단순포진 Virus 감염의 세포학적 진단시 면역조직 화학법의 적용

박혜림,이갑노,백승룡,Park, Hye-Rim,Lee, Kap-No,Paik, Seung-Yong 대한세포병리학회 1990 대한세포병리학회지 Vol.1 No.1

Herpes simplex virus type 1 and 2(HSV-1, HSV-2) are the ubiquitous human pathogens responsible for a variety of afflictions. HSV-2 is one of the viruses that were suspected of promoting carcinogenesis in the uterine cervix. Certainly, there is a need for the more sensitive and accurate laboratory techniques for HSV detection. We examined total 80 cases of smears including 17 Tzanck smears of skin and 63 cases of Papanicolaou smears from total 77 patients with clinical impression of herpetic infections, from September, 1985 through August, 1989. Immunohistochemical typings for HSV-1 and HSV-2 were performed together with routine cytologic findings and compared. The results are as follows : 1) Patients were 9 males and 33 females, and age distribution was between 5 and 71 years. 2) Subjective symptoms such as ulceration, vesicle, vaginal discharge, pruritus, and pain were complained in 36 patients and 38 cases were genital herpes. Recurrence was noted in 11 cases. 3) Positive results were obtained in 42 among 80 cases. 4) Both routine cytology and immunohistochemical staining were positive in 13 cases and in 24 cases only immunohistochemical staining were positive. 5 cases were positive only in routine cytologic smears. 5) The cases that immunocytochemical stain had been performed were 37 cases, which were all positive in type 2. Among the above 37 cases, type 1 also were positive in 5 cases. The results show that the immunoperoxidase technique is one of the rapid and reliable method to confirm the herpetic infection when suspected and that it is particularly useful when the Papanicolaou smear findings are equivocal.

중위부 직장암의 하방 내벽 침습도에 관한 병리조직학적 고찰

이승규(Sung Gyu Lee),황정웅(Cheung Wung Whang),서성옥(Sung Ock Suh),배정원(Cheung Won Bae),민병철(Pyung Chul Min),이갑노(Kap No Lee) 대한외과학회 1986 Annals of Surgical Treatment and Research Vol.31 No.2

휴대형 유헬스케어 의료기기 평가방법

남명현(Myung-Hyun Nam),김수찬(Soo-Chan Kim),김장수(Jang Su Kim),이갑노(Kap No Lee),김산(San Kim),차지훈(Ji Hun Cha),허찬회(Chan-Hoi Hur),박기정(Ki-Jung Park) 大韓電子工學會 2012 電子工學會論文誌-SC (System and control) Vol.49 No.2

정보기술과 의료기기의 융합에 의해 혈당을 집에서 측정하거나 운동 시에 심박수를 측정하는 것과 같이 건강관련 정보를 언제 어디서나 측정할 수 있게 되었고, 이에 따라 의료를 장소에 구애받지 않고 쉽게 접근할 수 있게 되었다. 최근에 유헬스케어 의료기기 시장이 빠르게 성장하고 있으나 이러한 기기의 안전성과 유효성을 평가하는 가이드라인이 아직 마련되지 않은 실정이다. 이를 위해 식약청, ISO/IEEE, CE 등 국내외의 유헬스케어 관련 규정을 조사하고, 이 중 유헬스케어 의료기기에 관련된 안정성 및 성능 평가에 대한 시험 방법과 항목을 제안하였다. 유헬스케어 의료기기의 가장 큰 특성인 무선 데이터 통신에 있어 데이터 무결성과 기기 사용환경이 다양하기 때문에 환경 요인이 기기에 미치는 영향을 평가하였다. 이를 바탕으로 휴대형 유헬스케어 의료기기의 평가 가이드라인을 제안하였다. 제안하는 지침을 기반으로 휴대형 유헬스케어 기기를 개발한다면 사용자들에게 보다 안전성과 신뢰도 높은 기기를 제공할 수 있을 것으로 기대된다. Convergence of information technology (IT) and medical devices enables people to measure health-related information ubiquitously, such as measuring blood glucose at home and checking cardiac signals during exercise and it allows us to access to medical care anywhere and anytime. Nowadays, the market for U-healthcare medical devices is growing rapidly, but guidelines for the evaluation of safety and effectiveness of such devices remain to be formulated. We performed a study on the development of safety and performance evaluation method for portable, hand-held, U-healthcare medical devices. We reviewed current guidelines and standards for home-health devices from the Korea Food and Drug Administration (KFDA) and related international committees such as the ISO/IEEE and CE. We summarized the test methods and items for the evaluation of safety and performance related to U-healthcare medical devices from the above guidelines and standards. We defined requirements for a U-healthcare medical device to demonstrate good performance. In conclusion, we propose an evaluation method for U-healthcare medical devices, which will help improve the safety and reliability of these devices.

소아에서 ABO 혈액형 부적합 간이식술의 치험

송태진(Tae Jin Song),임채승(Chae Seung Lim),박인승(In Soong Park),이갑노(Kap No Lee),정기훈(Ki Hoon Jung),최상용(Sang Young Choi),문홍영(Hong Young Moon),구범환(Bum Hwan Koo) 대한외과학회 1998 Annals of Surgical Treatment and Research Vol.55 No.4

관상동맥질환 환자 , 운동선수 및 정상 대조군에서 혈청지질 및 Apolipoproteins 에 대한 연구

박정의(Jeong Euy Park),김우주(Woo Joo Kim),최인석(In Suok Choi),김대원(Dae Won Kim),이갑노(Kap No Lee),김성수(Sung Soo Kim),유원상(Won Sang Yoo),최석구(Suck Koo Choi),이건주(Kun Joo Rhee),서순규(Soon Kyu Suh),이영(Young Lee),고영박(You 대한내과학회 1991 대한내과학회지 Vol.40 No.5

N/A To investigate the usefulness of serum lipids and apolipoproteins as indices of risk factors for coronary artery disease (CAD) and to know the effects of regular exercise on the serum lipids and apolipoproteins, we measured the serum lipids and apolipoproteins in the patients with coronary artery disease, athletes and normal control persons. The study subjects included 67 patients with CAD (57. 8±11.2yr), 22 athletes (21.5±2.1yr) and 62 normal control persons (34.3±11.4yr). 1) In the patients with CAD the serum total cholesterol (TC) was 177. 2±38.5 mg/dl and the HDL-cholesterol (HDL-C) was 39.0±11.2mg/dl. In the normal control persons TC was 162.5±33.5 mg/dl and HDL-C was 42.4±12.6mg/dl, which were not significantly different from the values of CAD patients. 2) The serum apolipopratein A (Apo-A) was significantly lower (201.9±39.6mg/dl, p<0.05), and the apolipoprotein B (Apo-B) was significantly higher (132.3±29.7 mg/dl, p<0.05) in the patients with CAD, compared with normal control persons (age over 40 years; Apo-A: 246.3±39.9 mg/dl, Apo-B: 117.9±26.4 mg/dl). 3) The TC/HDL-C ratio and Apo B/A ratio were significantly higher (4.9±1.6, 0.67±0.17, p<0.005 for TC/HDL-C ratio and p<0.05 for Apo B/A ratio) in the CAD patients compared with normal control persons (age over 40 years; 3.6±1.1, 0.49±0.10). 4) The TC/HDL-C ratio ≥4.0 and Apo B/A ratio ≥0.55 were the good discriminating criteria separating the CAD patients from the normal control persons. 5) In the athletes, the serum triglyceride (TG) was significantly lower (65.7±20.7mg/dl, p<0.001), and the HDL-C was significantly higher (57.8±11.2 mg/dl, p<0.001) compared with normal control persons (age under 30 years; TG; 119.8±94.3 mg/dl, HDL-C: 44.6±11.0mg/dl). The Apo-A, Apo-B, TC/HDL-C ratio and Apo B/A ratio were all significantly lower (p<0.01) compared with those in the normal control persons. 6) Among the athletes, the HDL-C was highest and the TG was lowest in the swimmers compared with fencing players and wrestlers. The Apo B/A ratio and TC/HD1-C ratio were better discriminating indices of risk factors for CAD compared with individual lipids or apolipoprotein values. The Apo-A and Apo-B were considered to be good indices of risks for CAD. The athletes had lowest risks for CAD.

Campylobacter pylori 감염의 비관혈적이고 신속한 진단을 위한 14C - urea Breath Teat

박영태(Young Tae Bak),김진호(Jin Ho Kim),김종극(Jong Guk Kim),이창홍(Chang Hong Lee),박승철(Seung Chull Park),조윤정(Yun Jeung Cho),김대원(Dae Won Kim),이갑노(Kap No Lee) 대한내과학회 1988 대한내과학회지 Vol.34 No.5

N/A During the recent several years, Campylobacter pylori organisms have been implieated in the gastroduodenal diseases including gastritis, duodenal ulcer, gastric ulcer, epidemic hypochlorhydria, nonulcer dyspepsia, etc. Diagnositc methods include culture of the organism, detection of the organism in the gastric mucosa, demonstrating urease activity and serology. Endoscopy with biopsy have been required for the detection methods. In an effort to avoid the expense and discomfort of endoscopy in patient selection and follow-up studies, 14C-urea breath test was done and compared with the other diagnostic methods. Campylobacter pylori culture and histological examination of gastric antral mucosa, Gram stain of gastrie juice, and 14C-urea breath test was done in 123 cases undergoing upper gastrointestinal endoscopic examinations. Diagnostic sensitivity of the urea breath test with cut-off value of 0.4% was 0.93, specificity was 1.00, and efficiency was 0.94%. Among the diagnostic tests for C. pylori infection, detection with Warthin-Starry silver stain and 14C-urea breath test were most efficient (0,92 & 0.94), In conclusion, 14C-urea breath test may be a simple, noninvasive, rapid and very efficient method for the detection of C. pylori infection of the stomach.

임상병리 전산화의 그 실제(Ⅴ) : 다종의 자동분석기를 PC와 접속(Interface)후 이를 병원 전산기와의 접속에 관한 연구

이갑노 대한임상병리학회 1992 대한임상병리학회지 Vol.12 No.2

2% NaCl Mueller-Hinton Agar와 Mannitol Salt Agar를 이용한 황색포도구균의 Methicillin 내성 검출

마경란,이도현,이창규,황선철,김영기,이갑노 대한감염학회 1998 감염 Vol.30 No.6

목 적 : MRSA 중 heteroresistant MRSA 는 온도, 배양시간, 배지 내 염농도 등 다양한 환경 조건에 영향을 받는 것으로 알려져 있다. 이에 저자들은 oxacillin 디스크를 이용하여 2% NaCl이 첨가된 Mueller-Hinton agar 와 mannitol salt agar 에서의 methicillin 내성을 평가하여 보았다. 방 법 : 70주의 S. aureus 에 대해 MH agar, 2% NaCl MH agar, MSA에서 1μg oxacillin 디스크를 이용한 디스크 확산법을 시행하였다. 또한 oxacillin의 MIC를 E-test로 시험하였다. 표준 방법으로 mecA 유전자를 PCR로 증폭하고 agarose gel 에서 전기영동하여 확인하였다. 결 과 : 일반 MH agar 는 12주(18%)의 S. aureus 에서 heterogeneous resistance 를 검출할 수 없었으나, 2% NaCl MH agar 와 MSA homogeneous 와 heterogeneous resistance 를 올바르게 검출할 수 있었다. 변 검체에서는 48%, 변이 아닌 검체에서는 5% 의 heterogeneous resistance 를 보였다. 결 론 : 일반 검사실에서 황색포도구균의 methicillin 내성의 정확한 검출에 2% NaCl MH agar와 MSA가 유용하게 사용될 수 있음을 알 수 있었다. Background : Methicillin-resistant Staphylococcus aureus (MRSA) has a heteroresistant nature, so methicillin resistance is influenced by various culture conditions, such as temperature, incubation time, and NaCl content in the medium. Mueller Hinton (MH) agar containing 2% NaCl and mannitol salt agar (MSA) with oxacillin disk were evaluated for the detection of methicillin resistance. Methods : Disk diffusion test on plain Mueller-Hinton (MH) agar, 2% NaCl MH agar, and MSA with 1 μg oxacillin disk was performed in 70 Staphylococcus aureus isolates. Oxacillin MIC was determined by E-test. As a gold standard of methicillin resistance, mecA gene was amplified by PCR and detected by agarose gel electrophoresis. Results : Plain MH agar could not detect heterogeneous resistance in 12 S. aureus isolates (18%), but 2% NaCl MH agar and MSA could correctly detect homogeneous and heterogeneous resistance. S. aureus isolates from stool have as much as 48% heterogeneous resistance, while those from non-stool specimen have 5%. Conclusion : 2% NaCl and MSA can be used reliably for accurate susceptibility testing of methicillin resistance in routine laboratory.

삼일열 말라리아 치료효과 추적에 있어 혈소판 지표들의 의의

임채승,김영기,이갑노 대한감염학회 1998 감염 Vol.30 No.5

목적: 한국의 말라리아는 점차 증가하여 1997년만 1600명 이상이 발생하였는데 말라리아의 확산을 방지하기 위해서는 매개모기의 조절 뿐 아니라 조기진단과 조기치료가 필요한 상황이다. 현재까지는 후충 혹은 박층의 말초도말혈액에 Giemsa 혹은 Wright 염색을 통한 검경이 고전적인 방법이 항말라리아제 치료 후 효과추적에 사용되고 있는데 많은 경험을 필요로 하는 단점이 있다. 혈소판 감소증은 삼일열 말라리아 환자에서 흔히 관찰되는 현상중 하나인데 저자들은 쉽고 경제적인 지표로서 항말라리아 치료 효과 추적에 있어 혈액학적 지표의 의의를 연구 평가하였다. 방법: 저자들은 군 병원에 입원한 말라리아 환자 22명의 치료전 후 혈액을 이용하여 박층, 및 후층도말을 만들어 Giemsa나 Wright Giemsa 염색을 실시하였고 동시에 자동 혈액측정기인 Celldyn 3000을 이용하여 10개 항목의 혈액검사를 실시하였다. (WBC, RBC, Hb, Hct, MCV, MCH, MCHC, RDW, PLT, MPV). 이 결과를 바탕으로 항말라리아제 치료전후 원충 농도와 혈액학적 지표들과의 상관관계를 분석하였다. 결과: 모든 혈액학적 지표에서 항말라리아제 치료전 후 유의한 변화는 관찰되지 아니하였으나 혈소판 수치는 정상으로 회복하였고 혈중 원충 농도와 역상관 관계를 보였다. 결론: 말라리아 환자에서 혈소판 수치는 치료효과추적에 있어 간단하고 도움이 되는 지표로 사용될 수 있을 것이다. Background: In Korea, vivax malaria has progressively increases to 1,700 cases in 1997. To prevent the spread of malaria, early detection and treatments, as well as vector control programs, are essential. Until now, classic methods, such as thin and thick blood smears with Giemsa or Wright stain have been routinely used as follow-up after anti-malarial treatment, but these require expertise. We evaluated here hematologic indices as makers in follow-up after anti-malarial treatment. Methods: Thin and thick blood smears were prepared in 22 patients at pre-and posttreatment periods. The slides were stained with Giemsa or Wright Giemsa solution. Simultaneously complete blood cell counts including 10 hematologic indices (WBC, RBC, Hb, Hct, MCV, MCH, MCHC, RDW, Platelet, MPV) were done by autoanalyser Celldyn 3000. We analysed correlation between parasitemia and each hematologic index and significant changes between pre-and posttreatment. Results: Other indices were not significantly changed after anti-malarial treatment, except for platelet counts which inversely correlated with parasitemia. Mean platelet counts of patients were recovered from 97,000/uL to 14,1000/uL in posttreatment follow-up. Conclusion: Platelet counts in malaria patients could be used as simple and useful marker for postreatment follow-up.

고려대학교 의료정보 Network System에서 검사실 정보처리 시스템 (Ⅰ)

이갑노,윤수영 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.4