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連翹敗毒散이 사람 기관지 상피세포의 TARC 분비에 미치는 효과
이경엽,김희택,김이화,남창규,류주현,Lee, Kyung-yeob,Kim, Hee-taek,Kim, E-hwa,Nam, Chang-gyu,Ryu, Ju-hyun 대한한방안이비인후피부과학회 2003 한방안이비인후피부과학회지 Vol.16 No.3
Chemokines are important for the recruitment of leukocytes to sites of infection, which is essential in host defense. The thymus and activation-regulated chemokine (TARC) is a CC chemokine which potentially plays a role via a paracrine mechanism in the development of allergic respiratory diseases. Objectives : The objective of this study is to investigate the effect of Youn-Gyo-Pae-Doc-San on the secretion of TARC of human bronchial epithelial cell Methods : Enzyme-linked immunosorbent assay (ELISA) was performed to detect the secretion of TARC. The cytotoxicity was measured by MTT assay. Results : Youn-Gyo-Pae-Doc-San significantly inhibited the secretion of TARC with a dose -dependant manner. The effective dosage did not have the cytotoxicity on human bronchial epithelial cell. Conclusions : Results of our study show that Youn-Gyo-Pae-Doc-San would play an important role in modulation of TARC in human bronchial epithelial cells.
정향 추출물 도포가 DNCB로 유발된 알레르기성 접촉 피부염에 미치는 영향
이경엽,강다혜,김희택,Lee, Kyung-Yeob,Kang, Da-Hae,Kim, Hee-Taek 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.4
Objectives : The purpose of this study is to investigate effects of Syzygium aromaticum (SAE) spread on the allergic contact dermatitis caused by 2,4-dinitro-chlorobezene (DNCB). Methods : Forty-two mice were divided into six groups ; normal, negative control (DNCB-treated), positive control (DNCB + 1% pimecrolimus), experimental group I, II and III. control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group I(DNCB + 0.2% SAE), II(DNCB + 1% SAE) and III(DNCB + 5% SAE) were spread SAE and positive control was spread the 1% pimecrolimus. In this study, effect of SAE on clinical aspects on the skin, histopathological change, the blood level of IgE, cytokines, histamine were investigated. In addition, effect of SAE on spleen $CD4^+/CD8^+$ T cell subset was investigated. Results : 1. In experimental group I, II and III, erythemas and edema were more reduced than negative control. 2. In experimental group I, II and III inflammatory edema and the numbers of infiltrated inflammatory cells were more reduced than negative control. 3. In experimental group I, II and III, clinical skin score was more reduced than negative control. 4. In experimental group II and III, the thickness of skin was statistically significant reduced than negative control. 5. In experimental group II and III, histamine release was statistically significant reduced than negative control in dose-dependantly. 6. In experimental group II and III, cytokines (IL-1${\beta}$, TNF-${\alpha}$, IL-4, IL-6, IL-10) were statistically significant reduced than negative control in dose-dependantly. 7. In experimental group I, II and III, the level of total IgE was statistically significant reduced than negative control in dose-dependantly. 8. In experimental group III, $CD4^+$ and $CD8^+$ T cells were statistically significant decreased similar to the positive control. Conclusions : According to above experiments, Syzygium aromaticum(SAE) was effective on allergic contact dermatitis.
플라즈마방전 시뮬레이션을 위한 C₄F? 분자기체의 초기전자충돌 단면적 개발
류선미(Sun-Mi Ryu),이경엽(Kyung-Yeob Lee),조두용(Doo-Yong Jo),전병훈(Byung-Hoon Jeon) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.7
For quantitative understanding of gas discharge phenomena, we should know electron collision cross section. Processing plasma etching of semiconductor, and research are being used in the etching source C₄F? gas may be used by itself and mixed with other gases are also used. However, the molecular gas C₄F? study on the characteristics of the electron transport and the cross-sectional area of the decision is still lacking. Therefore, we understand the electron transport characteristics and analysed the electron transport coefficients. And to understand and interpret physical properties of the ionization coefficient α/N, and the attachment coefficient η/N in C₄F? gas.