http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
사람 뇨의 γ - Glutamyl Transpeptidase
김호중,이민화 ( Ho Jung Kim,Min Wha Lee ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.3
A crude urinary protein concentrate was prepared by treating human urine with amrnonium sulfate, and was found to be active in hydrolyzing the synthetic substrate, r-glutamyl-p-nitroanilide. The hydrolytic activity of the urine enzyme was most efficient at pH 8.5 in tris buffer in the presence of glycylglycine as a r-glutamyl acceptor. L-Glutamine and L-methionine also acted as a r-glutamyl acceptor, but L-glutamic acid, L-arginine, glycine, L-valine, L-aspartic acid, and L-homoserine inhibited the enzyme, suggesting that these amino acids do not act as r-glutamyl acceptors. The enzyme was markedly inhibited by bromosulfophthalein, oxidized glutathione, and by L-serine in the presence of borate, the inhibition being more marked than is the cage with human serum and rat kidney enzymes. L mike rat kidney enzymes, the urine enzyme did not show phosphate-independent and maleate-stimulated glutaminase activity. The urinary enzyme activity was not correlated with the serum enzyme activity in various disease entities such as liver disease, kidney and urinary tract infections, and others.
K-BEtestⓡ , 새로운 생물학적 동등성 시험 통계처리 프로그램의 개발
이영주(Young Joo Lee),최정호(Jung Ho Choi),송세흠(Sae Heum Song),서철환(Chul Hwan Seo),김동섭(Dong Sup Kim),박인숙(In Sook Park),최기환(Ki Hwan Choi),나한광(Han Kwang Na),정석재(Suk Jae Chung),이민화(Min Hwa Lee),심창구(Chang Koo Shi 한국약제학회 1998 Journal of Pharmaceutical Investigation Vol.28 No.4
N/A A computer program for personal computers, K-BEtest^ⓡ, was developed to analyze bioequivalence data in accordance with Korean Guidelines for Bioequivalence Test (KGBT). This program is user-friendly, interactive, Hangul-compatible and supports 2×2 cross-over design as well as 2×2 Latin square design with various significance levels. This program is able to calculate AUC, C_(max) and T_(max) parameters from the blood drug concentration-time profile of individual subjects and evaluate the parameters statistically for the bioequivalence by ±20% rule, the F-test, the Non-centrality test and 90% confidence intervals. All procedures are supported with graphic interface, interactive menu and outputs in Korean. In this paper, two experimental data sets were analyzed by the program and detailed process was demonstrated. The K-BEtest^ⓡ program appears to be very effective for analyzing bioequivalence data and can be widely used with convenience and accuracy.
$K-BEtest^{\circledR}$, 새로운 생물학적 동등성 시험 통계처리 프로그램의 개발
이영주,최정호,송세흠,서철환,김동섭,박인숙,최기환,나한광,정석재,이민화,심창구,Lee, Young-Joo,Choi, Jung-Ho,Song, Sae-Heum,Seo, Chul-Hwan,Kim, Dong-Sup,Park, In-Sook,Choi, Ki-Hwan,Na, Han-Kwang,Chung, Suk-Jae,Lee, Min-Hwa,Shim, Chang-K 한국약제학회 1998 Journal of Pharmaceutical Investigation Vol.28 No.4
A computer program for personal computers, $K-BEtest^{\circledR}$, was developed to analyze bioequivalence data in accordance with Korean Guidelines for Bioequivalence Test (KGBT). This program is user-friendly, interactive, Hangul-compatible and supports $2{\times}2$ cross-over design as well as $2{\times}2$ Latin square design with various significance levels. This program is able to calculate AUC, $C_{max}$ and $T_{max}$ parameters from the blood drug concentration-time profile of individual subjects and evaluate the parameters statistically for the bioequivalence by ${\pm}20%$ rule, the F-test, the Non-centrality test and 90% confidence intervals. All procedures are supported with graphic interface, interactive menu and outputs in Korean. In this paper, two experimental data sets were analyzed by the program and detailed process was demonstrated. The $K-BEtest^{\circledR}$ program appears to be very effective for analyzing bioequivalence data and can be widely used with convenience and accuracy.
이민화,김호중,최원기 생화학분자생물학회 1984 BMB Reports Vol.10 No.4
Intact rabbit erythrocytes were radioiodinated by lactoperoxidase method so that only the outer surface membrane proteins be labeled. After iodination, the isolated membranes were analyzed for their polypeptide and radioactivity profile by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Of the two radiolabeled polypeptides of the membrane, only one radioactive peak corresponding to Band 3 polypeptide(Steck, 1971) was solubilized in part by buffered 1 M urea, with the concomitant release of the ecto-NAD glycohydrolase from the membrane. The other, Band 3-solubilizing agents such as pancreatic lipase and Triton X-100 also solubilized the enzyme. In contrast, after treating the membrane with 10% acetic acid, which selectively elutes a group of the peripheral proteins of the cytoplasmic surface, leaving Band 3 intact in the membrane, one-half of the enzyme activity remained still associated with the residual membrane. From these results, the association of the ecto-NAD glycohydrolase with Band 3 component, the predominant transmembrane polypeptide of the rabbit erythrocyte membrane is proposed.