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안면근육 표면근전도 신호기반 특징 및 근육 선별을 통한 단모음인식
이병현(Byeong-Hyeon Lee),류재환(Jaehwan Ryu),이미란(Miran Lee),김상호(Sangho Kim),Md Zia Uddin,김덕환(Deok-Hwan Kim) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6
In this paper, we propose monophthong recognition method using feature and muscle selection based on facial surface EMG signals. We observed that EMG signal patterns may vary according to Korean monophthong pronunciation. The proposed method can recognize Korean monophthong and improve recognition accuracy by selecting muscle and feature rather than by using all of them. Experimental results show that the recognition accuracy of the proposed method using best muscle and best, second features set is better than that using all of muscles and features. The improved average accuracies are 29.13% in kNN, 34.52% in LDA.
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초지 및 조사료 : 이탈리안 라이그래스의 형질전환에 미치는 몇 가지 요인의 영향
이상훈 ( Lee Sang Hun ),우현숙 ( U Hyeon Sug ),이병현 ( Lee Byeong Hyeon ) 한국축산학회 2004 한국축산학회지 Vol.46 No.2
A system for the production of transgenic plants has been developed for Italian ryegrass(Lolium multiflorum Lam) via Agrobacterium-mediated transformation of embryogenic callus. Mature seed-derived calli were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase Ⅱ (NPTⅡ) and intron-containing β-glucuronidase(intron-GUS) genes in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. Inclusion of 200㎛ acetosyringone(AS) in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Increasing Agrobacterium cell density up to 1.0 in OD_(600) during infection increased transformation efficiency of embryogenic calli. The highest transformation efficiency was obtained when embryogenic calli were incoulated with Agrobacterium in the presence of 0.1% Tween20 and 200㎛ AS. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of Italian ryegrass.
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초지 및 조사료 : 배지첨가물질이 이탈리안 라이그래스의 종자유래 캘러스 배양에 미치는 영향
우현숙 ( U Hyeon Sug ),이병현 ( Lee Byeong Hyeon ) 한국축산학회 2004 한국축산학회지 Vol.46 No.2
In an effort to optimize tissue culture responses of Italian ryegrass(Lolium multiflorum Lam.) for future genetic manipulations to improve forage characteristics, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of three cultivars, `Jeanne`, `Florida-80` and `Metro`, as explant tissues. For all explants, MS medium containing 5㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The optimal concentration of dicamba for the induction of embryogenic callus from mature seeds was 7㎎/L. The highest plant regeneration frequency was observed when embryogenic callus was transferred to N6 medium supplemented with 1㎎/L 2,4-D and 5㎎/L BA. Plant regeneration frequency of callus cultured in the dark was higher than that of cultured in the light. Casein Hydrolysate and L-proline improved both in empryogenic callus induction from mature seeds and plant regeneration. High-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.
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