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치주인대세포에서 Aggregatibacter actinomycetemcomitans의 IL-8 및 활성산소종 유도능
이양신,박홍규,김성환,차정헌,유윤정,Lee, Yang-Sin,Park, Hong-Gyu,Kim, Sung-Whan,Cha, Jeong-Heon,Yoo, Yun-Jung 대한치주과학회 2009 Journal of Periodontal & Implant Science Vol.39 No.3
Purpose: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of $O_2$. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. Methods: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. Results: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. Conclusions: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
조골세포에서 Aggregatibacter actinomycetemcomitans 생균의 파골세포분화유도 cytokine 발현 유도능 및 침투능
최호길,이양신,김민영,김경대,차정헌,유윤정,Choi, Ho-Kil,Lee, Yang-Sin,Kim, Min-Young,Kim, Kyoung-Dae,Cha, Jeong-Heon,Yoo, Yun-Jung 대한치주과학회 2007 Journal of Periodontal & Implant Science Vol.37 No.3
Osteoblasts regulate osteoclastogenesis by production of various cytokines. Aggregatibacter(A) ac-tinomycetemcomitans is one of periodontopathogens which invades gingival tissue. Therefore, clarifying the effect of alive A. actinomycetemcomitans on osteoblasts is important to understand the mechanism of alveolar bone resorption in periodontitis. We investigated induction of osteoclastogenesis-inducing cytokines, adherence, and invasion by A. actinomycetemcomitans in osteoblasts. Osteoblasts were isolated from mouse calvaria and expression of cytokines was determined by RT-PCR. When the ratio of the number of A. actinomycetemcomtians to the number of osteoblasts was 10:1, 50:1 and 100:1, RANKL mRNA expression was increased. A. actinomycetemcomitans also increased expression of macrophage inflammatory protein (MIP) -1${\alpha}$, interleukin (IL)-1${\beta}$, and tumor necrosis factor (TNF)-${\alpha}$. A. actinomycetemcomitans attached to and invaded osteoblasts at ratio of 1000:1. These results suggest that A. actinomycetemcomitans increases osteoclastogenesis-inducing ability of osteoblasts by stimulating the expression of RANKL, MIP-1${\alpha}$,IL-1${\beta}$, and TNF-${\alpha}$ and that invasion of A. actinomycetemcomitans provides a means by which the bacteria escape from immune system and antibiotic therapy.
조광현,황윤호,이양행,류지훈,최강주,곽기오,최석철,양영일,최영균,신치만 인제대학교 1999 仁濟醫學 Vol.20 No.1
97년 9월 이후 2예의 동소 심장이식수술을 시행하였는데 대상 환자들은 남녀 각 1명, 나이는 여자는 55세, 남자는 23세이고, 체표면적은 각각 1.54m2, 1.62m2였다. 대상환자들은 확장성 심근증과 제한성 심근증 환자로 NYHA IV 정도의 심한 심부전증 증상이 있었다. 수술 후 면역억제제로는 사이클로스포린, 아자티오프린, 스테로이드를 사용하였다. 첫 번째 환자에서는 수술 10일 후 심근조직검사에서 ISHLT 기준의 I 단계의 거부반응을 보였고, 이후의 조직검사에서는 거부반응이 없었으며 술후 2개월째에 대상포진, 8개월째에는 구강 칸디다증으로 입원 치료를 받았다. 두 번째 학자에서는 술후 2일째 사이클로스포린에 의한 신독증을 보였으나, 순조롭게 회복되어 퇴원하였다. Since September 1997, we have performed two cardiac transplantations in one dilated cardiomyopathy and one restrictive cardiomyopathy. The patients were a 55-year-old female and a 23-year-old male, and their body surface area(m2) were 1.51 and 1.62 respectively. Both patients were suffered from dyspnea as NYHA functional class IV. Postoperative immunosuppression was accomplished with cyclosporine, azathioprine and predinisolone. In case 1, there was an ISHLT grade IA rejection in endomyocardial biopsy at postoperative 9th day which disappeared at the tome of next biopsy 20 days later. The patients were treated for herpes zoster and oral candidiasis at postoperaive 2 months and 8 months, respectively. In case 2, nephrotoxicity related to cyclosporine developed 2 days after the surgery, but was soon resolved and the patient was discharged in good condition.