammation caused by H. pylori still remains unclear. πlerefore, this study was performed to investigate the differences of number of mast cell and mast cell degranulation in gastritis with and without H. pylori infection. Endoscopic biopsy specimens from 21 H. pylori-positive and 19 H. pylori-negative subjects were examined. The sections were cut from routine1y forma1in-fixed and paraffm embedded tissues, and stained with hematoxylin-eosin(HE), Warthin-Starry(WS) and 0.5% toluidine blue(TB) respective1y. HE-and WS-stained sections were assessed for inflammation and infection, and TB-stained sections were used in mast cell counting. The mast cells were quantificated the numbers per square millimeter using a computerized image analysis system. Mean number of mast cell and degranulated mast cell were significant1y higher in the mucosa with H. pylori infection than in the mucosa of noninfected gasπitis subjects. πlese also correlated significant1y with the inflammatory intensity Thus, mast cells may be important effector cells in the pathogenesis of H. pylori infected gastritis.