한방 안이비인후과학 수업에 적용한 새로운 패러다임의 교수법

임규상,이장천,박영규,Lim, Kyu-Sang,Lee, Jang-Cheon,Park, Young-Gue 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.1

Objectives : The purpose of this study is to survey the needs of Oriental Medical students about the existing teaching methods and to investigate satisfaction of new teaching & learning method on the Oriental medical Ophthalmology & Otolaryngology Class. Methods : 1. The needs of Oriental medicine whole students(475 persons) were surveyed with questioning paper. 2. Oriental medical Ophthalmology & Otolaryngology were studied by new paradigm teaching method with 116 students(3rd grade, school of Oriental medicine). 3. The satisfaction of school lesson of Oriental medicine 3rd grade students(106 persons) were surveyed with questioning paper before & after applying new paradigm teaching method. Results : 1. 43% students did not know well the purpose of lessons. 2. 46.7% students answered the curriculum has a large quantity. Conclusions : 1. Students want to study with various teaching & learning methods on school lessons. 2. 72.6% students were replied the problem based learning is effective to make a diagnosis and treatment of patients in the future. 3. 58.5% students take a part positively in the conduct of Team based learning study. 4. Team based learning was effective to the interaction with professor and students.

Neuropathic pain을 일으킨 쥐에 있어서 침의 진통작용

임규상,Lim, Kyu-sang 대한한방안이비인후피부과학회 1999 한방안이비인후피부과학회지 Vol.12 No.1

Background: The vasoreactivity of cerebral artery is currently the subject of increasing interest Transcranial Doppler The purpose of this study was to investigate the analgesic effect of acupuncture in a model of neuropathic pain produced by segmental spinal nerve injury (SSI) in rats. The left L5 and L6 spinal nerves of Sprague-Dawley rats were tightly ligated, and one week later, manual or electro-acupuncture was applied for 30 minutes to the contralateral hindlimb (right side) while the animal was lightly anesthetized with halothane. The mechanical threshold of the paw for ipsilateral (left side) hind limb flinching was determined prior to and 0.5, 1, 2, 4, and 24 hours after termination of acupuncture. The mechanical threshold for flinching was significantly elevated for about 4 hours after manual acupuncture applied to the BL. 60 (Kunlun) point or electro-acupuncture to the BL. 60 and BL. 40 (Weizhong) points. However, manual acupuncture applied to the ST. 36 (Zusanli) point was not effective. Furthermore, systemic naloxone pretreatment had no effect on the acupuncture induced threshold elevation. These data suggests that acupuncture produces a point specific, naloxone independent analgesia in a rat neuropathic pain model. ※ This paper is supported by a grant from the '1996 Jusan Foundation of Wonkwang university.)

학생 중심의 학습법을 적용한 한방 안이비인후과학 수업의 성과

임규상,이장천,박영규,Lim, Kyu-Sang,Lee, Jang-Cheon,Park, Young-Gue 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.2

Objectives : The purpose of this study is to survey the needs of oriental medical students about the existing teaching methods and to investigate satisfaction of student-oriented teaching & learning method on the oriental medical ophthalmology & otolaryngology class. Methods : 1. Oriental medical ophthalmology & otolaryngology were studied by student-oriented teaching method with 36 persons( 4th grade, school of Oriental medicine). 2. The satisfaction of school lesson was surveyed with questioning paper before & after applying student-oriented teaching & learning method. Results : 1. 88.9% students replied that the general teaching method is a lecture at school of oriental medicine. 2. They replied that Problem based learning(36.1%) & Team based learning(22.2%) are more effective teaching methods than lecture(19.4)%). 3. 66.6% students replied about the necessity of improvement of major subject's teaching method. Conclusions : 1. The interest & understanding degree were improved compare with other class by lecture. 2. It was positive about the interaction with professor and students(64.8%). 3. It was positive about the diagnosis and treatment of patients in the future(64.7%). 4. It was negative about the present national examination(67.6%). 5. Meditation was helpful at classwork(64.7%).

Classifying Host Susceptibility Using Porcine Circovirus Type 2 Viral Load and Antibody Titer

Kyu-Sang Lim(임규상),Eun-A Lee(이은아),Kyung-Tai Lee(이경태),Taehoon Chun(전태훈),Ki-Chang Hong(홍기창),Jun-Mo Kim(김준모) 한국생명과학회 2017 생명과학회지 Vol.27 No.3

양돈산업에 있어 돼지 써코바이러스 2형(PCV2)의 복합감염으로 인한 이유자돈의 질병 피해가 막대하다. PCV2 감염에 대한 숙주의 민감도는 상이한 것으로 알려져 있으며, 따라서 숙주의 민감도를 구분하는 것은, 이를 이용한 숙주의 저항성 향상 연구에 필수이다. 본 연구의 목적은 이유자돈군의 혈액 내에서 PCV2 바이러스에 대한 숙주의 민감도를 구분 짓고 구명하는데 있었다. 본 연구에서는 자연적으로 바이러스에 감염된 10주령의 이유자돈군으로 부터 혈청을 채취하여 PCV2 바이러스량과 항체가를 측정하고 혈구분석을 실시했다. 또한, 측정된 PCV2 바이러스량과 항체가를 기준으로 자돈군 내에서 저항성군과 민감성군을 선정하였고, 통계분석결과 저항성군에 비해 민감성군에서 백혈구 수가 현저히 줄어든 것을 확인하였다. 본 연구를 통해서 PCV2 감염에 대한 돼지의 민감도를 구분짓기 위한 PCV2 바이러스량과 항체가를 이용한 복합기준을 제시할 수 있었으며, 이유자돈군의 PCV2 관련 질병저항성 및 백혈구감소증을 확인할 수 있는 방법을 마련하였다. Porcine circovirus type 2 (PCV2) is a notorious and ubiquitous virus in the swine industry. The susceptibility of the host to PCV2 infection is considered to be one factor associated with the dynamics of PCV2. The objective of this study was to verify the criteria for host susceptibility to PCV2, using blood parameters of post-weaned pigs naturally infected with the virus. The PCV2 DNA viral load, antibody titer, and leukopenia characteristics were measured in the serum extracted from the pigs at the 10th week. We classified the pigs into high (>5.0), intermediate (3.0 to 5.0), and low (<3.0) groups on the basis of the PCV2 viral load (log copies/ml), or as positive (≤0.50) and negative (>0.50) groups on the basis of antibody titer (sample-to-negative corrected ratio). Moreover, using these two categorized parameters, we suggested the criteria for classification into the susceptible and resistant groups. Statistical analyses revealed that pigs in the susceptible group had a significantly higher viral load (p<0.001) and negative antibody titer (p<0.001), as well as significantly lower leukocyte counts (p=0.018) and lower amounts of several leukocyte components (p<0.05), than pigs in the resistant group. We concluded that the susceptible group could be considered to have PCV2-induced leukopenia. Therefore, we suggest that the combined classifications of viral loads and anti-PCV2 antibodies can be used to determine PCV2-induced leukopenia in the subclinical PCV2 infection of post-weaned pig populations.

The changes of goals and strategies in pig breeding area

Kyu-Sang Lim(임규상),Ki-Chang Hong(홍기창) 고려대학교 생명자원연구소 2015 생명자원연구 Vol.23 No.1

백서의 두 종류 관절염 모델에서 통증에 대한 전침의 진통 효과

문상돈,임규상,Moon, Sang-don,Lim, Kyu-sang 大韓外官科學會 2003 大韓外官科學會誌 Vol. No.

Although the use of acupuncture for pain has increased in recent years, the mechanisms of acupuncture analgesia (AA) remains obscure. The lack of suitable experimental animal models for persistent pain, which show clear AA, has been the major stumbling block in the investigation of the physiological mechanisms of AA. In the present study, we test AA in two knee arthritis model induced by injection of CFA or carrageenan as a chronic pain model. After induction of arthritis, the rat subsequently showed a reduced stepping force of the affected limb for the next several days. Electroacupuncture (EA) was applied to an acupuncture point each on the contralateral forelimb for 30 minutes under enflurane anesthesia. After the termination of EA, behavioral tests measuring stepping force were periodically conducted during the next several hours. EA produced a significant improvement of stepping force of the foot lasting for at least 2 hours when applied to LR2 In CFA model, and applied to ST36 in carrageenan model, but both points did not produce any significant effects in each other model. These data suggest that EA produce analgesic effect in knee arthritic pain and the analgesic effect is specific to the acupuncture point.

촉초(蜀椒)(천초(川椒)) 약대(藥對)에 관한 연구

이부균,임규상,강휘중,이장천,Lee, Boo-Kyun,Lim, Kyu-Sang,Kang, Hwi-Joong,Lee, Jang-Cheon 대한한의학방제학회 2012 大韓韓醫學方劑學會誌 Vol.20 No.1

Objectives : The purpose of this study is to find out herb-pair prototype composed of Zanthoxyli Fructus, to examine compatibility regulation between them and to classify them into seven features of compatibility. Methods : To search usages of Zanthoxyli Fructus compatibility, we have investigated the classics of herb and prescription such as "五十二病方"(Fifty-two kinds of Diseases), "黃帝內經"(The Medical Classic of Yellow Emperor), "傷寒論"(Treatise on Febrile Caused by Cold), "金?要略"(Synopsis of Prescriptions of The Golden), "附廣?后方"(Handbook of Prescriptions for Emergencies), "本草綱目"(Compendium of Materia Medica), "得配本草"(Deukbae Materia Medica). Results : In these books Zanthoxyli Fructus was paired with below herbs; 桂(Cinnamomi Cortex), 薑(Zingiberis Rhizoma), 吳茱萸(Evodiae Fructus), 細辛(Asari Radix), 茴香(Foeniculi Fructus), 肉荳?(Cistanches Herba), 釜下土(Terba Flava Usta), 益智仁(Alpinae Oxyphyllae Fructus), 飴(Saccharum Granorum), ?白(Allii Radix), 梨(Pyrus), 牙?(Gleditsia Fructus), 杏仁(Armeniacae Amarum Semen), 蕎麥粉(Fagopyrum esculentum Moench), 胡粉(Minium), 白朮(Atractylodis Rhizoma White), 蒼朮(Atractylodis Rhizoma), 麻子仁(Cannabis Fructus), 大麥蘗(Hordei Fructus Germiniatus), 麴(Massa Medicata Fermentata), 生地黃(Rehmanniae Radix), 茯?(Poria), 牡?(Ostreae Concha), 白礬(Alumen), 雄黃(Realgar), 烏頭(Aconiti Radix), 附子(Aconiti Lateralis Preparata Radix). Conclusions : Zanthoxyli Fructus herb-pair classified by seven features of compatibility are as follows; 1. Mutual reinforcement compatibility with : Cinnamomi Cortex, Zingiberis Rhizoma, Evodiae Fructus, Asari Radix, Foeniculi Fructus, Cistanches Herba, Terba Flava Usta, Alpinae Oxyphyllae Fructus, Saccharum Granorum, Allii Radix, Pyrus, Gleditsia Fructus. 2. Mutual assistance compatibility with : Armeniacae Amarum Semen, Fagopyrum esculentum Moench, Minium, Atractylodis Rhizoma White, Atractylodis Rhizoma, Cannabis Fructus, Hordei Fructus Germiniatus, Massa Medicata Fermentata, Rehmanniae Radix, Poria, Ostreae Concha, Alumen. 3. Mutual restraint compatibility with: Realgar, Aconiti Radix, Aconiti Lateralis Preparata Radix) 4. Mutual detoxication compatibility with: 桑汁(Juice of Mori Folium), 桑根汁(Juice of Morus Cortex), 大蒜(Allii Bulbus), 薺?(Remotiflori Radix), 桂汁(Juice of Cinnamomi Cortex).

Pine Oil이 白鼠의 血中 脂質代謝 改善 및 體重變化에 미치는 效果

김재주,임규상,유영수,Kim, Jae-ju,Lim, Kyu-sang,Lyu, Yeoung-su 대한한방안이비인후피부과학회 2000 한방안이비인후피부과학회지 Vol.13 No.1

The present study was carried out to investigate the effect of pine oil on the body weight and lipid levels of serum in rats fed high cholesterol diet and high fat diet, Body weight, weight of various organs, and feeding efficiency ratio were measured to study the effect of pine oil on obesty at 4 weeks after an oral administration, Total cholesterol, triglyceride, total lipid, HDL-cholesterol and LDL-cholesterol were also analysed to identify the ameliorating effect of pine oil on lipid metabolism in serum of same rats, The results were summerized as follows; 1. The increase in body weight and feeding efficiency ratio induced by choleserol diet was less in pine oil treated rats, Furthermore, decrease in weight of liver, kidney, spleen, testis, and epididymis were observed in pine oil treated rats. 2, Associated with the decrease in body weight, there was a concomitant reduction in serum levels of total cholesterol, triglyceride, and total lipid in rats fed high cholesterol diet and high fat diet. respectively, after an oral administration of pine oil. 3. Serum levels of LDL-cholesterol was significantly decrease after an oral administration of pine oil in rats fed high fat diet. These results suggest that pine oil can ameliorate obesity and lipid metabolism in serum.

오미자(五味子)의 멜라닌 생성 억제와 미백효과에 관한 연구

두인선,임규상,황충연,박민철,김남권,Doo, In-Sun,Lim, Kyu-Sang,Hwang, Chung-Yeon,Park, Min-Cheol,Kim, Nam-Kwen 대한한방안이비인후피부과학회 2007 한방안이비인후피부과학회지 Vol.20 No.3

Objective : The aim of this study is to assess the effect of Schizandrae Fructus on melanin synthesis inhibition and whitening effect. Methods : We assessed inhibitory effects of Schizandrae Fructus on melanin-release from B16F10, on melanin production in B16F10, on mushroom tyrosinase activity in vitro, on tyrosinase activity in B16F10 and effect of Schizandrae Fructus on the expression tyrosinase, TRP-1, TRP-2, PKA, ERK-1 ERK-2, AKT-1, MITF in B16F10. Results and Conclusion : 1. Schizandrae Fructus inhibited melanin-release, melanin production in B16F10. 2. Schizandrae Fructus inhibited tyrosinase activity in vitro and in B16F10. 3. Schizandrae Fructus suppressed the expression of tyrosinase, TRP-1, TRP-2, PKA, ERK-2 in B16F10.

흰쥐의 신경병증성(神經病症性) 통증(痛症) 모델에서 양로(養老) 자침(刺鍼)의 진통효과(鎭痛效果)

구성태 ( Sung Tae Koo ),양윤정 ( Yoon Jung Yang ),김산 ( San Kim ),유인식 ( In Sik Yoo ),임규상 ( Kyu Sang Lim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.3

Objectives: The usage of acupuncture has gained popularity for certain chronic pain conditions. However, the efficacy of acupuncture in various diseases has not been fully established and the underlying mechanism is not clearly understood. In the present study, the effect of electroacupuncture(EA) applied to yangno(SI6) on the neuropathic pain was examined. Methods: A common source of persistent pain in human is a neuropathic pain. Neuropathic pain was induced by tight ligation of L5 spinal nerve. When rats developed pain behaviors, EA was applied for 30 min. under enflurane anesthesia with repeated train stimuli at the intensity of 10X of muscle twitch threshold. The foot withdraw latency of the hind limb was measured for an indicator of pain level after each manipulation. Results: EA increased the mechanical threshold of the foot in the rat model of neuropathic pain significantly for the duration of 1 hr. suggesting a partial alleviation of pain. EA applied to SI6 point produced a significant improvement of mechanical sensitivity of the foot lasting for at least 1 h. However, ST36 point did not produce any significant increase of mechanical sensitivity. The improvement of mechanical threshold was interpreted as an analgesic effect. The analgesic effect was specific to the acupuncture point since the analgesic effect on the neuropathic pain model could not be mimicked by BA applied to a point, ST36. In addition, this analgesic effect of EA is mediated by a adrenergic mechanism of descending control of spinal cord from the brain. Conclusions: The data suggest that EA produces a potent analgesic effect on the neuropathic pain model in the rat; and 2) that EA-induced analgesia is mediated by a adrenergic mechanism of descending control in a point specific manner.