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Microsatellite DNA 를 이용한 진돗개 ( Canis familiaris ) 집단의 유전적 구조에 관한 연구
윤신근(Sin Keun Youn),김익수(Ik Soo Kim),손흥대(Hung Dae Sohn) 한국유전학회 2001 Genes & Genomics Vol.23 No.1
N/A Characteristics of population genetic structure of the Jindo dog breed inhabiting Korean mainland and the offshore islet, Jindo, where the name of the dog originated, were compared using four microsatellite DNA loci. Considerably higher genetic diversity estimates and allele numbers were observed in the Jindo islet population, indicating genetic heterogeneity in this population. This is quite different from what one can expect because currently much less numbers of Jindo dogs are raised in Jindo islet (∼10,000 in Jindo islet vs. ∼100,000 in mainland). We discussed the observed result in terms of non-random breeding in the mainland population and possible release of originally pure dogs to mainland. Although the mainland population showed a significant deviation from Hardy-Weinberg equilibrium, higher homozygosity, and highly positive FIS in two loci, the Jindo islet population revealed these phenomena only in one locus, suggesting that Jindo dogs in mainland propagate more severely through small numbers of dogs. This non-random breeding also appears to be responsible for the differentiated gene pool between the two populations.
박준원,지향,김은옥,윤신근,김대용,오원석,우계형 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.4
Granular cell tumor was described in a 7-year-old male Miniature Pinscher. Sublingual mass was surgically removed and submitted for diagnosis. The mass was about 1.8 cm in diameter, firm, tan, and ulcerated. On histopathology,the mass was unencapsulated and infiltrated adjacent tissue and consisted of nest or sheet of round to polygonal, and occasional spindle-shaped cells. The neoplastic cells contain large amount of eosinophilic granular materials in the cytoplasm. Immunohistochemically, the neoplastic cells were positive to neuron-specific enolase but were negative to S-100. The cytoplasmic eosinophilic granules were positive to PAS but were negative to PTAH methods. Based on these results, the mass was diagnosed as sublingual granular cell tumor. No recurrence or metastasis was noted so far since surgery.
서울지역에서 문제되고 있는 강아지의 급성설사증에 관한 병인학적 연구
한홍율,박희명,이진희,오태호,정순욱,윤신근,박철만,Han Hong-Ryul,Park Hee-Myung,Lee Jin-Hee,Oh Tae-Ho,Jeong Soon-Wuk,Youn Sin-Keun,Park Cheol-Man 한국임상수의학회 1993 한국임상수의학회지 Vol.10 No.2
In Seoul area, there are so many kennel clubs, veterinary hospital, pet establishment and breeding confers that various problems have occurred. They are crowed pet houses, poor sanitation, stress to puppies, sudden environmental changes to puppies and unvaccination against parvovirus, canine distemper virus, parainfluenza virus, infectious hepatitis caused by Adenovirus type I and Leptospira. Several studies were made to survey the infectious agents involved in acute diarrhea of poppies in Seoul are, such as history taking, physical examination, complete blood count and serum chemistry, histopathological finding, bacterial isolation and identification, and hemagglutination test in feces and hemagglutination inhibition test in serum against parvovirus, respectively. The results obtained are summarized as followed. 1. The percent of PCV (30.5$\pm$5.6) and concentration of Total protein(5.0$\pm$0.8) resulted from statistical analysis are significantly lower than normal values (p<0.05), respectively. In addition, fibrinogen (505$\pm$326) was significantly higher an normal value (p<0.001), Band neutrophil (22.9$\pm$12.7) showed signifiant difference (P<0.01). decreased monocyte (3.2$\pm$2.1) and eosinophil (0.7$\pm$0.8) values appeared statistically significant (p<0.001), lymphocyte (16.7$\pm$9), as well. 2. The concentrations of calcium .(8.0$\pm$2.8), glucose (40.1$\pm$31.4), and albumin (2.0$\pm$0.39) were lower than normal values (p<0.01, p<0.01, p<0.001), respectively. Also Inorganic phosphate (7.1$\pm$2.4), pH (p<7.9f:0.2), and Blood Urea Nitrogen (40.41=37.1) were significantly higher than normal values (p<0.001, p(0.001, p<0.05), respectively. 3. Simple and mixed infections occupied 18% and 82% in the distribution of causes in puppies with acute diarrhea, respectively. 4. As puppies got older, incidence of acute diarrhea caused by Staphylococcus aureus was decreased to 13% and infection of canine distemper virus was increased to 53%, but E coli and canine parvovirus always showed high frequency of outbreak in the body weight ranged from 35g to 7.8Kg. 5. As showed in table 5, infections of E coli and Canine Parvovirus showed high outbreak regardless of the age which is classified into three stages, 35~50 day, 60 day and 75~day to 1 year, canine distemper virus appeared increased, but in case of Staphylococcus aureus, visa versa. 6. In comparison wi methods for the laboratory diagnosis diagnosis parvovirus, Hemagglutination test showed positive reaction in 25% and mean serum antibody titer measured by Hemagglutination inhibition test showed 2779 (n=20). In addition, positive reaction was 90% (18/20). 7. In histopathological studies, enteritic and pneumonic lesions were indicated in 53.7% and 39.5% of samples, respectively. 8. Etiologic diagnosis based on the history taking, clinical signs and histopathological findings in puppies with acute diarrhea and vomiting indicated that 50% of puppies were infected by canine parvovirus and distemper virus. 9. In the parasitological examination made by simple flotation with saturated zinc sulfate tour parasites found were Isospora canis, Toxocara canis, Ancylostoma spp and Toxocara spp. Isospora canis and Toxocara canis were more frequently found among those parasites of diarrhoeic causes in puppies ranged from 35 days to 1 year. Their infestation rates were 15% and 13% respectively.