호장근으로부터 분리된 emodin의 혈관신생 억제 활성

이태규,김종화,소준노,Lee, Tae-Kyoo,Kim, Jong-Hwa,So, June-No 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1

호장근에서 분리한 emodin은 VEGF로 유도된 혈관신생의 한 단계인 혈관내피세포의 이동을 강하게 억제하였다. 또한 emodin은 혈관내피세포 이동을 억제시킨 $0.1\;{\mu}g/ml$ 농도에서 시험관내 혈관신생을 억제하였으며, 그 효과는 농도의존적인 양상을 보였다. 생체내 혈관신생 모델인 CAM assay에서도 emodin은 혈관신생을 억제하였다. 이러한 결과는 emodin이 현재까지 보고된 여러 가지 생리활성 이외에 혈관신생 억제활성을 가지고 있다는 사실을 보여주는 것이다. 따라서 emodin을 함유하고 있는 호장근은 혈관신생 관련 질환에 대한 천연물 유래 치료제의 개발을 위한 중요한 식물자원으로 활용될 수 있을 것이다. Polygonum cuspidatum has been used as a fork medicine for a long time. Emodin was purified from the root of P. cuspidatum by thin layer chromatography (TLC) and preparative high perfomance liquid chromatography (HPLC). The effects of emodin on the migration of endothelial cells and in vitro angiogenesis stimulated with vascular endothelial cell growth factor (VEGF) were examined, using human umbilical vein endothelial cells (HUVECs) and porcine pulmonary arterial endothelial cells (PPAECs). Emodin potently inhibited the VEGF-induced migration of (HUVECs) at relatively low cocentrations $(0.1-10\;{\mu}g/ml)$; the inhibition of endothelial cells by emodin was 75.4% at $0.1\;{\mu}g/ml$ and about 90% at $1\;{\mu}g/ml$. Emodin also inhibited VEGF-induced sprout formation in vitro at concentrations of $0.1-10\;{\mu}g/ml$. Emodin was also evaluated for the inhibitory potential on in vivo angiogenesis in a growing chick embryo chorioallantoic membranes (CAM). At a concentration of $1.0\;{\mu}g/ml$ Per disc, emodin was able to induce avacular zone in the CAMs. These findings suggest that emodin is a potent angiogenesis inhibitor and P. cuspidatum is a useful herb in the development of therapeutics for angiogenesis dependent diseases.

호장근으로부터 분리된 emodin의 혈관신생 억제 활성

이태규 ( Tae Kyoo Lee ),김종화 ( Jong Hwa Kim ),소준노 ( June No So ) 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

Po1ygonum cuspidatum has been used as a fork medicine for a long time. Emodin was purified from the root of P. cuspidatum by thin layer chromatography (TLC) and preparative high performance liquid chromatography (HPLC). The effects of emodin on the migration of endothelial cells and in vitro angiogenesis stimulated with vascular endothelial cell growth factor (VEGF) were examined, using human umbilical vein endothelial cells (HUVECs) and porcine pulmonary arterial endothelial cells (PPAECs). Emodin potently inhibited the VEGF-induced migration of HUVECs at relatively low cocentrations (0.1 -10㎍/㎖); the inhibition of endothelial cells by emodin was 75.4% at 0.1 ㎍/㎖ and about 90 % at 1 ㎍/㎖. Emodin also inhibited VEGF-induced sprout formation in vitro at concentrations of 0.1 -10㎍/㎖. Emodin was also evaluated for the inhibitory potential on in vivo angiogenesis in a growing chick embryo chorioallantoic membranes (CAM). At a concentration of 1.0 ㎍ per disc, emodin was able to induce avacular zone in the CAMS. These findings suggest that emodin is a potent angiogenesis inhibitor and P. cuspidatum is a useful herb in the development of therapeutics for angiogenesis dependent diseases.

능이 [Sarcodon aspratus (Berk.) S. Ito]중 蛋白質 加水分解 酵素의 精製 및 性質에 관하여

이태규(Tae-Kyoo Lee) 한국식품영양과학회 1986 한국식품영양과학회지 Vol.15 No.3

능이 [Sarcodon aspratus (Berk.) S. Ito]로부터 蛋白質加水分解 酵素를 분리 정제하여 그 특성을 조사하였다.<br/> 본 효소는 Tri-acryl CM-cellulose, Ultrogel AcA 54, Hydroxy apatite column chromatography와 preparative isoelectric focusing 방법으로 순차 정제되었고, 정제된 효소는 PAGE상에서 단일 band를 나타내었으며 活性은 62.5O, D/㎎, protein으로 粗酵素보다 8.01배 증가하였다.<br/> 作用最適 pH는 10.1로 alkaline protease였으며 作用最適 溫度는 57℃부근이었다.<br/> 50℃ 이하의 溫度와 pH 4.0~10.8 범위에서 安定하였으나, 60℃에서 30분후 26℃, 65℃에서는 65℃가 失活되었다.<br/> Mn^(++)은 효소의 活性을 증가시켰으나 Hg^(++)와 Cu^(++)는 현저히 저해시켰다.<br/> SDS-PAGE에 의해 分子量은 30,100으로 측정되었고, monomer임이 확인되었다.<br/> 等電點은 9.80이었다.<br/> 본 연구는 1985년 문교부 학술연구조성비에 의해 연구되었읍니다. This study was undertaken to investigate the characteristics of the proteolytic enzyme extracted from Neungee mushroom [Sarcodon aspratus (Berk.) S. Ito].<br/> The enzyme was purified by using Tris-acryl CM-cellulose ion exchange, gel filtration on Ultrogel AcA 54, Hydroxy apatite column chromatography and preparative isoelectic focusing.<br/> The specific activity of the purified enzyme increased 8 times as compared with that of the crude enzyme. The enzyme was homogeneous on polyacrylamide gel electrophoresis (PAGE).<br/> The optimum pH was 10.1, indicating the enzyme to be alkaline protease and the optimum temperature was 57℃.<br/> The enzyme was stable at temperatures lower than 50℃ and at pH values ranging from 4.0 to 10.8.<br/> However, the enzyme activity decreased by 26 and 65% at 60 and 65℃, respectively, when incubated for 30 minutes.<br/> The enzyme activity was activated by Mn^(++) and inhibited by Cu^(++) and Hg^(++).<br/> The enzyme was consisted of monomer and its molecular weight estimated to be about 30,100 when determined by sodium dodecyl sulfate PAGE.<br/> Isoelectric point of the enzyme was determined to be 9.80.

부패된 팥앙금으로부터 분리된 미생물의 동정과 그 균의 생리적 특성

이태규(Tae-Kyoo Lee),노민환(Min-Hwan Roh) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.10

In order to determine the causes of sweetened adzuki ann spoilage, the characteristics of microorganism isolated from spoiled adzuki ann were investigated. The isolated microorganism was gram-positive, rod-shaped and spore-forming bacteria; its surface was smooth and glazed. From the results of the assimilation test of 46 different biochemicals by the Vitec 2 Compact test and comparison of the cellular wall composition of fatty acid by the data bank of Midi sherlock system, the microorganism was identified as Bacillus subtilis. D-value of the B. subtilis spore was 4.85 min at 115℃, 0.69 min at 121℃ and 0.48 min at 125℃; Z-value was 9.71. The Bacillus subtilis growth was not observed below water activity of 0.92 at 45℃. However, bacteria growth increased gradually as water activity increased above 0.93.

Effect of Degumming Reagents on the Recovery and Nature of Acetone Insolubles from Rice Bran Oil

이태규(Tae-Kyoo Lee),노민환(Min-Whan Rho),양희천(Hee-Cheon Yang),김충기(Choong-Ki Kim),송근섭(Geun-Seoup Song),엄태붕(Tai-Boong Uhm),권용주(Yong-Ju Kwon) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.3

물 및 다섯가지 탈검제(citric acid, phosphoric acid, oxalic acid, acetic anhydride 및 maleic anhydride)를 사용하여 미강유를 탈검하고 그 탈검효율을 비교하였다. 미강유 원유로부터 인 함유 물질을 제거하는데 있어서 모든 탈검제가 보다 효과적이었으며, 특히 acetic anhydride와 phosphoric acid는 각각 92.5% 및 93.3%까지 인 함유율을 저하시켰다. 또한 비수화성 인지질 특히 Lyso PC를 제거하는데 있어서 모든 탈검제가 효과적이었다. 미강유로부터 추출한 AI의 인지질은 주로 PC, PI, PE 및 Lyso PC로 구성되어 있었으며, 주요 지방산은 oleic acid, linoleic acid 및 plamitic acid의 순이었다. Acetic anhydride를 사용하여 얻은 AI가 가장 안정된 유화를 형성하였으며, 반면에 phosphoric acid 및 oxalic acid를 처리하여 얻은 AI는 매우 빈약한 유화성을 나타냈다. Six reagents (water, citric acid, phosphoric acid, oxalic acid, acetic anhydride and maleic anhydride) were evaluated for their effectiveness in degumming rice bran oil. All chemical reagents tested were found to be significantly more effective than water in removing phosphatides from crude rice bran oil. Especially acetic anhydride and phosphoric acid were effective in reducing phosphorous levels (92.5% and 93.3% removal, respectively). Nonhydratable phospholipids, lysophosphatidyl choline, were removed more effectively by the chemical reagents than by the water degumming. The major phospholipid(PL) components were phosphatidyl choline. Oleic, linoleic and palmitic acids were the major fatty acids of PL in rice bran acetone insolubles(AI). The AI recovered by acetic anhydride degumming produced the most stable emulsions. However, the AI obtained from phosphoric acid or oxalic acid treatments had very poor emulsifying properties.

능이[Sarcodon aspratus (Berk.) S. Ito]중 알카리성 단백질가수분해효소의 1차구조에 관한 연구 - Ⅰ. 아미노산 조성, 활성부위 아미노산 및 N - 말단 부위의 아미노산 배열

이태규(Tae-Kyoo Lee) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.22 No.6

Sarcodon aspratus(Berk.) S. Ito에서 분리정제한 단백질가수분해효소의 특성을 조사하였다. 이 효소는 당을 2.1% 함유하고 있었다. Rose bengal, N-bromo succinimide, phenyl methyl sulfonyl fluoride(PMSF)와 같은 화학적 수식 시약에 효소 활성이 저해되었으며, 1차 반응속도론적 불활성 mode를 가지므로 활성 부위는 tryptophan과 serine으로 추정된다. N-말단에서 21번째 잔기까지의 아미노산 배열은 V-T-T-K-Q-T-N-A-P-W-G-L-G-N-I-S-T-T-N-K-L-으로 동정되었다. Properties of a protease purified from Sarcodon aspratus (Berk.) S. Ito have been investigated. The enzyme displays as a glycosylated serine protease. The sequence for the 21 amino acids of the N-terminal side in the enzyme was determined by automated sequence analysis. The sequence was V-T-T-K-Q-T-N-A-P-W-G-L-G-N-I-S-T-T-N-K-L-

중풍입원환자의 체질별 소증(素證)과 중풍 발생 후 병증(病證)에 관한 임상적 연구

함통일,황민우,이태규,김상복,이수경,고병희,송일병,Ham, Tong-Il,Hwang, Min-Woo,Lee, Tae-Kyoo,Kim, Sang-Bok,Lee, Soo-Kyung,Koh, Byung-Hee,Song, Il-Byung 사상체질의학회 2004 사상체질의학회지 Vol.16 No.3

1. Objectives The purpose of this study is to investigate constitutional ordinary and pathological symptoms differences among each constitutional groups of stroke in-patients. 2. Methods 101 stroke in-patients(Soyangin(SY) $n^{a)}=52$, Taeumin(TE) n=39, Soeumin(SE) n=10) hospitalized at Kyung-Hee Oriental medical center from Nov. 2003 to Sept. 2004 were investigated through questionnaire which consists of 18 parts, 289 questions(156 questions concerned ordinary symptoms and 133 pathological symptoms). The answers were analyzed statistically in order to find the questions which represent the significant differences among each constitutional groups. ( a) number of patients) 3. Results As for the ordinary symptoms, majority of the Soyangin group replied that they experienced "frequent dreams during sleep" and "scarce constipation". Majority of the Taeumin group affirmed to "dark redness of face", "dry nose", "low intolerance of heat", "good appetite and digestion", and "frequent overeating experiences". Majority of the Soeumin group affirmed to "pale face", "insomnia", "frequent constipation", "poor appetite and digestion", "small amount of diet", "slow eating", "fond of warm or hot water", and "easily tired(especially after sweating)". As for the pathological symptoms, majority of the Taeumin group affirmed to "frequent optic fatigue", "frequent tinnitus", "frequent bitter mouth", "fond of cold water", "flatulent", "stuffiness in the navel area", "irregular stool habits", "frequent constipation", "large amount of sweat", and "frequent rigidity of neck". Majority of the Soeumin group affirmed to "bad appetite", "fond of warm or hot water", "drink small amount of water", "sensitive during sleep" and "frequent heavy head". 4. Conclusions Through this study, We found statistically significant questions distinguishing the characteristics of each constitutional group.

고농도 대두가공폐수의 처리를 위한 개선 활성슬러지법

조권익,이정수,이태규,김종화,Cho, Kwon-Ik,Lee, Jeoung-Su,Lee, Tae-Kyoo,Kim, Jong-Hwa 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.1

활성슬러지에 의한 생물학적 폐수처리에 있어 주된 관리인자는 유기물의 효율적인 제거와 슬러지의 침강성을 일정치 이하로 유지하는 것이다. 식품폐수의 하나인 고농도의 대두가공폐수를 일반적인 활성슬러지법을 적용한 결과 최적 F/M비(food-to-microorganism ratio)는 0.24(kg-BOD/kg-MLVSS day)였으며 그 이상의 농도인 0.48(kg-BOD/kg-MLVSS day)에서는 슬러지 팽화현상이 발생하여 고농도의 식품폐수처리에는 효율적이지 못하였다. 이를 개선하기 위하여 응집보조제(NaOH)를 활용하여 유입폐수의 pH를 9.0으로 조절한 결과, 2.88(kg-BOD/kg-MLVSS day)의 고농도 폐수를 유입하여도 슬러지의 팽화현상없이 SVI(sludge volume index)를 150 이하로 유지하였다. 이것은 최대 허용부하를 일반적인 활성슬러지법에 비하여 7.2배 높일 수 있는 효율적인 방법으로 평가되었다. The kernel of wastewater treatment by activated sludge is elimination of organic substances and maintenance of well-flocculated sludge sedimentation. By the conventional activated sludge treatment, the optimum F/M ratio of soybean wastewater treatment was 0.24 (kg-BOD/kg-MLVSS day) and sludge bulking was generated at 0.48 (kg-BOD/kg-MLVSS day). To improve the treatment capacity and operation quality in higher loading of soybean wastewater, influent pH was constantly controlled by 9.0 using NaOH as a coagulant agent. In this process, higher loading up to 2.88 (kg-BOD/kg-MLVSS day) was possible and SVI was maintained under 150 without bulking. This was equivalent to 7.2 times higher than maximum permissible load of the conventional activated sludge process.

물엿농도와 열처리 조건에 따른 팥앙금 호화액의 당 및 아미노산의 변화 모니터링

노민환 ( Min Whan Rho ),이태규 ( Tae Kyoo Lee ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2006 한국식품저장유통학회지 Vol.13 No.5

다슬기 ( Semisulcospira libertina ) 의 영양학적 성분에 관한 연구

김용휘(Yong Hwi Kim),이태규(Tae Kyoo Lee),차연수(Yeun Soo Cha) 전북대학교 농업과학기술연구소 1985 농업생명과학연구 Vol.16 No.-