http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Inhibition of LFA-1/ICAM-1-mediated Cell Adhesion by Stilbene Derivatives from Rheum undulatum
이승웅,노문철,Byung Soon Hwang,Mi-Hwa Kim,박찬선,이우송,오현미 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.10
Six stilbenes were isolated from the methanol extract of Rheum undulatum rhizomes by bioactivity-guided fractionation. The structures of the compounds were determined by spectroscopic analysis (1H-, 13C-NMR and MS), to be desoxyrhapontigenin (1), rhapontigenin (2), trans-resveratrol (3), piceatannol (4), piceatannol-3'-O-β-D-glucopyranoside (5) and isorhapontin (6). Compounds 1-4 inhibited the direct binding between sICAM-1 and LFA-1 of the THP-1 cells in a dose-dependent manner with IC50 values of 50.1, 25.4, 33.4 and 45.9 μM, respectively. On the other hand, the other compounds 5 and 6 with a glucose moiety in each molecule did not show any inhibitory activity in the cell adhesion assay (IC50 values of >100.0 μM). Compounds 2, 3 and 4 also had an inhibitory effect on direct binding between sVCAM-1 and VLA-4 of THP-1 cells. This suggests that the stilbenes from Rheum undulatum rhizomes are good candidates for therapeutic strategies towards inflammation.
Quinolone Alkaloids from Evodiae fructus Inhibit LFA-1/ICAM-1-mediated Cell Adhesion
이승웅,Jong Sun Chang,Ju-Hwan Lim,Min Seok Kim,Su Jin Park,정형재,Min-Soo Kim,이우송,노문철 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.1
Four quinolone alkaloids were isolated by bioactivity-guided fractionation from the methanol extracts of Evodiae fructus fruits. Structures of compounds were elucidated by spectroscopic analysis (1H-, 13C-NMR and MS), as follows:1-methyl-2-undecyl-4(1H)-quinolone (1), evocarpine (2), dihydroevocarpine (3) and mixture of [1-methyl-2-[(Z)-10-pentadecenyl]-4(1H)-quinolone and 1-methyl-2-[(Z)-6-pentadecenyl]-4(1H)-quinolone] (4). They inhibited the interaction of sICAM-1 and LFA-1 in THP-1 cells at IC50 values of >150 (1), 109.8 (2), >150 (3) and 40.9 μM (4), respectively
Inhibition of VLA-4/VCAM-1-mediated Cell Adhesion by Triterpenoid Saponins from Bupleurum falcatum L
이승웅,Min Seok Kim,Ju-Hwan Lim,Jong Sun Chang,Jin Ling,배기환,이우송,노문철 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.7
Discovery and isolation of compounds capable of blocking the interactions between VCAM-1 and VLA-4, a major pair of adhesion molecules contributing to the different steps of leukocyte migration across the endothelium in inflammatory responses, has been a major goal of this lab. Through bioactivity-guided fractionation, five saikosaponins were subsequently isolated from the methanol extracts of the roots of Bupleurum falcatum L. Their structures were elucidated by spectroscopic analysis (1H-, 13C-NMR and 2D-NMR), as follows, saikosaponins: A (1); D (2); C (3); B3 (4); B4 (5). Compounds 1 and 2 inhibited interaction of sVCAM-1 and VLA-4 of THP-1 cells with respective IC50 values of 7.8 and 1.7 μM. The aglycone structure of 2 also showed cell adhesion inhibitory activity with an IC50 value of 21.1 μM. With these results, we suspect these two saikosaponins from the Bupleurum falcatum L. roots to be prime candidates for therapeutic strategies towards inflammation.
Diacylglycerol Acyltransferase에 대한 생약자원의 저해활성 검색
이승웅,고정숙,권오억,이상명,김영호,노문철,김영국,이현선,Lee, Seung-Woong,Ko, Jung-Suk,Kwon, Oh-Eok,Lee, Sang-Myung,Kim, Young-Ho,Rho, Mun-Chual,Kim, Young-Kook,Lee, Hyun-Sun 한국생약학회 2001 생약학회지 Vol.32 No.3
The inhibitory effects of methanol extracts of 135 medicinal herbs on diacylglycerol acyltransferase (DGAT) activity were investigated. DGAT was partially purified from rat liver. Eleven kinds of methanol extracts of medicinal herbs including Evodiae Fructus showed a mild inhibitory effect with the concentration of $125\;{\mu}g/ml$ (above 40% inhibition). Six kinds of methanol extracts including Ephedrae Herba exhibited a weak inhibition. Among them, three kinds of butanol extracts (Sophorae Radix, Arecae Semen, Caesalpiniae Lignum) and the chloroform extracts of Evodiae Fructus showed significant inhibitory activities (above 60% inhibition) at the same concentration.