매독환자에서 19S ( IgM ) - FTA 검사소견

이정복(Jung Bock Lee),이민걸(Min Geol Lee),김형일(Hyung Il Kim),이성낙(Sung Nack Lee) 대한피부과학회 1985 대한피부과학회지 Vol.23 No.5

In 228 sera from untreated syphilitic patients with different clinical stages and 52 healthy persons, the presence of treponema-specific 19S(IgM) antibodies were demonstrated by using the l9S(IgM)-FTA test. Of 228 sera, 160 were syphilitic sera based on symptoms, clinical history and serologic tests (VDRL, FTA-ABS and TPHA). The sensitivity of the 19S(IgM)-FTA test was 98g in 160 syphilitic sera, especially lp0, in primary syphilis. Remaining 68 positive sera(30) in VDRL, FTA-ABS or TPHA showed nonreactive in ]9S(lgM)-FTA test, which seemed to be spontaneously healed sera. The specificity of the l9S(IgM)--FTA test was 98% in 52 healthy sera.

매독환자에서의 뇌척수액 검사소견

이정복(Jung Bock Lee),김수찬(Soo Chan Kim),이민걸(Min Geol Lee),이성낙(Sung Nack Lee) 대한피부과학회 1985 대한피부과학회지 Vol.23 No.3

The cerebrospinal fluid findings in 316 cases of untreated syphilis with different clinical stages and 92 cases of adequately treated syphilis examined at least 1 year after treatment with penicillin are resented. Examination of the CSF included VDRL, FTA-ABS cell count and total protein determination. Adequately treated cases showed lower reactivity of serologic tests of CSF compared with untreated cases. However there were no significant differences in cell counts and protein concentration between untreated and adequately treated cases. Thus we suggest that the presence of pleocytosis and raised protein concentrations in CSF are not taken as indications of active neurosyphilis.

1기 매독 병변을 공유한 2기 매독 3예

이민걸(Min Geol Lee),김형주(Hyung Joo Kim),이정복(Jung Bock Lee) 대한피부과학회 1986 대한피부과학회지 Vol.24 No.1

We present herein 3 cases of early secondary syphilis with simultaneous primary syphilitic lesions. These cases developed primary syphilitic lesions four to six weeks after sexual contact and lasted six to ten weeks thereafter until the secondary syphilitic lesions appeared. All three patients showed a strong positive for the STS. Spirochetes were seen under dark field examination of the primary syphilitic lesions, Histopathologically, numerous plasma cells around capillaries and endothelial hyperplasia were seen in the dermis.

탈모증 환자에서 Minoxidil 국소 도포제의 치료효과 - 공동연구 -

이민걸 ( Min Geol Lee ),윤문수 ( Moon Soo Yoon ),이정복 ( Jung Bock Lee ),노병인 ( Byung In Ro ),김형옥 ( Hyung Ok Kim ),정경재 ( Kyung Jae Chung ),허충림 ( Choong Rim Haw ),이규석 ( Kyu Suk Lee ),김수남 ( Soo Nam Kim ),손숙자 ( S 대한피부과학회 1986 대한피부과학회지 Vol.24 No.2

매독 환자의 병변에서 Avidin - Biotin - Peroxidase Complex법을 이용한 Treponema pallidum 검출에 관한 연구

이민걸(Min Geol Lee),이원수(Won Soo Lee),이정복(Jung Bock Lee) 대한피부과학회 1989 대한피부과학회지 Vol.27 No.1

Using the avidin-biotin-peroxidase complex(ABC) technique, 36 skin specimens from 30 patients with primary and secondary syphilis and a gastric mucosal specimen from a patient with secondary syphilis which were confirmed by clinical history, physical examination, the VDRL, FTA-ABS, TPHA, and 19s(IgM)-FTA test, were examined. The results were compared with that of the indirect mmunoperoxidase technique which was done by authors previously with same specimens. The following results were obtained. 1. Of the 37 specimens, 35(95%) were positive in ABC technique and 33 of the 37 specirnens(89%) were positive in the indirect immunoperoxidase technique. 2. The ratio of agreement of the results between the ABC and the indirect immunoperoxidase technique was 89%. 3. Most of the treponemes were located in the upper dermis, epidermis, and blood vessel walls in the arder named, and rarely in the lower dermis of the syphilitic skin lesions. There was no remarkable difference in histologic distribution of treponemes between the clinical stages and types of syphilitic skin lesions. :From the results, the ABC technique is considered to be an excellent method for detecting the treponemes in the suspected syphilitic lesions.

매독환자의 혈청 IgM 항체에 반응하는 매독균 단백항원에 관한 연구

이민걸(Min Geol Lee),성열오(Yeol Oh Sung),김동건(Dong Kun Kim),이정복(Jung Bock Lee) 대한피부과학회 1989 대한피부과학회지 Vol.27 No.4

This study was undertaken to identify the protein antigens reacting with IgM antibodies in the sera of various stages of syphilis before and after treatment, employing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and immunoperoxidase stain. The results were as follows : 1. Before treatment, the most strongly reacting antigens of T. pallidam precipitated by IgM antibodies in the sera of patients were polypeptides of molecular weights 47,000, 34,000 and 29,500. So it was observed that those were the major antigens of T. pallidum reacting with IgM antibodies. 2. After observing protein antigens of T. pallidum reacting with IgM antibodies in the sera of patients with syphilis before and after treatment, it was seen that in primary, secondary and early latent syphilis there was a loss of several antigens and s decrease in reactivity, but no changes occurred in late latent and reinfected syphilis. 3. From the observation of the reaction between serum antibodies of patients with trested syphilis and major antigens of T. pallidum, an evident decrease in reactivity was observed only with protein antigen of molecular weight 47,000 which reacts with IgM antibody. From the above results, it could be concluded that of molecular weight 47,000 could contribute to the assessment of the diagnosis of syphilis and the efficacy of treatment.

매독균의 분리 정제에 관한 비교연구

이민걸(Min Geol Lee),조성환(Sung Whan Cho),이광훈(Kwang Hoon Lee),이정복(Jung Bock Lee) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.1

There are several different methods of purifying Treponema pallidum(TP) from rabbit testicular tissue. Among them, we compared the use of differential centrifugation, which has been most widely used, to Percoll density gradient centrifugatian, a newly applied method, in purifying TP from rabbit testicular itissue by checking the protein concentration of the TP suspension, hemagglutination assay using sheep erythrocytes sensitized by TP, IgM-TP-enzyme-linked immunosorhent, assay(IgM-TP-ELISA) and eJect,ron microscopic observation. The protein concent,ration af TP antigen suspension (2x] OTV/ml) purified by Percoll density gradient centrifugation (lower band) was the lowest (129.0pg/ml) when compared t.o those purified by differential centrifugation (324.0pg/ml) and Percoll density gradient centrifugatian (upper band) (560.2pg/ml). Sheep erythrocytes sensitized by TP purified by Percoll density gradi- ent centrifugation(lower band) showed the same resiilts as those using a commercii1 TPHA kit when tested with positive and negative control sera. The sensitivity and specificity of the IgM-TP-ELISA were 88.5%(2'3/26') and 86.4%(19/2Z) respectively using TP as an antigen purified by differential centrifugation. The rates were 96.29!i (25/26) and 95.5%(2l/22) using TP purified by Percoll density gradient centrifugation. As shown by the electron microscopy, T. pizllida purified by clifferential centritugation and Percoll density gradient centrifugatiori were structurally unaltered, and Percoll-purified TP contained much less tissue debris than TP prepared by differ ential centrifugation. Therefor e, Percoll density gradient centrifugation is considered to be a better method of purifying TP from rabbit testicular tissue when compared to differential centrifugatian, as a matter of fact, Perrol1 density gradient centrifugation has been applieci successfully in the study of the physiology, recombinant DNA techniques, and antigenic structure of TP and to the preparation of the antigen for the FTA-ARS and TP-ELISA (Kor J Oermatol 29(1): 56 64, 99)

국내 정상 웅성 가토의 매독 혈청 검사 양성율

이민걸(Min Geol Lee),김형주(Hyung Joo Kim),한승경(Seung Kyung Hann),이정복(Jung Bock Lee) 대한피부과학회 1986 大韓皮膚科學會誌 Vol.24 No.5

The present study has been undertaken, using 307 seemingly normal male rabbits sold in Korea, which were tested for sysphilis with two serologic tests, the RPR card test and the TPHA test. Among thc rabbits which were seropositive in these tests, 10 rabbits were selected for further tests, namely, the FTA test a.nd FTA-ABS test. The following results have been obtained. Nine rabbits(2.9%) were reactive in both the RPR card test and the TPHA test; 10(3.2%) were reactive in the RPR card test and non-reactive in the TPHA test; 8(2. 6%) were reactive in the TPHA test and non-reactive in the RPR card test, and 27(8.7%) were reactive in either tests. 2. The rabbits which were reactive in the TPHA test were also reactive in the FTA and FTA-ABS test. As can be seen in the results, 8.7% of the normal male rabbits sold in Korea are seropositive in the serologic tests for syphilis. Cnnsequently, all rabbit,s undergoing syphilis experiments should be screened for possible reactive serologic tests and excluded from the experiments.

매독환자의 혈청내 면역복합체에 관한 연구

이민걸,곽호,박주영,김세종,이정복 ( Min Geol Lee,Ho Kwahck,Joo Young Park,Se Jong Kim,Jung Bock Lee ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

N/A Circulating immune Complex in Syphilis Min Geol Lee, M.D., Ho Kwahck, M.D., Joo Young Park, M.D.*, Se Jong Kim, M.D.*, Jung Bock Lee, M.D. Departments of Dermatology, Microbiology* Yonsei University College of Medicine, Seoul, Korea In this study, the author tried to clarify the relationships between the clinical stages of untreated syphilis and the positive rates of circalating immune complex(CIC). Additionally, the study looks at the duration of CIC conversion to negative in the treated previously positive patient by means of solid phase CIq enzyme assay(CIq EA), solid phase anti-CIq enzyme assay (anti-Ciq EA), and platelet aggregation test(PAT)to get the following results. The results are as follows : 1. Among the 132 untreated patients, 36 patients(27.3%) were CIC positive in at least one of the three tests performed, and in the negative groups, two out of 50(4.0%) were CIC positive. 2. The positive rates beginning with the highest were as follows : secondary syphilis, early latent syphilis, late latent syphilis, and primary syphilis. 3. Among the seven CIq EA positive patients before treatment, two had negative conversion in the first week after treatment. Among the remaining five, one remained positive after three weeks and four after three months. 4. Among the six anti-CIq EA positive patients before treatment, there were three negative conversions after two weeks, one after three months ; the remaining two stayed positive. 5. The one PAT positive patient showed negative coversion after one week of treatment.

매독균이 HaCaT세포의 표면 항원 발현에 미치는 영향

이종서 ( Jong Seo Lee ),이민걸 ( Min Geol Lee ),이광훈 ( Kwang Hoon Lee ),이정복 ( Jung Bock Lee ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5

Background: In primary syphilis, Treponema pallidum invades skin and mucosa through the epidermis, enters blood vessels and circulates in the blood. Afterwards, T. pallidum migrates out of the blood vessels to the pidermis in secondary syphilis to induce diverse skin findings. Therefore, the epidermis seems to be one of the main targets in syphilis. Most of the epidermal cells are keratinocytes and it ca i be presumed that, T. pallidum infection elicit,s defenr.e mechanisms at kerat.inocyte level resulting in changes of the keratinocyt,e surface molecule expression. Objective .' We have cxamined by ELISA whether virulent T. pallidum and its 47-kDa antigen could change the expre.;sions of E cadherin, MHC I, and ICAM 1 on the surface of HaCaT, a transformed human kerai.inocyte cell line. Methods . The 96 well plates of 4 x 10' HaCaT cells/well were cultured for 1 2 days at 37g, and cultured for 16 hoi!rs at 37g including 3x 10' T. pallidum per well or its 47-kDa antigen. Then the changes of E-cadherin, MHC I and, ICAM-I were observed by ELISA. Media alone was used for negative cont,ro: and TNF a of 10U/well was used for positive control. Results : On culturing with T. pallidum, the expression of E-cadherin and MHC I increased sig- nificantly compared with the negaiive control. That of ICAM 1 showed increased findings com- pared t,o the negative control, but, it was not, significant. On culturing with its 47 kDa antigen, the expression of E-cadioerin and MHC-I increased significantly compared with negative control. ICAM-1 expression was increased compared to the negative cont,rol, but it. was not significant,. Conclusion . The increase in E-cadherin and MHC I expressions in HaCaT cells cultured with T. pallidum may be related to the defence mechanisms of the host cells. (Kor J Dermatol 1996;34(5): 797-804)