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다제내성 Staphylococcus aureus 에 항균활성을 나타내는 CNU30122 균주가 생산하는 항생물질
윤봉식(Bong Sik Yun),조수묵(Soo Muk Cho),김창진(Chang Jin Kim),유익동(Ick Dong Yoo) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.6
During the screening for the antimicrobial agents against multidrug-resistant Staphylococcus aureus, we isolated an active compound produced by strain CNU30122. The active compound was purified from culture broth by HP-20 column chromatography, ethylacetate extraction. silica gel column and Sephadex LH-20 column chromatographies and HPLC. Based on various NMR studies including ¹H-¹H COSY. ¹H-^(13)C COSY and HMBC experiments. the active compound was identified as fusidic acid.
Streptomyces sp. 50634 균주가 생산하는 tipA Promotor 활성화 물질, Sulfomycin Ia
심용호,윤봉식,세또 하루오,황세영,유익동,Shim, Yong-Ho,Yun, Bong-Sik,Seto, Haruo,Hwang, Se-Young,Yoo, Ick-Dong 한국미생물 · 생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.6
In the course of screening for the tipA promoter-inducing substances, we isolated an active compound, sulfomycin Ia, from the mycelium of a microorganism designated 50634. The producing organism was identified as Streptomyces sp. on the basis of taxonomic studies. Sulfomycin Ia was purified from mycelial extract by silica gel column chromatography, LH-20 column chromatography, silica gel TLC, and preparative HPLC. The molecular weight of sulfomycin Ia was determined to be m/z 1129 (M+Na)$^{+}$ by FAB mass measurement and $^{1}$H NMR spectroscopic analysis. The structure was assigned as a derivative of sulfomycin I with thiazole, methyloxazole, oxazole, and pyridine rings by $^{1}$H NMR spectral data.
이인경,윤봉식,김종평,정성현,심규섭,유익동,Lee, In-Kyoung,Yun, Bong-Sik,Kim, Jong-Pyung,Chung, Sung-Hyun,Shim, Gyu-Seop,Yoo, Ick-Dong 한국생약학회 1998 생약학회지 Vol.29 No.3
Seven antioxidative compounds were isolated from chloroform and ethyl acetate extracts of the stem bark of Eucalyptus globulus (Myrtaceae). They were identified as rhamnazin (1), rhamnetin (2), naringenin (3), eriodictyol (4), quercetin (5), taxifolin (6) and dihydrokaempferol-3-rhamnoside (7) on the basis of various spectroscopic analyses. These compounds inhibited lipid peroxidation with $IC_{50}$ values of 0.08-30 ${\mu}g/ml$.
양지꽃(Potentilla fragarioides) 지상부의 항산화물질
최용화,김명조,이행순,윤봉식,호창서,곽상수,Choi, Yong-Hwa,Kim, Myong-Jo,Lee, Haeng-Soon,Yun, Bong-Sik,Hu, Changxu,Kwak, Sang-Soo 한국생약학회 1998 생약학회지 Vol.29 No.2
Six antioxidative compounds in the aerial parts of Potentilla fragarioides were isolated by a bioassay guided purification using a DPPH free radical. They were identified as (+)-catechin, isoquercitrin, quercitrin, $quercetin-3-O-{\beta}-D-glucopyranosyl-{\beta}-D-xylopyranoside$, caffeic acid, and 4-O-caffeoyl-L-threonic acid on the basis of $^{1}H$ and $^{13}C-NMR$ and MS data. The DPPH radical scavenging activity of five compounds $(RC_{50}:\;7.5{\sim}10.5\;{\mu}g)$ except for quercitrin $(16\;{\mu}g)$ was more effective than those of ${\alpha}-tocopherol$ $(12\;{\mu}g)$ and BHA $(14\;{\mu}g)$.
이인경,유인자,정동호,한규훈,윤봉식,유익동,Lee, In-Kyoung,Ryoo, In-Ja,Choung, Dong-Ho,Han, Kyou-Hoon,Yun, Bong-Sik,Yoo, Ick-Dong 한국생약학회 1997 생약학회지 Vol.28 No.3
Hibiscus syriacus L. (Malvaceae) is widely distributed over Korean, China, India and Siberia. The dried flower of Hibiscus syriacus is used as a folk medicine for curing of hematochezia, dysentery, obstruction due to wind-phlegm, regurgitation, and vomiting of food, and the dried root bark is used antipyretic, anthelmintic and antifungal agents. From a chloroform extract of root bark of this plant, compound I, II, and III were isolated and the structures were elucidated by various spectroscopic analyses. These compounds were identified as syringaresinol. E-N-feruloyltyramine, and Z-N-feruloyltyramine, respectively and were isolated from this plant for the first time. Compound II and III exhibited lipid peroxidation inhibitory activities with $IC_{50}$ of 15.5 and 28.6 ${\mu}g/ml$, respectively.
김은실(Eun Sil Kim),윤봉식(Bong Sik Yun) 한국디자인문화학회 2011 한국디자인문화학회지 Vol.17 No.4
본 연구의 목적은 교토(京都) 하나마치를 중심으로 일본 문화자원의 꽃이라 할 수 있는 게이샤의 메이크업과 의상 및 기타 액세서리를 중심으로 그 외적이미지를 분석함으로써 경제적 가치와 문화적 가치로서의 게이샤를 재조명하는데 그 목적이 있다. 연구방법은 이론적 연구와 실증적 연구를 병행하여, 게이샤 관련 국내외 서적 및 관련기사를 통한 이론적 연구와 2010년 11월1일부터 11월30일간 교토를 방문하여 지온히가시(祇園東) 공연 관람을 통한 실증적 연구를 하였다. 연구범위는 이론적 배경으로 게이샤의 기원인 에도 시대(江戶時代, 1603-1876)부터 현재 2011년까지 게이샤의 등장배경 및 교육, 문화에 대해서 살펴보았다. 분석결과 게이샤의 메이크업 및 의상, 기타액세서리는 다음과 같은 특징을 나타내었다. 첫째, 메이크업에 있어서 흔히 아는 백색화장은 실제로 마이코나 나이어린 게이샤가 하는 화장법이며, 나이든 일반적인 게이샤는 평범한 화장을 한다는 사실을 알수 있었다. 특히 뒷목덜미의 헤어라인 부근의 ``니본아시(二本足)``와 ``산본아시(三本足)`` 그리고 작은 입술을 강조하는 ``오코보 구찌(Ochobo-guchi)는 게이샤 메이크업의 가장 중요한 의미를 내포하면서 여성미를 풍기는 가장 큰 특징이라 할 수 있겠다. 게이샤의 백색화장은 하얀 피부를 상징하는 신분상징의 표출과 함께 게이샤 등장 동시대인 서양의 로코코 시대 (17C)의 화장법과 그 유사점을 찾을 수 있었다. 둘째, 의상에 있어서는 일본의 전통문화를 그대로 보여주는 화려한 기모노를 착용한다는 점이며, 특히 예복으로 뒷목이 깊게 파이고 뒷자락이 끌리는 히키즈리 기모노(ヒキジュリ着物)를 착용함으로써 뒷목덜미의 메이크업이 좀더 잘 보일수 있도록 보조해주는 역할을 한다. 기타 액세서리에 있어서는 카수라 (Katsura)라는 가발의 사용의 증가 및 헤어를 장식하는 월별 화려한 간자시(カンジャシ), 신비하고 여성스럽게 보이도록 하는 오코보(オコボ)신발 등이 일본 문화자원으로서의 게이샤를 상품화시키기에 충분한 요소로서 자리잡고 있었다. 이렇듯 게이샤는 신비스럽기까지한 화장법과 일본전통 기모노 의상 및 액세서리를 통한 창조적 코디네이트를 함으로써 철저한 상품으로 포장되고 있으며, 1명당 연간 수입이 약 2천9백만엔(한화 4억원)이라는 막대한 경제적 가치를 창출하고 있다. 특히 문화행사 및 관광홍보를 통해 일본의 문화를 전 세계에 알리고 일본전통예술을 계승하는 측면에서 문화자원으로서의 가치를 재인식시키고 있는 것이다. The purpose of this research is to re-illuminate geisha as the economic value and cultural value by analyzing outside image with makeup, custom, and accessories on the base of geisha who can be called as flower of Japanese culture resource based on Kyoto Hanamachi. Theoretical and empirical researches were performed at the same time including theoretical research through national and international books and related articles regarding geisha and empirical research through visiting Gionhigasi(祇園東)performance during Kyoto visit from November 11, 2010 to November 30. The study range covers from Edo era(江戶時代, 1603-1876)to current 2011 background on the appearance of geisha, and education and culture for the theoretical background. In the result of analysis, geisha`s makeup and accessories have the following features. First, the white-makeup is actually for Mico or young geisha and old enough geisha does general makeup. Especially, Nibonasi(二本足) and Sanbonasi (三本足)at the part of hairline on the rear neck, and Ochobo-guchi strengthening the small lips contain the most significant meaning of geisha makeup and the most characteristic showing woman`s beauty. Geisha`s white makeup expresses status symbol representing white skin and has similarity of western rococo makeup which appeared at the same time with the appearance of geisha. Second, clothing as other accessories is to wear posh kimono showing Japanese traditional culture and especially by wearing Hikizeri(ヒキジュリ着物)with deep rear neck and dragging rear skirt as formal dress, the makeup of rear neck is strengthened. Besides, increasing use of wig called Katsura, fancy Ganjasi(カンジャシ)decorating hair, and Ocobo(オコボ)shoes strengthening mystery and feminine look stand as the enough elements to commercialize geisha for Japanese culture resource. Like this, by packaging geisha as the thorough commodity through creative coordination such as mysterious makeup and Japanese traditional kimono and accessories, and so on, they create tremendous economical value of about 29million yen(400 billion won) on yearly income per one person and advertise Japanese culture over the world and succeed to Japanese traditional arts through culture events and tourism advertisement, and impress on the importance of culture value of them.