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마우스 모델을 이용한 상백피 추출물의 알러지성 천식 억제 효과
임홍진,김정근,심은기,홍철희,황충연 한국전통의학연구소 2004 한국전통의학지 Vol.14 No.1
The inhibitory effects of water extract of Cortex Mori(CM) was investigated for allergic asthma in a mouse model. Experimental allergic asthma was induced by repeated intraperitoneal sensitization and aerosol challenge of ovalbumin (OVA). CM extract (10mg/mouse/day) was administrated orally whereas control mice were given with identical volume of phosphate-buffered saline (PBS) for 7 days during the course of challenge. When airway hyperreactivity(AHR) measured by β-methacoline-induced airflow obstruction was compared, AHR of CM-treated mice was significantly lower than those of control mice, indicating that CM extract can attenuate an asthmatic symptom. Airway recruitment of leukocytes and cytokine levels in broncho-alveolar lavage fluid (BALF) was analyzed in order to evaluate CM effect on pulmonary inflammation. Total number of leukocytes in BALF was lower in CM-treated mice than in control without significance. Interestingly, CM treatment elevated a distribution of eosinophils which are crucial in asthmatic response. However, comparison of absolute number showed that it resulted from a decrease of total leukocyte and macrophage numbers. Levels of type 2 (Th2) cytokines (IL4, IL5 and IL 13) measured by ELISA in BALF were not significantly reduced by CM and IFN-γ, a type 1 (Th1) cytokine, was also comparable between two groups, indicating that CM treatment has little or no effect on airway inflammation and secretion of relevant cytokines. For an insight into the mechanism of CM effect on AHR, immunemodulatory activity of CM was analyzed at a cellular level. Peribronchial lymph node(LN) cells and lung-infiltrating lymphocytes (LIL) collected from CM-treated and control mice were in vitro stimulated with OVA antigen. Although there was no significance, number of LN cell and LIL was higher in CM-treated mice than controls and their Ag-specific proliferative response (3H-TdR uptake assay) was similar. Production of Th2 cytokine, IL 5 and IL 13 was relatively enhanced, but without statistical significance, while IFN-γ production was minimally altered in CM-treated mice. Immunemodulation of CM in the field of humoral immunity, antibody levels in serum were measured and compared. No significant difference was observed in the levels of IgE. However, levels of type 1 and 2 antibody IgG2a and IgG1 were signifcantly enhanced by CM treatment. As CM-mediated alleviation of AHR was not clearly explained by above results, degranulation of eosinophils was finally examined by their release of peroxidase. Levels of peroxidase in BALF was significantly lower in CM treated mice, suggesting that CM inhibits in vivo degranulation of eosinophils, although its effect was not shown during their in vitro incubation. Taken together, data from current study indicate CM extract alleviates AHR through inhibition of eosinophil degranulation rather than any immunological regulation. Instead, immunemodulatory activity of CM is likely to be adversely effective in (Th2)
Behc¸et의 患者의 單球細胞에서 溫淸飮의 cytokine 分泌抑制 효과
임홍진,황충연,강학천,김남권,권일호 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.4
Behc¸ets disease is a systemic inflammatory disorder. The etiology and pathogenesis of Behc¸ets disease has yet been fully elucidated but might involve immune dysfunction. Cytokines involved in the regulation of inflammatory reactions and immune responses may play a role in the pathogenesis of Behc¸ets disease (BD). Onchungeum is an Oriental herbal medication, which has been successfully used in Korea for the treatment of BD. This report describes modulation effects of Onchungeum on cytokine production from phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) of Behc¸ets patients by ELISA. Onchungeum significantly inhibited the production of pro-inflammatory cytokines, TNF-α and IL-1β, compared to absence of Onchungeum (by 52.3 1.4% inhibition for TNF-α and 113.5 3.3% for IL-1β, p < 0.001). Onchungeum also inhibited the production of IFN-γ, immunoregulatory Th1 cytokine, by 89.4 0.8% (p < 0.001). The inhibitory effects of Onchungeum on cytokine production showed dose-dependent manner, and the pre-treatment of 1㎎/㎖ Onchungeum had better effects than immunosuppressive drug for treatment of BD, cyclosporin A. Our results suggest that Onchungeum treatment for Behc¸ets disease patients may have pharmacologic activities and abilities of regulation of immune and inflammatory responses by cytokine modulation.