PDA기반의 산림지리정보 소프트웨어 개발에 관한 연구

석수일 ( Soo Il Suk ),이헌호 ( Heon Ho Lee ),이도형 ( Do Hyung Lee ) 한국산림과학회 2007 한국산림과학회지 Vol.96 No.1

PDA기반의 산림자원조사용 소프트웨어 개발에 관한 연구

이헌호 ( Heon Ho Lee ),이도형 ( Do Hyung Lee ),석수일 ( Soo Il Suk ) 한국산림과학회 2006 한국산림과학회지 Vol.95 No.6

DIAL GAUGE 測定子의 接觸誤差에 關한 硏究

강석수(Suk-Soo Kang),홍준희(Jun-Hee Hong),이동주(Dong-Ju Lee) 한국생산제조학회 2000 한국공작기계학회 춘계학술대회논문집 Vol.2000 No.4

반응성 고분자 개질제를 사용한 아스팔트 콘크리트의 소성변형저감에 관한 연구

장석수(Jang suk Soo),이승한(Lee Seung Han),정용욱(Jung Yong Wook),여인동(Yeo In Dong) 대한토목학회 2007 대한토목학회 학술대회 Vol.2007 No.10

Sol-gel법으로 이산화티탄(TiO₂)을 저온소성 도포시킨 경량골재콘크리트의 아세트알데히드(CH₃CHO) 제거 특성

이승한(Lee Sung Han),여인동(Yeo In Dong),정용욱(Jung Yong Wook),장석수(Jang Suk Soo) 대한토목학회 2011 대한토목학회논문집 A Vol.31 No.2A

최근 대기오염 물질을 제거하기 위하여 TiO₂ 등의 광촉매 재료를 사용한 기능성콘크리트에 대한 연구가 활발히 이루어지고 있다. 이들 연구에서 TiO₂의 흡착은 콘크리트에 직접 혼합하거나, 현탁액을 표면에 직접 도포하는 방법을 사용하고 있다. 이 중 콘크리트에 TiO₂를 직접 혼합하는 방법은 TiO₂의 사용량에 비하여 효능이 떨어져 표면에 직접 도포하는 방법이 많이 이용된다. TiO₂의 표면도포는 광촉매의 활성화와 접착성 증대를 위하여 400℃이상의 고온 열처리를 실시하게 되며, 이는 콘크리트 수회생성물의 탈수 · 수축으로 내부균열을 발생시키는 원인이 되기도 한다. 이에 이 연구에서는 TiO₂의 저온도포가 가능한 Sol-gel법으로 TiO₂를 제조하였으며 펄라이트 사용 경량골재콘크리트에 저온 고정화하여 TiO₂도포 성능을 평가하였다. 또한 펄라이트 입경을 2.5~5.0 ㎜와 5.0 ㎜이상으로 구분하여 펄라이트 입경, TiO₂ 혼입방법과 혼입률 및 시간경과에 따른 CH₃CHO 제거 특성을 검토하였다. 실험결과, Sol-gel법으로 제조한 TiO₂를 120℃에서 저온 도포할 때 XRF 정량분석에서 TiO₂38%, SiO₂ 29%, CaO 18% 순으로 나타나 TiO₂ 도포율은 높게 나타났다. 또한 펄라이트 입경 2.5~5.0 ㎜에서 TiO₂를 저온도포한 경량골재콘크리트의 CH₃CHO 제거 특성은 Sol-gel법으로 제조된 TiO₂를 7% 표면 도포하였을 경우 94%로 나타나 10%를 혼입할 때 72%에 비해 약 20%정도 높게 나타났다. 또한 펄라이트 입경 5.0 ㎜이상에서 TiO₂를 10%로 치환하여 혼합하였을 경우 CH₃CHO 제거율은 69%로 펄라이트 입경 2.5~5.0 ㎜에 대한 72%와 비슷하게 나타나 펄라이트 입경이 CH₃CHO 제거율에 미치는 영향은 크지 않았다. 시간 경과에 따른 CH₃CHO 제거 특성은 전 시험편의 10시간 평균 제거율이 20시간 전체 제거율의 84% 수준으로 나타나 반응 초기에 제거율이 높은 것으로 나타났다. Recently studies on functional concrete with a photocatalytic material such as TiO₂ have actively been carried out in order to remove air pollutants. The absorbtion of TiO₂ from those studies is applied by it being directly mixed into concrete or by suspension coated on the surface. When it comes to the effectiveness, the former process is less than that of the latter compared with the TiO₂ lise. As a result, the direct coating of TiO₂ on materials' surface is more used for effectiveness. The Surface spread of it needs to have a more than 400℃ heat treat done to stimulate the activation and adhesion of photocatalysis. Heat treat consequently leads hydration products in concrete to be dehydrated and shrunk and is the cause of cracking. The study produces TiO₂ used Sol-gel method which enables it to be coated with a low temperature treat, applies it to pearlite using Lightweight Aggregate Concrete fixed with a low temperature treat and evaluates the spread performance of it. In addition to this, the size of pearlite is divided into two types: One is 2.5 ㎜ to 5.0 ㎜ and the other is more than 5.0 ㎜ for the benefit of finding out the removal characteristics of CH₃CHO whether they are affected by pearlite size, mixing method and ratio with TiO₂ and elapsed time. The result of this experiment shows that although TiO₂ produced by Sol-gel method is treated with 120 temperature, it maintains a high spread rate on the XRF(X ray Florescence) quantitative analysis which ranks TiO₂ 38 percent, SiO₂ 29 percent and CaO 18 percent. In the size of perlite from 2.5 ㎜ to 5.0 ㎜, the removal characteristic of CH₃CHO from a low temperature heated Lightweight concrete appears 20 percent higher when TiO₂ with Sol-gel method is spreaded on the 7 percent of surface. In other words, the removal rate is 94 percent compared with the 72 percent where TiO₂ is mixed in 10 percent surface. In more than 5.0 mm sized perlite, the removal rate of CH₃CHO, when TiO₂ is mixed with 10 percent, is 69 percent, which is similar with that of the previous case. It suggests that the size of pearlite has little effects on the removal rate of CH₃CHO. In terms of Elapsed time, the removal characteristic seems apparent at the early stage, where the average removal rate for the first 10 hours takes up 84 percent compared with that of 20 hours.

Telomere 염기배열을 포함하는 DNA , RNA 및 RNA - DNA Hybrid Oligonucleotide 의 구조에 관한 연구

이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5

Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1∼5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence d(T₂G₄)₄. T2, T3, T4 and T5 were d(T₂AG₃)₄, h(U₂dG₄)₄, h(T₂rG₄)₄ and r(U₂G₄)₄, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5`-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer h(U₂dG₄)₄, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer h(T₂rG₄)₄, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer r(U₂G₄)₄ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.

The Pharmaco - chemical Study on the Plant of Ixeris spp. - 1. Anti - hypercholesterolemic Effect of Ixeris sonchifolia

양한석(Han-Suk Young),서석수(Suk-Soo Suh),이경희(Kyung-Hee Lee),이지현(Ji-Hyon Lee),최재수(Jae-Sue Choi) 한국식품영양과학회 1992 한국식품영양과학회지 Vol.21 No.3

고들빼기의 부위별 메탄올 엑스가 고콜레스테롤 생쥐의 혈청 콜레스테롤농도에 미치는 영향을 살펴보았다. 고콜레스테롤 생쥐는 식이에 1% 콜레스테롤과 0.5% 답즙산을 첨가하므로서 유도하였으며, 잎의 메탄올 엑스를 투여한 군은 대조군에 비해 혈청 콜레스테롤 농도를 현저히 감소시켰으나 뿌리의 경우에는 효과가 나타나지 않았다 . 또한, 잎의 메탄올 엑스는 고콜레스테롤 흰쥐에 대해서도 100㎎/㎏ 투여 용량에서 혈청 콜레스테롤 농도를 현저히 감소 시켰으며 동맥경화성 지표를 개선시켰다. 반면에, 정상흰쥐에 대해서는 효과가 나타나지 않았다. 이상과 같은 결과로 잎의 메탄올 엑스는 과잉의 콜레스테롤을 섭취하였을때 체내대사 이용도를 증가시키므로서 혈청 콜레스테롤 농도를 감소시킨다고 생각된다. The methanol extracts from different parts of Ixeris sonchifolia (Compositae) were evaluated for their total cholesterol lowering effect in mice. Mice were rendered hypercholesterolemic with 1% cholesterol and 0.5% cholic acid. Significant lowering in serum cholesterol was observed in mice with the methanol extract from leaves (MeOH-LF), whereas the methanol extract from roots (MeOH-RT) was devoid of this effect. In rats with cholesterol-induced hyperlipidemia MeOH-LF in a dose of 100㎎/㎏ body weight caused significant decrease of total cholesterol, and the atherogenic index was also improved. On the other hand, total cholesterol in rats fed a stock diet was not affected by administration of the MeOH-LF. Thus, it is suggested that this MeOH-LF probably may increase the metabolic utilization only when fed with excess cholesterol.

Structure of Telomeric DNA, RNA and DNA-RNA Hybrid Oligonucleotides by Nondenaturing Polyacrylamide Gel Electrophoresis

이광문,이소영,서석수,이복률,Lee, Kwang-Moon,Lee, So-Young,Suh, Suk-Soo,Lee, Bok-Luel Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.5

Tetrahymena human의 Telomere 염기배열을 가지는 DNA, RNA 및 DNA-RNA hybrid oligonucleotide를 다섯 종류 화학 합성하여 전기영동의 gel mobility shift 실험, Tm 측정 및 DMS methylation 반응으로 그들의 구조를 구명하였다. 합성한 oligonucleotide(T1~5) 의 염기배열은 다음과 같다. T1 : d$d(T_2G_4)_4$, T2 : $d(T_2AG_3)_4$, T3: $h(U_2dG_4)_4$, T4 : $h(T_2rG_4)_4$, T5: $r(U_2G_4)_4$. 이들에 대한 nondenaturing PAGE에서 gel mobility 실험결과 T1과 T2는 size marker DNA 24mer보다 훨씬 빠른 gel mobility를 나타내었고, Tm 측정 및 gel mobility 실험결과 T1은 T2보다 안정한 구조를 취하는 것을 확인하였다. 또한, DMS methylation 실험결과 5'말단의 dG가 methylation반응에 sensitive한 것을 확인하였다. 이상의 결과로서 T1 및 T2 oligoneotide는 용액 중에서 Hoogsteen 염기대를 형성하는 Telmere 구조를 취하는 것을 확인하였고. T1 oligoneotide의 염기배열에서 dT를 rU로 치환한 T3는 T1과 동일구조를 취하는 것이 확인되었으나, dG가 rG로 치환된 T4는 T1과는 다른 gel mobility를 나타내었지만, T5와는 동일한 gel mobility를 나타내었다. 이상의 결과로서 분자내 수소결합으로 Hairpin 구조를 취하는 Telomere 구조형성에 가장 중요한 염기는 deoxyguanosine임을 알았다. Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1~5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence $d(T_2G_4)_4$. T2, T3, T4 and T5 were $d(T_2AG_3)_4$, $h(U_2dG_4)_4$, $h(T_2rG_4)_4$ and $r(U_2G_4)_4$, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5'-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer $h(U_2dG_4)_4$, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer $h(U_2dG_4)_4$, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer$r(U_2G_4)_4$ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.

TMV mRNA 의 in Vitro Translation 을 선택적으로 억제하는 Anti - Sense DNA 및 RNA Oligonucleotide

이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2

Three different antisense oligonucleotides and their RNA analogues, each complementary to 5`-end and 3`-end sequences of Tobacco Mosaic Virus (TMV) mRNA, inhibit in vitro translation of the genomic RNA in a rabbit reticulocyte lysate. Inhibition depends upon the concentration, binding sites of antisense oligonucleotides and the secondary structure of TMV mRNA. DNA oligonucleotides and their RNA analogues complementary to 5`-end regions of TMV RNA have the same effects on inhibition of translation. However, oligonucleotides complementary to 3`-end TMV RNA regions, which are supposed to form pseudoknotted RNA structure, have different effects on inhibition of translation. When DNA antisense oligonucleotides are used for inhibition of translation in wheat germ extract containing RNase H, then they have higher efficiency for inhibition of translation compared to rabbit reticulocyte lysate. We conclude that the design of antisense oligonucleotide depends on the secondary structure of target genes and their intrinsic components.

Channel Catifish Liver 에 중금속 처리로 유도되는 Metallothiolein mRNA 및 Metallothionetionein - Iike 단백질의 합성에 관한 연구

이소영,이광문,서석수,송영환,이복률 ( So Young Lee,Kwang Moon Lee,Suk Soo Suh,Young Hwan Song,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1

When channel catfish was injected with Cd and Zn chloride, the liver of the Cd-treated channel catfish was transiently activated to synthesize metallothionein(MT) mRNA and MT-like proteins. The poly(A)^+ mRNA of about 400 bases long was detected in the Cd-treated channel catfish liver with synthetic DNA probes (27 mer), which were conserved sequences in rainbow trout MT cDNA and human MT cDNA, by northern blot hybridization experiment. Amount of the mRNA from Cd treated channel catfish liver was enough to detect translation products in vitro, using the rabbit reticulocytelysate cell-free translation system. These translation products were MT-like proteins, which were about 6.5 kD in size on SDS-PAGE.